Article
Microbiology
Yuchao Zhu, Yanan Xu, Lu Hong, Chunxue Zhou, Jia Chen
Summary: The study demonstrated that DNA immunization with TgGRA39 induced effective humoral and cellular immune responses in mice, providing protective immunity against Toxoplasma gondii infection.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Abdol Sattar Pagheh, Ahmad Daryani, Paria Alizadeh, Hadi Hassannia, Sonia M. Rodrigues Oliveira, Tohid Kazemi, Fatemeh Rezaei, Maria de Lourdes Pereira, Ehsan Ahmadpour
Summary: This study evaluated the immune responses induced by a pcGRA14 + pcROP13 DNA vaccine cocktail in BALB/c mice. The vaccine significantly increased the production of specific antibodies and cytokines against toxoplasmosis, providing effective protection against T. gondii infection.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2021)
Article
Immunology
Youbo Shi, Jianbing Li, Weili Yang, Jia Chen
Summary: This study demonstrates that TgGRA35, TgGRA42, and TgGRA43 are potential vaccine candidates against Toxoplasma gondii infection, and the three-gene DNA vaccine cocktail provides the strongest protection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Jixu Li, Eloiza May Galon, Huanping Guo, Mingming Liu, Yongchang Li, Shengwei Ji, Iqra Zafar, Yang Gao, Weiqing Zheng, Paul Franck Adjou Moumouni, Mohamed Abdo Rizk, Maria Agnes Tumwebaze, Byamukama Benedicto, Aaron Edmond Ringo, Tatsunori Masatani, Xuenan Xuan
Summary: The mutant strain based on gene disruption of dense granule protein 9 (gra9) reduced parasite replication, attenuated virulence in mice, and decreased cyst formation. Delta gra9 vaccination induced full immune responses with high levels of pro-inflammatory cytokines, maintaining T gondii-specific antibody levels, and providing complete protective immunity in mouse models. The vaccine proved to be immunogenic and potent, conferring 100% protection against acute and chronic T gondii challenges.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Iti Saraav, Luisa Cervantes-Barragan, Philipp Olias, Yong Fu, Qiuling Wang, Leran Wang, Yi Wang, Matthias Mack, Megan T. Baldridge, Thaddeus Stappenbeck, Marco Colonna, L. David Sibley
Summary: Oral infection with Toxoplasma gondii exacerbates damage to the colon caused by chemical irritant and impairs wound healing by suppressing stem cell regeneration. Enhanced tissue damage is due to inflammatory monocytes releasing inflammatory mediators.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Hong-chao Sun, Pu-ming Deng, Yuan Fu, Jin-hua Deng, Rong-hui Xie, Jing Huang, Meng Qi, Tuan-yuan Shi
Summary: In this study, PLGA nanoparticles were used as a delivery system for T. gondii GRA12 and GRA7 antigens. Mice immunized with PLGA+GRA12 showed higher levels of IgG antibodies, increased lymphocyte proliferation, and a predominant Th1 immune response. Immunization with PLGA+GRA12 vaccine resulted in significantly reduced parasite loads and longer survival times compared to other vaccinated groups. However, PLGA was ineffective in enhancing the immune response induced by T. gondii GRA7.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Food Science & Technology
Pablo-Jesus Marin-Garcia, Nuria Planas, Lola Llobat
Summary: This article reviews the detection methods, prevalence, and control measures of Toxoplasma gondii in the food industry. T. gondii is a common parasite that causes infection through food contamination, but standardized detection methods are currently lacking in the industry. The main detection methods include bioassays, cell culture, molecular and microscopic techniques, and serological methods. Due to the high prevalence of T. gondii in many food products, standardized detection methods and control measures are necessary.
Article
Immunology
Min Chen, Pei Yang, Zixuan Xin, Jiating Chen, Weihao Zou, Lijuan Zhou, Lili Yang, Jiao Peng, Hongjuan Peng
Summary: Toxoplasma gondii is a pathogen causing toxoplasmosis and has similarities with immunotherapy for cancers. The knockout of GRA5 gene in T. gondii resulted in an avirulent strain that stimulated immune responses. ME49 & UDelta;gra5 vaccination provided protection against T. gondii infection and breast cancer by boosting anti-tumor responses. The vaccine upregulated Th1 cytokines and tumor-infiltrating T cells, and increased the number of immune cells in the spleen.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Parasitology
Gihan M. Tawfeek, Iman F. Abou-El-Naga, Eman Mohamed Elsayed Hassan, Dina Sabry, Rasha Abdallah Meselhey, Salwa Sami Younis
Summary: In this study, exosomes isolated from T. gondii infected human hepatoblastoma cells were evaluated for their potential as an immunizing agent against murine chronic toxoplasmosis. The results showed that exosome-alum induced both humoral and mixed Th1/Th2 cellular immune responses, and significantly reduced the brain cyst burden after challenge infection.
Article
Immunology
Mohammad Mahdi Jafari, Zahra Azimzadeh Tabrizi, Mohammad Saaid Dayer, Nazanin Atieh Kazemi-Sefat, Mahshid Mohtashamifard, Rahimeh Mohseni, Atefeh Bagheri, Saeed Bahadory, Amir Karimipour-Saryazdi, Fatemeh Ghaffarifar
Summary: Toxoplasma gondii infection is associated with immune response, which can be used as prognosis markers. Immunotherapy has become a new approach for toxoplasmosis treatment.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Immunology
Keon-Woong Yoon, Ki-Back Chu, Hae-Ji Kang, Min-Ju Kim, Gi-Deok Eom, Su-Hwa Lee, Eun-Kyung Moon, Fu-Shi Quan
Summary: Intranasal immunization with T. gondii ROP4 rBVs induces better mucosal and systemic immunity, leading to enhanced protection against lethal challenge infection compared to oral immunization.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Parasitology
Jiaofeng Huang, Jiaolong Zheng, Bang Liu, Lingling Lu, Haicong Wu, Su Lin, Dongliang Li
Summary: This study found that Toxoplasma gondii infection is associated with higher mortality in the general population, and this association is related to age and sex. This suggests that Toxoplasma gondii infection may be an important public health issue.
PARASITES & VECTORS
(2022)
Article
Multidisciplinary Sciences
Syrian G. Sanchez, Emilie Bassot, Aude Cerutti, Hoa Mai Nguyen, Amel Aida, Nicolas Blanchard, Sebastien Besteiro
Summary: Toxoplasma gondii is responsible for toxoplasmosis, a disease that can be serious when contracted during pregnancy, but can also be a threat for immunocompromised individuals. The study finds that targeting the apicoplast in bradyzoites, the latent form of the parasite, leads to a loss of long-term viability, suggesting the apicoplast as a potential therapeutic target.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Parasitology
Jing Zhang, Zhenke Yang, Hangbin Ma, Xiaowei Tian, Jiani Li, Zihang Zhang, Longxin Yang, Mengchuang Feng, Xuefang Mei, Zhenchao Zhang, Xingrui Li, Shuai Wang
Summary: In this study, recombinant T. gondii Calreticulin (rTgCRT) was successfully expressed and used as a subunit vaccine against T. gondii infection in mice. The results showed that rTgCRT can induce strong cellular and humoral immune responses, providing protection against acute and chronic toxoplasmosis.
Article
Veterinary Sciences
Xiaowei Tian, Hanqi Sun, Meng Wang, Guangmin Wan, Tong Xie, Xuefang Mei, Zhenchao Zhang, Xiangrui Li, Shuai Wang
Summary: Toxoplasma gondii, a parasite infecting billions of people and livestock, poses a major threat to economies and human health. The study demonstrates that the TgPLP2 protein can induce immune responses and prolong the survival of infected mice, making it a potential candidate for T. gondii vaccines.
FRONTIERS IN VETERINARY SCIENCE
(2022)