Journal
BMC MICROBIOLOGY
Volume 13, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/1471-2180-13-287
Keywords
Enterovirus 71; Vaccine; VP4; Peptide; Chimeric virus-like particle
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Funding
- International Science & Technology Cooperation [2011DFG33200]
- National High Technology Research and Development Program of China (863 Program) [2012AA02A400]
- Program for New Century Excellent Talents in University [JU015001201001]
- Jilin Program for Development of Science and Technology [20106043]
- Beijing Municipal Education Committee Foundation [JJ015001201301]
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Background: Enterovirus 71 (EV71) is major cause of hand, foot and mouth disease. Large epidemics of EV71 infection have been recently reported in the Asian-Pacific region. Currently, no vaccine is available to prevent EV71 infection. Results: The peptide (VP4N20) consisting of the first 20 amino acids at the N-terminal of VP4 of EV71 genotype C4 were fused to hepatitis B core (HBcAg) protein. Expression of fusion proteins in E. coli resulted in the formation of chimeric virus-like particles (VLPs). Mice immunized with the chimeric VLPs elicited anti-VP4N20 antibody response. In vitro microneutralization experiments showed that anti-chimeric VLPs sera were able to neutralize not only EV71 of genotype C4 but also EV71 of genotype A. Neonatal mice model confirmed the neutralizing ability of anti-chimeric VLPs sera. Eiptope mapping led to the identification of a core sequence responsible for antibody recognition within the peptide. Conclusions: Immunization of chimeric VLPs is able to elicit antibodies displaying a broad neutralizing activity against different genotypes of EV71 in vitro. The core sequence of EV71-VP4 is highly conserved across EV71 genotypes. The chimeric VLPs have a great potential to be a novel vaccine candidate with a broad cross-protection against different EV71 genotypes.
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