Article
Chemistry, Analytical
Jiani Li, Fanfan Liang, Li Han, Xiaoxuan Yu, Dingbin Liu, Wensheng Cai, Xueguang Shao
Summary: In this study, the characteristic absorption of water in the near-infrared (NIR) region was utilized to determine extra-cellular pH (pHe) and intra-cellular pH (pHi). The use of continuous wavelet transform (CWT) enhanced the resolution of the spectra and partial least squares (PLS) regression was employed to establish quantitative models for pHe and pHi. The results showed that water with different hydrogen bonds can serve as a good probe to sense pH within biological systems.
Article
Oncology
Carolyn Shembrey, Jai Smith, Melodie Grandin, Nathalia Williams, Hyun-Jung Cho, Christina Molck, Corina Behrenbruch, Benjamin N. J. Thomson, Alexander G. Heriot, Delphine Merino, Frederic Hollande
Summary: Colorectal cancer is the second most common cancer worldwide, and tumor heterogeneity affects treatment outcome. This study presents a multi-color barcoding method for real-time quantification of tumor heterogeneity. The results show limited heterogeneity in traditional cell culture models and better representation of heterogeneity in patient-derived organoids. The optimized imaging protocol allows for real-time tracking of clonal dynamics. In summary, this technique is of great importance for monitoring tumor heterogeneity in cell culture models.
Article
Oncology
Xiaoqian Liu, Wenqian Wang, Xiaoding Liu, Zhiwen Zhang, Lianyuan Yu, Ruiyu Li, Dan Guo, Weijing Cai, Xueping Quan, Huanwen Wu, Menghua Dai, Zhiyong Liang
Summary: This study analyzed the intra- and inter-tumoural heterogeneity of PDAC and found diverse mutations and copy number variations within and between tumours. Integration analysis of multi-omics data identified potential genes associated with PDAC and may guide personalized treatment strategies in PDAC therapy.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Review
Chemistry, Multidisciplinary
Weikun Huang, Tongzhi Wu, Cong Xie, Christopher K. Rayner, Craig Priest, Heike Ebendorff-Heidepriem, Jiangbo (Tim) Zhao
Summary: The article discusses the importance of calcium ions in intra- and inter-cellular signaling, particularly in insulin secretion. Various sensors for detecting calcium ions are described, with a focus on emerging approaches and challenges in the field.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Yufei Yan, Jiefei Zeng, Linhao Xing, Changyong Li
Summary: Hepatic fibrosis is characterized by the accumulation of extracellular matrix in the liver due to persistent injury. Understanding the cellular and molecular mechanisms controlling the fibrotic response is crucial for developing clinical strategies to prevent fibrosis progression. Activation of hepatic stellate cells plays a key role in promoting ECM synthesis, and various external signals contribute to this process.
Article
Cell Biology
Christopher Walter, Jairaj Mathur, Amit Pathak
Summary: Adherent cells migrate on layered tissue interfaces to drive morphogenesis, wound healing, and tumor invasion. Stiffer surfaces enhance cell migration, but it is unclear if cells can sense stiff environments buried under softer matrix. Using collagen-polyacrylamide gels, we found that cancer cells with stiff base matrix generate protrusions and faster migration due to depth mechanosensing, which is disrupted by polarity disruption or inhibition of contractility.
Article
Chemistry, Physical
Tengjisi, Yun Liu, Da Zou, Guangze Yang, Chun-Xia Zhao
Summary: This study synthesized two types of NPs using the dual-functional peptide SurSi for the first time to investigate the drug release kinetics. The results showed that cargo diffusion dominated the release during circulation, while the degradation of the silica shell played a key role in drug release intracellularly.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Cell Biology
Sneha Ratnapriya, Miranda Harris, Angela Chov, Zachary T. Herbert, Vladimir Vrbanac, Maud Deruaz, Vasudevan Achuthan, Alan N. Engelman, Joseph Sodroski, Alon Herschhorn
Summary: The study developed a system for monitoring HIV-1 fate and showed its diverse applications in different environments. Transcriptome analysis of primary CD4+ T cells supporting alternative HIV-1 fates identified differential gene expression signatures. The research indicates that intra- and extra-cellular environments play a role in determining the fate of HIV-1 infection.
Article
Biology
Kyota Ishibashi, Kentaro Ikegami, Takashi Shimbo, Eiji Sasaki, Tomomi Kitayama, Yuzuru Nakamura, Takahiro Tsushima, Yasuyuki Ishibashi, Katsuto Tamai
Summary: This study demonstrates the cell heterogeneity in ACL and MCL ligaments using single-cell analysis. Three types of fibroblasts expressing Col14a1, Col12a1, or Col22a1 are identified. The ligaments show differences in cell composition and gene expression, which also change with age. This research provides a foundation for identifying potential therapeutic targets for ligament injuries.
COMMUNICATIONS BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yuan Wang, Qiaowen Zhao, Sen Liang, Mingliang Mei, Guangwei She, Wensheng Shi, Lixuan Mu
Summary: A silicon nanowire-based fluorescence lifetime thermometer (NWFLT) was developed for measuring intra- and extra-cellular temperatures simultaneously. The NWFLT revealed significant temperature heterogeneity along the longitudinal direction, particularly between the inside and outside of the cell.
CHEMICAL COMMUNICATIONS
(2023)
Article
Nutrition & Dietetics
Yui Nakayama, Yosuke Yamada, Shingo Ishii, Mai Hitaka, Keisuke Yamazaki, Motoyuki Masai, Nobuhiko Joki, Ken Sakai, Yasushi Ohashi
Summary: In this study involving 368 patients undergoing maintenance dialysis, a higher extracellular to intracellular water (ECW/ICW) ratio was associated with higher levels of natriuretic peptides and left ventricular mass index. The ECW/ICW ratio increased with decreasing intracellular water but not with extracellular water. This imbalance in ICW-ECW volume regulated by decreased cell mass may contribute to fluid accumulation in patients undergoing hemodialysis.
Review
Biochemistry & Molecular Biology
Siti A. M. Imran, Muhammad Dain Yazid, Wei Cui, Yogeswaran Lokanathan
Summary: TRF2 plays a crucial role in protecting telomeres and preventing DNA instability and diseases like cancer. Despite its ability to inhibit DNA damage response factors, more research is needed to understand its interactions outside of telomeres.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Matthias M. Fischer, Nils Bluthgen
Summary: This study investigates the effects of cellular dedifferentiation on tumor growth and treatment, and shows that dedifferentiation increases tumorigenesis and tumor growth speed. It also demonstrates that dedifferentiation may lead to treatment evasion, while targeting both cancer stem cells (CSCs) and non-stem cancer cells (NSCCs) in parallel is more effective in combating dedifferentiation.
JOURNAL OF THEORETICAL BIOLOGY
(2023)
Article
Medicine, General & Internal
Artur Mezheyeuski, Max Backman, Johanna Mattsson, Alfonso Martin-Bernabe, Chatarina Larsson, Ina Hrynchyk, Klara Hammarstrom, Simon Strom, Joakim Ekstrom, Siarhei Mauchanski, Salome Khelashvili, Amanda Lindberg, Margret Agnarsdottir, Per-Henrik Edqvist, Jutta Huvila, Ulrika Segersten, Per-Uno Malmstrom, Johan Botling, Bjoern Nodin, Charlotta Hedner, David Borg, Jenny Brandstedt, Hanna Sartor, Karin Leandersson, Bengt Glimelius, Anna Portyanko, Fredrik Ponten, Karin Jirstrom, Patrick Micke, Tobias Sjoblom
Summary: We evaluated 15 immune cell classes in 1481 tumor samples and generated an immune activation signature (SIA) based on the prognostic information of CD8+ lymphocytes and CD68+CD163+ macrophages in various cancer types. The SIA was associated with patient survival and had predictive value for response to checkpoint inhibitor therapy.
Article
Anesthesiology
Wanpei Chen, Ziwei Qi, Peng Fan, Naidong Zhang, Long Qian, Cui Chen, Yuhui Huang, Sanqing Jin
Summary: Dexmedetomidine, a commonly used anaesthetic adjuvant, does not affect the growth or metastasis of most tumors, but it inhibits the growth of colorectal cancer by modulating tumor CD4+ and CD8+ T cells.
BRITISH JOURNAL OF ANAESTHESIA
(2023)