Journal
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
Volume 28, Issue 4, Pages 597-604Publisher
BRAZILIAN SOC CHEMICAL ENG
DOI: 10.1590/S0104-66322011000400005
Keywords
Sorghum Grains; Enzymatic Hydrolysis; Ethanol; Fermentation; Saccharomyces cerevisiae
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Funding
- Brazilian Council for Research (CNPq)
- Rio de Janeiro State Foundation for Science and Technology (FAPERJ)
- Brazilian Petroleum Company (PETROBRAS)
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The production of ethanol from sorghum grains was investigated in the present work. Initially, starch enzymatic hydrolysis was investigated using commercial alpha-amylase and glucoamylase, considering particle size, solid: liquid ratio and enzyme load as variables. The hydrolysate, in the best conditions (73 U of alpha-amylase/g grain and 1150 U glucoamylase/g grain), contained glucose at a concentration of approximately 250 g/L, which was fermented to produce ethanol in a bioreactor in batch mode. Using an industrial strain of Saccharomyces cerevisiae, the maximum ethanol concentration produced was roughly 106 g.L-1 in 24 h of fermentation, resulting in a volumetric productivity of 4.4 g.L-1.h(-1) and a product yield based on the substrate consumed (0.499 g.g(-1)) close to the theoretical.
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