Article
Infectious Diseases
Lara Serrano-Aguirre, Rodrigo Velasco-Bucheli, Begona Garcia-Alvarez, Ana Saborido, Miguel Arroyo, Isabel de la Mata
Summary: This study identified a novel N-acylhomoserine lactone acylase from Actinoplanes utahensis, which can hydrolyze and synthesize various natural compounds, showing potential as a quorum quenching agent. This enzyme inhibits quorum sensing processes and can efficiently synthesize AHLs, highlighting its potential for the development of new antimicrobials.
Article
Agricultural Engineering
Shamas Tabraiz, Burhan Shamurad, Evangelos Petropoulos, Marcos Quintela-Baluja, Alex Charlton, Jan Dolfing, Paul J. Sallis
Summary: The novel QQ strain Acinetobacter guillouiae ST01 showed high activity in reducing quorum sensing in a membrane bioreactor by decreasing AHL concentrations in the sludge and biofilm. It also significantly reduced polysaccharide and protein levels, leading to a decrease in fouling rates in the MBR. These results suggest that A. guillouiae ST01 is a promising candidate for biofouling control in wastewater treatment.
BIORESOURCE TECHNOLOGY
(2021)
Article
Environmental Sciences
I-Chieh Chien, Chiao-Yun Chu, Chien-Yu Huang
Summary: Membrane bioreactors (MBRs) are widely used in wastewater treatment due to their high-quality effluent production. However, biofilm formation on membrane surfaces remains a major limitation of this technique. Bacterial quorum quenching (QQ) has been proven successful in controlling biofouling in MBRs. This study isolated potential QQ bacteria and investigated their degradation rates against eight different AHLs. The results showed that four isolates exhibited consistent AHL removal capabilities, indicating their potential for mitigating biofilm formation on MBR membranes.
Article
Chemistry, Physical
Bartlomiej Surpeta, Michal Grulich, Andrea Palyzova, Helena Maresova, Jan Brezovsky
Summary: Growing concerns about microbial antibiotic resistance have led to extensive research on overcoming antibiotic resistance. This study evaluated the degradation capacity of three enzymes on signaling compounds for Gram-negative bacteria and clarified the molecular mechanisms underlying their quorum quenching (QQ) activity. The study also observed substrate-dependent differences in the catalytic actions of these enzymes due to the distinct structures of their acyl-binding cavities and the dynamics of their molecular gates.
Article
Biotechnology & Applied Microbiology
Shereen A. Murugayah, Gary B. Evans, Joel D. A. Tyndall, Monica L. Gerth
Summary: By site-directed mutagenesis, the substrate specificity of a glutaryl-7-aminocephalosporanic acid acylase was changed from having no activity against N-acyl homoserine lactones to having new activities. This approach may be useful for further engineering of 'quorum quenching' enzymes.
BIOTECHNOLOGY LETTERS
(2021)
Article
Environmental Sciences
You Mit Prohim, Roent Dune A. Cayetano, Parthiban Anburajan, Nguyen Tang Thau, Sungmi Kim, Hyun-Suk Oh
Summary: Biomethane recovered through anaerobic digestion (AD) is a renewable and cost-effective alternative energy source that can help address rising energy demands. This study investigates the role of quorum sensing (QS) in AD systems and finds that the addition of signaling molecules (AHLs) enhances methane yield and promotes microbial growth and cell aggregation, ultimately improving biomethane synthesis.
Article
Biotechnology & Applied Microbiology
Zhe Zhou, Xiaozhen Wu, Jiayi Li, Yiyin Zhang, Yaohua Huang, Wenping Zhang, Yu Shi, Junxia Wang, Shaohua Chen
Summary: Quorum sensing (QS) is a microbial communication mechanism that regulates virulence factor production in pathogens. Quorum quenching (QQ) can block QS-mediated microbial infections. This study identified a novel QQ strain, XN-36, which can effectively degrade AHL and reduce the disease severity caused by P. carotovorum in host plants.
BIOLOGICAL CONTROL
(2022)
Article
Biotechnology & Applied Microbiology
Sahana Vasudevan, Parthasarathy Srinivasan, Prasanna Neelakantan, John Bosco Balaguru Rayappan, Adline Princy Solomon
Summary: In this study, a novel photoluminescence-based assay using cysteamine-capped titanium oxide nanoparticles was developed for the rapid and sensitive diagnosis of microbial infections. The system showed a maximum relative response of 98% for AHLs produced by Pseudomonas aeruginosa, with a linear detection range of 10-160 nM in simulated host urine. The efficacy and specificity of the system in detecting AHLs produced by P. aeruginosa were confirmed in a temporal manner.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biology
Syeda Javariya Khalid, Quratul Ain, Sher Jamal Khan, Amna Jalil, Muhammad Faisal Siddiqui, Tahir Ahmad, Malik Badshah, Fazal Adnan
Summary: Finding novel biological strategies to mitigate bacterial biofilms is crucial for combating bacterial infections as biofilm-forming bacteria are highly resistant to antibiotics. This study investigated the potential of quorum quenching (QQ) enzymes, which interfere with the quorum sensing (QS) mechanism, to mitigate biofilms. Bacterial strains with QQ activity were identified and tested against multidrug-resistant Pseudomonas aeruginosa. The results showed that certain QQ strains were able to reduce biofilm formation by up to 60%. The presence of AHL-degrading enzymes was confirmed, providing insights into the mechanism of biofilm mitigation.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Microbiology
Nicole E. Smalley, Amy L. Schaefer, Kyle L. Asfahl, Crystal Perez, E. Peter Greenberg, Ajai A. Dandekar
Summary: In the bacterium Pseudomonas aeruginosa, quorum sensing (QS) can activate the expression of dozens to hundreds of genes. In this study, the researchers investigated how the size and content of the QS regulon can evolve during long-term growth of P. aeruginosa. They found that the QS regulon can undergo a reductive adaptation in response to continuous QS-dependent growth. These findings provide insights into the strain-to-strain variability in the size and content of the P. aeruginosa QS regulon.
Article
Microbiology
Nicole E. Smalley, Amy L. Schaefer, Kyle L. Asfahl, Crystal Perez, E. Peter Greenberg, Ajai A. Dandekar
Summary: This study investigates the evolution of the QS regulon in Pseudomonas aeruginosa during long-term growth when cells require some but not all the functions activated by QS. The research reveals that the QS-regulon in P. aeruginosa can undergo a reductive adaptation in response to continuous QS-dependent growth, providing insights into the variability in the size and content of the QS regulon in different strains.
Article
Immunology
Melanie Gonzales, Laure Plener, Jean Armengaud, Nicholas Armstrong, Eric Chabriere, David Daude
Summary: This study demonstrates the importance of Quorum sensing in Burkholderia thailandensis using an enzyme-based quorum quenching strategy. The disruption of quorum sensing greatly affects bacterial behavior, including motility, proteolytic activity, and antimicrobial molecule production. Furthermore, the study shows that quorum sensing disruption significantly enhances the bactericidal activity against fungi and yeast. These findings are of prime interest for understanding the virulence of Burkholderia species and developing alternative treatments.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Zhixuan Feng, Xin Lu, Congli Chen, Yang Huo, Dandan Zhou
Summary: Fungal bulking can be controlled by regulating intercellular communication between fungi and bacteria. In this study, three signal molecules related to Penicillium fungal bulking under low-pH stress were identified. The addition of these molecules successfully inhibited mycelial formation and improved the settleability of activated sludge. Transcriptome analysis revealed the regulation of genes related to signal transduction, mycelium synthesis, and metabolic pathways.
Article
Environmental Sciences
Xiyuan Xu, Shuai Wang, Chengxuan Li, Jingxi Li, Fenglei Gao, Li Zheng
Summary: Microplastics in the oceans are a new type of pollutant with complex biofilms. This study investigated the diversity and biological characteristics of quorum sensing bacteria in microplastic-associated biofilms, and found that these bacteria have strong biofilm-forming capacity and potential to exacerbate adverse environmental effects.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Review
Engineering, Environmental
Xueping Wang, Deshuang Yu, Guanghui Chen, Chengju Liu, Ao Xu, Zhihao Tang
Summary: This paper discusses the bacterial communication mechanism based on QS and the environmental factors affecting QS, compares QS inhibition strategies, and explores the role and prospects of QQ in long-term operation systems.
WATER ENVIRONMENT RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Ming-Hsu Liu, Yi-Jr Chen, Chia-Yin Lee
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2018)
Article
Microbiology
San-Chi Chang, Chia-Yin Lee
Article
Biochemistry & Molecular Biology
Nai-Chen Wang, Tzu-Ping Ko, Chia-Yin Lee
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2008)
Article
Agricultural Engineering
Ren-Bao Liaw, Mei-Ping Cheng, Ming-Che Wu, Chia-Yin Lee
BIORESOURCE TECHNOLOGY
(2010)
Correction
Biotechnology & Applied Microbiology
Yi-Jr Chen, Pei-Chien Tsai, Chun-Hua Hsu, Chia-Yin Lee
ENZYME AND MICROBIAL TECHNOLOGY
(2014)
Article
Biotechnology & Applied Microbiology
Yi-Jr Chen, Pei-Chien Tsai, Chun-Hua Hsu, Chia-Yin Lee
ENZYME AND MICROBIAL TECHNOLOGY
(2014)
Article
Biotechnology & Applied Microbiology
Yi-Jr Chen, Yan-Chia Huang, Chia-Yin Lee
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2014)
Article
Biochemical Research Methods
Wen-Han Yu, Shiun-Cheng Su, Chia-Yin Lee
JOURNAL OF MICROBIOLOGICAL METHODS
(2008)
Article
Biochemistry & Molecular Biology
I-Chen Peng, Kai-Yin Lo, Chun-Hua Hsu, Chia-Yin Lee
PROCESS BIOCHEMISTRY
(2012)
Article
Biochemistry & Molecular Biology
Hui-Ju Chen, Tzu-Ping Ko, Chia-Yin Lee, Nai-Chen Wang, Andrew H. -J. Wang
Article
Microbiology
Ru-Yin Huang, Chia-Yin Lee
CURRENT MICROBIOLOGY
(2020)
Article
Microbiology
San-Chi Chang, Chia-Yin Lee
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Immunology
Ru-Yin Huang, Chia-Yin Lee
MICROBIOLOGY AND IMMUNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Nai-Chen Wang, Chia-Yin Lee
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2007)