Article
Biochemistry & Molecular Biology
Tatyana Grigoreva, Aleksandra Sagaidak, Angelina Romanova, Daria Novikova, Aleksander Garabadzhiu, Viacheslav Tribulovich
Summary: The development of chemoresistance in colorectal cancer cells was replicated in vitro by gradually increasing the drug content in the medium, resulting in reduced sensitivity to drugs of different mechanisms of action in resistant cell lines. The cells were found to utilize a universal efflux defense mechanism, which could be partially neutralized by inhibitors of ABC transport proteins, such as P-glycoprotein, confirming its role in chemoresistance.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Oncology
Ken Ito, Hirotaka Ishida, Fumiyoshi Fujishima, Yasuhiro Nakamura, Takuro Konno, Kazue Ise, Shuko Hata, Yohei Ozawa, Yusuke Taniyama, Takashi Kamei, Hironobu Sasano
Summary: This study found that the combination of nutlin-3 and chemotherapeutic agents may be a novel therapeutic strategy for esophageal cancer over-expressing MDM2, potentially leading to anti-tumor effects.
ANTICANCER RESEARCH
(2022)
Article
Medicine, Research & Experimental
Ru-Chen Xu, Fu Wang, Jia-Lei Sun, Weinire Abuduwaili, Guang-Cong Zhang, Zhi-Yong Liu, Tao-Tao Liu, Ling Dong, Xi-Zhong Shen, Ji-Min Zhu
Summary: In this study, a novel genetic cHCC-ICC mouse model was generated, providing insights into the molecular features and related signaling pathways of cHCC-ICC and identifying LAMB1 as a potential therapeutic target.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Katja Piaszinski, Martina Rincic, Thomas Liehr, Shaymaa Azawi
Summary: The study revealed genetic rearrangements in melanoma cell lines, involving oncogenes and tumor suppressor genes associated with aggressive forms of melanoma in humans. These cell lines can be considered as model systems for advanced eye and skin melanoma, aiding research into the molecular mechanisms and potential treatments for melanoma.
CYTOGENETIC AND GENOME RESEARCH
(2021)
Article
Oncology
Paulo Roberto Del Valle, Samir Andrade Mendonca, Fernanda Antunes, Aline Hunger, Rodrigo E. Tamura, Daniela Bertolini Zanatta, Bryan E. Strauss
Summary: A modified non-replicating adenoviral vector was developed for gene transfer in colorectal cancer cells, showing enhanced sensitivity to chemotherapy and increased cell death rates. The approach may be effective for the treatment of colorectal cancer, allowing for the use of limited drug doses.
CANCER BIOLOGY & THERAPY
(2021)
Article
Medicine, Research & Experimental
Andrea J. Manrique-Rincon, Luciana P. Ruas, Carolinne T. Fogagnolo, Randall J. Brenneman, Alexey Berezhnoy, Bianca Castelucci, Silvio R. Consonni, Eli Gilboa, Marcio C. Bajgelman
Summary: This study presents a novel strategy focusing on inhibiting regulatory T cells by using a chimeric aptamer targeting an immune activation marker and silencing Foxp3 gene, ultimately enhancing antitumor immune responses.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Review
Oncology
Nayara Gusmao Tessarollo, Ana Carolina M. Domingues, Fernanda Antunes, Jean Carlos dos Santos da Luz, Otavio Augusto Rodrigues, Otto Luiz Dutra Cerqueira, Bryan E. Strauss
Summary: Improvements in the design of nonreplicating adenoviral vectors, such as increasing specificity for target cells and reducing off-target effects, may provide new opportunities for cancer gene therapy and immunotherapy.
Article
Immunology
Luis A. Peroni, Jessica M. Toscaro, Camila Canateli, Celisa C. C. Tonoli, Renata R. de Olivera, Celso E. Benedetti, Lais D. Coimbra, Alexandre Borin Pereira, Rafael E. Marques, Jose L. Proenca-Modena, Gabriel C. Lima, Renata Viana, Jessica B. Borges, Hui Tzu Lin-Wang, Cely S. Abboud, Carlos Gun, Kleber G. Franchini, Marcio C. Bajgelman
Summary: Serological testing is a powerful tool in epidemiological studies for understanding viral circulation and assessing the effectiveness of virus control measures. Evaluation of viral antibody titers and changes in IgG levels are indicative of virus exposure and circulation reduction. High levels of antiviral IgG antibodies to different antigen targets may be associated with greater neutralization activity, enhanced by the presence of antiviral IgM antibodies.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Cell Biology
Daniele E. Mascarelli, Rhubia S. M. Rosa, Jessica M. Toscaro, Isadora F. Semionatto, Luciana P. Ruas, Carolinne T. Fogagnolo, Gabriel C. Lima, Marcio C. Bajgelman
Summary: Immunotherapy employs strategies to boost the host immune system's ability to detect and eliminate cancer cells, with costimulatory molecules playing a crucial role in the antitumor immune response.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Immunology
Jean Carlos dos Santos da Luz, Fernanda Antunes, Maria Alejandra Clavijo-Salomon, Emanuela Signori, Nayara Gusmao Tessarollo, Bryan E. Strauss
Summary: Reversible electropermeabilization is a technique that transiently increases membrane permeability by applying electrical pulses, widely used in various fields from medicine to food processing. In oncology, it is applied for intracellular transport of chemotherapeutic drugs and delivery of genetic material, serving as important cancer therapies.
Article
Neurosciences
Rodrigo S. Carraro, Guilherme A. Nogueira, Davi Sidarta-Oliveira, Rodrigo S. Gaspar, Nathalia R. Dragano, Joseane Morari, Vanessa C. D. Bobbo, Eliana P. Araujo, Natalia F. Mendes, Ariane M. Zanesco, Natalia Tobar, Celso D. Ramos, Jessica M. Toscaro, Marcio C. Bajgelman, Licio A. Velloso
Summary: NHLH2 is a hypothalamic transcription factor that plays a role in energy homeostasis and obesity. Overexpression of NHLH2 in mice can prevent and reduce obesity by affecting caloric intake.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Virology
Silvio R. Consonni, Anna C. P. V. de Carvalho, Artur B. Mauro, Kleber G. Franchini, Marcio C. Bajgelman
Summary: Preserving morphological features that are important for cell function and structure is crucial in in vitro experiments with rat cardiomyocytes. Lentiviral vectors are commonly utilized due to their flexibility, efficiency, and versatility in transducing quiescent cells. The combination of optimized promoters and heterologous envelopes resulted in robust expression and high efficiency transduction of rat ventricular myocytes without compromising ultrastructural features.
Article
Polymer Science
Thamires Santos Silva, Marcelo Melo Soares, Ana Claudia Oliveira Carreira, Gustavo de Sa Schiavo Matias, Carolina Coming Tegon, Marcos Massi, Andressa de Aguiar Oliveira, Leandro Norberto da Silva Junior, Hianka Jasmyne Costa de Carvalho, Gustavo Henrique Dona Rodrigues Almeida, Michelle Silva Araujo, Paula Fratini, Maria Angelica Miglino
Summary: Carbon nanostructures like graphene and graphene oxide have shown promising results in biomedical applications. The combination of graphene oxide with Poly L-lactic acid in PLLA-GO filaments demonstrated effective integration and absence of inflammation after 21 days of implantation in vivo. Further analysis is needed to evaluate their proliferative capacity and viability for tissue engineering applications.
Article
Oncology
Soledad Palameta, Andrea J. Manrique-Rincon, Jessica M. Toscaro, Isadora F. Semionatto, Matheus C. Fonseca, Rhubia S. M. Rosa, Luciana P. Ruas, Paulo S. L. Oliveira, Marcio C. Bajgelman
Summary: Therapeutic strategies based on immunomodulation have improved cancer therapy. A novel platform using virus-like particles derived from lentiviral capsids has been proposed to deliver targeted immunomodulatory signaling, enhancing antitumor response. The platform can be engineered to possess multifunctional ligands on the surface, driving tropism to the tumor site and delivering immunomodulatory signaling.
MOLECULAR THERAPY-ONCOLYTICS
(2022)
Article
Virology
Hui T. Lin-Wang, Rogerio C. Lemes, Eduardo da Silva Farias, Marcio C. Bajgelman, Kleber G. Franchini, Renata Viana, Carlos Gun
Summary: This study assessed the effectiveness of two COVID-19 vaccines, CoronaVac and AZ, in healthcare workers. The AZ vaccine showed a higher seroconversion rate and more common side effects. One dose of COVID-19 vaccine could produce an effective immune response in convalescent individuals.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Biology
A. C. Batissoco, K. Lezirovitz, D. B. Zanatta, C. R. M. L. Hemza, L. R. Vasques, B. E. Strauss, R. C. Mingroni-Netto, L. A. Haddad, R. F. Bento, J. Oiticica
Summary: The inhibition of Hes1 gene expression leads to an increase in hair cell number and upregulation of Myo7a and Sox2 mRNA levels in the organ of Corti, consistent with interference in the lateral inhibition mechanism in the inner ear. Lentivirus-mediated Hes1-shRNA expression is a valuable approach for genetic interference in the organ of Corti and future evaluation of hair cell regeneration in vivo.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Denise C. Fernandes, Joao Wosniak Jr, Renata C. Goncalves, Leonardo Y. Tanaka, Carolina G. Fernandes, Daniela B. Zanatta, Ana Barbosa M. de Mattos, Bryan E. Strauss, Francisco R. M. Laurindo
Summary: PDIA1 regulates the balance between NOX1 and NOX4, influencing VSMC differentiation and playing a significant role in vascular smooth muscle cell phenotype.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)