Journal
BRITISH JOURNAL OF DERMATOLOGY
Volume 158, Issue 3, Pages 456-462Publisher
WILEY
DOI: 10.1111/j.1365-2133.2007.08393.x
Keywords
atopic dermatitis; T cells
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Funding
- Medical Research Council [G116/150, MC_U137881017] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
- Medical Research Council [MC_U137881017, G116/150] Funding Source: researchfish
- MRC [G116/150, MC_U137881017] Funding Source: UKRI
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Background Blockade of lymphocyte function associated antigen-1 (LFA-1) is proving successful in the management of psoriasis and other inflammatory skin conditions including atopic dermatitis (AD), but the dependence of allergen-specific CD4+ T-cell function on LFA-1 has not been studied extensively. Objectives We sought to investigate the potential ability of LFA-1 inhibition to influence keratinocyte presentation of allergen to specific T-helper (Th) 2 cell clones. Methods Using human leucocyte antigen class II tetrameric complexes, we generated Der p 1-specific DRB1*1501-restricted CD4+ T-cell lines (n = 5) and clones (n = 4) from the peripheral blood of five adults with AD. Results Using doses of anti-LFA-1 present in vivo, we observed significant inhibition (P < 0.05) of allergen-specific CD4+ T-cell production of interleukin-4 with such inhibition occurring during presentation of allergen by keratinocytes. Conclusion These data show that at doses present in vivo, LFA-1 blockade inhibits keratinocyte presentation to allergen-specific Th2 cells, suggesting one mechanism through which anti-LFA-1 may be beneficial therapeutically.
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