Article
Multidisciplinary Sciences
Jeongjoon Choi, Matias Schmukler, Eduardo A. Groisman
Summary: Horizontal gene transfer drives bacterial evolution, and H-NS proteolysis is a mechanism essential for derepressing foreign genes and colonization of mammalian hosts.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Alejandro Huerta-Saquero, Itziar Chapartegui-Gonzalez, Sarah Bowser, Nittaya Khakhum, Jacob L. Stockton, Alfredo G. Torres
Summary: This study demonstrates the potential of using P22 viral-like particles as nanocontainers for delivering immunogenic proteins of enterohemorrhagic Escherichia coli (EHEC) and creating effective vaccines against EHEC infections. The EscC-P22 particles were able to induce specific antibodies in mice, enhance phagocytic activity against EHEC, and reduce bacterial adherence to epithelial cells, indicating their functionality. These findings suggest that EscC-P22-based particles could be a promising nanovaccine candidate for immunization against EHEC O157:H7 infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Lang Peng, Rexford Mawunyo Dumevi, Marco Chitto, Nadja Haarmann, Petya Berger, Gerald Koudelka, Herbert Schmidt, Alexander Mellmann, Ulrich Dobrindt, Michael Berger
Summary: This study proposes a new method for genetic manipulation of enterohemorrhagic Escherichia coli (EHEC) that is more efficient than traditional methods. The method increases recombineering efficiency by 3-4 times in EHEC O157:H7 EDL933 Delta stx1/2, but shows similar efficiency in Escherichia coli K-12 MG1655. Additionally, the study suggests that the inhibition of the RecFOR pathway indirectly blocks phage induction and improves recombineering efficiency.
Article
Chemistry, Analytical
Anneliese M. M. Faustino, Piyoosh Sharma, Edgar Manriquez-Sandoval, Divya Yadav, Stephen D. D. Fried
Summary: Cross-linking mass spectrometry (XL-MS) is a promising method for studying protein-protein interactions on a large scale and with high resolution. However, the use of photo-cross-linkers in proteome-wide studies has been limited due to challenges in identifying their products. In this study, we demonstrate the synthesis and application of two water-soluble and cell-permeable photo-cross-linkers, which can form MS-cleavable linkages with protein targets. These cross-linkers enable proteome-wide photo-cross-linking in live cells and provide residue-level resolution for analyzing protein interaction networks. Further optimization of these methods may lead to a better understanding of the molecular sociology of the cell.
ANALYTICAL CHEMISTRY
(2023)
Review
Gastroenterology & Hepatology
Lingyan Jiang, Wen Yang, Xinlei Jiang, Ting Yao, Lu Wang, Bin Yang
Summary: EHEC O157 carries 177 unique genomic islands, termed O islands, which play a crucial role in its pathogenicity by providing virulence factors, effectors, and regulatory proteins.
Review
Food Science & Technology
Xiaoyan Zhao, Yeting Sun, Yue Ma, Yujia Xu, Hongyang Guan, Dan Wang
Summary: Enterohemorrhagic Escherichia coli (EHEC) is a major bacterial pathogen that can survive in food such as meat and vegetables. Understanding the impact of vegetable characteristics on the adhesion and proliferation processes of EHEC is important for developing control measures. This review discusses the amount, methods, and pathways of adhesion, as well as the proliferation and biofilm formation processes of EHEC during vegetable contamination. Factors such as vegetable types, cultivars, tissue characteristics, and damage levels can affect EHEC adhesion on vegetables.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Veterinary Sciences
Delphine Bibbal, Philippe Ruiz, Panagiotis Sapountzis, Christine Mazuy-Cruchaudet, Estelle Loukiadis, Frederic Auvray, Evelyne Forano, Hubert Brugere
Summary: This study aimed to identify farms with persistent circulation of EHEC O157:H7 in cattle. The results showed that persistent circulation may be due to the presence of a few predominant strains or repeated exposure of cattle to various strains. In addition, differences were found in the fecal microbial communities of shedders and non-shedders, with different bacterial indicators identified.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Joshua L. Cherry
Summary: Enterohemorrhagic E. coli (EHEC) is a major cause of human illness, death, and economic loss. Understanding the genetic changes in EHEC and its source of contamination is crucial for effective mitigation. Through whole-genome sequencing, it was found that EHEC experienced genetic changes mainly in adhesion-related sequences, potentially leading to altered adhesive properties and increased virulence. This pattern of genetic change may help identify other bacteria threatening human health. Additionally, the presence of a mutation affecting arsenic resistance regulation suggests an arsenic-contaminated groundwater as the persistent source of contamination. This observation can aid in locating the reservoir of EHEC.
MICROBIOLOGY SPECTRUM
(2022)
Article
Food Science & Technology
So Young Yang, Ki Sun Yoon
Summary: Listeria monocytogenes and Entero-hemorrhagic Escherichia coli (EHEC) can survive in refrigerated yogurt. The risk of L. monocytogenes and EHEC due to yogurt consumption was estimated using predictive survival models. The results show that the foodborne illness risk from L. monocytogenes and EHEC due to yogurt consumption is very low, but controlling the initial contamination level of EHEC during yogurt manufacture should be emphasized.
Article
Multidisciplinary Sciences
Natalia O. Dranenko, Maria N. Tutukina, Mikhail S. Gelfand, Fyodor A. Kondrashov, Olga O. Bochkareva
Summary: The pathogenicity of Shigella and enteroinvasive Escherichia coli is based on the type 3 secretion system (T3SS) encoded by the pINV virulence plasmid. Recent genomic studies have found ipaH genes in potential marmot pathogens and E. coli from fecal samples of bovine calves, suggesting that non-human hosts may also be infected by these strains. Comparative genomic analysis revealed gene losses, duplications, and inconsistencies between strains phylogeny and composition of effectors, indicating horizontal gene transfer between E. coli adapted to different hosts.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Jun-Young Park, Cheorl-Ho Kim, Seung-Hak Cho
Summary: In this study, we developed a FimH inhibitor to prevent adhesion of enterohemorrhagic Escherichia coli on human intestinal epithelium during the early stage of infection. By investigating the ligand binding domain of FimH and analyzing its evolutionary characteristics, we identified a potential drug target and found that glycan-like peptides can serve as initial infection inhibitors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Food Science & Technology
Yansong Xue, Mei-Jun Zhu
Summary: This article discusses how enterohemorrhagic Escherichia coli (EHEC), specifically E. coli O157:H7, hijacks the host immune system and interferes with host signaling pathways. It also highlights the promising role of bioactive compounds derived from food and probiotics in harnessing host innate immunity and eliminating E. coli O157:H7 infection with multiple strategies.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Multidisciplinary Sciences
Diana Rodriguez-Valverde, Jorge A. Giron, Yang Hu, James P. Nataro, Fernando Ruiz-Perez, Araceli E. Santiago
Summary: ANR proteins, including Aar and Rnr, are small regulatory proteins commonly found in enteric pathogens, which play important roles in regulating virulence-related genes and colonization of the intestine. Our study reveals the regulatory mechanisms of Aar and Rnr in two different types of enteropathogens, and highlights the conserved role of ANR in modulating intestinal colonization.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Dawei Yang, Yongwu Yang, Pengfei Qiao, Fengwei Jiang, Xinyang Zhang, Zihui Zhao, Tao Cai, Ganwu Li, Wentong Cai
Summary: This study identified a two-component signaling system in EHEC that promotes adaptability and virulence expression in response to mannose, a sugar found in the intestinal mucus.
Article
Biochemistry & Molecular Biology
Suchintak Dash, Cristina S. D. Palma, Ines S. C. Baptista, Bilena L. B. Almeida, Mohamed N. M. Bahrudeen, Vatsala Chauhan, Rahul Jagadeesan, Andre S. Ribeiro
Summary: The study investigated the underlying mechanisms of Escherichia coli cold shock response. It was found that some cold shock repressed genes could be triggered by changes in DNA supercoiling. Additionally, the response strengths to cold shock and gyrase inhibition were correlated, and cold shock led to an increase in nucleoid density and a decrease in cellular energy.
NUCLEIC ACIDS RESEARCH
(2022)