Article
Oncology
Kellen Mulford, Mariah McMahon, Andrew M. Gardeck, Matthew A. Hunt, Clark C. Chen, David J. Odde, Christopher Wilke
Summary: This study developed a non-invasive method to predict the motility of glioblastoma cells using radiomics features. It showed the feasibility of a quantitative MRI feature-based regression model in predicting the cellular motility of glioblastomas.
Article
Multidisciplinary Sciences
Meropi Bagka, Hyeonyi Choi, Margaux Heritier, Hanna Schwaemmle, Quentin T. L. Pasquer, Simon M. G. Braun, Leonardo Scapozza, Yibo Wu, Sascha Hoogendoorn
Summary: This study reveals that the cellular target of Hedgehog Pathway Inhibitor-1 is BET bromodomains by converting it into a bifunctional degrader. The strategy combines PROTACs and label-free quantitative proteomics to identify and validate the target, providing an effective approach for target deconvolution from phenotypic screens.
NATURE COMMUNICATIONS
(2023)
Article
Medicine, Research & Experimental
Shibo Zhang, Bingfan Xie, Lijie Wang, Hua Yang, Haopei Zhang, Yuming Chen, Feng Wang, Changqing Liu, Huanhuan He
Summary: The research revealed that M2 macrophages regulate the vascular barrier through the VCAM1/RAC1/ROS/p-PYK2/p-VE-cad cascade, providing specific therapeutic targets for the treatment of malignant ascites.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Oncology
Matteo Figini, Antonella Castellano, Michele Bailo, Marcella Callea, Marcello Cadioli, Samira Bouyagoub, Marco Palombo, Valentina Pieri, Pietro Mortini, Andrea Falini, Daniel C. Alexander, Mara Cercignani, Eleftheria Panagiotaki
Summary: The aim of this study was to expand the VERDICT-MRI framework to model brain tumors and characterize cellular and vascular features. Diffusion MRI data were collected from 21 patients with different types of brain tumors. Different diffusion models were fitted and compared based on their ability to characterize key tumor components. The best-performing model showed agreement with histology and had potential for differentiating tumor types and sub-regions.
Article
Biochemistry & Molecular Biology
Pawel Uruski, Justyna Mikula-Pietrasik, Eryk Naumowicz, Kamila Kazmierczak, Andrey N. Gaiday, Jan Krolak, Blazej Nowakowski, Rafal Moszynski, Andrzej Tykarski, Krzysztof Ksiazek
Summary: The cellular senescence phenotype in ovarian tumors shows a clear age-related pattern, with an increase in SA-β-Gal and γ-H2A.X expression associated with older age. Conversely, Ki67 levels decrease with age, particularly in patients with advanced disease and accumulating malignant ascites.
Article
Cell Biology
Jeffrey R. Johnson, David C. Crosby, Judd F. Hultquist, Andrew P. Kurland, Prithy Adhikary, Donna Li, John Marlett, Justine Swann, Ruth Huttenhain, Erik Verschueren, Tasha L. Johnson, Billy W. Newton, Michael Shales, Viviana A. Simon, Pedro Beltrao, Alan D. Frankel, Alexander Marson, Jeffery S. Cox, Oliver Fregoso, John A. T. Young, Nevan J. Krogan
Summary: Viruses remodel host cellular pathways to replicate and evade immune defenses and they do so with limited genomic coding capacity. This study used mass spectrometry-based proteomics to analyze protein abundance and post-translational modifications in response to HIV-1 infection. The results revealed the importance of Aurora kinase activity in HIV-1 infection and identified potential substrates of a degraded phosphatase during infection. Additionally, the study showed that the HIV-1 Vpr protein inhibits histone H1 ubiquitination, leading to DNA repair defects.
Article
Medicine, General & Internal
Liat Fellus-Alyagor, Inbal E. Biton, Hagit Dafni, Filip Bochner, Ron Rotkopf, Nava Dekel, Michal Neeman
Summary: This study characterized the early changes in blood vessel properties upon the establishment of dominance in the mouse ovary and investigated the vascular phenotype for prediction of ovulating follicles. The neo-vasculature during dominant follicle selection showed low blood volume and permeability, while the vasculature of the corpus luteum exhibited higher blood volume but similar permeability. The study showed that dominant follicles have relatively impermeable vasculature and delayed contrast enhancement in their antrum, which may aid in clinical prediction of follicular dominance and infertility treatment.
FRONTIERS IN MEDICINE
(2021)
Article
Microbiology
Nathan M. Good, Harvey D. Lee, Emily R. Hawker, Morgan Z. Su, Assaf A. Gilad, N. Cecilia Martinez-Gomez
Summary: Lanthanides (Ln), a new group of life metals, can be acquired and used by methylotrophic bacteria as cofactors with PQQ. A genetic variant of methylotrophic bacterium was found to efficiently accumulate heavy Ln and showed altered transcriptional response to heavy metal stress and methanol oxidation. The study also provided new insights into stress homeostasis and strategies for Ln recovery.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Melanie Borrmann, Florian Brandes, Benedikt Kirchner, Matthias Klein, Jean-Noel Billaud, Marlene Reithmair, Markus Rehm, Gustav Schelling, Michael W. Pfaffl, Agnes S. Meidert
Summary: COVID-19 infection leads to degradation of the endothelial protective glycocalyx layer, resulting in the release of major glycocalyx components. These circulating proteins and their degradation products can feedback on immune and endothelial cells, activating molecular signaling cascades associated with microvascular injury in COVID-19. The study found that plasma concentrations of glycocalyx components were highest in COVID-19 ARDS patients, and the degradation of endothelial glycocalyx components may lead to immune activation and more severe systemic inflammation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Toshihiko Oki, Francois Mercier, Hiroki Kato, Yookyung Jung, Thomas O. McDonald, Joel A. Spencer, Michael C. Mazzola, Nick van Gastel, Charles P. Lin, Franziska Michor, Toshio Kitamura, David T. Scadden
Summary: This study investigates the real-time dynamics of the mTORC1 pathway in relation to AML growth and response to chemotherapy using fluorescent markers, showing a decrease and subsequent increase in mTORC1 activity with AML progression and maximal chemotherapy response, respectively. Data also suggests that chemotherapy induces mTORC1 activity, and timed inhibition of mTORC1 enhances killing of AML cells. These findings provide insights for targeted intervention strategies in AML treatment.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Yueying Liu, Jing Yang, Zonggao Shi, Xuejuan Tan, Norman Jin, Catlin O'Brien, Connor Ott, Anna Grisoli, Eric Lee, Kelly Volk, Meghan Conroy, Emily Franz, Annamarie Bryant, Leigh Campbell, Brian Crowley, Stephen Grisoli, Aris T. Alexandrou, Chunyan Li, Elizabeth Harper, Marwa Asem, Jeff Johnson, Annemarie Leonard, Katie Santanello, Ashley Klein, Qingfei Wang, Siyuan Zhang, Tyvette S. Hilliard, M. Sharon Stack
Summary: The highly differentially expressed protein AMIGO2 in ovarian cancer metastasis could potentially serve as a therapeutic target, as its knockdown significantly attenuated the metastatic capacity of ovarian cancer cells.
Article
Neurosciences
Lisa Novello, Rafael Neto Henriques, Andrada Ianus, Thorsten Feiweier, Noam Shemesh, Jorge Jovicich
Summary: Diffusion MRI (dMRI) is an important imaging modality for studying tissue microstructure. This study implemented Correlation Tensor MRI (CTI) for the first time on a clinical 3T scanner and investigated the sources of total kurtosis in healthy subjects. The study found that microscopic kurtosis significantly contributes to diffusional kurtosis in grey and white matter tissue. The study also generated group average templates of kurtosis sources and discussed the potential future clinical applications of CTI.
Article
Biochemistry & Molecular Biology
Jiaxin Mi, Xu Chen, You Yiran, Yi Tang, Qiannan Liu, Jinghe Xiao, Wenhua Ling
Summary: This study revealed that elevated intracellular S-adenosyl-homocysteine (SAH) levels can induce vascular smooth muscle cell (VSMCs) senescence, leading to vascular aging. Inhibition of SAH hydrolase (SAHH) can trigger VSMCs senescence and demethylation of CpG island in the NF-kappa B promoter, promoting the pro-inflammatory response in senescent cells.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Anh Tran, Tong San Koh, Aldo Prawira, Rebecca Zhi Wen Ho, Thi Bich Uyen Le, Thanh Chung Vu, Septian Hartano, Xing Qi Teo, Way Cherng Chen, Philip Lee, Choon Hua Thng, Hung Huynh
Summary: This study aimed to evaluate the therapeutic effects of infigratinib and sorafenib on high-FGFR-expressing HCC xenografts using DCE-MRI, and found that infigratinib induced tumor regression while sorafenib caused growth arrest. DCE-MRI analysis showed different microenvironment changes between the two drugs, with infigratinib inducing early microcirculatory changes compared to sorafenib which mimicked rapidly growing tumors.
MOLECULAR IMAGING AND BIOLOGY
(2021)
Article
Oncology
Marion Tardieu, Yulia Lakhman, Lakhdar Khellaf, Maida Cardoso, Olivia Sgarbura, Pierre-Emmanuel Colombo, Mireia Crispin-Ortuzar, Evis Sala, Christophe Goze-Bac, Stephanie Nougaret
Summary: The study aimed to investigate the associations between MR microscopy (MRM) images and MRM-derived radiomic maps with histopathology in high-grade serous ovarian cancer (HGSOC). The results showed significant correlations between radiomic features and stromal proportion, and MRM was able to predict stromal proportion accurately using an automated model.
FRONTIERS IN ONCOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Kilian Horatz, Marco Giampa, Zhi Qiao, Siver A. Moestue, Franziska Lissel
Summary: MALDI MS and MSI are powerful analytical methods in medical and pathological research. Polymerizing SOMs into P(SOMs) mitigates background interferences, enhances vacuum stability, while maintaining the advantages of the original matrices.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Geriatrics & Gerontology
Irene Brusini, Eilidh MacNicol, Eugene Kim, Orjan Smedby, Chunliang Wang, Eric Westman, Mattia Veronese, Federico Turkheimer, Diana Cash
Summary: The study suggests that interventions related to lifestyle factors can slow down brain aging in rodents, showing that long-term environmental enrichment and dietary restriction can lead to a slower increase in BrainAGE in rats. Higher BrainAGE at 5 months was found to be associated with increased mortality risk, highlighting the potential of BrainAGE as a predictor of age-related health outcomes.
NEUROBIOLOGY OF AGING
(2022)
Article
Mathematical & Computational Biology
Eilidh MacNicol, Paul Wright, Eugene Kim, Irene Brusini, Oscar Esteban, Camilla Simmons, Federico E. Turkheimer, Diana Cash
Summary: Age-specific resources in human and rat MRI are important for mitigating processing biases and accurately estimating tissue volumes. This study found that by using age-specific rat brain templates and tissue probability maps, better estimations of gray matter volumes could be achieved. Furthermore, it was discovered that tissue volumes and proportions relative to total brain volume vary across adulthood in rats.
FRONTIERS IN NEUROINFORMATICS
(2022)
Article
Multidisciplinary Sciences
Cigdem Gelegen, Diana Cash, Katarina Ilic, Millie Sander, Eugene Kim, Camilla Simmons, Michel Bernanos, Joana Lama, Karen Randall, Jonathan T. Brown, Svjetlana Kalanj-Bognar, Samuel Cooke, K. Ray Chaudhuri, Clive Ballard, Paul Francis, Ivana Rosenzweig
SCIENTIFIC REPORTS
(2022)
Review
Neurosciences
Maria Elisa Serrano, Eugene Kim, Marija M. Petrinovic, Federico Turkheimer, Diana Cash
Summary: The brain is a complex organ composed of billions of neurons and trillions of synapses. Proper regulation of synaptic density is crucial for normal brain development, and abnormalities in synapses have been associated with various neurological and psychiatric disorders. Several methods, including PET and MRI, are being developed to measure synaptic density, but further research is needed to address their limitations before they can be used clinically.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Biophysics
Rachel L. C. Barrett, Diana Cash, Camilla Simmons, Eugene Kim, Tobias C. Wood, Richard Stones, Anthony C. Vernon, Marco Catani, Flavio Dell'Acqua
Summary: In this study, an optimization strategy for ex vivo diffusion imaging was developed to improve image quality and signal-to-noise ratio (SNR). The strategy successfully enabled the acquisition of high-resolution diffusion data with improved SNR efficiency and allowed for faster acquisitions in preclinical experiments.
NMR IN BIOMEDICINE
(2023)
Article
Psychiatry
Amanda Kiemes, Maria Elisa Serrano Navacerrada, Eugene Kim, Karen Randall, Camilla Simmons, Loreto Rojo Gonzalez, Marija-Magdalena Petrinovic, David J. Lythgoe, Diana Rotaru, Davide Di Censo, Lydiane Hirschler, Emmanuel L. Barbier, Anthony C. Vernon, James M. Stone, Cathy Davies, Diana Cash, Gemma Modinos
Summary: This study found that specific disruption of cortical inhibitory interneurons in mice can reproduce some of the neuroimaging findings in patients with psychosis, linking inhibitory interneuron deficits to non-invasive measures of brain function and neurochemistry that can be used across species.
SCHIZOPHRENIA BULLETIN
(2023)
Article
Biology
Jadna Bogado Lopes, Anna N. Senko, Klaas Bahnsen, Daniel Geisler, Eugene Kim, Michel Bernanos, Diana Cash, Stefan Ehrlich, Anthony C. Vernon, Gerd Kempermann
Summary: By continuously tracking the behavioral activity of mice in an enriched environment for 3 months and using ex vivo structural magnetic resonance imaging, it was found that behavioral activity can shape the connectivity of the mouse brain. Furthermore, there are differences in brain structural covariance between mice with different behavioral trajectories.
Article
Cell Biology
Apsana Lamsal, Sonja Benedikte Andersen, Ida Johansson, Marina Vietri, Ansooya Avinash Bokil, Natalie Jayne Kurganovs, Felicia Rylander, Geir Bjorkoy, Kristine Pettersen, Miriam S. S. Giambelluca
Summary: Solid tumors rely on suppressed local immune reactions in the tumor microenvironment, and the formation of immunosuppressive tumor microenvironments is still not fully understood. This study found that the type I interferon response is dampened in tumors with metastatic ability, and decreased expression of IFN-I-related genes predicts poor prognosis in breast cancer patients.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Matthew R. Bruce, Amalie C. M. Couch, Simone Grant, Janna McLellan, Katherine Ku, Christina Chang, Angelica Bachman, Matthew Matson, Robert F. Berman, Richard J. Maddock, Douglas Rowland, Eugene Kim, Matthew D. Ponzini, Danielle Harvey, Sandra L. Taylor, Anthony C. Vernon, Melissa D. Bauman, Judy van de Water
Summary: Maternal immune dysregulation is a prenatal risk factor for autism spectrum disorder (ASD). Maternal autoantibodies (aAbs) may disrupt metabolic signaling and induce neuroanatomical changes in the brains of exposed offspring. Rats exposed to MAR-ASD aAbs present with alterations in behavior, brain structure, and neurometabolites, reminiscent of findings observed in clinical ASD.
MOLECULAR PSYCHIATRY
(2023)
Article
Neurosciences
Joanes Grandjean, Gabriel Desrosiers-Gregoire, Cynthia Anckaerts, Diego Angeles-Valdez, Fadi Ayad, David A. Barriere, Ines Blockx, Aleksandra Bortel, Margaret Broadwater, Beatriz M. Cardoso, Marina Celestine, Jorge E. Chavez-Negrete, Sangcheon Choi, Emma Christiaen, Perrin Clavijo, Luis Colon-Perez, Samuel Cramer, Tolomeo Daniele, Elaine Dempsey, Yujian Diao, Arno Doelemeyer, David Dopfel, Lenka Dvorakova, Claudia Falfan-Melgoza, Francisca F. Fernandes, Caitlin F. Fowler, Antonio Fuentes-Ibanez, Clement Garin, Eveline Gelderman, Carla E. M. Golden, Chao C. G. Guo, Marloes J. A. G. Henckens, Lauren A. Hennessy, Peter Herman, Nita Hofwijks, Corey Horien, Tudor M. Ionescu, Jolyon Jones, Johannes Kaesser, Eugene Kim, Henriette Lambers, Alberto Lazari, Sung-Ho Lee, Amanda Lillywhite, Yikang Liu, Yanyan Y. Liu, Alejandra Lopez-Castro, Xavier Lopez-Gil, Zilu Ma, Eilidh MacNicol, Dan Madularu, Francesca Mandino, Sabina Marciano, Matthew J. McAuslan, Patrick McCunn, Alison McIntosh, Xianzong Meng, Lisa Meyer-Baese, Stephan Missault, Federico Moro, Daphne M. P. Naessens, Laura J. Nava-Gomez, Hiroi Nonaka, Juan J. Ortiz, Jaakko Paasonen, Lore M. Peeters, Mickael Pereira, Pablo D. Perez, Marjory Pompilus, Malcolm Prior, Rustam Rakhmatullin, Henning M. Reimann, Jonathan Reinwald, Rodrigo Triana Del Rio, Alejandro Rivera-Olvera, Daniel Ruiz-Perez, Gabriele Russo, Tobias J. Rutten, Rie Ryoke, Markus Sack, Piergiorgio Salvan, Basavaraju G. Sanganahalli, Aileen Schroeter, Bhedita J. Seewoo, Erwan Selingue, Aline Seuwen, Bowen Shi, Nikoloz Sirmpilatze, Joanna A. Smith, Corrie Smith, Filip Sobczak, Petteri J. Stenroos, Milou Straathof, Sandra Strobelt, Akira Sumiyoshi, Kengo Takahashi, Maria E. Torres-Garcia, Raul Tudela, Monica van den Berg, Kajo van der Marel, Aran T. B. van Hout, Roberta Vertullo, Benjamin Vidal, Roel M. Vrooman, Victora X. Wang, Isabel Wank, David J. G. Watson, Ting Yin, Yongzhi Zhang, Stefan Zurbruegg, Sophie Achard, Sarael Alcauter, Dorothee P. Auer, Emmanuel L. Barbier, Juergen Baudewig, Christian F. Beckmann, Nicolau Beckmann, Guillaume J. P. C. Becq, Erwin L. A. Blezer, Radu Bolbos, Susann Boretius, Sandrine Bouvard, Eike Budinger, Joseph D. Buxbaum, Diana Cash, Victoria Chapman, Kai-Hsiang Chuang, Luisa Ciobanu, Bram F. Coolen, Jeffrey W. Dalley, Marc Dhenain, Rick M. Dijkhuizen, Oscar Esteban, Cornelius Faber, Marcelo Febo, Kirk W. Feindel, Gianluigi Forloni, Jeremie Fouquet, Eduardo A. Garza-Villarreal, Natalia Gass, Jeffrey C. Glennon, Alessandro Gozzi, Olli Grohn, Andrew Harkin, Arend Heerschap, Xavier Helluy, Kristina Herfert, Arnd Heuser, Judith R. Homberg, Danielle J. Houwing, Fahmeed Hyder, Giovanna Diletta Ielacqua, Ileana O. Jelescu, Heidi Johansen-Berg, Gen Kaneko, Ryuta Kawashima, Shella D. Keilholz, Georgios A. Keliris, Clare Kelly, Christian Kerskens, Jibran Y. Khokhar, Peter C. Kind, Jean-Baptiste Langlois, Jason P. Lerch, Monica A. Lopez-Hidalgo, Denise Manahan-Vaughan, Fabien Marchand, Rogier B. Mars, Gerardo Marsella, Edoardo Micotti, Emma Munoz-Moreno, Jamie Near, Thoralf Niendorf, Willem M. Otte, Patricia Pais-Roldan, Wen-Ju Pan, Roberto A. Prado-Alcala, Gina L. Quirarte, Jennifer Rodger, Tim Rosenow, Cassandra Sampaio-Baptista, Alexander Sartorius, Stephen J. Sawiak, Tom W. J. Scheenen, Noam Shemesh, Yen-Yu Ian Shih, Amir Shmuel, Guadalupe Soria, Ron Stoop, Garth J. Thompson, Sally M. Till, Nick Todd, Annemie van der Linden, Annette van der Toorn, Geralda A. F. van Tilborg, Christian Vanhove, Andor Veltien, Marleen Verhoye, Lydia Wachsmuth, Wolfgang Weber-Fahr, Patricia Wenk, Xin Yu, Valerio Zerbi, Nanyin Zhang, Baogui B. Zhang, Luc Zimmer, Gabriel A. Devenyi, M. Mallar Chakravarty, Andreas Hess
Summary: Task-free functional connectivity in animal models is an important tool for studying connectivity phenomena. However, the lack of standardized protocols and analysis methods hampers result comparison and integration. In this study, the authors developed a standardized protocol, called StandardRat, for rat functional magnetic resonance imaging (fMRI) acquisition and analysis, which enhances the detection of functional connectivity patterns and promotes cooperation in neuroscience research.
NATURE NEUROSCIENCE
(2023)
Article
Immunology
Eugene Kim, Ines Carreira Figueiredo, Camilla Simmons, Karen Randall, Loreto Rojo Gonzalez, Tobias Wood, Brigida Ranieri, Paula Sureda-Gibert, Oliver Howes, Carmine Pariante, N. I. M. A. NIMA Consortium, Ofer Pasternak, Flavio Dell'Acqua, Federico Turkheimer, Diana Cash
Summary: It has been found that neuroinflammation is crucial in various neurological and psychiatric disorders. Recent studies have shown that diffusion MRI (dMRI) can detect changes in microglial density and morphology related to neuroinflammation, but these studies were mostly conducted ex vivo or in extreme animal models. In this study, the utility of established dMRI methods to detect neuroinflammation in a clinically relevant animal model of sickness behavior was investigated. The results demonstrated that diffusion tensor imaging (DTI) and neurite orientation dispersion and density imaging (NODDI) indicated widespread increases in diffusivity in the brains of rats with a lipopolysaccharide challenge. These findings confirm the sensitivity of dMRI to neuroinflammatory processes and contribute to the development of a noninvasive indicator of neuroinflammation for early diagnosis and treatment monitoring in neurological and psychiatric diseases.
BRAIN BEHAVIOR AND IMMUNITY
(2023)
Article
Chemistry, Inorganic & Nuclear
Cinzia Imberti, Jamie Lok, James P. C. Coverdale, Oliver W. L. Carter, Millie E. Fry, Miles L. Postings, Jana Kim, George Firth, Philip J. Blower, Peter J. Sadler
Summary: A novel photoactivatable Pt(IV) diazido anticancer agent with a pendant deferoxamine (DFO) siderophore for radiometal chelation was synthesized, and its in vivo behavior was studied using radionuclide imaging. The results showed that the Pt-succ-DFO complex had photoactivation properties and photocytotoxicity in cancer cells. PET imaging in healthy mice exhibited a promising biodistribution profile, suggesting the potential of this agent for clinical development. This research provides important insights into the development of light-activated agents.
INORGANIC CHEMISTRY
(2023)
Article
Oncology
Ansooya A. Bokil, Mathieu Le Boulvais Borkja, Camilla Wolowczyk, Apsana Lamsal, Wenche S. Prestvik, Unni Nonstad, Kristine Pettersen, Sonja B. Andersen, Anna M. Bofin, Geir Bjorkoy, Sjoerd Hak, Miriam S. Giambelluca
Summary: This study identified a unique expression pattern of arginase 1 (ARG1) in myeloid cells, which is correlated with the metastatic capacity of breast cancer. The presence of ARG1-positive cells in human breast cancer biopsies is strongly associated with high-grade proliferating tumors, poor prognosis, and low survival.
CANCER CELL INTERNATIONAL
(2023)
Meeting Abstract
Obstetrics & Gynecology
Z. Wang, A. Van der Veen, H. Groen, A. E. P. Cantineau, A. Van Oers, J. M. Hawley, B. G. Keevil, H. J. R. Van Faassen, I. P. Kema, A. Hoek
HUMAN REPRODUCTION
(2022)
