Article
Immunology
Mariana Izquierdo, Joaquin Lopez, Pablo Gallardo, Roberto M. Vidal, Juan C. Ossa, Mauricio J. Farfan
Summary: This study evaluated the role of Citrobacter werkmanii and Escherichia albertii in the virulence of Shiga toxin-producing and enteroaggregative Escherichia coli pathotypes, revealing their effects on gene expression and toxin secretion.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Hyeonjeong Lee, Hyunsoo Han, Sangmin Jeon
Summary: In this study, a novel method for efficiently capturing waterborne bacteria was developed by creating an autonomous internal reflux of magnetic nanoparticle chains. By placing a glass tube containing positively charged PEI-coated MNCs at the center of a Halbach ring, a strong and uniform magnetic field was generated to capture target bacteria via electrostatic interaction. The capture efficiency of E. coli O157 was found to be over 90% at a flow rate of 15 mL/min, with detection limits of 2 and 3 cfu/mL in deionized water and river water, respectively.
ANALYTICAL CHEMISTRY
(2021)
Article
Immunology
Claudia A. A. Freire, Beatriz O. O. Rodrigues, Waldir P. P. Elias, Cecilia M. M. Abe
Summary: This study aimed to evaluate the presence of AAF, AFP, and CS22-related genes in EAEC strains collected from children with diarrhea. The results indicate that AAF and AFP-related genes may contribute to identify EAEC strains, while the importance of cseA as an EAEC virulence factor and genotypic marker needs further evaluation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Angelina A. Kislichkina, Nikolay N. Kartsev, Yury P. Skryabin, Angelika A. Sizova, Maria E. Kanashenko, Marat G. Teymurazov, Ekaterina S. Kuzina, Alexander G. Bogun, Nadezhda K. Fursova, Edward A. Svetoch, Ivan A. Dyatlov
Summary: Hybrid diarrheagenic E. coli strains with genetic markers from different pathotypes have emerged as a public health concern. The most well-known hybrid strain, E. coli O104:H4, caused a serious outbreak of acute gastroenteritis and hemolytic uremic syndrome (HUS) in Germany in 2011. A recent case of HUS intestinal infection was reported in St. Petersburg, Russia, with the E. coli strain SCPM-O-B-9427, which shares similarities with other related strains.
Article
Pharmacology & Pharmacy
Vemula Prasastha Ram, Jyothsna Yasur, Padikkamannil Abishad, Varsha Unni, Diksha Purushottam Gourkhede, Maria Anto Dani Nishanth, Pollumahanti Niveditha, Jess Vergis, Satya Veer Singh Malik, Byrappa Kullaiah, Nitin Vasantrao Kurkure, Chatragadda Ramesh, Laurent Dufosse, Deepak B. Rawool, Sukhadeo B. Barbuddhe
Summary: This study investigated the antimicrobial efficacy of nanosilver-entrapped cinnamaldehyde (AgC) against multi-drug-resistant strains of enteroaggregative Escherichia coli (EAEC). The results showed that AgC had a high stability, safety, and effectiveness in inhibiting and killing MDR-EAEC. In vivo experiments using Galleria mellonella larvae also confirmed the antibacterial and immunomodulatory effects of AgC.
Article
Microbiology
Maria Teresa Llorente, Raquel Escudero, Raquel Ramiro, Maria Antonia Remacha, Rocio Martinez-Ruiz, Fatima Galan-Sanchez, Monica de Frutos, Matilde Elia, Isabel Onrubia, Sergio Sanchez
Summary: This study aimed to investigate the occurrence of enteroaggregative Escherichia coli (EAEC) in patients with non-travel related diarrhea in Spain and to characterize the molecular features of EAEC strains associated with illness in this high-income setting. The results showed that EAEC was the only bacterial enteric pathogen detected in a significant proportion of cases of endemic diarrhea in Spain, especially in children under 5 years old. Some of these strains also had a potential pathogenicity in the urinary/systemic system.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yuqi Yang, Ping Cheng, Tianshi Xiao, Jargalsaikhan Ulziikhutag, Hongxiao Yu, Jiarui Li, Ruimeng Liu, Ishfaq Muhammad, Xiuying Zhang
Summary: Improper use of antibiotics can lead to poor treatment effectiveness and increased bacterial resistance. In this study, ultrafiltration probes were placed in the ileum of piglets with the help of anesthesia. The concentration of DAN in the ileum ultrafiltrate was significantly higher than in plasma, and PK parameters for both compartments were calculated using WinNonlin software. The study also found that the killing mechanism of DAN against E. coli is concentration-dependent, providing insights for rational dosage schedule design.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Romana R. Gerner, Suzana Hossain, Artur Sargun, Kareem Siada, Grant J. Norton, Tengfei Zheng, Wilma Neumann, Sean-Paul Nuccio, Elizabeth M. Nolan, Manuela Raffatellu
Summary: Inflammatory bowel diseases (IBD) are characterized by chronic inflammation of the gastrointestinal tract and alterations to the gut microbiome. This study found that immunization targeting siderophores reduced the colonization and association of adherent-invasive Escherichia coli (AIEC) with the gut mucosa, leading to a reduction in colitis severity. The study also laid the foundation for developing monoclonal antibodies against siderophores as a potential therapeutic strategy.
Article
Biochemistry & Molecular Biology
Jacqueline Hemberger, Julia Ittensohn, Hannah Griffiths, Maren Keller, Victor Costina, Simone Albrecht, Thomas Miethke
Summary: TcpC protein of uropathogenic Escherichia coli strain CFT073 modulates innate immunity by interfering with Toll-like receptor and NALP3 inflammasome signaling cascade. Different cell types, salts and bacterial density can activate the tcpC promoter, explaining the mechanism of TcpC induction during kidney infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Waleska Saitz, David A. Montero, Mirka Pardo, Daniela Araya, Marjorie De la Fuente, Marcela A. Hermoso, Mauricio J. Farfan, Daniel Ginard, Ramon Rossello-Mora, Dave A. Rasko, Felipe Del Canto, Roberto M. Vidal
Summary: The study aimed to identify molecular markers for screening AIEC strains. By analyzing outer membrane proteins and genes in AIEC strains, the study found that chuA, eefC, and fitA genes are significantly correlated with AIEC strains and can serve as markers for AIEC strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Alejandro Prieto, Manuel Bernabeu, Jose Francisco Sanchez-Herrero, Anna Perez-Bosque, Lluisa Mir, Christine Bauer, Carmen Colladcy, Mario Huttener, Antonio Juarez
Summary: Research on Enteroaggregative Escherichia coli (EAEC) has uncovered a novel mechanism involving the 3' UTR of the gene that encodes the transcriptional activator AggR, which affects aggR transcript stability and AggR levels, ultimately impacting EAEC virulence. Additionally, the study has identified new AggR-regulated genes that could play significant roles in EAEC virulence.
COMMUNICATIONS BIOLOGY
(2021)
Article
Immunology
Mireia Lopez-Siles, Carla Camprubi-Font, Eva M. Gomez del Pulgar, Miriam Sabat Mir, David Busquets, Yolanda Sanz, Margarita Martinez-Medina
Summary: The study found that adherent-invasive Escherichia coli (AIEC) may be associated with both Crohn's disease (CD) and ulcerative colitis (UC), but not with colorectal cancer (CRC). Additionally, further research on E. coli strains in celiac disease (CeD) would be of interest.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Xu Zhilu, Dong Xiangqian, Yang Keli, Chevarin Caroline, Zhang Jingwan, Lin Yu, Zuo Tao, Chu Lok Cheung, Sun Yang, Zhang Fengrui, Chan Francis Kl, Sung Joseph Jy, Yu Jun, Buisson Anthony, Barnich Nicolas, Colombel Jean-Frederic, Wong Sunny Hei, Miao Yinglei, Ng C. Siew
Summary: The study found a high prevalence of AIEC in CD patients in Hong Kong, which was associated with severe microbiota dysbiosis and impeded restoration of normal gut microbiota. AIEC may serve as a keystone bacterium in CD and impact the efficacy of FMT.
Article
Immunology
Munirah M. Alhammadi, Rita E. Godfrey, Joseph O. Ingram, Gurdamanjit Singh, Camilla L. Bathurst, Stephen J. W. Busby, Douglas F. Browning
Summary: Serine protease autotransporters of the Enterobacteriaceae (SPATEs) are virulence factors commonly found in enteric bacteria, such as E. coli and Shigella. One specific SPATE called Pic mucinase cleaves mucin, allowing bacteria to utilize it as a carbon source and penetrate the gut lining. The regulation of the pic gene in different strains of E. coli is controlled by the transcription factors CRP and Fis, with CRP activating transcription in nutrient-depleted conditions and Fis repressing expression in nutrient-rich environments. This regulation is consistent with Pic's role in scavenging mucin as a carbon source during colonization and infection.
Article
Engineering, Chemical
Iosif Marios Scoullos, Ioannis Tournis, Evangelos Kouvelos, Zili Sideratou, Andreas Sapalidis
Summary: The formation of biofouling on membrane surfaces is a critical factor affecting the efficiency of desalination processes. This research examines the efficacy of different membranes in inhibiting the adhesion of proteins and bacteria. The results indicate that the adhesion of both agents is stronger on hydrophilic membrane surfaces.
DESALINATION AND WATER TREATMENT
(2022)
Article
Infectious Diseases
Abdurrahman Hassan Jibril, Iruka N. Okeke, Anders Dalsgaard, Vanesa Garcia Menendez, John Elmerdahl Olsen
Summary: Antimicrobial resistance is a global public health concern, and this study focused on identifying antimicrobial resistance and resistance genes in Salmonella isolates from poultry in Nigeria. Phenotypic resistance to commonly used antibiotics was observed, with corresponding antibiotic resistance genes detected in genomes, often carried on specific plasmids. Some Salmonella strains carried multidrug resistance genes, indicating potential for resistance.
Review
Biochemistry & Molecular Biology
El-Shama Q. A. Nwoko, Iruka N. Okeke
Summary: Autoaggregation is an important factor in bacterial survival, biofilm formation, and other functions, regulated by various mechanisms. Understanding and studying autoaggregation through qualitative and quantitative methods can lead to the discovery of new therapeutic targets.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Article
Infectious Diseases
Karen H. Keddy, Senjuti Saha, Samuel Kariuki, John Bosco Kalule, Farah Naz Qamar, Zoya Haq, Iruka N. Okeke
Summary: Despite advancements in management, diarrhoea remains a significant cause of illness and death in children from low-income and middle-income countries (LMICs). Research on the causes of diarrhoea in LMICs has improved, thanks to multinational studies and big data analytics. Mobile phones have also contributed to the management of childhood diarrhoea by providing clinical support to healthcare workers and communicating preventive measures to caregivers.
LANCET INFECTIOUS DISEASES
(2022)
Editorial Material
Biochemistry & Molecular Biology
Iruka N. Okeke
Summary: Health research in low-income settings needs to prioritize sustainability for long-term impact on target diseases. Collaborative efforts from biomedical researchers in high-income countries are essential for achieving this goal.
Editorial Material
Medicine, Research & Experimental
Iruka N. Okeke
AFRICAN JOURNAL OF LABORATORY MEDICINE
(2021)
Article
Medicine, General & Internal
Blake Angell, Olutobi Sanuade, Ifedayo M. O. Adetifa, Iruka N. Okeke, Aishatu Lawal Adamu, Muktar H. Aliyu, Emmanuel A. Ameh, Fatima Kyari, Muktar A. Gadanya, Diana A. Mabayoje, Adesola Yinka-Ogunleye, Tolu Oni, Rabiu Ibrahim Jalo, Fatimah I. Tsiga-Ahmed, Sarah L. Dalglish, Seye Abimbola, Tim Colbourn, Obinna Onwujekwe, Eme Theodora Owoaje, Gambo Aliyu, Sani H. Aliyu, Belinda Archibong, Alex Ezeh, Chikwe Ihekweazu, Zubairu Iliyasu, Stephen Obaro, Ebenezer Babatunde Obadare, Friday Okonofua, Muhammed Pate, Babatunde L. Salako, Fatima H. Zanna, Scott Glenn, Ally Walker, Maha Ezalarab, Mohsen Naghavi, Ibrahim Abubakar
Summary: Despite increased health expenditure in Nigeria, health outcomes remain poor. It is suggested that strengthening the health system and implementing targeted interventions could improve population health.
Review
Medicine, General & Internal
Ibrahim Abubakar, Sarah L. Dalglish, Blake Angell, Olutobi Sanuade, Seye Abimbola, Aishatu Lawal Adamu, Ifedayo M. O. Adetifa, Tim Colbourn, Afolabi Olaniyi Ogunlesi, Obinna Onwujekwe, Eme T. Owoaje, Iruka N. Okeke, Adebowale Adeyemo, Gambo Aliyu, Muktar H. Aliyu, Sani Hussaini Aliyu, Emmanuel A. Ameh, Belinda Archibong, Alex Ezeh, Muktar A. Gadanya, Chikwe Ihekweazu, Vivianne Ihekweazu, Zubairu Iliyasu, Aminatu Kwaku Chiroma, Diana A. Mabayoje, Mohammed Nasir Sambo, Stephen Obaro, Adesola Yinka-Ogunleye, Friday Okonofua, Tolu Oni, Olu Onyimadu, Muhammad Ali Pate, Babatunde L. Salako, Faisal Shuaib, Fatimah Tsiga-Ahmed, Fatima H. Zanna
Review
Microbiology
Odion O. Ikhimiukor, Erkison Ewomazino Odih, Pilar Donado-Godoy, Iruka N. Okeke
Summary: This article describes the transmission of antimicrobial resistance in low- and middle-income countries from a One Health perspective and emphasizes high-risk transmission points such as urban settings and food-animal handling. The authors suggest that combining top-down and bottom-up approaches is necessary to effectively contain AMR in these settings.
NATURE MICROBIOLOGY
(2022)
Article
Medicine, General & Internal
Erkison Ewomazino Odih, Emmanuel Oladayo Irek, Temitope O. Obadare, Anderson O. Oaikhena, Ayorinde O. Afolayan, Anthony Underwood, Anthony T. Adenekan, Veronica O. Ogunleye, Silvia Argimon, Anders Dalsgaard, David M. Aanensen, Iruka N. Okeke, A. Oladipo Aboderin
Summary: This study retrospectively investigated an outbreak of carbapenem-resistant Acinetobacter baumannii (CRAB) in an ICU in Southwest Nigeria, identifying a clone of CRAB belonging to the globally disseminated international clone (IC) 2. The findings highlight the need to strengthen the capacity to detect and control A. baumannii in Nigerian hospitals.
FRONTIERS IN MEDICINE
(2022)
Article
Environmental Sciences
Akeem Ganiyu Rabiu, Olutayo Israel Falodun, Obasola Ezekiel Fagade, Rotimi Ayodeji Dada, Iruka N. Okeke
Summary: This study investigated fecal contamination in household water sources in Ibadan, Nigeria and found significant increase of Escherichia coli during the wet season. The findings suggest that the water sources evaluated are unfit for consumption.
JOURNAL OF WATER AND HEALTH
(2022)
Review
Microbiology
Iruka N. Okeke, Aaron O. Aboderin, Abiodun Egwuenu, Anthony Underwood, Ayorinde O. Afolayan, Mihir Kekre, Anderson O. Oaikhena, Erkison Ewomazino Odih, Hamzat T. Omotayo, Hannah Dada-Adegbola, Veronica O. Ogunleye, Odion O. Ikhimiukor, David M. Aanensen, Chikwe Ihekweazu
Summary: Whole-genome sequencing (WGS) has important applications in antimicrobial resistance (AMR) surveillance, providing detailed data and expanding the scope of surveillance. WGS has replaced traditional strain subtyping and is now the gold standard for quickly identifying isolates in suspected outbreak clusters.
Article
Infectious Diseases
Odion O. Ikhimiukor, Anderson O. Oaikhena, Ayorinde O. Afolayan, Abayomi Fadeyi, Aderemi Kehinde, Veronica O. Ogunleye, Aaron O. Aboderin, Oyinlola O. Oduyebo, Charles J. Elikwu, Erkison Ewomazino Odih, Ifeoluwa Komolafe, Silvia Argimon, Abiodun Egwuenu, Ini Adebiyi, Oluwadamilola A. Sadare, Tochi Okwor, Mihir Kekre, Anthony Underwood, Chikwe Ihekweazu, David M. Aanensen, Iruka N. Okeke
Summary: Invasive salmonellosis in southwest Nigeria is mainly caused by multidrug-resistant, West African dominant S. Typhi lineage 3.1.1, most of which can be prevented through vaccination. However, there are also invasive non-typhoidal Salmonella strains carrying typhoidal toxin or resistance genes.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Infectious Diseases
David A. Kwasi, Chinedum P. Babalola, Olujide O. Olubiyi, Jennifer Hoffmann, Ikemefuna C. Uzochukwu, Iruka N. Okeke
Summary: This study identified five compounds with significant EAEC antibiofilm activity, including one molecule that targets the EAEC Aap protein. Inhibition of EAEC colonization by small molecules provides a potential new therapeutic approach against EAEC infection.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Agriculture, Dairy & Animal Science
Abdurrahman Hassan Jibril, Iruka N. Okeke, Anders Dalsgaard, John Elmerdahl Olsen
Summary: This study investigated the prevalence of Salmonella in hatchlings in Nigeria, with a focus on resistant strains. The study also analyzed the genetic relatedness between hatchling strains and strains from poultry farms. The results showed a high prevalence of Salmonella and a significant presence of multidrug-resistant strains.
Article
Microbiology
Erkison Ewomazino Odih, Gabriel Temitope Sunmonu, Iruka N. Okeke, Anders Dalsgaard
Summary: This study investigated the occurrence of carbapenem-resistant Acinetobacter baumannii in hospital wastewater in Ibadan, Nigeria, and explored the phylogenetic relationships between these strains and clinical isolates. The study revealed a high proportion of carbapenem-resistant strains in the wastewater, with similarities to clinical isolates. The findings suggest that inadequately treated hospital wastewater may contribute to the spread of clinically important carbapenem-resistant A. baumannii strains in Ibadan.
MICROBIOLOGY SPECTRUM
(2023)