4.7 Article

Drug delivery with nanospherical supramolecular cell penetrating peptide-taxol conjugates containing a high drug loading

Journal

JOURNAL OF COLLOID AND INTERFACE SCIENCE
Volume 453, Issue -, Pages 15-20

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2015.04.028

Keywords

Self-assembly; Supramolecular nanostructures; Taxol; Drug delivery; Cell penetrating peptide; Self-assembling peptide; Doxorubicin; Cancer therapy

Funding

  1. NSFC [81301311, 81071731, 81372844]
  2. program for Changjiang Scholars and Innovative Research Team in University [IRT13023]
  3. Ph.D. Programs Foundation of Ministry of Education of China [20131202110002]
  4. Tianjin Medical University Cancer Institute and Hospital Foundation of Seed Project [1201]

Ask authors/readers for more resources

Hypothesis: Supramolecular nanostructures via small molecule self-assembly hold great promise for controlled delivery of hydrophobic anticancer drugs. Particularly, taxol has recently been discovered to possess excellent self-assembly property, which may provide new opportunities to develop a new class of functional supramolecular nanomaterials for drug delivery application. Experiments: A cell penetrating peptide (CPP)-taxol conjugate (Taxol-CPP) was designed and synthesized. The self-assembling property of Taxol-CPP was investigated and the resultant nanomaterials were well characterized. Subsequently, the cytotoxicity of the Taxol-CPP after self-assembly against HepG2 cancer cells was evaluated. Findings: It is found that the Taxol-CPP possesses a high drug loading of 26.4% in each molecule, which is able to self-assemble into supramolecular nanospheres. By taking advantages of the self-assembly ability of taxol, Taxol-CPP supramolecular nanospheres with a mean size of around 130 nm can be obtained, composed of only the functional peptide (CPP) and the drug (taxol). Furthermore, we have demonstrated that the Taxol-CPP nanospheres do not compromise the taxol's potency, which can also be utilized as the carriers for co-delivery of another anticancer drug (doxorubicin). (C) 2015 Elsevier Inc. All rights reserved.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Chemistry, Multidisciplinary

Spatiotemporal Control over Chemical Assembly in Living Cells by Integration of Acid-Catalyzed Hydrolysis and Enzymatic Reactions

Xuejiao Yang, Honglei Lu, Yinghua Tao, Laicheng Zhou, Huaimin Wang

Summary: The study presents an efficient and general platform for controlling the formation of assemblies in living cells, using a protective strategy to resist hydrolysis and enable selective unmasking in the lysosomes. Demonstrated in vitro and in living cells, this spatiotemporal control of chemical assembly may have applications in various fields including supramolecular chemistry, materials science, synthetic biology, and chemical biology.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

Review Engineering, Biomedical

In Situ Construction of Functional Assemblies in Living Cells for Cancer Therapy

Xuejiao Yang, Zeyuan Cao, Honglei Lu, Huaimin Wang

Summary: Peptide-based materials show great potential for biomedical applications, but manipulating peptide assemblies in living cells is still a challenge. Recent research has focused on using endogenous triggers to construct peptide assemblies in vivo, with a particular emphasis on advancements in cancer therapy.

ADVANCED HEALTHCARE MATERIALS (2021)

Article Chemistry, Multidisciplinary

Intracellular Condensates of Oligopeptide for Targeting Lysosome and Addressing Multiple Drug Resistance of Cancer

Jing Wang, Liangbo Hu, Hongyue Zhang, Yu Fang, Tingliang Wang, Huaimin Wang

Summary: Biomolecular condensates play a crucial role in controlling cellular functions, but constructing artificial biomolecular condensates remains challenging. This study presents a general approach to construct biomolecular condensates in lysosomes of living cells for cancer therapy, with potential applications in addressing multiple drug resistance.

ADVANCED MATERIALS (2022)

Article Biotechnology & Applied Microbiology

Controlling supramolecular filament chirality of hydrogel by co-assembly of enantiomeric aromatic peptides

Xuejiao Yang, Honglei Lu, Yinghua Tao, Hongyue Zhang, Huaimin Wang

Summary: This study reports a method to control the fiber alignment, chirality, and stiffness of aromatic peptide hydrogels through heterodimerization of enantiomeric mixtures. The relative ratio of L and D enantiomers can also regulate the adhesion and morphology of mammalian cells. This work provides a new strategy for controlling supramolecular chirality in materials science.

JOURNAL OF NANOBIOTECHNOLOGY (2022)

Review Pharmacology & Pharmacy

Molecular Engineering of Peptide-Drug Conjugates for Therapeutics

Yu Fang, Huaimin Wang

Summary: In recent years, hundreds of novel small molecular drugs have been studied in clinical trials for various treatments worldwide. However, less than 10% of these drugs are eventually used due to diverse problems. In order to improve efficacy and efficiency, research has focused on the creation of assemblies of peptide-drug conjugates (PDCs) which have shown potential for overcoming complications. This review summarizes the recent developments in the creation of self-assembled nanostructures of PDCs for cancer therapy and discusses the chemical and physical properties of these drugs and peptides, as well as their mechanisms of action and remaining challenges in this field.

PHARMACEUTICS (2022)

Article Multidisciplinary Sciences

Self-assembled peptides-modified flexible field-effect transistors for tyrosinase detection

Huihui Ren, Tengyan Xu, Kun Liang, Jiye Li, Yu Fang, Fanfan Li, Yitong Chen, Hongyue Zhang, Dingwei Li, Yingjie Tang, Yan Wang, Chunyan Song, Huaimin Wang, Bowen Zhu

Summary: Flexible biosensors capable of real-time, non-invasive monitoring of cancer biomarkers are of great interest. In this study, high-performance tyrosinase-sensing field-effect transistor-based biosensors were successfully developed, with ultra-low detection limit and good mechanical flexibility.

ISCIENCE (2022)

Article Chemistry, Multidisciplinary

Biomimetic Heterodimerization of Tetrapeptides to Generate Liquid Crystalline Hydrogel in A Two-Component System

Bihan Wu, Shuang Zhao, Xuejiao Yang, Laicheng Zhou, Yang Ma, Hongyue Zhang, Wenbin Li, Huaimin Wang

Summary: This work describes the rational design of tetrapeptides to drive the formation of liquid crystalline hydrogel through intermolecular interactions. It also reveals the mechanism of self-assembly and the formation of anisotropic liquid crystalline domains. The diameter of nanofibers can be controlled by varying the enantiomer of electrostatic pairs. Furthermore, the hydrogel properties can be easily controlled by incorporating a light-responsive group.

ACS NANO (2022)

Article Chemistry, Multidisciplinary

Controlling Intracellular Enzymatic Self-Assembly of Peptide by Host-Guest Complexation for Programming Cancer Cell Death

Xuejiao Yang, Bihan Wu, Jiong Zhou, Honglei Lu, Hongyue Zhang, Feihe Huang, Huaimin Wang

Summary: This study demonstrates that host-guest complexation can efficiently regulate enzymatic self-assembly, leading to controlled cancer cell death.

NANO LETTERS (2022)

Article Chemistry, Multidisciplinary

Triggered Self-Sorting of Peptides to Form Higher-Order Assemblies in a Living System

Xuejiao Yang, Honglei Lu, Bihan Wu, Huaimin Wang

Summary: This research reports a biocompatible strategy for constructing self-sorted assemblies of peptides in living cells and tumor-bearing mice. The results demonstrate the control of cancer cell migration and tumor penetration ability through the self-assembly of designed peptides. The formation of self-sorted materials can be further programmed by introducing different triggers.

ACS NANO (2022)

Article Chemistry, Multidisciplinary

Confinement of Assemblies of Peptides by Chemical Reactions in Living Cells

Xuejiao Yang, Honglei Lu, Nan Kong, Huaimin Wang

Summary: The self-assembly of peptides is important in various fields such as optics, catalysis, medicine, and disease treatment. Peptide-based materials have shown great potential for cancer therapy and disease imaging due to their biocompatibility, tunability, and ease of functionality. Peptides can self-assemble into nanostructures in vivo triggered by endogenous stimuli, allowing for chemical reactions and achieving desired biological functions in the tumor microenvironment. This concept introduces the use of endogenous triggers to construct functional nanostructures in vivo and their corresponding biological applications.

CHEMISTRY-A EUROPEAN JOURNAL (2023)

Article Materials Science, Multidisciplinary

Glucose sensing by field-effect transistors based on interfacial hydrogelation of self-assembled peptide

Tengyan Xu, Huihui Ren, Yu Fang, Kun Liang, Hongyue Zhang, Dingwei Li, Yitong Chen, Bowen Zhu, Huaimin Wang

Summary: This study presents the first example of a peptidic hydrogel gated bio-FET by surface assisted self-assembly for label-free and real-time monitoring of glucose. The boric acid-containing peptide forms a hydrogel layer on the surface of In2O3, which interacts with glucose reversibly and influences the semiconductor's conductivity. The results demonstrate the superiority of hydrogel gated bio-FET for real-time monitoring of glucose in complex environments and its potential as a wearable device. This work illustrates a simple and fundamental strategy for integrating peptidic hydrogel with bio-FET for the real-time detection of analytes.

APPLIED MATERIALS TODAY (2023)

Review Engineering, Biomedical

Leading-Edge Pulmonary Gene Therapy Approached by Barrier-Permeable Delivery System: A Concise Review on Peptide System

Dinghao Chen, Nan Kong, Huaimin Wang

Summary: The lack of effective treatments for pulmonary diseases is a global health burden. Direct local gene therapy is an alternative method for treating pulmonary diseases. Compared to conventional viral/nonviral systems, peptide-vector-mediated in vivo lung gene therapies have various benefits. However, clinical trials in this area are still in the early stages. The major obstacle to delivering gene cargoes to the lungs is the presence of various extracellular mucosal layers and intracellular membranes. This review highlights recent developments in peptide-based gene delivery systems and their applications, including the designing rules for barrier-permeable pulmonary gene delivery, the use of oligopeptides for local gene therapy in lung tissue, and alternative hybridizing systems of peptides with other materials.

ADVANCED NANOBIOMED RESEARCH (2022)

Article Chemistry, Multidisciplinary

Hierarchical Self-assembly of G-Quadruplexes Based Hydrogel Consisting of Guanine and Peptide Epitope

Hongyue Zhang, Xuejiao Yang, Juan Liang, Kaihua Chen, Huaimin Wang

Summary: Guanosine-based hydrogels have limitations due to the necessity of sugar in hydrogel formation. A novel G-quadruplexes-based hydrogel was developed by using guanine and peptide epitope in the presence of metal cations. Metal cations can control the gelation time, nanofiber diameter, anisotropic property, and mechanical property of the hydrogel. This work demonstrates a simple way to develop soft materials by modulating the properties of nucleopeptide hydrogel.

CHINESE JOURNAL OF CHEMISTRY (2023)

Article Chemistry, Multidisciplinary

Intramitochondrial co-assembly between ATP and nucleopeptides induces cancer cell apoptosis

Huyeon Choi, Gaeun Park, Eunhye Shin, Seon Woo Shin, Batakrishna Jana, Seongeon Jin, Sangpil Kim, Huaimin Wang, Sang Kyu Kwak, Bing Xu, Ja-Hyoung Ryu

Summary: Mitochondria-targeting nucleopeptide can sequester ATP by self-assembling, leading to apoptosis of cancer cells.

CHEMICAL SCIENCE (2022)

Article Chemistry, Multidisciplinary

Tandem molecular self-assembly for selective lung cancer therapy with an increase in efficiency by two orders of magnitude

Debin Zheng, Jingfei Liu, Yinghao Ding, Limin Xie, Yingying Zhang, Yaoxia Chen, Rong Peng, Miao Cai, Ling Wang, Huaimin Wang, Jie Gao, Zhimou Yang

Summary: The study successfully designed a tandem molecular self-assembling prodrug that significantly improved the therapeutic efficacy of HCPT against lung cancer. The prodrug utilized elevated levels of alkaline phosphatase and reductase in lung cancer cells for self-assembly, effectively preventing the disassembly and biodegradation of the nanofiber, and significantly enhancing the efficacy of HCPT against lung cancer.

NANOSCALE (2021)

Article Chemistry, Physical

Mechanistic exploration of Co doping in optimizing the electrochemical performance of 2H-MoS2/N-doped carbon anode for potassium-ion battery

Panpan Zhang, Xu Wang, Yangyang Yang, Haifeng Yang, Chunsheng Lu, Mingru Su, Yu Zhou, Aichun Dou, Xiaowei Li, Xiaochuan Hou, Yunjian Liu

Summary: In this study, the influence of transition metal doping on the electronic and mechanical properties and electrochemical performance of 2HMoS2/NC was investigated using Cobalt (Co) as an example. Co doping was found to effectively improve the electronic conductivity and active site areas of 2H-MoS2/NC at different positions, optimizing the adsorption and diffusion capability of potassium ions. Furthermore, the study revealed the optimal roles of different types of nitrogen atoms in kinetic adsorption, diffusion, and interfacial stability of potassium ions. These findings provide guidance for the experimental design of high rate 2H-MoS2/NC electrode materials and the optimal design of other functional composite materials.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024)

Article Chemistry, Physical

Addressing the synchronized impact of a novel strontium titanium over copolymerized carbon nitride for proficient solar-driven hydrogen evolution

Zeeshan Ajmal, Mahmood Ul Haq, Shahid Zaman, M. K. Al-Muhanna, Anuj Kumar, Mohammed M. Fadhali, Siwar Ben Hadj Hassine, Muhammas Qasim, K. F. Alshammari, Ghulam Abbas Ashraf, Abdul Qadeer, Adil Murtaza, Sulaiman Al-Sulaimi, Huaqiang Zeng

Summary: This study presents a novel heterojunction structure (SrTiO3/CN-TAL10.0) for enhanced photocatalytic water splitting (PWS). The incorporation of thiophenedicarboxaldehyde (TAL) through copolymerization significantly improves the photocatalytic activity of carbon nitride (CN) while maintaining its photostability performance. The optimized composition allows efficient isolation of photoinduced charge carriers and enhanced charge transport, resulting in a remarkable increase in overall photocatalytic efficiency.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024)

Article Chemistry, Physical

Magnetic-driven Interleukin-4 internalization promotes magnetic nanoparticle morphology and size-dependent macrophage polarization

Angela Arnosa-Prieto, Patricia Diaz-Rodriguez, Manuel A. Gonzalez-Gomez, Pelayo Garcia-Acevedo, Lisandra de Castro-Alves, Yolanda Pineiro, Jose Rivas

Summary: Macrophages can exhibit different phenotypes depending on the microenvironment and the characteristics of magnetic iron oxide nanoparticles (MNPs). This study demonstrates that the concentration and morphology of MNPs can influence the polarization of macrophages. The findings have implications for therapeutics targeting tissue regeneration and tumor progression.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024)

Article Chemistry, Physical

Polyamide nanofiltration membranes by vacuum-assisted interfacial polymerization: Broad universality of Substrate, wide window of monomer concentration and high reproducibility of performance

Yu Fang, Cheng-Ye Zhu, Hao-Cheng Yang, Chao Zhang, Zhi-Kang Xu

Summary: This study demonstrates the advantages of vacuum-assisted interfacial polymerization (VAIP) in fabricating polyimide nanofiltration membranes. By using vacuum filtration, aqueous solutions of PIP can be evenly distributed on different microfiltration substrates, leading to the fabrication of uniform and ultra-thin polyamide layers with excellent performance. The membranes exhibit high rejection rates and water permeance, as well as satisfactory long-term stability.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024)

Article Chemistry, Physical

Comparing polymer-surfactant complexes to polyelectrolytes

Isaac J. Gresham, Edwin C. Johnson, Hayden Robertson, Joshua D. Willott, Grant B. Webber, Erica J. Wanless, Andrew R. J. Nelson, Stuart W. Prescott

Summary: Understanding the interactions between polymers and surfactants is crucial for optimizing commercial systems. This study tested the behavior of polymer-surfactant systems, revealing that they do not behave like polyelectrolytes in the presence of salt. Additionally, the structure of polymer-surfactant complexes under confinement differs from that of polyelectrolytes.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024)

Article Chemistry, Physical

Crosslinking alginate at water-in-water Pickering emulsions interface to control the interface structure and enhance the stress resistance of the encapsulated probiotics

Yunxiao Xie, Cui Liu, Jie Zhang, Yan Li, Bin Li, Shilin Liu

Summary: This study aimed to improve the microstructure and rheological properties of W/W Pickering emulsions by crosslinking sodium alginate at the water-water interface, thereby enhancing the activity of encapsulated probiotics in simulated gastrointestinal digestion.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024)

Article Chemistry, Physical

Multi-layering of carbon conductivity enhancers for boosting rapid recharging performance of high mass loading lithium ion battery electrodes

Sang Ho Lee, Yige Sun, Patrick S. Grant

Summary: This research developed an effective approach to enhance the charging rates of lithium ion batteries (LIBs) by strategically incorporating carbon nanotube (CNT) conductivity boosters into Li4Ti5O12 (LTO) electrodes. Multi-layer architectures comprising CNT-rich and CNT-free LTO electrode layers were manufactured using a layer-by-layer spray coating method to promote charge transfer kinetics of high mass loading electrodes. The best performing multi-layer was paired with a spray-coated LiFePO4 (LFP) positive electrode, resulting in attractive power performance that outperformed conventional LTO || LFP combinations.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024)

Article Chemistry, Physical

Defect engineering induces Mo-regulated Co9Se8/FeNiSe heterostructures with selenium vacancy for enhanced electrocatalytic overall water splitting in alkaline

Jingwei Liang, Shaobin Li, Fengbo Li, Li Zhang, Yufeng Jiang, Huiyuan Ma, Kun Cheng, Liang Qing

Summary: A molybdenum-regulated self-supporting electrode material with rich vacancy defects has been successfully synthesized and shows exceptional catalytic activities and stability for electrocatalytic overall water splitting. This study provides a new perspective for the design and synthesis of non-precious metal bifunctional electrocatalysts.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024)

Article Chemistry, Physical

Surfactant-free microemulsions (SFMEs) as a template for porous polymer synthesis

Jonas Blahnik, Jennifer Schuster, Rainer Mueller, Eva Mueller, Werner Kunz

Summary: This study investigates the relationship between the morphology of PMMA monopolymers and PMMA-PHEMA copolymers with the expected nature of surfactant-free microemulsions (SFMEs) before polymerization. It is found that previously mesostructured, surfactant-free mixtures can produce porous polymers of different morphologies, while unstructured, oil-rich regions lead to solid, transparent polymers without nanostructured morphologies. Additionally, a surfactant-based reference system shows similar phase behavior and polymer morphologies as the comparable surfactant-free system, indicating the importance of the hydrotropic behavior of HEMA in this system.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024)

Article Chemistry, Physical

Morphology regulation of isomeric covalent organic frameworks for high selective light scattering detection of lead

Zheng-Fen Pu, Wen-Zhi She, Rong Sheng Li, Qiu-Lin Wen, Bi-Chao Wu, Chun-Hua Li, Jian Ling, Qiue Cao

Summary: This study synthesized two framework-isomeric covalent organic frameworks (COFs) and discovered that the light scattering signal of COFs can be used for the analytical detection of lead ions. By controlling synthesis conditions and introducing regulators, the morphology of COFs could be controlled and framework-isomeric COFs could be precisely synthesized.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024)

Article Chemistry, Physical

Preparation and dynamic color-changing study of fluorescent polymer nanoparticles for individualized and customized anti-counterfeiting application

Yuchen Weng, Ying Hong, Jingyu Deng, Sicheng Cao, Li-Juan Fan

Summary: This paper reports the preparation of dynamic color-changing fluorescent polymer nanoparticles (PNPs) by constructing a fluorescence resonance energy transfer (FRET) pair. The PNPs show excellent anti-counterfeiting effects and reproducibility. The study demonstrates a promising encryption strategy that can achieve multiple outputs with simple operation.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024)

Article Chemistry, Physical

Employing polyaniline/viologen complementarity to enhance coloration and charge dissipation in multicolor electrochromic display with wide modulation range

Guodong Liu, Zijian Wang, Jianing Wang, Hanbin Liu, Zhijian Li

Summary: This study investigates the combination of multicolor switchable polyaniline (PANI) electrode and 1-methyl-4,4'-bipyridyl iodide (MBI), which demonstrates superior optical properties in visible and near-infrared light modulation, as well as excellent electrochemical performances. This combination can be used to develop novel electrochromic devices for applications in smart packaging, smart labels, and flexible smart windows.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024)

Article Chemistry, Physical

Facile constructing Ti3C2Tx/TiO2@C heterostructures for excellent microwave absorption properties

Huying Yan, Yang Guo, Xingzhi Bai, Jiawei Qi, Haipeng Lu

Summary: By modifying Ti3C2Tx through heterogeneous interface engineering, optimized impedance matching is achieved, leading to enhanced electromagnetic wave absorption performance.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024)

Article Chemistry, Physical

Reversible crowdedness of pH-responsive and host-guest active polymersomes: Mimicking μm-sized cell structures

Kehu Zhang, Yang Zhou, Silvia Moreno, Simona Schwarz, Susanne Boye, Brigitte Voit, Dietmar Appelhans

Summary: This article presents an advanced crosslinking strategy to fabricate clustered polymersomes using host-guest interactions. By controlling the input of crosslinker and environmental conditions, reversible aggregation and disassembly of these polymersomes can be achieved. The size and structure of these clustered polymersomes can be regulated and visualized through a fluorescent enzymatic cascade reaction.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024)

Article Chemistry, Physical

Potassium regulating electronic state of zirconia supported palladium catalyst and hydrogen spillover for improved acetylene hydrogenation

Junjie Xu, Weixiong Huang, Ruiling Li, Li Li, Jinjin Ma, Jiaou Qi, Haiyan Ma, Min Ruan, Lilin Lu

Summary: In this study, a potassium doped palladium catalyst was developed for acetylene hydrogenation, showing excellent catalytic performance and durability. The doping of potassium effectively weakened the adsorption of ethylene, improved ethylene selectivity, and lowered the barriers of hydrogen activation and transfer reactions.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024)