Article
Food Science & Technology
Donghyun Lee, Hunseok Choi, Seonghyeon Son, Jonghyun Bae, Jayun Joo, Dong Wook Kim, Eun Jin Kim
Summary: The expression of virulence genes in Vibrio cholerae is regulated by the ToxR regulon, which is triggered by environmental stimuli during infection in the human small intestine. This study investigates the induction of virulence gene expression under laboratory culture conditions through two point mutations in the toxT gene. The findings suggest that the regulation of virulence gene expression may be more complex than previously thought, involving factors beyond the ToxR regulon.
Review
Medicine, General & Internal
David A. Montero, Roberto M. Vidal, Juliana Velasco, Sergio George, Yalda Lucero, Leonardo A. Gomez, Leandro J. Carreno, Richard Garcia-Betancourt, Miguel O'Ryan
Summary: This review provides an overview of the causative agent of cholera, Vibrio cholerae, including its evolution, pathogenesis, and the immune response against it. It highlights the adaptability of V. cholerae and its virulence factors, as well as the importance of vaccination in controlling cholera outbreaks. The review also discusses current and future vaccine developments.
FRONTIERS IN MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Hunseok Choi, Seonghyeon Son, Donghyun Lee, Jonghyun Bae, Eunyoung Seo, Dong Wook Kim, Eun Jin Kim
Summary: The study constructed new genes that can trigger the expression of TCP and CT in Vibrio cholerae, and induce antibody responses against TCP in animal models. However, the cholera toxin produced by these strains is secreted into the culture medium. To potentially apply in oral cholera vaccines, the researchers constructed V. cholerae strains that can express intracellular CTB under the control of the toxT-139F allele.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Yong Yan, Li Zhan, Guoying Zhu, Junyan Zhang, Ping Li, Lixia Chen, Peiyan He, Jianyong Luo, Zhongwen Chen
Summary: A novel multiplex assay for rapid detection of Vibrio cholerae serogroup and cholera toxin toxigenicity was developed. The assay showed good diagnostic sensitivities and is suitable for rapid identification and screening of large specimens.
Article
Food Science & Technology
Fabian Rivera-Chavez, Bradley T. Meader, Sinan Akosman, Vuk Koprivica, John J. Mekalanos
Summary: Vibrio cholerae uses its toxin to cause cholera, but a study found that a compound called benzopyrimido-pyrrolo-oxazinedione (BPO-27) can inhibit the toxin-induced symptoms, protecting mice from diarrhea and death. BPO-27 also suppresses the replication and survival of V. cholerae in the gut.
Article
Chemistry, Medicinal
Fernando Calzada, Elihu Bautista, Sergio Hidalgo-Figueroa, Normand Garcia-Hernandez, Claudia Velazquez, Elizabeth Barbosa, Miguel Valdes, Jesus Ivan Solares-Pascasio
Summary: Incomptines A and B are sesquiterpene lactones with various biological activities. IB showed stronger antibacterial activity against Vibrio cholerae compared to IA, while IA had a more significant impact on the action of cholera toxin. IA also exhibited higher anti-diarrheal activity than IB and the positive control racercadotril.
Article
Public, Environmental & Occupational Health
Xiaolan Huang, Fei Xiao, Nan Jia, Chunrong Sun, Jin Fu, Zheng Xu, Xiaodai Cui, Hui Huang, Dong Qu, Juan Zhou, Yi Wang
Summary: The ongoing outbreak of monkeypox, caused by monkeypox virus (MPXV), requires a rapid and sensitive MPXV detection method. In this study, a diagnostic test called MPX-LAMP-LFB was developed based on loop-mediated isothermal amplification (LAMP) and nanoparticle-based lateral flow biosensor (LFB) for the diagnosis of MPXV infection. The MPX-LAMP-LFB assay can specifically detect MPXV strains without cross-reaction with non-MPXV pathogens and has a low sensitivity of 5 copies/µl of plasmid template and 12.5 copies/µl of pseudovirus in human blood samples. The entire process of the assay can be completed in about 1 hour, making it a useful tool for rapid and reliable diagnosis of MPXV infection.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Microbiology
Eizo Takahashi, Sadayuki Ochi, Tamaki Mizuno, Daichi Morita, Masatomo Morita, Makoto Ohnishi, Hemanta Koley, Moumita Dutta, Goutam Chowdhury, Asish K. Mukhopadhyay, Shanta Dutta, Shin-Ichi Miyoshi, Keinosuke Okamoto
Summary: Three NAG vibrio strains isolated from pond water in Kolkata, India were found to potentially possess virulence factors that can induce severe syndrome, indicating a possible risk of future epidemics caused by these virulent NAG vibrios.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Bolutife Fakoya, Karthik Hullahalli, Daniel H. F. Rubin, Deborah R. Leitner, Roma Chilengi, David A. Sack, Matthew K. Waldor
Summary: This study created next-generation cholera challenge strains and demonstrated their accuracy and safety in measuring immunity and vaccine effectiveness.
Article
Environmental Sciences
Xiaotian Han, Ting Zhao, Tian Yan, Rencheng Yu
Summary: A LAMP method coupled with a LFD was developed for detecting harmful algae Karenia mikimotoi, showing high sensitivity and specificity, indicating a promising approach for detecting and monitoring harmful algal blooms.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Microbiology
Arindam Naha, Jeffrey H. Withey, Piyali Mukherjee, Rudra Narayan Saha, Prosenjit Samanta, Amit Ghosh, Shin-Ichi Miyoshi, Shanta Dutta, Asish K. Mukhopadhyay
Summary: This study investigated the regulation mechanism of the cholera toxin promoter and found that the number of heptad repeats in the promoter influenced gene transcription activation. The occupation of distal repeats by H-NS may inhibit the transcriptional activation of the ctx genes.
FEMS MICROBIOLOGY LETTERS
(2022)
Article
Multidisciplinary Sciences
Yuta Mikami, Kazumasa Fuwa, Eriko Arima, Yasuo Suda, Itaru Yanagihara, Satoshi Ibara
Summary: The study evaluated the efficacy of the loop-mediated isothermal amplification (LAMP) assay for detecting Ureaplasma spp. in respiratory tract samples of preterm infants, showing similar sensitivity and specificity to quantitative real-time polymerase chain reaction.
Article
Biophysics
Tyler Hertenstein, Yisha Tang, Alexander S. Day, Jocelyn Reynolds, Patrick V. Viboolmate, Jeong-Yeol Yoon
Summary: A new miRNA detection method was developed using a low-cost device and a unique fluorescence detection technique. This method could detect 3 types of miRNA in a short period of time and had high sensitivity and accuracy.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Multidisciplinary Sciences
Aftab Nadeem, Raghavendra Nagampalli, Eric Toh, Athar Alam, Si Lhyam Myint, Thomas Heidler, Mitesh Dongre, Nikola Zlatkov, Hudson Pace, Fouzia Bano, Anders Sjostedt, Marta Bally, Bernt Eric Uhlin, Sun Nyunt Wai, Karina Persson
Summary: MakA protein is part of a gene cluster in Vibrio cholerae, along with other proteins forming a tripartite cytolytic toxin that is toxic to both red blood cells and human colon carcinoma cells. This previously unrecognized toxin may contribute to fitness and virulence potential in different host environments and organisms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biotechnology & Applied Microbiology
Xinggui Yang, Yue Wang, Ying Liu, Junfei Huang, Qinqin Tan, Xia Ying, Yong Hu, Shijun Li
Summary: Brucella abortus is a major pathogen causing abortion in cattle, but current diagnostic methods are limited. A novel detection method combining LAMP and LFIA technologies was developed, demonstrating rapid, sensitive, and specific detection capabilities, suitable for both basic laboratories and field applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Immunology
Yen-Hung Liu, Wataru Yamazaki, Yu-Tsung Huang, Chun-Hsing Liao, Wang-Hui Sheng, Po-Ren Hsueh
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2019)
Article
Veterinary Sciences
Thi Ngan Mai, Van Diep Nguyen, Wataru Yamazaki, Tamaki Okabayashi, Shuya Mitoma, Kosuke Notsu, Yuta Sakai, Ryoji Yamaguchi, Junzo Norimine, Satoshi Sekiguchi
BMC VETERINARY RESEARCH
(2018)
Article
Infectious Diseases
Wataru Yamazaki, Ryohei Makino, Konomu Nagao, Hirohisa Mekata, Kenji Tsukamoto
TRANSBOUNDARY AND EMERGING DISEASES
(2019)
Article
Biotechnology & Applied Microbiology
Islam Ibrahim Sabike, Wataru Yamazaki
JOURNAL OF FOOD PROTECTION
(2019)
Article
Biotechnology & Applied Microbiology
N. Yashiki, Y. Yamazaki, M. Subangkit, T. Okabayashi, W. Yamazaki, Y. Goto
LETTERS IN APPLIED MICROBIOLOGY
(2019)
Article
Environmental Sciences
Ryohei Makino, Yasuko Yamazaki, Konomu Nagao, Francis Victor Apego, Hirohisa Mekata, Wataru Yamazaki
FOOD AND ENVIRONMENTAL VIROLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Y. Yamazaki, U. Thongchankaew-Seo, K. Nagao, H. Mekata, W. Yamazaki
LETTERS IN APPLIED MICROBIOLOGY
(2020)
Article
Agriculture, Dairy & Animal Science
Thi Ngan Mai, Wataru Yamazaki, Thanh Phong Bui, Van Giap Nguyen, Thi My Le Huynh, Shuya Mitoma, Hala El Daous, Emmanuel Kabali, Junzo Norimine, Satoshi Sekiguchi
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2020)
Article
Virology
Wataru Yamazaki, Yasufumi Matsumura, Uraiwan Thongchankaew-Seo, Yasuko Yamazaki, Miki Nagao
Summary: The study has successfully developed a simple and rapid method for extracting RNA from saliva and conducting COVID-19 testing, which can be used in field settings to potentially reduce the risk of infection.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Infectious Diseases
Emmanuel Kabali, Girja Shanker Pandey, Musso Munyeme, Penjaninge Kapila, Andrew Nalishuwa Mukubesa, Joseph Ndebe, John Bwalya Muma, Charles Mubita, Walter Muleya, Elizabeth Muligisa Muonga, Shuya Mitoma, Bernard Mudenda Hang'ombe, Anuwat Wiratsudakul, Mai Thi Ngan, Eslam Elhanafy, Hala El Daous, Nguyen Thi Huyen, Wataru Yamazaki, Tamaki Okabayashi, Maiku Abe, Junzo Norimine, Satoshi Sekiguchi
Summary: This cross-sectional study in Zambia discovered the transmission of antimicrobial resistance among Escherichia coli and other enterobacteria isolated from healthy wildlife and livestock cohabiting in a game ranch near Lusaka. The study highlights the need for further investigation on the role of wildlife in the development and transmission of antimicrobial resistance, a global concern.
Article
Multidisciplinary Sciences
Yasuko Yamazaki, Uraiwan Thongchankaew-Seo, Wataru Yamazaki
Summary: The study investigated the presence of SARS-CoV-2 in cultivated oysters sold in Japan and evaluated the risk of food-borne transmission. The results indicated that consumption of oysters cultivated in coastal areas may not be a risk factor for SARS-CoV-2 outbreaks, suggesting that well-developed sewage treatment facilities contribute to preventing viral contamination.
Article
Veterinary Sciences
Mai Thi Ngan, Huynh Thi My Le, Vu Ngoc Dang, Trinh Thi Bich Ngoc, Le Van Phan, Nguyen Thi Hoa, Truong Quang Lam, Nguyen Thi Lan, Kosuke Notsu, Satoshi Sekiguchi, Yasuko Yamazaki, Wataru Yamazaki
Summary: The lack of practical testing methods for African swine fever (ASF) has hindered early detection and early containment of the pandemic. In this study, a rapid and sensitive point-of-care test (POCT) was developed to detect ASF using swine whole blood samples. The POCT enabled crude DNA to be extracted from swine whole blood samples within 10 min at low cost and with ease. Compared to conventional real-time PCR detection, the POCT showed slightly lower sensitivity but comparable diagnostic accuracy. It is expected that this POCT will facilitate early diagnosis and containment of ASF.
VETERINARY MEDICINE AND SCIENCE
(2023)
Article
Infectious Diseases
Yasufumi Matsumura, Wataru Yamazaki, Taro Noguchi, Masaki Yamamoto, Miki Nagao
Summary: Direct detection tests for SARS-CoV-2 in respiratory samples show promising potential for rapid diagnosis of COVID-19. However, there are large differences in the performance of different assays, and laboratories need to consider these characteristics before implementing them.
JOURNAL OF CLINICAL VIROLOGY PLUS
(2023)
Article
Veterinary Sciences
Angela Patricia B. Chua, Remil L. Galay, Tetsuya Tanaka, Wataru Yamazaki
VETERINARY SCIENCES
(2020)
Article
Veterinary Sciences
Konomu Nagao, Ryohei Makino, Francis Victor Apego, Hirohisa Mekata, Wataru Yamazaki
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2019)