Article
Biochemistry & Molecular Biology
Bo Yu, Helena T. A. van Tol, Christine H. Y. Oei, Tom A. E. Stout, Bernard A. J. Roelen
Summary: The study demonstrates that lysophosphatidic acid (LPA) can accelerate bovine blastocyst formation, leading to up-regulation of TAZ and TEAD4 expression, as well as overexpression of YAP. However, the LPA-induced increase in CDX2 expression was only significant in day 8 embryos.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Daisuke Mashiko, Zenki Ikeda, Mikiko Tokoro, Yu Hatano, Tatsuma Yao, Tetsuya J. Kobayashi, Noritaka Fukunaga, Yoshimasa Asada, Kazuo Yamagata
Summary: In order to improve the performance of assisted reproductive technology, it is important to find an indicator that can select embryos that will be born or be aborted. This study found that embryos that were asynchronous during the 4-8-cell stage were more likely to be aborted. The delayed nuclear translocation of YAP1 and compaction, as well as reduced number of inner cell mass, suggested abnormal differentiation in asynchronous embryos. The observation of asynchrony in human embryos further supported the idea of synchrony being a universal indicator across animal species.
SCIENTIFIC REPORTS
(2022)
Article
Reproductive Biology
Luis Aguila, Ricardo P. Nociti, Rafael Sampaio, Jacinthe Therrien, Flavio Meirelles, Ricardo N. Felmer, Lawrence C. Smith
Summary: Haploid embryos have been significant in understanding parental genome's role in development and biotechnology. Bovine haploid androgenetic embryos (hAE) show severe hindrance in reaching the blastocyst stage. The transcriptomic profile comparison between hAE and biparental embryos revealed imbalances in pluripotency pathways, especially the WNT signaling pathway. Disturbance in pluripotency and canonical WNT pathways were observed due to altered transcript levels of several genes. Exposure to a GSK3B inhibitor improved the ability of hAE to reach the blastocyst stage, highlighting the importance of the WNT pathway in the developmental outcome of bovine hAE.
BIOLOGY OF REPRODUCTION
(2023)
Article
Multidisciplinary Sciences
Nicolas Allegre, Sabine Chauveau, Cynthia Dennis, Yoan Renaud, Dimitri Meistermann, Lorena Valverde Estrella, Pierre Pouchin, Michel Cohen-Tannoudji, Laurent David, Claire Chazaud
Summary: The initiation and regulation of epiblast lineage specification during embryonic development remains unclear. This study shows that coordinated expression of pluripotency markers is crucial for epiblast identity, and that this process is triggered by NANOG activity in a stochastic manner. The findings suggest that these features are likely conserved in human embryos as well.
NATURE COMMUNICATIONS
(2022)
Article
Developmental Biology
Yan Shi, Bingjie Hu, Zizengchen Wang, Xiaotong Wu, Lei Luo, Shuang Li, Shaohua Wang, Kun Zhang, Huanan Wang
Summary: The effects of GATA3 and CDX2, trophectoderm-associated factors, on lineage specification during early embryonic development in cattle were studied. Using a cytosine base editor system, the effects of these factors on the expression of lineage specification marker genes were determined. Knockout of GATA3 or CDX2 did not affect the developmental potential of embryos, but GATA3 deletion downregulated NANOG expression in bovine blastocysts. Further analysis showed that GATA3 is required for NANOG in the trophectoderm of bovine blastocysts. Single blastocyst RNA-seq analysis revealed that GATA3 deletion disrupts the transcriptome in bovine blastocysts.
Article
Immunology
Xiangguo Wang, Chaolei Chen, Lijuan Wang, Yunze Su, Boyu Li, Longfei Xiao, Zili Lin, Xihui Sheng, Xiaolong Qi, Hemin Ni, Yong Guo
Summary: This study investigates the regulation processes of the IFN tau-IFNAR cascade between the embryo and uterine epithelial cells in ruminants. The results suggest that specific activation of embryonic IFNAR1 and endometrial IFNAR2 by embryonic IFN tau directs normal uterine preparation for bovine early implantation.
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2022)
Review
Agriculture, Dairy & Animal Science
Yulianri Rizki Yanza, Malgorzata Szumacher-Strabel, Anuraga Jayanegara, Andre Meiditama Kasenta, Min Gao, Haihao Huang, Amlan Kumar Patra, Ewelina Warzych, Adam Cieslak
Summary: This study conducted a meta-analysis on the impact of medium-chain fatty acids (MCFA) on methane production in ruminants, finding that with increasing doses of MCFA, methane production decreased linearly under in vitro conditions and tended to decrease quadratically under in vivo conditions. Different sources of MCFA also showed variations in their effects on methane production in ruminants.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Iwona Ziolkowska-Suchanek, Marta Podralska, Magdalena Zurawek, Joanna Laczmanska, Katarzyna Izykowska, Agnieszka Dzikiewicz-Krawczyk, Natalia Rozwadowska
Summary: The study showed that FAM13A silencing had a negative impact on proliferation, migration, and invasion activity in NSCLC cells under both normal and hypoxic conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Ewelina Tomecka, Wioletta Lech, Marzena Zychowicz, Anna Sarnowska, Magdalena Murzyn, Tomasz Oldak, Krystyna Domanska-Janik, Leonora Buzanska, Natalia Rozwadowska
Summary: The study found that 5% oxygen concentration had beneficial effects on the proliferation, clonogenicity, and senescence of hWJ-MSCs, while not influencing cell morphology, immunophenotype, or neuroprotective effect. The potential for differentiation into adipocytes, osteocytes, and chondrocytes was comparable under both oxygen conditions, with enhanced neuronal differentiation seen under atmospheric oxygen conditions. Mitochondrial respiration was more important for the cells regardless of oxygen conditions, suggesting that hWJ-MSCs can be effectively cultured under both oxygen conditions, but 5% oxygen level may provide a more balanced environment.
Article
Biochemistry & Molecular Biology
Jessica E. Forsyth, Ali H. Al-Anbaki, Roberto de la Fuente, Nikkinder Modare, Diego Perez-Cortes, Isabel Rivera, Rowena Seaton Kelly, Simon Cotter, Berenika Plusa
Summary: Architectural changes at the cellular and organism level are crucial for successful development, yet poorly defined during mammalian preimplantation. IVEN, an interactive pipeline, helps classify cells and quantify cell neighborhood changes, revealing important correlations with embryo geometry and major developmental events. Additionally, studies using IVEN show that modulation of the FGF pathway impacts cell spatial relations and neighborhood distributions, leading to embryo architecture changes.
Article
Genetics & Heredity
Paulina Lipinska, Ewa Sell-Kubiak, Piotr Pawlak, Zofia Eliza Madeja, Ewelina Warzych
Summary: The experiment aimed to investigate the effects of selective inhibition of glucose or fatty acid metabolism on in vitro maturation of bovine cumulus-oocyte complexes (COCs). The results showed that only glucose metabolism was crucial to oocyte development, as inhibition of glucose decreased embryo culture efficiency. Different responses to inhibition were observed in cumulus cells, with upregulation of ketone body metabolism suggesting unsuccessful attempts to switch to lipid consumption. Treatment with etomoxir did not affect embryo development, indicating undisturbed metabolism in cumulus cells.
Article
Cell Biology
Urszula Kozlowska, Aleksandra Klimczak, Karolina Anna Bednarowicz, Tomasz Zalewski, Natalia Rozwadowska, Katarzyna Chojnacka, Stefan Jurga, Eytan R. Barnea, Maciej K. Kurpisz
Summary: This study aimed to investigate the efficiency of glial restricted progenitors (GRPs) grafted into murine CNS in different immune environments, and to study the effects of various immunosuppressive/immunomodulatory regimens on their survival and immune response.
Article
Biochemistry & Molecular Biology
Magdalena Nowaczyk, Agnieszka Malcher, Agnieszka Zimna, Wojciech Labedz, Lukasz Kubaszewski, Wojciech Barczak, Blazej Rubis, Natalia Rozwadowska, Maciej Kurpisz
Summary: The study demonstrated that PBN conditioning of human SkMDS/PCs under hypoxic conditions has significant advantages, such as increased number of cells with specific phenotype markers, enhanced ability to form functional myotubes, and decreased cell apoptosis. Additionally, PBN conditioning significantly increased the expression levels of myogenic genes.
Article
Biochemistry & Molecular Biology
Zofia E. Madeja, Marta Podralska, Agnieszka Nadel, Marcin Pszczola, Piotr Pawlak, Natalia Rozwadowska
Summary: Mitochondrial content and activity can serve as reliable markers for evaluating bovine sperm quality, with motile sperm having more mitochondria and mtDNA copies, as well as higher mitochondrial membrane potential.
Article
Reproductive Biology
Dorota Lechniak, Ewa Sell-Kubiak, Ewelina Warzych
Summary: This study investigates the impact of the Primo Vision embryo culture system on embryo development and quality compared to the classical system, as well as the effect of first zygotic cleavage (FZC) dysmorphisms on the metabolomic profile of blastocysts. The results show that Primo Vision embryos have a higher cleavage rate but a reduced blastocyst rate. Metabolomic analysis reveals differences in the activity of five pathways between the Primo Vision and classical systems. Furthermore, the FZC pattern significantly affects the metabolite profiles of blastocysts.
Review
Biochemistry & Molecular Biology
Weronika Wargocka-Matuszewska, Witold Uhrynowski, Natalia Rozwadowska, Zbigniew Rogulski
Summary: Cardiovascular diseases (CVD) encompass a range of conditions affecting the heart and blood vessels, and effective treatment relies on understanding the causes and monitoring the progression of these disorders. Molecular imaging, particularly positron emission tomography (PET), offers non-invasive visualization and quantification of biological pathways at the molecular level, providing valuable insights into CVD development. The use of radioisotope markers has proven to be the best method for in vivo studies of CVD-related phenomena in both pre-clinical research and clinical studies. This review highlights recent advances, challenges, and future prospects of PET imaging with radioisotope markers in rodent models for studying CVD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Natalia Rozwadowska, Maria Sikorska, Katarzyna Bozyk, Klaudia Jarosinska, Anna Cieciuch, Sonia Brodowska, Marcin Andrzejczak, Maria Siemionow
Summary: This study aimed to evaluate different isolation strategies and culture media formulations for the optimization of human myoblasts culture. The results showed that isolation strategies and cell density had little impact on the proliferative potential of myoblasts, but low cell density maintained the expression of CD56 marker. Evaluation of different culture media indicated that DMEM-based media without CEE performed the best. This study is of importance for the optimization of myoblasts culture in vitro.
AMERICAN JOURNAL OF STEM CELLS
(2022)
Article
Cell & Tissue Engineering
Ekaterina Semenova, Mariusz P. Grudniak, Eugeniusz K. Machaj, Katarzyna Bocian, Magdalena Chroscinska-Krawczyk, Marzena Trochonowicz, Igor M. Stepaniec, Magdalena Murzyn, Karolina E. Zagorska, Dariusz Boruczkowski, Tomasz J. Kolanowski, Tomasz Oldak, Natalia Rozwadowska
Summary: The study compared MSCs from three different regions of the umbilical cord and found that WJ-MSCs have high and stable proliferation potential and phenotype, making them a preferable source for medical applications.
STEM CELL REVIEWS AND REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
Piotr Pawlak, Ewelina Warzych, Zofia E. Madeja, Tomasz Nowak, Dorota Lechniak
Summary: The study found that oocytes from bitches with CEH/P have higher mtDNA content but do not show a negative impact on oocyte quality as reflected by the two parameters examined.
ANNALS OF ANIMAL SCIENCE
(2021)