How to perform RT-qPCR accurately in plant species? A case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping population
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Title
How to perform RT-qPCR accurately in plant species? A case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping population
Authors
Keywords
RT-qPCR, Flower colour, RNA quality, noRT, Standard curves, Reference genes, Gene expression, Pink
Journal
BMC MOLECULAR BIOLOGY
Volume 14, Issue 1, Pages 13
Publisher
Springer Nature
Online
2013-06-24
DOI
10.1186/1471-2199-14-13
References
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- (2012) J. V. Die et al. JOURNAL OF EXPERIMENTAL BOTANY
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- (2011) Xin Sui et al. PLANT CELL REPORTS
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- (2011) Yusuke Akita et al. PLANTA
- Genetic engineering of flavonoid pigments to modify flower color in floricultural plants
- (2010) Masahiro Nishihara et al. BIOTECHNOLOGY LETTERS
- Experimental comparison of relative RT-qPCR quantification approaches for gene expression studies in poplar
- (2010) Nicole Regier et al. BMC MOLECULAR BIOLOGY
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- Comparison of different standards for real-time PCR-based absolute quantification
- (2010) S. Dhanasekaran et al. JOURNAL OF IMMUNOLOGICAL METHODS
- Normalizing RT-qPCR Data: Are We Getting the Right Answers? An Appraisal of Normalization Approaches and Internal Reference Genes from a Case Study in the Finfish Lates calcarifer
- (2010) Christian De Santis et al. MARINE BIOTECHNOLOGY
- Natural and engineered nicking endonucleases--from cleavage mechanism to engineering of strand-specificity
- (2010) S.-H. Chan et al. NUCLEIC ACIDS RESEARCH
- Advances in Genetical Genomics of Plants
- (2009) R. Joosen et al. CURRENT GENOMICS
- Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references
- (2009) S. Guenin et al. JOURNAL OF EXPERIMENTAL BOTANY
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- The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants
- (2008) Laurent Gutierrez et al. PLANT BIOTECHNOLOGY JOURNAL
- Towards a Systematic Validation of References in Real-Time RT-PCR
- (2008) L. Gutierrez et al. PLANT CELL
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