Journal
BMC MICROBIOLOGY
Volume 13, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1471-2180-13-13
Keywords
Candiduria; High resolution melting; Genotyping
Categories
Funding
- Astellas Pharma
- bioMerieux
- Gilead Sciences
- Merck Sharp and Dohme
- Pfizer
- Schering Plough
- Soria Melguizo SA
- European Union
- ALBAN program
- Spanish Agency for International Cooperation
- Spanish Ministry of Culture and Education
- Spanish Health Research Fund
- Instituto de Salud Carlos III
- Ramon Areces Foundation
- Mutua Madrilena Foundation
- Spanish Fondo de Investigaciones Sanitarias of the Instituto de Salud Carlos III [PI09/1791, PI11/00412]
- Spanish Network for Research on Infectious Diseases [REIPI RD06/0008/10]
- Fondo de Investigaciones Biomedicas of the Spanish Ministry of Science and Innovation [FI10/00464]
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Background: Several genotyping protocols have been described to study Candida albicans strains with different sensitivity values. In this study we have analyzed the genetic relatedness and the antifungal susceptibility of several Candida albicans strains isolated from a patient who from suffered recurrent candiduria for a period of five years. Strains were genotyped using Microsatellite Length Polymorphism (MLP) with three microsatellite markers (HIS 3, EF 3 and CDC 3), and a new method based on high resolution melting (HRM) was developed to analyze the microsatellite region. This method was compared with the conventional technique that uses capillary electrophoresis. Results: MICs of the isolates showed the existence of fluconazole susceptible and resistant strains. An inter-colony test using single concentration (8 and 16 mg/l) of fluconazole revealed the coexistence of both fluconazole susceptible and resistant strains. Both genotyping analysis methods showed that all the patient's isolates had a clonal origin. HRM analysis method developed was able to accurately establish strain relatedness and presented a discriminatory power of 0.77. Conclusions: Although HRM analysis method presented a lower discriminatory power compared to methods based on capillary electrophoresis, it provided a more cost-effective and suitable alternative for genotyping C. albicans in a clinical laboratory.
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