Article
Biotechnology & Applied Microbiology
David Rodriguez-Temporal, Margarita Estreya Zvezdanova, Pablo Benedi, Mercedes Marin, Mario Blazquez-Sanchez, Maria Jesus Ruiz-Serrano, Patricia Munoz, Belen Rodriguez-Sanchez
Summary: This study evaluated the efficacy of the VITEK MS Plus system coupled with updated RUO and IVD databases for identifying Nocardia spp. and Mycobacterium spp. clinical isolates. The results showed that 95.6% of Nocardia spp. isolates were correctly identified at the species or complex level using the IVD mode, and 86.7% using the RUO mode. For non-tuberculous Mycobacterium clinical isolates, 90.6% were correctly identified in the IVD mode and 84.0% in the RUO mode, with no misidentifications detected. This system's robustness and accuracy make it suitable for routine identification of these microorganisms in clinical laboratories.
MICROBIAL BIOTECHNOLOGY
(2023)
Review
Microbiology
Rita M. Traxler, Melissa E. Bell, Brent Lasker, Brendan Headd, Wun-Ju Shieh, John R. McQuiston
Summary: This review provides an update on the Nocardia review published by Brown-Elliott et al. in 2006, covering the taxonomic expansion, current identification methods, and the influence of new technologies on diagnosis and treatment. The clinical manifestations, epidemiology, and geographic distribution of the disease are briefly discussed, and an additional section on actinomycotic mycetoma is included.
CLINICAL MICROBIOLOGY REVIEWS
(2022)
Article
Chemistry, Analytical
Xufang Hu, Zongping Wang, Haolin Chen, An Zhao, Nianrong Sun, Chunhui Deng
Summary: An advanced metabolic analysis tool based on highly porous metal oxides derived from metal-organic frameworks (MOFs) is developed in this study. The tool successfully differentiates and stages renal cell carcinoma (RCC), as well as identifies the size of tumors. This achievement shows promising prospects for designer matrix-based analytical tools in disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Article
Multidisciplinary Sciences
Noa Wolff, Michaela Hendling, Fabian Schroeder, Silvia Schoenthaler, Andreas F. Geiss, Branka Bedenic, Ivan Barisic
Summary: Antibiotic resistances have led to treatment failures at an alarming rate, prompting the urgent need for obtaining as much information as possible for effective medication.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Margarita Estreya Zvezdanova, Pilar Escribano, Jesus Guinea, Patricia Munoz, David Rodriguez-Temporal, Belen Rodriguez-Sanchez
Summary: The implementation of commercial kits for standardized fungal sample preparation and updated reference libraries enables the easy use of MALDI-TOF MS for routine identification of fungal species. Although further improvement in the databases is needed, this method allows for the correct identification of a significant number of fungal species at the species level.
Article
Biochemical Research Methods
Ari Chae, Gwanwon Lee, Dong-Yeun Koh, Cheol-Min Yang, Sungho Lee, Young-Kwan Kim
Summary: Carbon fiber (CNF), prepared from electrospun PAN fibers, shows high salt tolerance, sensitivity, and resolution for matrix-free LDI-MS analysis, making it a promising material for developing an efficient and universal platform for analyte analysis regardless of ionization modes.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xian-Na Wang, Bin Li
Summary: Gold nanoparticles/thiol-beta-cyclodextrin-functionalized TiO2 nanowires were prepared to enhance the performance of SALDI MS and MSI, showing superior detection sensitivity and repeatability.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Hung Su, Zong-Han Jiang, Shu-Fen Chiou, Jentaie Shiea, Deng-Chyang Wu, Sung-Pin Tseng, Shu-Huei Jain, Chung-Yu Chang, Po-Liang Lu
Summary: An analytical platform combining probe sampling, thermal desorption-electrospray ionization/mass spectrometry (TD-ESI/MS), and multivariate statistical analysis was developed to rapidly differentiate bacterial species based on the differences in their lipid profiles. The results were validated by protein fingerprinting using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF). The developed platform showed promising potential as a useful diagnostic tool for rapidly distinguishing bacterial species.
Article
Microbiology
Anil K. Persad, Hanan A. Fahmy, Nicholas Anderson, Jing Cui, Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M. Spanninger, Robert L. Buchanan, Kalmia E. Kniel, Michele T. Jay-Russell, Michelle D. Danyluk, Gireesh Rajashekara, Jeffrey T. LeJeune
Summary: This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of Salmonella serovars.
Article
Infectious Diseases
Jun Li, Hui Shen, Ting Yu, Xiao-Yan Tao, Yong-Mei Hu, Hai-Chen Wang, Ming-Xiang Zou
Summary: This study investigated the clinical features, distribution, and antimicrobial susceptibility of Nocardia species isolated from pulmonary nocardiosis cases in a tertiary hospital in China. The most frequently identified species was N. farcinica, and the top predisposing factors for pulmonary nocardiosis were chronic obstructive pulmonary disease, hypertension, and tuberculosis. The isolated Nocardia species showed susceptibility to amikacin, trimethoprim/sulfamethoxazole, and linezolid, but resistance was observed against certain antibiotics.
JAPANESE JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Chadaporn Chaiden, Janthima Jaresitthikunchai, Anusak Kerdsin, Nattakan Meekhanon, Sittiruk Roytrakul, Suphachai Nuanualsuwan
Summary: In this study, MALDI-TOF-MS analysis of extracted peptides was developed to improve the classification of S. suis serotypes. The method successfully differentiated highly pathogenic SS2 and SS14 from other serotypes, indicating its potential as an alternative approach for serotyping clinically pathogenic S. suis isolates.
Article
Biochemical Research Methods
Hamideh Hamidi, Ramin Bagheri Nejad, Ali Es-haghi, Alireza Ghassempour
Summary: In this study, matrix-assisted laser desorption/ionization-time-offlight mass spectrometry (MALDI-TOF MS) was used to distinguish Brucella abortus and Brucella melitensis. By analyzing the proteomic mass patterns of whole cells, specific biomarkers for each strain were identified. The combination of bioinformatics algorithms and mapping of ribosomal and structural proteins improved the accuracy of protein identification. This method could serve as a cost-effective strategy for diagnosing brucellosis and identifying dangerous bacterial species.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Ying Feng, Xianhu Wei, Moutong Chen, Honghui Zhu, Jumei Zhang, Youxiong Zhang, Liang Xue, Lanyan Huang, Guoyang Chen, Qihui Gu, Shi Wu, Yu Ding, Qingping Wu
Summary: This study developed a new deep semi-quantitative fingerprinting method using pseudotargeted metabolomics and deep learning techniques, achieving a prediction accuracy of Listeria monocytogenes serotypes exceeding 99%. This technology demonstrates great potential for the rapid and accurate identification of pathogens.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sangwon Cha, Gyouwoong Jun, Yougyeong Park, Sung Jun An, Donghoon Lee
Summary: Nanospray desorption electrospray ionization (nano-DESI) was used to analyze the metabolite fingerprints on Hypericum leaves. The intensity changes of key metabolites over time were observed through full mass scan experiments and monitored using selected ion monitoring and pseudo-selected reaction monitoring experiments. The correlation between the time-dependent changes in the metabolite fingerprints and depth-wise metabolite distribution was examined by removing the surface layers of the leaves multiple times.