Article
Energy & Fuels
Daniela Rodriguez-Luna, Hector A. Ruiz, Susana Gonzalez-Morales, Alberto Sandoval-Rangel, Marcelino Cabrera de la Fuente, Ana Charles-Rodriguez, Armando Robledo-Olivo
Summary: Lignocellulosic biomass is considered a promising raw material for ethanol production due to its low cost and availability. This study used melon waste as an inducer source to produce lignocellulosic enzymes through submerged fermentation. The melon waste showed high sugar content and significant amounts of hemicellulose, lignin, and cellulose. The Aspergillus niger M4 strain was capable of growing and synthesizing lignocellulose-degrading enzymes, with glucose concentration reaching 3.7 g/L. The culture broth exhibited significant endo-glucanase, xylanase, and laccase activities. The entire melon waste can be used as a substrate for lignocellulosic enzyme production, contributing to the biomass pretreatment for bioethanol production.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Forestry
Malik Owais Ullah Awan, Akhtar Iqbal, Muhammad Imtiaz Rashid, Usman Irshad, Farhan Hafeez, Farid Ullah, Muhammad Irshad, Gabrijel Ondrasek, Ivan Mustac, Rashid Nazir
Summary: The diversified Galiyat forests in the Lower Himalayas possess a potential source of lignocellulose degrading bacteria, with cellulose degraders, xylanase producers, and laccase producers identified among the isolates. These bacteria showed diversity in terms of their taxonomic classification and their ability to degrade different lignocellulosic components.
Article
Microbiology
Maria J. Lopez, Macarena M. Jurado, Juan A. . Lopez-Gonzalez, Maria J. Estrella-Gonzalez, Maria R. Martinez-Gallardo, Ana Toribio, Francisca Suarez-Estrella
Summary: This study investigated the dynamics, enzymes, and thermotolerance of lignocellulose-degrading thermophilic microbial community during plant waste composting, revealing the key role of thermophilic microorganisms in the breakdown of hemicellulose and the degradation of cellulose and lignin.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Applied
Liwei Gao, Guodong Liu, Qinqin Zhao, Ziyang Xiao, Wan Sun, Xianwei Hao, Xinmin Liu, Zhongfeng Zhang, Peng Zhang
Summary: This study expressed and screened three representative pectinolytic enzymes, and found that pectin methylesterase showed the strongest effect on the hydrolysis of beetroot residue, while maintaining the emulsifying activity of pectin. These findings provide the basis for optimizing the utilization of pectin-rich agricultural residues.
CARBOHYDRATE POLYMERS
(2022)
Article
Biochemistry & Molecular Biology
J. Ashwini John, Ethiraj Selvarajan
Summary: This study discusses the conversion of lignocellulosic waste to biofuel using cellulose in rice husks by lichen-associated Streptomyces sp. MS2A. The genome analysis of Streptomyces sp. MS2A confirmed the presence of cellulose-degrading genes and revealed the similarity with Streptomyces sp. CCM_MD2014. The analysis also identified a variety of GH families involved in lignocellulose degradation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Nicole Ing, Kai Deng, Yan Chen, Martina Aulitto, Jennifer W. Gin, Thanh Le Mai Pham, Christopher J. Petzold, Steve W. Singer, Benjamin Bowen, Kenneth L. Sale, Blake A. Simmons, Anup K. Singh, Paul D. Adams, Trent R. Northen
Summary: This study integrated two nanostructure-initiator mass spectrometry-based assays into a single multiplexed assay for quickly detecting glycan and lignin deconstruction enzymes, providing a tool for characterizing crude commercial enzyme mixtures. By applying this method, mixtures of enzymes suitable for biomass pretreatment can be discovered.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Chemical
Sandra Montoya, Alejandra Patino, Oscar J. Sanchez
Summary: Solid-state fermentation with Trametes versicolor in a fixed-bed bioreactor was studied with different aeration regimes. Results showed that low level of forced aeration favored laccase production and lignin degradation, highlighting the importance of aeration regime in enzyme production.
Article
Environmental Sciences
Anastasia Zerva, Romanos Siaperas, George Taxeidis, Maria Kyriakidi, Stamatina Vouyiouka, Georgios I. Zervakis, Evangelos Topakas
Summary: White-rot fungus Abortiporus biennis has been studied for its lignocellulolytic potential. The strain was found to possess all necessary enzymatic activities for complete biomass degradation, with oxidative enzymes playing a prominent role. Two novel laccases, AbiLac1 and AbiLac2, were discovered and shown to have the ability to oxidize a wide range of substrates. This study highlights the biotechnological potential of unexplored enzymatic machinery in white-rot basidiomycetes, including improved lignocellulolytic cocktails and the degradation/valorization of plastic waste materials.
Review
Materials Science, Paper & Wood
Shan Wei, Kaiquan Liu, Xingxiang Ji, Tengfei Wang, Ruiming Wang
Summary: Due to the rapid development of biotechnology, enzymes are being used more frequently in the manufacturing industry, especially in the pulp and paper industry. Biopulping offers advantages of mild reaction conditions and eco-friendliness, but to ensure maximum efficiency and quality, enzymatic methods often need to be combined with other pulping technologies. The cost of enzyme production remains a challenge to be addressed in future research.
Article
Agriculture, Dairy & Animal Science
Bruna C. Agustinho, Joao L. P. Daniel, Lucia M. Zeoula, Luiz F. Ferraretto, Hugo F. Monteiro, Matheus R. Pupo, Lucas G. Ghizzi, Mariele C. N. Agarussi, Celso Heinzen, Richard R. Lobo, Anay D. Ravelo, Antonio P. Faciola
Summary: The study found that the enzymatic complex from Pleurotus ostreatus had the highest activity at pH 5, with manganese peroxidase and LiP having the greatest activity at pH 4. The application rates of the enzymatic complex did not affect the fermentation profile and chemical composition at the onset of fermentation, but resulted in a linear decrease in lignin concentration of WPCS after 30 days of storage.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Qinqin Zhao, Zezheng Yang, Ziyang Xiao, Zheng Zhang, Jing Xing, Huiqi Liang, Liwei Gao, Jian Zhao, Yinbo Qu, Guodong Liu
Summary: This study identified alanine 873 as a potential target for engineering XYR1 to improve lignocellulolytic enzyme production in Trichoderma reesei. Mutation of this residue to tyrosine significantly enhanced the production of xylanolytic enzymes, especially when glucose was used as the carbon source.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Review
Chemistry, Physical
Giuseppe Olivieri, Rene H. Wijffels, Antonio Marzocchella, Maria Elena Russo
Summary: This review focuses on the development of enzymatic saccharification technologies in industrial processes, aiming to maximize sugar yields and concentrations, while minimizing the impact of enzyme costs. Discussions include bioreactor configurations, operating modes, and the application of laccase in lignin depolymerization.
Review
Engineering, Chemical
Attia Iram, Deniz Cekmecelioglu, Ali Demirci
Summary: This review paper summarizes key issues in using lignocellulosic biomass for energy production, including bottlenecks in enzyme production, ideal feedstock selection, and fermentation enhancement strategies.
Article
Biochemistry & Molecular Biology
Jinjing Zhang, Xinyi Zhuo, Qian Wang, Hao Ji, Hui Chen, Haibo Hao
Summary: This study investigated the effects of different nitrogen levels on carbon metabolism in Stropharia rugosoannulata using transcriptome analysis. The results showed that high nitrogen levels could enhance carbon metabolism in this mushroom, with upregulated expression of genes associated with starch and sucrose metabolism, nitrogen metabolism, and the MAPK signaling pathway. These findings contribute to the understanding of lignocellulose bioconversion pathways and can potentially improve biodegradation efficiency in Basidiomycetes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agricultural Engineering
Boliang Gao, Yangshuai Ma, Yiwen Xiao, Ya Wang, Yinghao Pan, Du Zhu
Summary: The production of lignocellulolytic enzyme cocktail by Chaetomium globosum DX-THS3 was optimized and its saccharification efficiency on raw rice straw was evaluated. The results showed that the enzyme cocktail had high enzymatic activity and excellent saccharification performance.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Food Science & Technology
Emanuele Catarina da Silva Oliveira, Jose Maria Rodrigues da Luz, Marina Gomes de Castro, Paulo Roberto Filgueiras, Rogerio Carvalho Guarconi, Eustaquio Vinicius Ribeiro de Castro, Marliane de Cassia Soares da Silva, Lucas Louzada Pereira
Summary: This study aimed to evaluate the effects of planting altitude and fruit fermentation on the chemical and sensory quality of coffee using Nuclear Magnetic Resonance (NMR) and Linear Discriminant Analysis (LDA). The results showed that different altitudes and fermentation methods can lead to variations in the chemical and sensory quality of coffee.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Tatielle Pereira Silva, Fabiana Sarmento de Albuquerque, Alexsandra Nascimento Ferreira, Davida Maria Ribeiro Cardoso dos Santos, Thatiane Verissimo dos Santos, Simoni Margareti Plentz Meneghetti, Marcelo Franco, Jose Maria Rodrigues da Luz, Hugo Juarez Vieira Pereira
Summary: This study investigated the utilization of agroindustrial residues in Brazil and found that pretreatment significantly increased the production of fermentable sugars. The use of sulfuric acid pretreatment was recommended.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Diene France de Souza, Marliane de Cassia Soares da Silva, Tainara Camila de Souza, Gabriel Cipriano Rocha, Maria Catarina Megumi Kasuya, Monique Renon Eller
Summary: This article presents the latest discoveries in the production of selenium-enriched edible mushrooms and their potential as an alternative source of this mineral in human and animal diets. Selenized mushrooms have been shown to possess bioaccessible forms of selenium with antioxidant and anti-tumor activities, reducing blood cholesterol and glucose levels. However, the use of selenium-enriched substrates may impact yield and chemical composition, posing challenges to standardization and scale-up of production.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Food Science & Technology
Marinalva Maria Bratz Simmer, Marliane de Cassia Soares da Silva, Lucas Louzada Pereira, Tais Rizzo Moreira, Rogerio Carvalho Guarconi, Tomas Gomes Reis Veloso, Isabelli Moreira Reboli da Silva, Thaynara Lorenzoni Entringer, Maria Catarina Megumi Kasuya, Jose Maria Rodrigues da Luz, Aldemar Polonini Moreli, Emanuele Catarina da Silva Oliveira
Summary: This study explores how the microbial communities (bacteria and fungi) of the soil and fruits of coffee trees can change depending on the harvest season, and how this affects the chemical and sensory quality of the coffee. The results suggest that edaphoclimatic factors and microbial communities are associated with the final quality of the coffee beverage.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Larissa Marcia Anastacio, Marliane de Cassia Soares da Silva, Danieli Grancieri Debona, Tomas Gomes Reis Veloso, Thaynara Lorenzoni Entringer, Vilian Borchardt Bullergahn, Jose Maria Rodrigues da Luz, Aldemar Polonini Moreli, Maria Catarina Megumi, Lucas Louzada Pereira
Summary: This study aimed to investigate the effects of different roasting processes on the physical structure of coffee beans and its correlation with sensory attributes of the beverage. The results showed that roasting processes can cause morphological and physical changes in coffee beans, which in turn affect the taste and aroma of the coffee.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Eloisio de Oliveira Martins, Jose Maria Rodrigues da Luz, Emanuele Catarina da Silva Oliveira, Rogerio Carvalho Guarconi, Tais Rizzo Moreira, Aldemar Polonini Moreli, Evandro Andrade Siqueira, Marliane de Cassia Soares da Silva, Marcus Roberto Goes Ferreira Costa, Lucas Louzada Pereira
Summary: This study analyzed the sensory and chemical characteristics of coffee grown in three agroforestry systems (AFSs) and subjected to five different post-harvest processing methods. The results showed that coffee produced in the natural/wild AFS and syntropic AFS had higher sensory scores compared to the conventional AFS. Spontaneous fermentation in covered terrain resulted in higher overall scores than fermentation in sealed polyethylene containers, except in the case of the conventional AFS. The body attribute contributed to the sensory discrimination of wet and dry fermentation processes. Lipids, caffeine, carboxylic acids, and chlorogenic acids were responsible for the chemical differentiation of AFSs. Therefore, AFSs and fermentation processes have an impact on the chemical and sensory quality of coffee beans.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Article
Ecology
Isabelle Goncalves Oliveira de Prado, Tomas Gomes Reis Veloso, Jose Maria Rodrigues da Luz, Marliane de Cassia Soares da Silva, Danielle Goncalves Oliveira de Prado, William F. J. Parsons, Damase P. Khasa, Maria Catarina Megumi Kasuya
Summary: The collapse of Fundao Dam in Mariana-MG, Brazil resulted in the discharge of iron ore mining waste into the Doce River watershed, causing negative impacts on the landscape. This study analyzed the soil microbiota as bioindicators of ecosystem recovery in areas affected and unaffected by mining tailings in the years following the collapse. The results showed that affected sites had different soil chemical properties, total fungal species, and arbuscular mycorrhizal fungi (AMF) compared to unaffected sites, indicating the importance of post-disturbance analysis in evaluating ecosystem recovery.
RESTORATION ECOLOGY
(2023)
Article
Environmental Sciences
Davida Maria Ribeiro Cardoso dos Santos, Fabiana Albuquerque, Tatielle Pereira Silva, Alexsandra Nascimento Ferreira, Sonia Salgueiro Machado, Jose Maria Rodrigues da Luze, Hugo Juarez Vieira Pereira
Summary: Agribusiness is a major sector of the Brazilian economy, but the improper disposal of agro-industrial waste has caused social and environmental issues. However, this waste can be utilized for fungal growth and the production of valuable enzymes. This study successfully produced an endoglucanase from Pycnoporus sanguineus using solid-state fermentation of agro-industrial waste. The enzyme displayed promising properties for obtaining fermentable sugars from waste for second-generation ethanol production.
WASTE AND BIOMASS VALORIZATION
(2023)
Article
Biotechnology & Applied Microbiology
Leandro de Souza Lopes, Marliane de Cassia Soares da Silva, Juliana Soares da Silva, Jose Maria Rodrigues da Luz, Alessandra de Oliveira Faustino, Gabriel Cipriano Rocha, Leandro Licursi de Oliveira, Maria Catarina Megumi Kasuya
Summary: This study evaluated the bioavailability of lithium-enriched Pleurotus djamor mushrooms in pigs and their effects on oxidative stress. The results showed that these mushrooms are a more bioavailable source of lithium and have protective effects against oxidative stress in animal tissues.
Article
Biotechnology & Applied Microbiology
Jose Maria Rodrigues da Luz, Leandro de Souza Lopes, Marliane de Cassia Soares da Silva, Nicolly Alves Vieira, Wilton Soares Cardoso, Maria Catarina Megumi Kasuya
Summary: Agro-industrial residue and textile effluents have caused environmental damage. Using Lentinula edodes to produce enzymes for the degradation of textile dyes can reduce these damages.