Article
Biophysics
Sen Liu, Haojun Liang, Linwen Lv, Fan Hu, Qiuyang Liu, Yujiao Wang, Junyu Zhu, Ziteng Chen, Jiacheng Li, Zhijie Wang, Ya-nan Chang, Juan Li, Xiancai Ma, Kui Chen, Gengmei Xing
Summary: This study found that the polyhydroxylated fullerenol nanoparticle (FNP) can induce the exposure of calreticulin (CRT) on the surface of MCF-7 cells by redistributing CRT from the endoplasmic reticulum (ER). The combination of FNP and anti-CD47 monoclonal antibody (mAb) enhances macrophage-mediated phagocytosis of cancer cells. In vivo experiments also confirmed the regulatory effect of FNP on the progression of MCF-7 cancer stem-like cells (CSCs). These findings expand the application of FNP in tumor therapy with anti-CD47 mAb and suggest the use of 3D culture as a screening tool for nanomedicine.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Neurosciences
Yanhao Chu, Shilin Jia, Ke Xu, Qing Liu, Lijia Mai, Jiawei Liu, Wenguo Fan, Fang Huang
Summary: This study reveals the transcript characteristics and heterogeneity of satellite glial cells (SGCs) in trigeminal ganglion (TG) and investigates the interactions between SGCs and neurons. The results show that TG consists of various cell clusters including neurons, SGCs, myeloid Schwann cells, non-myeloid Schwann cells, and immune cells, with corresponding markers identified. The findings provide valuable insights into studying SGCs in the TG.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Cell Biology
Kamila Bialkowska, Piotr Komorowski, Rafael Gomez-Ramirez, Francisco Javier de la Mata, Maria Bryszewska, Katarzyna Milowska
Summary: Cationic dendrimers have been found to be effective carriers for delivering siRNA into cells and protecting nucleic acids. In 3D cell cultures, CBD-2 exhibits higher cytotoxicity compared to CBD-1, while the opposite is observed in 2D cultures. Attaching siRNA to a dendrimer molecule does not increase cellular toxicity. CBD-2 shows higher efficiency in transporting siRNAs compared to CBD-1.
Article
Chemistry, Analytical
Ayman A. Abdelrahim, Sera Hong, Joon Myong Song
Summary: A three-dimensional bioprinted cancer spheroid-embedded hydrogel model was proposed to evaluate the efficacy of anticancer drugs. The model successfully measured the fluorescence intensities of tumor spheroids in situ, without the need for separate measurements. Compared to two-dimensional cultures, the hydrogel model showed higher drug resistance and heterogeneous response, mimicking the tumor microenvironment.
ANALYTICAL CHEMISTRY
(2022)
Article
Biology
Hareth Y. ShujaaEdin, Nagi A. AL-Haj, Abdullah Rasedee, Noorjahan Banu Alitheen, Arifah Abdul Kadir, Chee Wun How, Heshu Sulaiman Rahman, Al-Shwyeh Hussah Abdullah
Summary: The study found that rHuEPO had a greater effect on the viability of MCF-7 cells in 3D culture, significantly reducing cell viability and down-regulating caspase activity. Cell cycle analysis showed that rHuEPO caused the MCF-7 cells to enter the subG0/G1 phase.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Cell Biology
Giuseppe Garroni, Francesca Balzano, Sara Cruciani, Renzo Pala, Donatella Coradduzza, Emanuela Azara, Emanuela Bellu, Maria Laura Cossu, Giorgio C. Ginesu, Ciriaco Carru, Carlo Ventura, Margherita Maioli
Summary: The exhausted medium from MCF-7 influences the behavior of hADSCs by increasing the expression of stemness-related genes, cell proliferation, and autophagy. Polyamines detectable in the MCF-7 exhausted medium may be related to the higher proliferation capability observed in hADSCs, suggesting a direct crosstalk between these molecules and the changes in stem cell potency.
Article
Neurosciences
Oshri Avraham, Pan-Yue Deng, Dario Maschi, Vitaly A. Klyachko, Valeria Cavalli
Summary: Research on sensory deficits in FXS suggests that the disrupted association between neurons and peripheral glial cells may lead to changes in neuronal maturation and glial vesicular secretion, potentially contributing to sensory deficits.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Chunjie Ma, Yu Wang, Wei Chen, Ting Hou, Honglian Zhang, Hongguang Zhang, Xu Yao, Chunhui Xia
Summary: This study examined the effects of TaPcZn-PDT on cytotoxicity, cell viability, apoptosis, pyroptosis, cellular reactive oxygen species (ROS), mitochondrial membrane potential (??m), caspase-1, caspase-3, and nuclear transcription factor-?B (NF?B) in MCF-7 cells. The results showed that TaPcZn-PDT increased cytotoxicity and the percentage of apoptotic and pyroptotic cells, decreased cell viability and ??m, induced ROS production, and activated caspase-1, caspase-3, and NF?B in MCF-7 cells. siRNA targeting caspase-1 attenuated the effects of TaPcZn-PDT on apoptosis, pyroptosis, and the activation of caspase-1, caspase-3, and NF?B in MCF-7 cells. Therefore, caspase-1 plays a regulatory role in the apoptosis and pyroptosis induced by TaPcZn-PDT in MCF-7 cells.
Article
Biochemistry & Molecular Biology
Jyh Yea Chia, Takayuki Miki, Hisakazu Mihara, Hiroshi Tsutsumi
Summary: Short self-assembling peptide (FFiK)(2) and its derivatives can be functionalized by conjugating with various bioactive sequences to facilitate 3D culture of cancer cells. These functionalized peptide hydrogels can encapsulate MCF-7 cells without significant cytotoxicity, promoting their proliferation and potentially serving as a platform for drug screening targeting cancer stem cells.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Dilek Bayram, Meltem ozgocmen, Dilek Asci celik, Emine Sarman, Murat Sevimli
Summary: Breast cancer, originating in the lobules or ducts of the breast, is the second leading cause of death among women worldwide. Studies have been conducted to find the optimal treatment for breast cancer, and boric acid is considered as a potential anti-cancer agent for treating this disease.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Na Wei, Ya-Ping Liu, Rui-Rui Wang, Zhen-Juan Zhong, Xiao-Liang Wang, Yan Yang, Ting He, Si-Jia Zhao, Huan Wang, Yao-Qing Yu
Summary: This study investigated the effects of different culture supplementations on the proliferation and survival of satellite glial cells (SGCs). It was found that glutamine in DMEM/F12 medium played a crucial role in maintaining the growth and survival of SGCs. Changing the key component of the culture medium could lead to the generation of high-purity SGCs.
NEUROCHEMICAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Lilian Areal Marques, Simone Cristine Semprebon, Bruna Isabela Biazi, Ingrid Felicidade, Thalita Alves Zanetti, Adrivanio Baranoski, Virginia Marcia Concato, Wander Rogerio Pavanelli, Mario Sergio Mantovani
Summary: This study demonstrates the synergistic cytotoxic and antiproliferative effects of Salinomycin and 1,25D on MCF-7 breast carcinoma cells. The combination of these two drugs activates multiple cell death pathways and inhibits cell proliferation, suggesting a potential novel therapy strategy for breast cancer.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2022)
Article
Biology
Yu Wang, Meghan Lee Arnold, Anna Joelle Smart, Guoqiang Wang, Rebecca J. Androwski, Andres Morera, Ken C. Q. Nguyen, Peter J. Schweinsberg, Ge Bai, Jason Cooper, David H. Hall, Monica Driscoll, Barth D. Grant, Paschalis Kratsios
Summary: Research reveals that exophers produced by mechanosensory neurons in C. elegans are engulfed by surrounding hypodermal skin cells and broken up into smaller vesicles, which are gradually degraded by hypodermal lysosomes. This process relies on factors and pathways in the hypodermis, including actin and molecules related to lysosome activity. Furthermore, specific interaction between the neuron and phagocyte is crucial for an efficient exopher response.
Article
Toxicology
Juliana Mara Serpeloni, Larissa Cristina Bastos de Oliveira, Andressa Fujiike, Katiuska Tuttis, Diego Luis Ribeiro, Marcos Bispo Pinheiro Camara, Claudia Quintino da Rocha, Ilce Mara de Syllos Colus
Summary: This study investigated the mechanisms underlying the antitumoral activity of cirsimarin on MCF-7 cells. Cirsimarin decreased cell viability and colony formation, induced cell death and reduced spheroid integrity. It also inhibited invasion and migration, and induced DNA damage. The antitumoral effects of cirsimarin were mediated by negative modulation of genes associated with cell proliferation and death. These findings have important implications for preventing metastasis and improving the prognosis of breast cancer.
TOXICOLOGY IN VITRO
(2022)
Article
Biochemistry & Molecular Biology
Gozde Koygun, Emine Arslan, Gokhan Zengin, Giustino Orlando, Claudio Ferrante
Summary: The study assessed the cytotoxicity of Dorycnium pentaphyllum subsp. haussknechtii against breast cell lines and found significant antiproliferative effects, particularly attributed to quercetin. The extract effectively induced tumor cell death without cytotoxicity to healthy cells. These findings suggest that the extraction of D. pentaphyllum subsp. haussknechtii could be a promising strategy to isolate biomolecules with cytotoxic effects against breast cancer cells, with quercetin playing a key role in this process.
Article
Oncology
Davide Maffei, Francesco Giganti, Caroline M. Moore
Summary: Prostate cancer is the most common cancer in men in Europe and many countries worldwide, and the second leading cause of cancer-related death. This article discusses the advances in MRI imaging and its potential role as a screening tool for prostate cancer, considering whether MRI can be used before biopsy or as a primary screening method.
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
(2023)
Article
Engineering, Biomedical
Simon C. Kellaway, Victoria Roberton, Joshua N. Jones, Rabea Loczenski, James B. Phillips, Lisa J. White
Summary: Incorporating dECMh derived from bone, liver, and small intestinal tissues can promote neurite extension and axonal regeneration, providing an alternative to current collagen I hydrogel.
ACTA BIOMATERIALIA
(2023)
Editorial Material
Urology & Nephrology
Alexander P. Cole, Mara Koelker, Jonathan Makanjuola, Caroline M. Moore
Summary: Growing evidence suggests worse urinary and sexual functional outcomes for Black men after radical prostatectomy for prostate cancer. Incorporating patient-reported outcomes measures into patient care could improve equity in treatment outcomes for men with prostate cancer.
NATURE REVIEWS UROLOGY
(2023)
Correction
Urology & Nephrology
Alexander P. Cole, Mara Koelker, Jonathan Makanjuola, Caroline M. Moore
NATURE REVIEWS UROLOGY
(2023)
Article
Medicine, General & Internal
Hannah Warren, Thomas Wagner, Michael A. Gorin, Steven Rowe, Beverley Fiona Holman, Deborah Pencharz, Soha El-Sheikh, Ravi Barod, Prasad Patki, Faiz Mumtaz, Axel Bex, Veeru Kasivisvanathan, Caroline M. Moore, Nicholas Campain, Jon Cartledge, Andrew Scarsbrook, Fahim Hassan, Tim S. O'Brien, Grant D. Stewart, Iosif Mendichovszky, Sabina Dizdarevic, Ammar Alanbuki, William H. Wildgoose, Tze Wah, Cecilia Vindrola-Padros, Elena Pizzo, Hakim-Moulay Dehbi, Paula Lorgelly, Kurinchi Gurusamy, Mark Emberton, Maxine G. B. Tran
Summary: The incidence of renal tumours is increasing and anatomic imaging cannot reliably distinguish benign tumours from renal cell carcinoma. Tc-99m-sestamibi SPECT/CT has shown high diagnostic accuracy for benign renal oncocytomas and other oncocytic renal neoplasms of low malignant potential in single-centre studies. The primary aim of MULTI-MIBI is to assess feasibility of a multicentre study of Tc-99m-sestamibi SPECT/CT against a reference standard of histopathology from surgical resection or biopsy.
Article
Biochemistry & Molecular Biology
Zhong Huang, Rebecca Powell, Svenja Kankowski, James B. Phillips, Kirsten Haastert-Talini
Summary: Researchers have successfully generated Schwann cells from human induced pluripotent stem cells by modifying existing protocols, resulting in sufficient cell numbers. They also found that specific coating and glucose levels are crucial for improving the differentiation process and increasing the yield of viable cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Layla Mohammad Hadi, Katerina Stamati, Elnaz Yaghini, Alexander J. MacRobert, Marilena Loizidou
Summary: Photochemical internalisation (PCI) is a method that uses sub-lethal photodynamic therapy (PDT) to control cytosolic drug delivery, activating a photosensitiser with non-ionising visible light. This study examines the efficacy of PCI on ovarian cancer tumouroids using a porphyrin photosensitiser and a cytotoxin (Saporin), showing that PCI effectively decreases cancer cell viability within the central cancer mass and the surrounding stroma. Compared to treatment with Saporin or PDT alone, PCI significantly reduces stromal invasion by cancer cells. Tumouroids are suitable models for studying multi-cellular events after pharmacological assault.
Article
Radiology, Nuclear Medicine & Medical Imaging
Saurabh Singh, Francesco Giganti, Louise Dickinson, Harriet Rogers, Baris Kanber, Joey Clemente, Hayley Pye, Susan Heavey, Urszula Stopka-Farooqui, Edward W. Johnston, Caroline M. Moore, Alex Freeman, Hayley C. Whitaker, Daniel C. Alexander, Eleftheria Panagiotaki, Shonit Punwani
Summary: This study aimed to assess the image quality of ADC and FIC maps derived from conventional diffusion-weighted MRI and VERDICT MRI in patients from the INNOVATE trial. The results showed that the image quality was comparable between FIC and ADC, but the image quality of ADC was higher than FIC when assessed using the Likert score.
EUROPEAN JOURNAL OF RADIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Owein Guillemot-Legris, Gedion Girmahun, Rebecca J. Shipley, James B. Phillips
Summary: Peripheral nerve injuries are common and often require surgery, but patients often do not regain satisfactory sensory and motor functions. This causes a heavy socioeconomic burden. We investigated the effects of locally delivering minocycline on nerve regeneration and found that it improved the outcome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Radiology, Nuclear Medicine & Medical Imaging
Alexandre Woernle, Cameron Englman, Louise Dickinson, Alex Kirkham, Shonit Punwani, Aiman Haider, Alex Freeman, Veeru Kasivisivanathan, Mark Emberton, John Hines, Caroline M. Moore, Clare Allen, Francesco Giganti
Summary: Magnetic resonance imaging is the gold standard for diagnosing prostate cancer. This critical review focuses on improving and assessing image quality, exploring the role of patient preparation, scanner factors, and more advanced sequences including Artificial Intelligence. The review also evaluates scoring systems and discusses the application of AI and teaching courses for quality assessment.
JOURNAL OF MAGNETIC RESONANCE IMAGING
(2023)
Article
Multidisciplinary Sciences
Maxime Berg, Despoina Eleftheriadou, James B. Phillips, Rebecca J. Shipley
Summary: Cellular engineered neural tissues have the potential to improve peripheral nerve repair strategies. Mathematical cell-solute models benchmarked against experimental data enable computational experiments, accelerating the development of new treatment strategies.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2023)
Article
Materials Science, Multidisciplinary
Ryan P. Trueman, Peter Gilhooly Finn, Megan M. Westwood, Avishek Dey, Robert Palgrave, Alethea Tabor, James B. Phillips, Bob C. Schroeder
Summary: This study investigates the effects of molecular doping on the surface properties of polydiketopyrrolopyrrole terthiophene (DPP3T) thin films and its subsequent impact on Schwann cell behavior. The results demonstrate that molecular doping alters the topography of DPP3T films, leading to increased cell numbers and larger, more spread out cell morphology. Furthermore, the viability of the cells on the doped films remains unaffected.
JOURNAL OF MATERIALS CHEMISTRY C
(2023)
Article
Urology & Nephrology
Mara Koelker, Muhieddine Labban, Nicola Frego, Christian P. Meyer, Georg Salomon, Stuart R. Lipsitz, John Withington, Caroline M. Moore, Clare M. Tempany, Kemal Tuncali, Arvin George, Adam S. Kibel, Quoc-Dien Trinh, Alexander P. Cole
Summary: This study aimed to describe the national patterns of care for local ablative therapy among men with prostate cancer and identify factors associated with the receipt of these techniques. The National Cancer Database was used to retrospectively study men with clinically localized prostate cancer between 2010 and 2017. The results showed that the use of local tumor ablation was associated with patient age, disease risk, insurance, and was less likely to be performed in academic/research hospitals.
WORLD JOURNAL OF UROLOGY
(2023)
Review
Medicine, Research & Experimental
Simao Laranjeira, Victoria H. H. Roberton, James B. B. Phillips, Rebecca J. J. Shipley
Summary: Drug therapies for peripheral nerve injury repair have shown promise in preclinical studies, but are not routinely used clinically. Systemic drug delivery often leads to adverse effects, making it challenging to determine effective concentration and delivery strategy. Mathematical modeling can inform transport mechanisms and predict the efficacy of local delivery protocols, accelerating the development of drug delivery approaches for peripheral nerve injury repair.
WIRES MECHANISMS OF DISEASE
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Francesco Giganti, Alexander P. Cole, Fiona M. Fennessy, Timothy Clinton, Pedro Lopes Da Frota Moreira, Mariana Costa Bernardes, Carl-Fredrik Westin, Deepa Krishnaswamy, Andriy Fedorov, Daniel A. Wollin, Bjoern Langbein, Nicola Frego, Muhieddine Labban, Joy S. Badaoui, Steven L. Chang, Logan G. Briggs, Junichi Tokuda, Alessandro Ambrosi, Alex Kirkham, Mark Emberton, Veeru Kasivisvanathan, Caroline M. Moore, Clare Allen, Clare M. Tempany
Summary: This study assessed the impact of a prostate MRI quality training lecture on the participants' ability to apply the PI-QUAL scoring system. The results showed that the teaching course significantly improved the participants' ability to evaluate the quality of prostate MRI examinations using the PI-QUAL score.
EUROPEAN RADIOLOGY
(2023)