Article
Food Science & Technology
Babak Pakbin, Wolfram Manuel Bruck, Samaneh Allahyari, John W. A. Rossen, Razzagh Mahmoudi
Summary: This study investigated the prevalence, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of C. sakazakii strains isolated from powdered infant formula milk (PIFM) samples collected in Tehran, Iran. The results showed a high prevalence rate of C. sakazakii in PIFM samples, with high resistance to multiple antibiotics and a diverse genetic profile. The study highlights the need for novel microbial surveillance systems to control the contamination of this foodborne pathogen in infant foods.
Article
Biotechnology & Applied Microbiology
Yan Ma, Yingying Zhang, Ke Chen, Lingzhu Zhang, Yibei Zhang, Xin Wang, Xiaodong Xia
Summary: The study reveals that the PhoP/PhoQ system plays a crucial role in the growth and survival of C. sakazakii under stress conditions, regulating resistance to environmental stress by affecting multiple gene pathways.
Article
Biotechnology & Applied Microbiology
Jie Zhan, Jun Qiao, Xiaoyuan Wang
Summary: The study found that the rpoS gene of Cronobacter sakazakii is involved in environmental stress resistance, affecting membrane formation, auto-aggregation, motility, acid resistance, and hyperosmotic resistance of the bacteria. Transcriptional analysis also showed differential expression of resistance-related genes and changes in metabolic pathways.
Article
Agriculture, Dairy & Animal Science
Hui Li, Yi Zhao, Jingjing Zhang, Wen Li, Qixiu You, Xianxiang Zeng, Hengyi Xu
Summary: This study investigated the effects of silver nanoparticles (AgNP) on the environmental tolerance and biofilm formation of Cronobacter sakazakii. The results showed that AgNP significantly reduced the survival rate of C. sakazakii under environmental stress conditions, inhibited biofilm formation, and downregulated the expression of genes related to stress tolerance. AgNP also inhibited ATP production and biofilm formation in C. sakazakii cells.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Food Science & Technology
Zhanwen Zheng, Yawen Xie, Sheng Ma, Junhong Tu, Jiahui Li, Sen Liang, Yunfeng Xu, Chao Shi
Summary: The study demonstrated that 405-nm LED light effectively inactivated C. sakazakii in PIF, reducing its tolerance to various environmental stressors. LED illumination also downregulated the transcription levels of tolerance-associated genes and caused damage to bacterial cell membranes. This suggests the potential of 405-nm LED technology in preventing and controlling pathogens in food processing and storage environments.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Jae-Ik Lee, Sang-Soon Kim, Dong-Hyun Kang
Summary: The aim of this study was to develop a new primer-probe set for detecting Cronobacter sakazakii based on comparative genomics. The sensitivity and selectivity of the designed primer were confirmed, and it was found to have minimal interference from other substances. Additionally, the detection efficiency of the designed primer in various food samples was comparable to that of conventionally used selective media.
Article
Chemistry, Analytical
Xu Chen, Yue Ma, Shuangyu Miao, Dongnan Li, Ye Zhang
Summary: In this study, a multiplex competitive annealing mediated isothermal amplification (mCAMP) assay on a microfluidic chip was developed for the visual detection of Cronobacter sakazakii in powdered infant formula (PIF) samples. The assay showed high specificity and a limit of detection of 2.2 x 10(3) CFU g(-1). The results are visible to the naked eye, making it suitable for rapid analysis in resource-limited settings.
Article
Biotechnology & Applied Microbiology
Jiri Novotny, Barbora Svobodova, Jiri Santrucek, Ladislav Fukal, Ludmila Karamonova
Summary: This study presents an improved method for mapping the proteome of Cronobacter sakazakii, a genus responsible for severe infections. By fractionating the bacterial cells and using a novel protein identification approach, they identified a large number of proteins, providing important insights into the pathogenesis and virulence factors of this bacterium.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Food Science & Technology
Xinrui Lv, Xiaokui Gu, Li Wang, Xiaoxin He, Chunai He, Jingfeng Zhang, Lichao Zhao
Summary: This study developed an improved PMAxx-SIC ddPCR method for rapid and absolute quantification of Cronobacter sakazakii in VBNC state. The method showed higher detection sensitivity and accuracy compared to real-time PCR, and demonstrated the potential of underestimating microbial population by conventional culture-based methods.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Xuemeng Ji, Ping Lu, Yaozhong Hu, Juan Xue, Jing Wu, Bowei Zhang, Yan Zhang, Lu Dong, Huan Lv, Shuo Wang
Summary: This study successfully deleted endogenous plasmids in Cronobacter sakazakii using a suicide vector, confirming their contribution to invasion, virulence, and multidrug resistance. The findings suggest that p-coumaric acid may be a safe and effective plasmid-curing agent at sub-inhibitory concentrations.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Sambit K. Das, Sanath H. Kumar, Binaya B. Nayak, Manjusha Lekshmi
Summary: This study investigated the incidence of Cronobacter spp. in seafood from retail fish markets in Mumbai, India. It found the highest incidence of Cronobacter spp. in dried fish, with Cronobacter malonaticus being the most common species identified. The detection of Cronobacter spp. highlights the importance of monitoring this emerging pathogen in tropical seafood.
CURRENT MICROBIOLOGY
(2021)
Article
Immunology
Xuemeng Ji, Ping Lu, Juan Xue, Ning Zhao, Yan Zhang, Lu Dong, Xuejiao Zhang, Ping Li, Yaozhong Hu, Jin Wang, Bowei Zhang, Jingmin Liu, Huan Iv, Shuo Wang
Summary: Cronobacter sakazakii, an emerging opportunistic pathogen, is associated with severe foodborne outbreak infections in both premature and full-term infants. This study identified the nlpD gene as an acid-resistance factor in C. sakazakii, playing a vital role in maintaining membrane integrity and virulence. The findings suggest that targeting NlpD could be a potential strategy for drug development against C. sakazakii.
Article
Food Science & Technology
Mohd Izhar Ariff Mohd Kashim, Alia Aryssa Abdul Haris, Nur Asmadayana Hasim, Sahilah Abd Mutalib, Nurina Anuar
Summary: The aim of this research was to determine the halal status of cultured meat by detecting species-specific DNA of bovine serum. The results revealed that bovine DNA was detected in all samples tested, indicating that perfect transformation is not possible and is prohibited according to Shariah law.
Article
Biochemistry & Molecular Biology
Jie Zhan, Xin Tan, Xiaoyuan Wang
Summary: The study investigated the role of stringent starvation protein A (SspA) in stress tolerance of Cronobacter sakazakii BAA894. Results showed that knockout of sspA affected the bacterium's tolerance to various stress factors, providing insight into its pathogenicity and stress response mechanisms. Transcriptional analysis revealed significant gene expression changes in the mutant strain, contributing to a better understanding of how SspA influences stress tolerance in C. sakazakii BAA894.
CANADIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Plant Sciences
Hongli Wang, Xi Cheng, Qiyu Shi, Jie Xu, Dongliang Chen, Chang Luo, Hua Liu, Li Cao, Conglin Huang
Summary: This study evaluated the cold tolerance of Rosa materials and identified genes related to cold tolerance. Through cold treatment, it was found that the most cold-tolerant materials were Rosa hybrida, Rosa rugosa 'Pingyin 12', and Rosa rugosa. RNA-Seq analysis identified 204 significantly differentially expressed genes (DEGs), with 88 genes significantly up-regulated and 116 genes significantly down-regulated under cold conditions. Gene ontologies and pathway analyses revealed enrichment in 57 pathways.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Microbiology
Paul Tetteh Asare, Katrin Zurfluh, Anna Greppi, Denise Lynch, Clarissa Schwab, Roger Stephan, Christophe Lacroix
Article
Microbiology
Valerie Wist, Marina Morach, Marianne Schneeberger, Nicole Cernela, Marc J. A. Stevens, Katrin Zurfluh, Roger Stephan, Magdalena Nuesch-Inderbinen
Article
Food Science & Technology
Martin Laage Kragh, Francis Muchaamba, Taurai Tasara, Lisbeth Truelstrup Hansen
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2020)
Article
Microbiology
Roxana Zamudio, Richard D. Haigh, Joseph D. Ralph, Megan De Ste Croix, Taurai Tasara, Katrin Zurfluh, Min Jung Kwun, Andrew D. Millard, Stephen D. Bentley, Nicolas J. Croucher, Roger Stephan, Marco R. Oggioni
ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Microbiology
Francis Muchaamba, Athmanya K. Eshwar, Ueli von Ah, Marc J. A. Stevens, Taurai Tasara
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Environmental Sciences
Stephanie Bleichenbacher, Marc J. A. Stevens, Katrin Zurfluh, Vincent Perreten, Andrea Endimiani, Roger Stephan, Magdalena Nueesch-Inderbinen
ENVIRONMENTAL POLLUTION
(2020)
Article
Microbiology
Joseph Wambui, Athmanya K. Eshwar, Mariella Aalto-Araneda, Anna Pontinen, Marc J. A. Stevens, Patrick M. K. Njage, Taurai Tasara
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Microbiology
Mirjami Mattila, Panu Somervuo, Hannu Korkeala, Roger Stephan, Taurai Tasara
Article
Food Science & Technology
Francis Muchaamba, Roger Stephan, Taurai Tasara
Article
Food Science & Technology
Francis Muchaamba, Helena Stoffers, Ralf Blase, Ueli von Ah, Taurai Tasara
Summary: The study showed that using potassium lactate as a substitute for sodium chloride can effectively reduce salt content in salami products, mitigating the negative health impacts of a high salt diet. Comparison between the low salt plus KL combination and high salt concentration revealed similar anti-Listeria activity and product characteristics.
Article
Microbiology
Danai Etter, Celine Jenni, Taurai Tasara, Sophia Johler
Summary: This study used strains harboring different SEC variants to investigate the impact of mild lactic acid stress on SEC expression. Results showed that while mRNA levels only slightly decreased under lactic acid stress, protein levels exhibited a significant decrease for some strains, indicating post-transcriptional modifications can influence SEC expression under lactic acid stress.
Article
Microbiology
Joao Anes, Katherine Dever, Athmanya Eshwar, Scott Nguyen, Yu Cao, Sathesh K. Sivasankaran, Sandra Sakalauskaite, Angelika Lehner, Stephanie Devineau, Rimantas Daugelavicius, Roger Stephan, Seamus Fanning, Shabarinath Srikumar
Summary: This study induced oxidative stress in K. pneumoniae MGH78578 using paraquat and identified the soxS gene as significantly induced during oxidative stress, potentially serving as a genetic target against multidrug-resistant K. pneumoniae infections. The soxS mutant exhibited reduced susceptibility to tetracycline, with impaired efflux activity leading to tetracycline accumulation in the cytoplasm. The study suggests that targeting the soxS gene could be a promising strategy for combating antimicrobial resistance in K. pneumoniae.
Article
Microbiology
Magdalena Nueesch-Inderbinen, Claudia Haenni, Katrin Zurfluh, Sonja Hartnack, Roger Stephan
Summary: This study compared antimicrobial resistance among commensal Escherichia coli in young calves raised on organic and conventional dairy farms in Switzerland. The results showed that antimicrobial resistance was highly prevalent in both farming systems, but certain resistance phenotypes were higher in organic calves. Additionally, ESBL-producing Enterobacteriaceae were only detected in samples from conventionally farmed calves.
Article
Microbiology
Hyein Jang, Athmanya Eshwar, Angelika Lehner, Jayanthi Gangiredla, Isha R. Patel, Junia Jean-Gilles Beaubrun, Hannah R. Chase, Flavia Negrete, Samantha Finkelstein, Leah M. Weinstein, Katie Ko, Nicole Addy, Laura Ewing, Jungha Woo, Youyoung Lee, Kunho Seo, Ziad Jaradat, Shabarinath Srikumar, Seamus Fanning, Roger Stephan, Ben D. Tall, Gopal R. Gopinath
Summary: Comparative molecular and genomic analysis, as well as zebrafish infection studies, were conducted on plant-origin Cronobacter sakazakii strains. It was found that these strains possess genomic features and virulence comparable to clinical strains, indicating that these foods could potentially serve as transmission vehicles for this important foodborne pathogen.
Article
Agriculture, Dairy & Animal Science
Taurai Tasara, Anja Barbara Meier, Joseph Wambui, Ronan Whiston, Marc Stevens, Aspinas Chapwanya, Ulrich Bleul
Summary: Research on microorganisms in the reproductive tract of cows has gained popularity. Pathogens in the reproductive tract, such as bacteria, contribute to uterine disease and decreased fertility. By comparing the microbiomes of healthy and metritis animals, our study has identified microbiome profiles that are linked to uterine health. This knowledge can help develop treatment options that reduce antibiotic use and improve fertility in cows, as well as identify animals capable of successful pregnancy after calving.