Article
Biochemistry & Molecular Biology
Harpreet Kaur, Vinay Modgil, Naveen Chaudhary, Balvinder Mohan, Neelam Taneja
Summary: Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) and their extended-spectrum beta-lactamase (ESBL) production pose a significant threat to current antimicrobial treatment options. To overcome this challenge, the identification of new therapeutic targets is crucial. In this study, we used computational tools to identify potential drug targets against the ESBL-producing UPEC strain NA114, focusing on chokepoint, virulence, and resistance genes. Through a hierarchical in silico technique, we discovered seven distinct targets for this pathogen that showed no similarity to the human proteome. These targets, involved in bacterial peptidoglycan biosynthesis and inositol phosphate metabolism, hold promise for the design of effective drugs against MDR UPEC.
Article
Microbiology
Rory Cave, Mary M. Ter-Stepanyan, Nune Kotsinyan, Hermine Mkrtchyan
Summary: We conducted whole-genome sequencing and analysis of ESBL UPEC ST127 isolates recovered from patients in hospitals in Armenia. Our findings indicate that E. coli ST127 was a dominant lineage causing urinary tract infections in different regions of Armenia. We also identified unique genes in Armenian and French isolates that were absent in other ST127 isolates obtained from the European Nucleotide Archive (ENA). These findings suggest a possible cross-country transmission and highlight the importance of implementing whole-genome sequencing surveillance in tackling antibiotic resistance.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Farah Al-Marzooq, Akela Ghazawi, Saeed Tariq, Lana Daoud, Timothy Collyns
Summary: This study investigated the synergistic effect of polymyxin B nonapeptide (PMBN) with azithromycin (AZT) in fighting multidrug-resistant (MDR) pathogens. Results showed that PMBN was able to re-sensitize bacteria to AZT, indicating that using multiple antimicrobial agents could be a promising strategy in eradicating MDR bacteria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Steev Loyola, Fatima Concha-Velasco, Jimena Pino-Duenas, Nancy Vasquez-Luna, Paola Juarez, Carlos Llanos, Guillermo Salvatierra, Jesus Tamariz, Andres G. Lescano
Summary: This study investigated the antimicrobial resistance and dynamics of ESBL-producing UPEC isolates from UTI cases seen at a local hospital in Cusco, Peru. The results showed high levels of multidrug resistance in ESBL-producing UPEC isolates, with varying resistance patterns over time and differences based on gender and healthcare service.
Article
Microbiology
Nina Langkabel, Janine Burgard, Sabrina Freter, Reinhard Fries, Diana Meemken, Luppo Ellerbroek
Summary: ESBL-producing E. coli is considered a major hazard in poultry, with poultry meat being a potential source of human infection. This study found ESBL-producing E. coli in broiler breast skin samples from different abattoirs, with the lowest incidence in the abattoir using conventional immersion scalding. Good kitchen hygiene is important to prevent infection by ESBL-producing E. coli and highly pathogenic phylogroups through chicken meat.
Article
Pharmacology & Pharmacy
Ting Huang, Zheng Lv, Jiafu Lin, Kelei Zhao, Longfei Zhai, Xinrong Wang, Yiwen Chu
Summary: This study reports the potent combination of linezolid and polymyxin B nonapeptide PBNP (LP) against Klebsiella pneumoniae (KP) infection in vitro and in vivo. LP combination showed synergistic antibacterial effects against KP and protected Caenorhabditis elegans from KP infection. The study suggests LP as a potential activity enhancer and alternative treatment for antibiotic resistance.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Medicine, General & Internal
Monica Romero Nieto, Sara Maestre Verdu, Vicente Gil, Carlos Perez Barba, Jose Antonio Quesada Rico, Reyes Pascual Perez
Summary: This study identified male sex, smoking, hypertension, urinary incontinence, and recurrent urinary tract infections as independent factors associated with the presence of ESBL-producing E. coli in patients with acute community-acquired pyelonephritis. The multivariate logistic models could correctly classify individuals with ESBL-producing E. coli infection in 80.2% of cases.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Infectious Diseases
Cailean Carter, Alexandra Hutchison, Steven Rudder, Elizabeth Trotter, Emma Waters, Ngozi Elumogo, Gemma C. Langridge
Summary: This study aimed to identify the clonal groups and resistance genes that are spreading in the community and hospitals in Norfolk. The main causative agent for UTIs in this region is uropathogenic Escherichia coli (UPEC), with significant multi-drug resistance observed in ST131 and ST1193 lineages. Monitoring samples and considering sources can help reduce the burden of disease.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Camille Chagneau, Clemence Massip, Nadege Bossuet-Greif, Christophe Fremez, Jean-Paul Motta, Ayaka Shima, Celine Besson, Pauline Le Faouder, Nicolas Cenac, Marie-Paule Roth, Helene Coppin, Maxime Fontanie, Patricia Martin, Jean-Philippe Nougayrede, Eric Oswald
Summary: This study found the presence of the genotoxin Colibactin in the urine of patients with urinary tract infections, and demonstrated its genotoxicity in bladder cells. This discovery raises the hypothesis of a potential link between urinary tract infections and bladder cancer.
Article
Infectious Diseases
Daniel Martak, Julia Guther, Tess D. Verschuuren, Benoit Valot, Nadine Conzelmann, Stefanie Bunk, M. Eugenia Riccio, Elena Salamanca, Alexandre Meunier, Charles P. Henriot, Caroline Pressacco Brossier, Xavier Bertrand, Ben S. Cooper, Stephan Harbarth, Evelina Tacconelli, Ad C. Fluit, Jesus Rodriguez-Bano, Jan A. J. W. Kluytmans, Silke Peter, Didier Hocquet
Summary: This study in five European cities found that food items were not the main source of ESBL-Ec and ESBL-Kp, as the strains from contaminated environments were genetically distinct from those in food. The findings suggest that human-to-human transmission is possibly the most frequent route of ESBL-Ec and ESBL-Kp transmission in high-income countries.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Review
Microbiology
Shweta R. Singh, Alvin Kuo Jing Teo, Kiesha Prem, Rick Twee-Hee Ong, Elizabeth A. Ashley, H. Rogier van Doorn, Direk Limmathurotsakul, Paul Turner, Li Yang Hsu
Summary: Despite limited knowledge on the prevalence of ESBL-E and CPE in the Greater Mekong Subregion, recent trends show an increase in ESBL-producing E. coli in clinical and carriage specimens. Risk factors associated with ESBL-E isolation include recent antibiotic exposure, chronic kidney disease, and other co-morbidities. Collaboration on standardizing study methods is needed to improve monitoring and surveillance in the region, especially in community settings of Laos, Myanmar, Cambodia, and Yunnan and Guangxi provinces of China.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Salwa Muhsin Hasan, Khalid S. Ibrahim
Summary: The presence of ESBL-producing bacteria in pediatric urinary tract infections is increasing worldwide. This study investigated the prevalence of E. coli infections, antimicrobial resistance patterns, and the presence of ESBL-producing E. coli and virulence factor genes in UTI patients among children in Duhok Province, Kurdistan, Iraq. The results showed a high proportion of UPEC infections in children under 5 years old, with significantly higher rates among girls. Most E. coli strains were resistant to common antibiotics, and 64% tested positive for ESBL. Interestingly, the majority of E. coli strains had both ESBL and virulence factor genes. The findings highlight a concerning increase in ESBL-producing UPEC and multidrug-resistant drugs in young children, emphasizing the need for further monitoring of antibiotic use in Kurdistan, Iraq.
Article
Microbiology
Katharina Meissner, Carola Sauter-Louis, Stefan E. Heiden, Katharina Schaufler, Herbert Tomaso, Franz J. Conraths, Timo Homeier-Bachmann
Summary: Antimicrobial resistance is a growing global problem in the treatment of bacterial infections in animals and humans. This study found that ESBL-positive E. coli, especially of the CTX-M type, were present in both conventional and organic pig farms. The percentage of ESBL-positive pens was higher on conventional farms and varied greatly on organic farms. The farms of origin appeared to have a major influence on the occurrence of ESBL-producing E. coli in the fattening farms.
Article
Infectious Diseases
Josman Dantas Palmeira, Marisa Haenni, Jean-Yves Madec, Helena Maria Neto Ferreira
Summary: The study identified a high proportion of ESBL-producing E. coli in sheep feces from a farm in southern Portugal. The dissemination of these resistant strains between animals was dynamic and diverse, highlighting the need for global surveillance in all food-producing sectors to prevent the spread of genes conferring resistance to last-resort antibiotics in human medicine.
Article
Agriculture, Dairy & Animal Science
Zui Wang, Qin Lu, Xiaohui Mao, Li Li, Junfeng Dou, Qigai He, Huabin Shao, Qingping Luo
Summary: ESBL-producing E.coli strains isolated from chickens in central China showed high resistance to cephalosporins and harbored diverse ESBL-encoding genes. These strains pose a significant public health risk.
Article
Otorhinolaryngology
Ulrica Thunberg, Amanj Saber, Bo Soderquist, Svante Hugosson
Summary: This study conducted a long-term follow-up of chronic rhinosinusitis patients, finding that symptoms generally decreased and quality of life improved over time. Phenotypic changes were observed in some patients, emphasizing the importance of topical corticosteroids and saline rinsing in treatment.
ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY
(2021)
Article
Genetics & Heredity
Emeli Mansson, Thor Bech Johannesen, Asa Nilsdotter-Augustinsson, Bo Soderquist, Marc Stegger
Summary: The study highlights the differences between S. epidermidis causing prosthetic joint infections and commensal isolates in nasal flora, with the former exhibiting traits associated with resistance to compounds used in infection prevention. A significant portion of PJI isolates did not belong to previously described major nosocomial lineages, but still showed an overrepresentation of antimicrobial resistance-related traits. These findings suggest that current strategies for preventing PJIs may inadvertently select for multidrug-resistant lineages of S. epidermidis that have emerged from horizontal gene transfer.
MICROBIAL GENOMICS
(2021)
Article
Microbiology
Emeli Mansson, Staffan Tevell, Asa Nilsdotter-Augustinsson, Thor Bech Johannesen, Martin Sundqvist, Marc Stegger, Bo Soderquist
Summary: This study found that some patients planned for total joint arthroplasty were colonized with multidrug-resistant S. epidermidis (MDRSE) lineages associated with prosthetic joint infections (PJIs) in their nasal and skin microbiota before hospitalization. This colonization may be influenced by the current prophylaxis regimen. Future modifications to prophylaxis may be necessary, pending further investigation into possible perioperative transmission events and specific risk factors for MDRSE PJIs.
Article
Microbiology
Ulrica Thunberg, Svante Hugosson, Ralf Ehricht, Stefan Monecke, Elke Mueller, Yang Cao, Marc Stegger, Bo Soderquist
Summary: This study analyzed Staphylococcus aureus diversity, genetic factors, and humoral immune responses in chronic rhinosinusitis (CRS) patients through long-term follow-up. The results showed that some patients retained S. aureus isolates from the same genetic lineage over a decade, but the overall changes in antibody responses to staphylococcal antigens were highly variable, with no correlation found between antigen-encoding genes and corresponding antibody levels in serum.
Article
Public, Environmental & Occupational Health
C. Wistrand, B. Soderquist, A-S Sundqvist
Summary: The study demonstrates that protecting sterile goods in the operating room with sterile covers can enhance their durability, benefiting patient safety and quality of care.
JOURNAL OF HOSPITAL INFECTION
(2021)
Article
Multidisciplinary Sciences
Susanna Sagerfors, Anja Poehlein, Mastaneh Afshar, Birgitta Ejdervik Lindblad, Holger Bruggemann, Bo Soderquist
Summary: Infectious keratitis is a potentially sight threatening ophthalmological emergency often associated with contact lens wear. Corynebacterium macginleyi may be considered a corneal pathogen, and genomic analysis can provide insights into its population structure and disease-causing potential.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Sebastian Simon, Bernhard J. H. Frank, Susana Hartmann, Laetitia Hinterhuber, Michael Reitsamer, Alexander Aichmair, Martin Dominkus, Bo Soderquist, Jochen G. Hofstaetter
Summary: This study compared the treatment outcome of dalbavancin with Standard of Care (SoC) in hip and knee periprosthetic joint infections (PJIs). The results showed that dalbavancin treatment resulted in a similar outcome to SoC, with excellent safety and low rate of adverse effects.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Multidisciplinary Sciences
Amaya Campillay Lagos, Martin Sundqvist, Fredrik Dyrkell, Marc Stegger, Bo Soderquist, Paula Molling
Summary: This study evaluated the genomic variation rate in methicillin-resistant Staphylococcus aureus (MRSA) using core genome multilocus sequencing (cgMLST) and single nucleotide polymorphism (SNP) analyses. The results suggest an estimated genomic variation rate of 2.0-5.8 genetic events per year in MRSA.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Ronja Westberg, Marc Stegger, Bo Soderquist
Summary: This study investigated the molecular epidemiology of Staphylococcus haemolyticus isolated from neonatal blood at a Swedish neonatal intensive care unit over 4 decades. It revealed a clonal outbreak in the 1990s and the presence of multidrug resistance and virulence genes. The study emphasizes the importance of restrictive antibiotic use and following hygiene procedures to prevent future endemic outbreaks.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Jasmina Al Janabi, Staffan Tevell, Raphael Niklaus Sieber, Marc Stegger, Bo Soderquist
Summary: This study investigated the susceptibility of Staphylococcus epidermidis strains from prosthetic joint infections to dalbavancin and the genomic variation in strains with reduced susceptibility. The results suggest that exposure to dalbavancin may lead to reduced susceptibility through selection of pre-existing subpopulations, epigenetic changes, or spontaneous mutations during antibiotic exposure. Therefore, source control and adequate antibiotic concentrations are important to prevent emerging reduced susceptibility during dalbavancin treatment.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Letter
Infectious Diseases
Sebastian Simon, Bernhard J. H. Frank, Susana Hartmann, Alexander Aichmair, Bo Soderquist, Jochen G. Hofstaetter
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Bo Soderquist, Thomas Henningsson, Marc Stegger
Summary: Arthroplasty surgery is a common procedure that improves quality of life, but prosthetic joint infection (PJI) is a feared complication, especially after revision surgery. A case of PJI caused by Corynebacterium striatum, an unusual pathogen usually found on human skin, was successfully treated with debridement, antibiotics, and implant retention (DAIR) followed by long-term dalbavancin therapy. Genomic sequencing revealed genetic differences among the C. striatum isolates, but they all had resistance-related genes.
Article
Infectious Diseases
Anna Lange, Ulrica Thunberg, Bo Soderquist
Summary: This case report highlights the importance of caution when administering dalbavancin beyond approved dosing guidelines, and emphasizes the need for vigilance regarding the potential for ototoxicity.
BMC INFECTIOUS DISEASES
(2023)
Article
Ophthalmology
Susanna Sagerfors, Sofie Edslev, Birgitta Ejdervik Lindblad, Berit Lilje, Marc Stegger, Bo Soderquist
Summary: This study aimed to describe bacterial findings from corneal samples of patients with microbial keratitis using targeted sequencing, and to identify factors influencing culture outcome of indirectly inoculated corneal samples.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2023)
Article
Medicine, General & Internal
Mazen A. Sid Ahmed, Jemal M. Hamid, Ahmed A. Husain, Hamad Abdel Hadi, Sini Skariah, Ali A. Sultan, Emad Bashir Ibrahim, Abdul Latif Al Khal, Bo Soderquist, Jana Jass, Ali S. Omrani
Summary: Bloodstream infections caused by multidrug-resistant Pseudomonas aeruginosa in Qatar are relatively uncommon but highly resistant, with colistin being the only consistent effective treatment. Typical risk factors for MDR infections were prevalent in the study population, and clinical outcomes were generally unfavorable.
ANNALS OF MEDICINE
(2021)
