Article
Biochemistry & Molecular Biology
Soochul Shin, Kwangbeom Hyun, Jinwoo Lee, Dongwon Joo, Tomasz Kulikowicz, Vilhelm A. Bohr, Jaehoon Kim, Sungchul Hohng
Summary: The oligomeric states of Werner syndrome protein (WRN) were determined using single-molecule multi-color fluorescence imaging. WRN binds to forked DNA as a dimer during unwinding and maintains its oligomeric state. However, it binds to replication fork as a tetramer and dimerizes during replication fork regression. The study reveals how the active dimers of WRN utilize ATP hydrolysis for repetitive unwinding and replication fork regression by selectively inhibiting the helicase activity on specific strands.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biology
Joseph A. Newman, Angeline E. Gavard, Simone Lieb, Madhwesh C. Ravichandran, Katja Hauer, Patrick Werni, Leonhard Geist, Jark Boettcher, John R. Engen, Klaus Rumpel, Matthias Samwer, Mark Petronczki, Opher Gileadi
Summary: Loss of WRN, a DNA repair helicase, is a strong vulnerability of MSI cancers and a promising drug target. ATP binding and hydrolysis are vital for genome integrity and viability of MSI cancer cells. The crystal structure of the WRN helicase core reveals unusual features which may play a role in the binding of alternative DNA structures, aiding in the development of WRN helicase inhibitors.
LIFE SCIENCE ALLIANCE
(2021)
Article
Biochemical Research Methods
Yanfang Chen, Severine Roselli, Nikita Panicker, Joshua S. Brzozowski, David A. Skerrett-Byrne, Heather C. Murray, Nicole M. Verrills
Summary: This article provides a comprehensive characterization of the proteome and phosphoproteome of wild-type primary MEFs from C57BL/6 mice. The study identifies abundant EIF2/4 and MTOR signaling pathways, as well as extracellular matrix and cytoskeleton associated pathways. Kinase enrichment analysis reveals the activation of P38A, P90RSK, P70S6K, and MTOR. This dataset serves as a valuable resource for future research in the field.
Article
Biochemistry & Molecular Biology
Leonardo Gatticchi, Maya Petricciuolo, Paolo Scarpelli, Lara Macchioni, Lanfranco Corazzi, Rita Roberti
Summary: Adipogenesis is regulated by a transcriptional cascade involving CEBP and PPAR family members and hormonally induced pathways. Tm7sf2 gene is involved in cholesterol biosynthesis and modulates the expression of the master gene of adipogenesis PPAR gamma, affecting adipose tissue homeostasis. Loss of Tm7sf2 impairs adipocyte differentiation by affecting adipogenic regulators and insulin signaling, indicating its importance in maintaining metabolic health.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Gintautas Vainorius, Maria Novatchkova, Georg Michlits, Juliane Christina Baar, Cecilia Raupach, Joonsun Lee, Ramesh Yelagandula, Marius Wernig, Ulrich Elling
Summary: In this study, the authors show that cells can take two mechanistically distinct paths in the same transition paradigm when driven by the similar proneural factors Ascl1 and Ngn2. Ascl1 dismantles the pluripotency network and installs neuronal and trophoblast cell fates, while Ngn2 generates a neural stem cell-like intermediate supported by incomplete shutdown of the pluripotency network.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Iyori Nojima, Ryusuke Hosoda, Yuki Toda, Yoshiki Saito, Naohiro Ueda, Kouhei Horimoto, Naotoshi Iwahara, Yoshiyuki Horio, Atsushi Kuno
Summary: Downregulation of IGFBP5 through activation of the ERK2 pathway is involved in replicative senescence in embryonic mouse fibroblasts.
Article
Biochemistry & Molecular Biology
Ryogo Katada, Junichi Tanaka, Koki Takamatsu, Kenji Hata, Rika Yasuhara, Shintaro Ohnuma, Ikuko Takakura, Riko Nishimura, Tatsuo Shirota, Kenji Mishima
Summary: Salivary gland hypofunction caused by radiation therapy or Sjogren syndrome can lead to various oral diseases and decrease in quality of life. Inducing salivary gland-like cells from transdifferentiated epithelial tissues provides a promising cell therapy strategy for treating dry mouth.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Romain O. Georges, Hugo Sepulveda, J. Carlos Angel, Eric Johnson, Susan Palomino, Roberta B. Nowak, Arshad Desai, Isaac F. Lopez-Moyado, Anjana Rao
Summary: The disruption of TET enzymes in mouse embryonic stem cells results in chromosome mis-segregation and aneuploidies, indicating the importance of TET enzymes and DNA methylation patterns in maintaining genome stability.
NATURE COMMUNICATIONS
(2022)
Article
Cell & Tissue Engineering
Imran Ullah, Yurianna Shin, Yeongji Kim, Keon Bong Oh, Seongsoo Hwang, Young-Im Kim, Jeong Woong Lee, Tai-Young Hur, Seunghoon Lee, Sun A. Ock
Summary: Activation of endogenic Hnf4a is crucial for generating successful sex-specific miHeps, with male-derived miHeps exhibiting enhanced hepatic features compared to female miHeps. Transcriptomes from male miHeps closely resembled those of liver, particularly in terms of Hnf4a expression.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Benjamin J. Richards, Jason A. Glab, George W. Mbogo, Darani Dahanayake, Brian J. Smith, Hamsa Puthalakath, Belinda M. Abbott
Summary: Chronic activation of beta-adrenergic receptors leads to cardiomyocyte apoptosis, and inhibiting Bim expression is a potential protective strategy.
BIOORGANIC CHEMISTRY
(2022)
Article
Endocrinology & Metabolism
Constanza Contreras-Jurado, Ana Montero-Pedrazuela, Raul F. Perez, Susana Alemany, Mario F. Fraga, Ana Aranda
Summary: This study found that triiodothyronine (T3), a thyroid hormone, enhances the reprogramming of mouse embryonic fibroblasts (MEFs) into induced pluripotent stem cells (iPSCs). T3-induced iPSCs show similar characteristics to embryonic stem cells and have the potential for differentiation into the three major germ layers. The inclusion of T3 in reprogramming strategies could be useful for studying cell plasticity, disease, and regenerative medicine.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Cell Biology
Kenly Wuputra, Ming-Ho Tsai, Kohsuke Kato, Ya-han Yang, Jia-Bin Pan, Chia-Chen Ku, Michiya Noguchi, Shotaro Kishikawa, Koji Nakade, Hua-Ling Chen, Chung-Jung Liu, Yukio Nakamura, Kung-Kai Kuo, Ying-Chu Lin, Te-Fu Chan, Deng-Chyang Wu, Ming-Feng Hou, Shau-Ku Huang, Chang-Shen Lin, Kazunari K. Yokoyama
Summary: The study found that DMSO can induce cell apoptosis by activating the AhR promoter and requires the involvement of Jdp2. Additionally, transcription factors such as AhR and Nrf2 form an activation complex with Jdp2 to enhance AhR promoter activity in response to DMSO.
CELL BIOLOGY AND TOXICOLOGY
(2022)
Article
Genetics & Heredity
Feng Wang, Ran Tao, Li Zhao, Xin-Hui Hao, Yi Zou, Qing Lin, Meng Meng Liu, Graham Goldman, Daoshu Luo, Shuo Chen
Summary: Bmp2 is crucial for dentin development, and its knock-out in mice leads to a DGI-like phenotype. The study identified 22 differentially expressed lncRNAs and 227 differentially expressed mRNAs in Bmp2 knockout cells, revealing their roles in cell differentiation and signaling pathways. Network analysis provided insights into how Bmp2 regulates coding and non-coding RNAs during dentinogenesis.
FRONTIERS IN GENETICS
(2021)
Article
Multidisciplinary Sciences
Hiroyuki Omori, Masahiro Otsu, Haruo Nogami, Masayoshi Shibata
Summary: Cells have a regulatory mechanism called heat shock (HS) response, which is sensitive to both exogenous and endogenous factors, leading to both positive and negative effects on cells. The response of neural stem cells to heat shock is unclear, but studies have shown that extending the pre-culture period significantly affects the heat shock response and cell survival.
Article
Biochemistry & Molecular Biology
Bong Jong Seo, Seung Bin Na, Joonhyuk Choi, Byeongyong Ahn, Omer Habib, Chankyu Park, Kwonho Hong, Jeong Tae Do
Summary: Deletion of the mitochondrial fission-related gene Dnml1 affects the differentiation ability of mouse embryonic stem cells (ESCs) and inhibits their pluripotency. This inhibition may be achieved through metabolic shift, G2/M phase arrest, and modulation of gene expression.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Oncology
Siker Kimbung, Ida Markholm, Judith Bjohle, Tobias Lekberg, Anna von Wachenfeldt, Edward Azavedo, Ariel Saracco, Mats Hellstrom, Srinivas Veerla, Eric Paquet, Par-Ola Bendahl, Marten Ferno, Jonas Bergh, Niklas Loman, Thomas Hatschek, Ingrid Hedenfalk
INTERNATIONAL JOURNAL OF CANCER
(2018)
Article
Biochemistry & Molecular Biology
Brandi Sharp, Eric Paquet, Felix Naef, Akanksha Bafna, Herman Wijnen
NUCLEIC ACIDS RESEARCH
(2017)
Article
Biochemistry & Molecular Biology
Guillaume Combes, Helena Barysz, Chantal Garand, Luciano Gama Braga, Ibrahim Alharbi, Philippe Thebault, Luc Murakami, Dominic P. Bryne, Stasa Stankovic, Patrick A. Eyers, Victor M. Bolanos-Garcia, William C. Earnshaw, John Maciejowski, Prasad V. Jallepalli, Sabine Elowe
Article
Biochemistry & Molecular Biology
Lucie Aumailley, Marie Julie Dubois, Tracy A. Brennan, Chantal Garand, Eric R. Paquet, Robert J. Pignolo, Andre Marette, Michel Lebel
Article
Biochemistry & Molecular Biology
Emmanuelle Gouot, Wajid Bhat, Anne Rufiange, Eric Fournier, Eric Paquet, Amine Nourani
NUCLEIC ACIDS RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Andrea Brigitta Alber, Eric Raphael Paquet, Martina Biserni, Felix Naef, David Michael Suter
Article
Biochemical Research Methods
Andrea Manfrin, Yoji Tabata, Eric R. Paquet, Ambroise R. Vuaridel, Francois R. Rivest, Felix Naef, Matthias P. Lutolf
Article
Physics, Multidisciplinary
Colas Droin, Eric R. Paquet, Felix Naef
Article
Cell Biology
Luciano Gama Braga, Diogjena Katerina Prifti, Chantal Garand, Pawan Kumar Saini, Sabine Elowe
Summary: Accurate chromosome alignment during metaphase is crucial for proper chromosome segregation in cell division, and tools to measure alignment errors are important for understanding chromosome segregation defects. This study developed and validated a method to quantify chromosome misalignment, which will allow sensitive and systematic analysis of chromosome segregation defects in mitotic cells.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Agriculture, Dairy & Animal Science
F. Huot, S. Claveau, A. Bunel, D. E. Santschi, R. Gervais, E. R. Paquet
Summary: Low reticuloruminal pH (rpH) in cows can lead to sub-acute ruminal acidosis (SARA), which can negatively impact rumen health and animal performance. Through an observational study on 110 early-lactation Holstein cows from 12 commercial farms, we found that rpH variability is influenced by farm management factors such as automatic milking system and presence of corn silage in the ration. The study also showed that rpH is highly variable among animals on the same farm.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Anais Chancy, Debora E. Santschi, Eric R. Paquet, David L. Renaud, Marie-Lou Gauthier, Edith Charbonneau, Nicolas Barbeau-Gregoire, Laura Van Driessche, Sebastien Buczinski
Summary: The objective of this study was to standardize a reliable and repeatable swabbing technique using ATP luminometry to assess the cleanliness of feeding equipment for preweaning calves. The results showed excellent reliability of the direct swabbing technique and a positive correlation between luminometer values and visual scores and bacterial culture results.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Cell Biology
Luciano Gama Braga, Angel F. Cisneros, Michelle M. Mathieu, Maxime Clerc, Pauline Garcia, Baptiste Lottin, Chantal Garand, Philippe Thebault, Christian R. Landry, Sabine Elowe
Correction
Oncology
E. R. Paquet, R. Lesurf, A. Tofigh, V. Dumeaux, M. T. Hallett
BREAST CANCER RESEARCH
(2017)
Article
Oncology
E. R. Paquet, R. Lesurf, A. Tofigh, V. Dumeaux, M. T. Hallett
BREAST CANCER RESEARCH
(2017)