Article
Medicine, General & Internal
Janghee Lee, Young-jin Lee, Soong June Bae, Seung Ho Baek, Yoowon Kook, Yoon Jin Cha, Jong Won Lee, Byung Ho Son, Sei Hyun Ahn, Hee Jin Lee, Gyungyub Gong, Joon Jeong, Sae Byul Lee, Sung Gwe Ahn
Summary: This study evaluated the association between RS and Ki-67 expression, as well as the association between Ki-67 expression and recurrence-free survival, in patients with ER+/ERBB2- breast cancer. The findings demonstrate that high Ki-67 level is associated with increased risk of secondary endocrine resistance in patients with low genomic risk.
Article
Oncology
Rokshana Stephny Geread, Abishika Sivanandarajah, Emily Rita Brouwer, Geoffrey A. Wood, Dimitrios Androutsos, Hala Faragalla, April Khademi
Summary: This study proposes a novel proliferation index (PI) calculator for Ki67 images called piNET, which was successfully tested on multiple datasets, showing consistent performance and high accuracy using deep learning technology. Several innovations, such as adapting to wide variability of medical images, training purely on tumor cells, weak supervision on learning background regions, and introducing hotspot analysis for automated scoring, contribute to the system's success in improving accuracy and efficiency.
Article
Oncology
Susanna Thomas, Masrura Kabir, Belinda E. Butcher, Shaun Chou, Hema Mahajan, Gelareh Farshid, Rosemary Balleine, Nirmala Pathmanathan
Summary: The study aimed to assess the interobserver concordance of two methods for measuring Ki67 in breast cancer, showing that hot spot cell counting had excellent agreement among observers, while visual estimation performed best for high proliferation areas. Both methods demonstrated excellent prognostic agreement.
BREAST CANCER RESEARCH AND TREATMENT
(2021)
Article
Oncology
Yinxi Wang, Kimmo Kartasalo, Philippe Weitz, Balazs Acs, Masi Valkonen, Christer Larsson, Pekka Ruusuvuori, Johan Hartman, Mattias Rantalainen
Summary: The study demonstrated that deep learning analysis of transcriptome-wide expression morphology enables the prediction of mRNA expression and proliferation markers from routine histopathology whole slide images in breast cancer. This approach represents a significant advancement in predicting tumor molecular characteristics.
Article
Oncology
Mallikarjuna Siraganahalli Eshwaraiah, Aparna Gunda, Govind Babu Kanakasetty, Manjiri M. Bakre
Summary: This study compared the prognostic performance of CanAssist Breast (CAB) with Ki67 and found that CAB provided superior prognostic information, especially in the intermediate Ki67 group.
Article
Oncology
Amelie de Gregorio, Thomas Wolfram Paul Friedl, Eva Hering, Peter Widschwendter, Nikolaus de Gregorio, Inga Bekes, Wolfgang Janni, Davut Dayan, Jens Bodo Huober
Summary: This study retrospectively analyzed the Ki67 proliferative marker in breast cancer patients and its associations with clinicopathological factors, revealing that younger age, positive nodal status, higher grading, and positive HER2 status were significantly associated with higher Ki67 values.
Article
Cell Biology
Tatjana Vlajnic, Patrik Brunner, Serenella Eppenberger-Castori, Cyrill A. Rentsch, Tobias Zellweger, Lukas Bubendorf
Summary: The study found significant inter- and intratumoral variability of Ki67 LI in high-grade PC, with a subset of cases having surprisingly low Ki67 LI. There was no association observed between Ki67 LI and the status of ERG, PTEN, or Bcl-2. Further research is needed to explore the molecular basis and potential clinical implications of the paradoxically low proliferation rate in high-grade PC.
Article
Multidisciplinary Sciences
Farzin Negahbani, Rasool Sabzi, Bita Pakniyat Jahromi, Dena Firouzabadi, Fateme Movahedi, Mahsa Kohandel Shirazi, Shayan Majidi, Amirreza Dehghanian
Summary: The study highlights the importance of Ki-67 index and TILs in predicting tumor progression and chemotherapy response, while emphasizing the potential of deep learning methods for automated cell detection. The proposed dataset and method for estimating Ki-67 expression and TILs score in breast cancer cells show promising results compared to existing state-of-the-art methods.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Maha S. Al-Keilani, Rana I. Elstaty, Mohammad A. Alqudah, Asem M. Alkhateeb
Summary: Substance P is overexpressed in most breast cancer tissues and is significantly associated with breast cancer molecular subtypes, TNM stage, lymph node metastasis, ER and PR statuses. Ki-67 expression level also varies significantly with molecular subtypes, grade, lymph node metastasis, ER and PR statuses.
Article
Oncology
Rupa Guha, Binbin Yue, Jianping Dong, Aditi Banerjee, Ginette Serrero
Summary: Inhibiting PGRN/GP88 expression and blocking its action with AG01 antibody treatment can reduce proliferation and migration of TNBC cells, offering potential novel-targeted solutions in TNBC treatment.
BREAST CANCER RESEARCH AND TREATMENT
(2021)
Article
Chemistry, Multidisciplinary
Swapnali Londhe, Shagufta Haque, Sanchita Tripathy, Sreedhar Bojja, Chitta Ranjan Patra
Summary: The advancement of nanotechnology has led to the experimental development of silver nitroprusside nanoparticles (AgNNPs) for breast cancer therapy, which showed positive results in in vitro and in vivo experiments. AgNNPs exhibited biocompatibility with normal cell lines and cytotoxicity against cancer cell lines. They also demonstrated anti-angiogenic behavior and inhibited orthotopic breast tumor growth in mice. The possible molecular mechanisms for their anti-cancer activity were investigated. AgNNPs have the potential to be used as a generalized nanomedicine for breast and other cancers after proper biosafety evaluation in the future.
Article
Oncology
E. C. de Heer, C. E. Zois, E. Bridges, B. van der Vegt, H. Sheldon, W. A. Veldman, M. C. Zwager, T. van der Sluis, S. Haider, T. Morita, O. Baba, C. P. Schroder, S. de Jong, A. L. Harris, M. Jalving
Summary: The study found that the gene GYS1 is related to tumor growth and therapy resistance in breast cancer, especially in triple-negative breast cancer. Inhibiting GYS1 weakens the proliferation ability of breast cancer cells and makes them more sensitive to inhibition of mitochondrial proteostasis.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Cell Biology
Ayat Lashen, Michael S. Toss, Andrew R. Green, Nigel P. Mongan, Emad Rakha
Summary: This study compared several methods for scoring Ki67 in breast cancer, and found that quantifying Ki67-positive cells among 1000 tumor cells had the highest consistency between observers and was the most predictive of patient outcomes. The granular pattern of Ki67 expression was associated with poorer outcomes compared to other patterns.
Article
Oncology
Ulrike A. Nitz, Oleg Gluz, Sherko Kuemmel, Matthias Christgen, Michael Braun, Bahriye Aktas, Kerstin Luedtke-Heckenkamp, Helmut Forstbauer, Eva-Maria Grischke, Claudia Schumacher, Maren Darsow, Katja Krauss, Benno Nuding, Marc Thill, Jochem Potenberg, Christoph Uleer, Mathias Warm, Hans Holger Fischer, Wolfram Malter, Michael Hauptmann, Ronald E. Kates, Monika Graeser, Rachel Wuerstlein, Steven Shak, Frederick Baehner, Hans H. Kreipe, Nadia Harbeck
Summary: The WSG-ADAPT-HR+/HER2- study demonstrates the feasibility of guiding systemic treatment for breast cancer using both the recurrence score (RS) and endocrine therapy (ET) response, and avoids the use of chemotherapy in pre- and postmenopausal patients with <= 3 involved lymph nodes.
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Hui-Ju Chang, Ueng-Cheng Yang, Mei-Yu Lai, Chen-Hsin Chen, Yang-Cheng Fann
Summary: This study found that high BRCA1 gene expression is associated with increased tumour aggressiveness and risk of distant metastasis in breast cancers. High expression of proliferation marker genes was also found to be associated with high BRCA1 expression. The association between high BRCA1 expression and risk of distant metastasis was observed regardless of tamoxifen treatment.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Enrico Berrino, Laura Annaratone, Paolo Detillo, Dora Grassini, Alberto Bragoni, Anna Sapino, Benedetta Bussolati, Giovanni Bussolati, Caterina Marchio
Summary: Removing formic acid can significantly improve DNA preservation. ADF fixation reduces DNA fragmentation.
Review
Cell Biology
Maria P. P. Foschini, Rieko Nishimura, Viscardo Paolo Fabbri, Zsuzsanna Varga, Handan Kaya, Gabor Cserni
Summary: Myoepithelial cells (MECs) form a continuous layer surrounding the breast glands, acting as a barrier during neoplastic transformation. They can undergo various changes and give rise to a wide spectrum of morphological pictures seen in benign and malignant breast tumors. This study aims to provide a review of MECs lesions and their correlation to prognosis, as these tumors are relatively rare.
Article
Biochemistry & Molecular Biology
Iva Brcic, Andrea Maria Kluba, Theresa Marie Godschachner, Christoph Suppan, Peter Regitnig, Nadia Dandachi, Sigurd Friedwald Lax, Marija Balic
Summary: Male breast cancer (MBC) is rare and usually presents as a locally advanced disease. Stromal tumor-infiltrating lymphocytes (sTILs) are associated with a better response to neoadjuvant chemotherapy and improved prognosis in female breast cancer, but their role in MBC is less clear. This study examined the expression of sTILs, PD-L1, and pan-TRK in MBC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pathology
Enrico Berrino, Maria Costanza Aquilano, Emanuele Valtorta, Vito Amodio, Giovanni Germano, Marco Gusmini, Katiuscia Gizzi, Elisabetta Fenocchio, Anna Sapino, Silvia Marsoni, Andrea Sartore-Bianchi, Alberto Bardelli, Salvatore Siena, Emanuela Bonoldi, Caterina Marchio
Summary: Colorectal cancer cells often exhibit homogeneous retention or loss of mismatch repair (MMR) protein expression, but rare cases show heterogeneous patterns. In this study, 200 colorectal cancers were analyzed for MMR protein expression, resulting in three groups: proficient MMR, deficient MMR, and heterogeneous MMR expression. Heterogeneous MMR tumors were further examined and compared to the other groups, revealing differential gene expression patterns and increased tumor-infiltrating lymphocytes.
Correction
Pathology
Alberto Pisacane, Eliano Cascardi, Enrico Berrino, Alessio Polidori, Ivana Sarotto, Laura Casorzo, Mara Panero, Carla Boccaccio, Federica Verginelli, Silvia Benvenuti, Miriam Dellino, Paolo Comoglio, Filippo Montemurro, Elena Geuna, Caterina Marchio, Anna Sapino
Article
Pathology
Szintia Almasi, Levente Kuthi, Anita Sejben, Andras Voros, Akos Nagy, Tamas Zombori, Gabor Cserni
Summary: This study evaluated TRPS1 protein as a breast marker in cytokeratin-5-expressing TNBCs and found that TRPS1 was the most sensitive marker for the mammary origin of these tumors. TRPS1 positivity was higher than previously tested markers, and cases that were TRPS1-negative often expressed SOX10 or other markers. The evaluation demonstrated substantial agreement.
Review
Oncology
Andrea Ambrosini-Spaltro, Francesco Di Donato, Luca Saragoni, Gabor Cserni, Emad Rakha, Maria Pia Foschini
Summary: This study aimed to examine the prognostic factors of microinvasive breast carcinoma through a systematic review and meta-analysis. The results showed that lymph node status has a significant association with prognosis, while other factors such as estrogen receptor, progesterone receptor, HER2 status, multifocality or grade of microinvasion, and patient's age do not significantly affect prognosis. Therefore, lymph node status is the main prognostic factor of microinvasive breast carcinoma.
Article
Oncology
Balint Cserni, Darren Kilmartin, Mark O'Loughlin, Xavier Andreu, Zsuzsanna Bago-Horvath, Simonetta Bianchi, Ewa Chmielik, Paulo Figueiredo, Giuseppe Floris, Maria Pia Foschini, Aniko Kovacs, Paivi Heikkila, Janina Kulka, Anne-Vibeke Laenkholm, Inta Liepniece-Karele, Caterina Marchio, Elena Provenzano, Peter Regitnig, Angelika Reiner, Ales Ryska, Anna Sapino, Elisabeth Specht Stovgaard, Cecily Quinn, Vasiliki Zolota, Mark Webber, Sharon A. Glynn, Rita Bori, Erika Csorgo, Orsolya Olah-Nemeth, Tamas Pancsa, Anita Sejben, Istvan Sejben, Andras Voros, Tamas Zombori, Tibor Nyari, Grace Callagy, Gabor Cserni
Summary: Tumour-infiltrating lymphocytes (TILs) reflect the host's response against tumours, especially in triple-negative breast cancers. ONEST helps in estimating the number of observers required for a more reliable estimation of inter-observer agreement in TIL assessments, which has not been explored previously. This analysis suggests that between six and nine pathologists can provide a good approximation of inter-observer agreement in TIL assessments.
Review
Oncology
Ko Un Park, Chihwan Cha, Giada Pozzi, Young-Joon Kang, Vanesa Gregorc, Anna Sapino, Guglielmo Gazzetta, Emilia Marrazzo, Antonio Toesca
Summary: The review aims to summarize recent technical advancements in RNSM, discuss ongoing teaching programs, and address ongoing controversies.
CURRENT BREAST CANCER REPORTS
(2023)
Review
Oncology
Esther Diana Rossi, Zubair Baloch, Guliz Barkan, Maria Pia Foschini, Daniel Kurtycz, Marc Pusztaszeri, Philippe Vielh, William C. Faquin
Summary: The use of standardized reporting systems for non-gynecologic cytopathology, including the Milan System for Reporting Salivary Gland Cytopathology (MSRSGC), has gained popularity in the past decade. The MSRSGC defines diagnostic categories and provides risk assessment and management algorithms for salivary gland lesions. The second edition of MSRSGC, published in 2023, includes refined risks of malignancy, updates in ancillary testing, and a guide to the practical application of the latest ancillary markers.
CANCER CYTOPATHOLOGY
(2023)
Review
Cell Biology
Esther Diana Rossi, Zubair Baloch, Guliz Barkan, Maria Pia Foschini, Daniel Kurtycz, Marc Pusztaszeri, Philippe Vielh, William C. Faquin
Summary: The use of standardised reporting systems for non-gynaecologic cytopathology has gained popularity in various areas, including thyroid fine-needle aspiration and urine cytology. The Milan System for Reporting Salivary Gland Cytopathology (MSRSGC) provides a defined risk assessment and clinical management algorithm for diagnosing salivary gland lesions. The MSRSGC has been widely accepted and its second edition includes updated risk assessments and advancements in imaging and ancillary testing.
