Article
Plant Sciences
Sachinthi S. Amarasiri, Anoja P. Attanayake, Lakmini K. B. Mudduwa, Kamani A. P. W. Jayatilaka
Summary: The study investigated the nephroprotective mechanisms of Ambrette leaf extracts against adriamycin-mediated acute kidney injury. The results showed that Ambrette exerted promising protective effects through antioxidant, anti-inflammatory, and anti-apoptosis pathways, making it a potential candidate for the development of therapeutic drug leads for the treatment of acute kidney injury.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Plant Sciences
Myuri Parusnath, Yougasphree Naidoo, Moganavelli Singh, Farzad Kianersi, Yaser Hassan Dewir
Summary: This study investigated the antioxidant and antibacterial activities of various extracts from Combretum molle, a medicinal plant used in African traditional medicine. The methanol extracts showed strong radical-scavenging activity and broad-spectrum antibiotic capabilities. These findings suggest that C. molle has potential as a source for developing new drugs.
Article
Biochemistry & Molecular Biology
Hee Young Kim, Meran Keshawa Ediriweera, Kyung-Hwan Boo, Chang Sook Kim, Somi Kim Cho
Summary: The study found that steaming and microwaving increased the total polyphenol content of broccoli florets, while steaming decreased the total flavonoid content and microwaving slightly increased it. All broccoli extracts demonstrated similar radical scavenging and cytoprotective effects. Steamed (3 min)-freeze-dried (F-S3) and microwaved (2 min)-freeze-dried (F-M2) samples showed elevated levels of L-sulforaphane and strong anti-proliferative effects in MCF-7 cells.
Article
Agriculture, Multidisciplinary
Wen Xia, Bingbing Song, Tong Li, Rui Hai Liu
Summary: This study aimed to investigate the synergistic effects of blueberry and apple peel extracts in inhibiting the proliferation of liver cancer cells. The results showed that both extracts were rich in phytochemicals and had potent antioxidant activities, and their combination synergistically inhibited cell proliferation. This study provides a theoretical basis for the synergistic effects of different phytochemicals in health care.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Plant Sciences
Juveria Samar, Ghazala Yasmeen Butt, Anis Ali Shah, Adnan Noor Shah, Sajad Ali, Basit Latief Jan, Nader R. Abdelsalam, Muhammad Hussaan
Summary: Seaweeds are a rich source of bioactive compounds with antimicrobial properties. Padina antillarum, a type of seaweed, contains various chemical components that can inhibit the growth of bacteria and fungi. Additionally, the methanolic extract of P. antillarum shows potential benefits in treating diabetes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Idin Sahidin, Wahyuni, Arief Rabbani Rahim, Muhammad Arba, Agung Wibawa Mahatva Yodha, Nur Syifa Rahmatika, Carla W. Sabandar, Marianti A. Manggau, Rozida Mohd Khalid, Laode M. R. Al Muqarrabun, Andi Rifki Rosandy, Agus Chahyadi, Rika Hartati, Elfahmi
Summary: This study investigated the chemical constituents and biological activities of Etlingera calophrys rhizome. The compounds isolated from the rhizome showed scavenging and antimicrobial activities against several bacteria. One compound also exhibited inhibition of xanthine oxidase activity in silico.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2022)
Article
Biochemistry & Molecular Biology
Mengqi Wu, Jingying Xu, Hui Zhang, Wei Xia, Wei Li, Wenqing Zhang
Summary: This study aims to recycle bioactive flavonoids from Rosa setate x Rosa rugosa waste biomass and develop high-value products. The purified extracts from the waste showed higher antioxidant activity, stronger antiproliferative activity against certain cell lines, and enhanced bacteriostatic effects compared to crude extracts. These flavonoid-enriched extracts have the potential to be used in functional food and pharmaceutical industries.
Article
Biochemistry & Molecular Biology
Kristina Bimbiraite-Surviliene, Mantas Stankevicius, Simona Sustauskaite, Agnieszka Gegotek, Audrius Maruska, Elzbieta Skrzydlewska, Zita Barsteigiene, Ieva Akuneca, Ona Ragazinskiene, Audronis Lukosius
Summary: The study compared the composition and resulting antioxidant and biological properties of summer savory at different growth stages collected in the botanical garden of Vytautas Magnus University in central Lithuania climatic conditions.
Article
Agriculture, Multidisciplinary
Yanfang Li, Zhihao Liu, Gillian Manka Tamia, Xiaohua He, Jianghao Sun, Pei Chen, Seong-Ho Lee, Thomas T. Y. Wang, Boyan Gao, Zhuohong Xie, Liangli Lucy Yu
Summary: Different types of phenolic compounds in tomato seeds were analyzed, with the insoluble bound fraction showing stronger antioxidant activity and anti-cancer cell proliferation effects. The soluble free and insoluble bound fractions significantly inhibited colorectal cancer cell proliferation, providing a basis for the future use of tomato seeds as nutraceuticals in dietary supplements and functional foods.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Simona Fabroni, Angela Trovato, Gabriele Ballistreri, Susanna Aurora Tortorelli, Paola Foti, Flora Valeria Romeo, Paolo Rapisarda
Summary: This study developed a new extract from almond hull, rich in phenolic and triterpenoid components, using solid-solvent extraction and a food-grade hydroalcoholic solvent. The extract can be used as a functional ingredient in food, pharma, and cosmetic sectors. Among the tested cultivars, the Sicilian variety 'Romana' showed the highest recovery rate of triterpenoids and hydroxycinnamic acids, making it suitable for nutraceutical supplements production.
Article
Plant Sciences
Agata Maria Pawlowska, Natalia Zurek, Ireneusz Kapusta, Marinella De Leo, Alessandra Braca
Summary: This study evaluated the phenolic constituents of loquat fruits and leaves from Tuscany, Italy and their in vitro antioxidant and chemopreventive activities. The results showed that loquat leaves had higher antioxidant and anticancer potential compared to the fruits, and contained multiple phenolic compounds.
Review
Food Science & Technology
Diego Morales
Summary: Oak extracts have various biological activities, including antioxidant, anti-inflammatory, antitumoral, and antimicrobial effects. Further investigation is needed for additional bioactive molecules and abilities testing, evaluation of advanced extraction techniques, and conducting more animal studies and clinical trials to validate the activities of these extracts when included in food matrices.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nuntouchaporn Hutachok, Pimpisid Koonyosying, Tanachai Pankasemsuk, Pongsak Angkasith, Chaiwat Chumpun, Suthat Fucharoen, Somdet Srichairatanakool
Summary: The study analyzed the chemical compositions in Arabica coffee bean extracts and evaluated antioxidant and iron-chelating activities. The findings suggest that phenolics, particularly chlorogenic acid, play a significant role in these activities. Different coffee extracts exhibited varying degrees of free-radical scavenging properties, with drip coffee showing the most significant effect.
Article
Environmental Sciences
Krishna Kanthi Gudimella, Gangaraju Gedda, P. Senthil Kumar, B. K. Babu, Bhaskar Yamajala, Battula Venkateswara Rao, Prabal Pratap Singh, Deepak Kumar, Ajit Sharma
Summary: Fluorescent carbon dots were prepared from Carica Papaya Leaves using the sand bath method and characterized for various properties. The CDs exhibited excellent biological activities in biomedical applications, suggesting their potential as therapeutic agents.
ENVIRONMENTAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yao Liu, Zhimei Zhong, Liangliang Bao, Fang Wen, Hehe Yang
Summary: In this study, chitooligosaccharides were acylated with nonpolar amino acids to obtain new derivatives. The structure and antioxidant activities of these derivatives were investigated. The results showed that the derivatives exhibited strong scavenging ability towards hydroxyl radicals and superoxide anions, with 2-AlaLCOS showing significant reducing ability. In addition, the water solubility of the derivatives was greatly improved compared to nonpolar amino acids.
CARBOHYDRATE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Saleem Yousuf Bhat, Sonal Bhandari, Pavitra Suresh Thacker, Mohammed Arifuddin, Insaf Ahmed Qureshi
Summary: In this study, a series of quinoline-based hybrids were synthesized, leading to the discovery of a novel inhibitor molecule (QYT-4h) with high potency against the Leishmania donovani MetAP1 enzyme. The compound QYT-4h showed competitive inhibition against LdMetAP1 and high selectivity.
CHEMICAL BIOLOGY & DRUG DESIGN
(2021)
Article
Biochemistry & Molecular Biology
Sk. Abdul Amin, Kalyan Ghosh, Samayaditya Singh, Insaf Ahmed Qureshi, Tarun Jha, Shovanlal Gayen
Summary: The COVID-19 pandemic poses a global threat to public health, with the novel coronavirus possibly never completely disappearing. Studying enzymes vital for viral replication can accelerate the design of broad-spectrum PLpro inhibitors against infectious SARS-CoV and SARS-CoV-2 in the future.
MOLECULAR DIVERSITY
(2022)
Article
Biochemistry & Molecular Biology
Asra Nasir Khan, Insaf Ahmed Qureshi, Umar Khalid Khan, Vladimir N. Uversky, Rizwan Hasan Khan
Summary: The study investigated the potential anti-amyloidogenic behavior of the antibiotic levofloxacin, showing its ability to inhibit amyloid formation in human lysozyme and prevent fibrillation. Levofloxacin was found to stabilize lysozyme in its native state, disrupt amyloid fibrils, and potentially serve as a therapeutic against non-systemic amyloidosis and neurodegenerative diseases.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Chemistry, Multidisciplinary
Bandigi Narsimulu, Rahila Qureshi, Pranay Jakkula, Sayanna Are, Insaf Ahmed Qureshi
Summary: The study focuses on cloning, purification, and characterization of Ribulose-5-phosphate epimerase (RPE) of Leishmania donovani, revealing unique structural features of the enzyme. Biophysical and structural approaches showed the dimeric state of the enzyme and variations in alpha helical content under different conditions, while molecular docking and dynamics simulations provided insights into substrate and ligand binding. The research suggests that understanding the structure of leishmanial RPE may lead to the development of effective antileishmanial drugs.
Review
Biochemistry & Molecular Biology
Mayank Saini, Sayanna Are, Insaf Ahmed Qureshi
Summary: Human parasites cause deadly diseases, but there is no effective vaccine. Vitamin B6 plays a crucial role in parasites and the enzyme PdxK can be a potential target for drug discovery.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ayodeji Amobonye, Samayaditya Singh, Koel Mukherjee, Chacko Jobichen, Insaf Ahmed Qureshi, Santhosh Pillai
Summary: This study predicted and analyzed the structural characteristics of forty-four fungal glutaminases using computational tools, and predicted their properties. The results showed that these enzymes have long amino acid sequences, are extracellular, have acidic pIs, hydrophilic, and highly stable. Docking and simulation studies validated the interactions and structural stability of the enzymes, providing a reference for future wet lab studies and the development of drugs and fungicides.
PROCESS BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Samayaditya Singh, Insaf Ahmed Qureshi
Summary: This study predicted the three-dimensional structure of Toxoplasma gondii chorismate synthase (TgCS) using comparative modeling and investigated its binding mechanism through molecular docking and molecular dynamics simulations. Structure-based virtual screening identified EPSP analogs with higher binding affinity, providing a basis for the design and development of next generation chemotherapeutic molecules against toxoplasmosis.
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2022)
Article
Chemistry, Physical
Sk Abdul Amin, Janish Kumar, Samima Khatun, Sanjib Das, Insaf Ahmed Qureshi, Tarun Jha, Shovanlal Gayen
Summary: This study utilizes quantitative activity-activity relationship (QAAR) study to construct a mathematical framework for understanding the selectivity of HDAC8 over other HDACs. It also involves the screening of HDAC8 selectivity and toxicity assessment of in-house molecules, as well as the exploration of the binding mechanism between in-house compounds and HDAC8 using molecular dynamic simulation.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Biochemistry & Molecular Biology
Bandigi Narsimulu, Rahila Qureshi, Pranay Jakkula, Priti Singh, Mohammed Arifuddin, Insaf Ahmed Qureshi
Summary: In this study, the authors purified and characterized seryl tRNA synthetase (LdSerRS) and its mutants in Leishmania donovani. The purified LdSerRS showed high enzymatic activity and a higher content of alpha helices in secondary structure. A mutation in the ATP binding residues resulted in a reduced affinity for cofactor without significant alteration in secondary structure. The researchers identified Comp 5l as a specific inhibitor for leishmanial SerRS, which showed low potency towards purified HsSerRS. Structural analysis revealed the binding of Comp 5l into the cofactor binding site of LdSerRS with high affinity. The study provides a promising approach for developing specific therapeutics targeting leishmanial SerRS.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Chemistry, Multidisciplinary
Fouzia Nasim, Insaf Ahmed Qureshi
Summary: The ensemble of aminoacyl tRNA synthetases is a crucial component of the protein translation machinery and is considered an important target for the development of antimicrobial drugs. Studies on tRNA synthetase-ligand complexes have provided a detailed understanding of these enzymes, which play a critical role in decoding the genetic code. In addition to their role in antimicrobial drug development, aminoacyl tRNA synthetases have also been validated as important for the survivability of trypanosomatids, leading to the search for inhibitors that specifically target these enzymes.
Article
Biochemistry & Molecular Biology
Sourav Sardar, Jyotisha, Sk. Abdul Amin, Samima Khatun, Insaf Ahmed Qureshi, Umesh Kumar Patil, Tarun Jha, Shovanlal Gayen
Summary: HDAC1 is an important target for cancer epigenetics and different molecular modeling techniques have been used to study its interactions with natural inhibitors. By analyzing the molecular structures of these inhibitors, certain amino acid residues have been identified as potential binding sites. The stability of the HDAC1-natural inhibitors complexes has also been evaluated.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Bandigi Narsimulu, Pranay Jakkula, Rahila Qureshi, Fouzia Nasim, Insaf Ahmed Qureshi
Summary: This study explores the functional and structural aspects of Leishmania donovani glutamyl-tRNA synthetase (LdGluRS), an essential component in protein synthesis and a potential therapeutic target against parasitic diseases. The study demonstrates the competitive inhibition of LdGluRS by salicylate, which has negligible effect on human macrophages. The role of histidine as a catalytic residue is also highlighted. The findings provide insights into the molecular inhibition and structural features of glutamyl-tRNA synthetase from kinetoplastid parasites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Immunology
Jyotisha, Rahila Qureshi, Insaf Ahmed Qureshi
Summary: This study aims to design and evaluate a multi-epitope vaccine against Leishmaniasis. The designed vaccine was highly antigenic, non-allergenic, and non-toxic, with suitable physicochemical properties. Molecular docking and simulation studies confirmed the high binding affinity and stable interactions between human toll-like receptors and the vaccine. In addition, the vaccine was codon optimized, cloned, and expressed in a bacterial host, showing promising potential as a vaccine candidate against Leishmania parasites.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Forestry
Potladhurthi Nagendra, Lepakshi Mahmmad Bhakshu, Golla Narasimha, Annam Anjan Kumar
Summary: The present research aims to study the antioxidant, antimicrobial, and phytochemical properties of Vernonia albicans, a plant belonging to the Asteraceae family. The plant extracts showed significant antimicrobial activity against both Gram-positive and Gram-negative bacteria, suggesting their potential use in treating bacterial diseases. The study also analyzed the secondary metabolites present in the plant extracts.
Article
Biochemistry & Molecular Biology
Jyotisha, Samayaditya Singh, Insaf Ahmed Qureshi
Summary: COVID-19, caused by SARS-CoV-2, has resulted in significant global deaths, emphasizing the urgent need for vaccine development. The use of immunoinformatics to design a multi-epitope vaccine may provide specific immune responses against SARS-CoV-2.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)