Reverse genetics techniques: engineering loss and gain of gene function in plants

Detection of genomic deletions in rice using oligonucleotide microarrays

(2009) Myron Bruce et al.   BMC GENOMICS

A protocol for efficiently retrieving and characterizing flanking sequence tags (FSTs) in Brachypodium distachyon T-DNA insertional mutants

(2009) Vera Thole et al.   Nature Protocols

A multisite gateway-based toolkit for targeted gene expression and hairpin RNA silencing in tomato fruits

(2009) Leandro Hueso Estornell et al.   PLANT BIOTECHNOLOGY JOURNAL

Deletion-Based Reverse Genetics in Medicago truncatula

(2009) C. Rogers et al.   PLANT PHYSIOLOGY

Virus-induced gene silencing: A versatile tool for discovery of gene functions in plants

(2009) Arunima Purkayastha et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

High-Throughput Detection of Induced Mutations and Natural Variation Using KeyPoint™ Technology

(2009) Diana Rigola et al.   PLoS One

Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes

(2009) Aki Igarashi et al.   VIROLOGY

Evaluation of next generation sequencing platforms for population targeted sequencing studies

(2009) Olivier Harismendy et al.   GENOME BIOLOGY

Characterization of hemizygous deletions in Citrus using array-Comparative Genomic Hybridization and microsynteny comparisons with the poplar genome

(2008) Gabino Ríos et al.   BMC GENOMICS

Mutation detection using ENDO1: Application to disease diagnostics in humans and TILLING and Eco-TILLING in plants

(2008) Karine Triques et al.   BMC MOLECULAR BIOLOGY

Application of TILLING and EcoTILLING as Reverse Genetic Approaches to Elucidate the Function of Genes in Plants and Animals

(2008) N. Barkley et al.   CURRENT GENOMICS

Beyond silencing—engineering applications of RNA interference and antisense technology for altering cellular phenotype

(2008) Colin G Hebert et al.   CURRENT OPINION IN BIOTECHNOLOGY

Next-generation DNA sequencing

(2008) Jay Shendure et al.   NATURE BIOTECHNOLOGY

Gene silencing in plants using artificial microRNAs and other small RNAs

(2008) Stephan Ossowski et al.   PLANT JOURNAL

RNA Silencing in Plants: Yesterday, Today, and Tomorrow

(2008) A. Eamens et al.   PLANT PHYSIOLOGY

Establishment of a soybean (Glycine max Merr. L) transposon-based mutagenesis repository

(2008) Melanie Mathieu et al.   PLANTA