Article
Multidisciplinary Sciences
Georgia Diakoudi, Paolo Capozza, Gianvito Lanave, Francesco Pellegrini, Barbara Di Martino, Gabriella Elia, Nicola Decaro, Michele Camero, Paola Ghergo, Fabio Stasi, Alessandra Cavalli, Maria Tempesta, Vanessa R. Barrs, Julia Beatty, Krisztian Banyai, Cristiana Catella, Maria S. Lucente, Alessio Buonavoglia, Giovanna Fusco, Vito Martella
Summary: Hepadnaviruses can be found in dogs, similar to the hepadnavirus in domestic cats. Canine hepadnavirus can cause altered hepatic markers, but its role in canine health remains unclear.
SCIENTIFIC REPORTS
(2022)
Review
Chemistry, Analytical
Lulu Xu, Jiaxin Duan, Junman Chen, Shijia Ding, Wei Cheng
Summary: RCA is an efficient enzymatic isothermal reaction using circular probes to amplify circular templates rapidly, with high specificity and the ability to generate customized functional nucleic acids for in situ detection.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Moon Hyeok Choi, Guralamatta Siddappa Ravi Kumara, Jaehyeon Lee, Young Jun Seo
Summary: In this paper, a molecular diagnostic system combining a colorimetric probe and isothermal gene amplification is reported for clinical point-of-care diagnosis of SARS-CoV-2. The system demonstrates high sensitivity and selectivity, with detection of SARS-CoV-2 within 1 hour and discrimination from other respiratory viral genomes. It offers a simple and rapid detection method that can be performed without instrumentation.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Gun-Soo Park, Jin-Soo Maeng
Summary: We developed a method for amplifying kilobases-long target sequence from RNA templates using Cre recombinase to generate circular intermediate template for subsequent amplification. The method can amplify a target sequence of about 2.9 kb, and the amplicon can be used for cloning.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Xiaowen Cao, Feng Chen, Jing Xue, Yue Zhao, Min Bai, Yongxi Zhao
Summary: Here, we present a hierarchical DNA branch assembly-encoded fluorescent nanoladders for denoised and highly multiplexed signal amplification of single-cell transcripts. Our method achieves independent RNA-primed rolling circle amplification without nonspecific amplification and allows for programmable DNA branch assembly to encode virtual signals for visualizing numbers of targets. We demonstrate accurate RNA profiling in different cancer cells and reveal diverse localization patterns for spatial regulation of transcripts.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Analytical
Yanfei Zhang, Jun Chen, Huihui Yang, Wen Yin, Chunrong Li, Yuzhi Xu, Si-Yang Liu, Zong Dai, Xiaoyong Zou
Summary: The LCR-HCR method uses exosomes as vehicles for controlled release and recruitment, significantly improving miRNA imaging contrast by confining reactants on exosome vehicles. It provides a powerful tool for sensitive miRNA imaging in living cells and cancer diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Jun Zhao, Jiandong Wu, Xiaosong Wu, Guoqing Deng, Yong Liu, Francis Lin, Ling Zhu
Summary: An efficient self-quenching fluorescence probe and the corresponding detection system were designed, capable of achieving a wide dynamic range and low detection limit in a single step. The method, with high specificity and biocompatibility, has great potential for accurately quantifying miRNA biomarkers in serum, providing promising prospects for clinical diagnosis.
CHEMICAL COMMUNICATIONS
(2021)
Review
Chemistry, Multidisciplinary
Chi Yao, Junhan Ou, Jianpu Tang, Dayong Yang
Summary: Facing the increasing demand for precision medicine, materials chemistry systems with accurate molecular design, controllable structure, and adjustable biological activity are required for bioanalysis. In recent years, DNA has been considered a potential biomaterial for analysis and has been applied in various fields. DNA supramolecular assembly on micro/nanointerfaces is an effective strategy to concentrate functional DNA modules and enhance their functions in bioanalysis. This research direction is expected to guide the construction of more bioanalytical materials and promote the development of precision medicine.
ACCOUNTS OF CHEMICAL RESEARCH
(2022)
Article
Chemistry, Analytical
Meng Chen, Qidi He, Yanli Tong, Zuanguang Chen
Summary: This study successfully combined LAMP with RCA to achieve rapid detection and quantification of pathogens. By using a strand displacement strategy in a single-tube reaction, different pathogen target genes were successfully detected, demonstrating the universality and reliability of the method.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Shu-An Hsieh, Danial Shamsaei, Derek R. Eitzmann, Jared L. Anderson
Summary: This study demonstrates the use of a 3D printed droplet generation platform coupled with molecular beacons in LAMP detection, allowing for low-cost and customizable droplet generation. The study also demonstrates the use of a smartphone-based imaging system to increase accessibility. This method can be used for sequence-specific, sensitive, and accurate DNA quantification.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Guang Li, Shengjie Ge, Ping Niu, Jianyou Zhang, Yu Mao, Youwei Wang, Aidong Sun
Summary: A high-throughput surface-enhanced Raman scattering-based lateral flow assay is proposed for accurate quantification of ctDNA associated with head and neck squamous cell carcinoma. The method combines high-performing SERS probes and target-specific signal amplification strategy, enabling fast and parallel detection of multiple samples.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Chemistry, Multidisciplinary
Jinhua Shang, Shanshan Yu, Ruomeng Li, Yuqiu He, Yushi Wang, Fuan Wang
Summary: Rolling circle amplification (RCA) is used to construct compact and versatile DNA nanoassemblies, but it is rarely used for intracellular analysis due to uncontrollable and inefficient probe integration/activation. Here, we developed a multifunctional RCA nanogel encoded with tandem allosteric deoxyribozyme for efficient intracellular microRNA imaging. The endogenous microRNA stimulates the precise degradation of DNA nanocarriers, leading to the exposure of RCA-entrapped DNAzyme biocatalyst for amplified readout signal. Our bioorthogonal DNAzyme disassembly strategy enables robust analysis of intracellular biomolecules with potential applications in clinical diagnosis.
Article
Chemistry, Analytical
Chenxin Fang, Ping Ouyang, Yuxing Yang, Yang Qing, Jialun Han, Wenyan Shang, Yubing Chen, Jie Du
Summary: A microRNA detection platform utilizing a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system has been proposed for rapid and efficient detection of miRNA-21. This system achieves signal amplification and fluorescence intensity amplification through different mechanisms, showing broad prospects in nucleic acid analysis and detection.
Article
Chemistry, Analytical
Juan Song, Shan Li, Jie Zhou, Qiao Yu, Xue-Jiao Yang, Hong-Yuan Chen, Jing-Juan Xu
Summary: We have developed a triple signal amplification strategy for in situ visualization of telomerase RNA (TR) in living cells. The strategy includes target-initiated strand displacement circuit, multidirectional rolling circle amplification (RCA), and Mg2+ DNAzyme-mediated amplification. The proposed method exhibited ultrasensitive detection of TR mimic with a detection limit at the picomolar level. Moreover, after transfection with the proposed sensing platform, tumor cells can be easily distinguished from normal cells based on TR abundance-related fluorescence signal, providing new insights into initial cancer screening.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Yu Yan, Dingran Chang, Yongbin Xu, Yangyang Chang, Qiang Zhang, Quan Yuan, Bruno J. Salena, Yingfu Li, Meng Liu
Summary: Functional nucleic acids (FNAs) can be engineered into circular forms for improved performance. However, synthesizing circular single-stranded DNA molecules in high yield remains a challenge.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
