Article
Engineering, Biomedical
Isamu Ogawa, Daichi Onozato, Shiho Anno, Hisato Hayashi, Tatsuro Kanaki, Takahiro Iwao, Tamihide Matsunaga
Summary: This study proposes a new culture method for human induced pluripotent stem cell-derived intestinal organoids (HIOs) using low concentrations of natural polysaccharides in a suspension culture. The HIOs cultured in this method showed similarities to living intestines and exhibited pharmacokinetic functions. This method can be widely applied to drug discovery, disease research, and regenerative medicine, overcoming challenges in traditional culture methods.
Article
Genetics & Heredity
Huajin Li, Wenting Xu, Sijia Xiang, Leiting Tao, Wen Fu, Jinhui Liu, Wenbin Liu, Yamei Xiao, Liangyue Peng
Summary: Pluripotency is a transient state in early embryos regulated by a network of pluripotency-related genes. This study identified four pluripotent genes in zebrafish and crucian carp and verified that three of them can be used as markers for pluripotency in induced pluripotent stem cells in fish.
FRONTIERS IN GENETICS
(2022)
Article
Engineering, Biomedical
Yanxi Liu, Shraddha Chakraborty, Chatrawee Direksilp, Johannes M. Scheiger, Anna A. Popova, Pavel A. Levkin
Summary: The study demonstrates the feasibility of culturing hiPSCs in 200 nL droplets on DMA, showing that they can attach to the MG-free surface and maintain good viability without spontaneous differentiation. Additionally, culturing hiPSCs in 200 nL droplets leads to higher expression of the pluripotency marker Nanog compared to 2 mL culture.
MATERIALS TODAY BIO
(2021)
Article
Engineering, Biomedical
Debbie L. L. Ho, Stacey Lee, Jianyi Du, Jonathan D. D. Weiss, Tony Tam, Soham Sinha, Danielle Klinger, Sean Devine, Art Hamfeldt, Hope T. T. Leng, Jessica E. E. Herrmann, Mengdi He, Lee G. G. Fradkin, Tze Kai Tan, David Standish, Peter Tomasello, Donald Traul, Noushin Dianat, Rukmini Ladi, Quentin Vicard, Kishore Katikireddy, Mark A. A. Skylar-Scott
Summary: By combining sustainable culture of billions of human cells and bioprinting of cellular bioinks, this study demonstrates an approach to tissue engineering at organ-scale. The research optimized the suspension culture and successfully scaled it up, while maintaining pluripotency, and then used 3D bioprinting to differentiate the pluripotent aggregates into different cell lines.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Zhiyao Ma, Marcelo Augusto Szymanskide Toledo, Paul Wanek, Mohamed H. Elsafi Mabrouk, Francis Smet, Rock Pulak, Simon Pieske, Tobias Piotrowski, Werner Herfs, Christian Brecher, Robert H. Schmitt, Wolfgang Wagner, Martin Zenke
Summary: This article presents an automated platform for efficient and robust cultivation and differentiation of iPS cells into blood cells. By implementing cell cluster sorting and controlling the size of embryoid bodies (EBs), efficient differentiation of iPS cells can be achieved.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Polymer Science
Jin Hao, Ying Chen, Mingjian Zhu, Yingqing Zhao, Kai Zhang, Xia Xu
Summary: One approach to cell expansion is to use large hydrogel for growing a large number of cells. Nanofibrillar cellulose (NFC) hydrogel has been used for human induced pluripotent stem cell (hiPSCs) expansion. This study aims to understand the heterogeneity of hiPSCs at the single-cell level inside large NFC hydrogel during culture, especially in terms of spatial-temporal variations in biochemical composition. The results suggest that factors such as concentration gradients of growth factors and pH changes contribute to the heterogeneity observed in protein structure, glycosylation, and pluripotency of cells cultured in NFC hydrogel.
Article
Biotechnology & Applied Microbiology
Kazunori Bando, Hiromi Yamashita, Motomu Tsumori, Hayase Minoura, Koji Okumura, Fumiyuki Hattori
Summary: This study developed an all-in-one automated culture machine that can efficiently expand human pluripotent stem cells and differentiate them into various cell types. This would facilitate the social implementation of human pluripotent stem cells and contribute to the standardization of the entire handling processes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Rowan M. Karvas, Laurent David, Thorold W. Theunissen
Summary: Trophoblasts play critical roles in blastocyst implantation and maternal-fetal communication during pregnancy. However, our understanding of human trophoblast biology is limited due to the scarcity of first-trimester placental tissue and the inadequacy of animal models. Recent advancements in deriving human trophoblast stem cells from pluripotent and somatic cell sources provide exciting opportunities to study the molecular mechanisms of placental development and pregnancy-related diseases.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Engineering, Biomedical
Po-Hsiang Chang, Hsiao-Mei Chao, Edward Chern, Shan-hui Hsu
Summary: Chitosan membranes have been shown to sustain the proliferation and pluripotency of hiPSCs, promoting their self-assembly into 3D spheroids and direct differentiation into lineage-specific cells from the three germ layers. This convenient biomaterial-based culture system may facilitate the expansion and accessibility of hiPSCs for various applications in regenerative medicine, disease modeling, and drug screening.
Review
Cell Biology
Lay Shuen Tan, Juin Ting Chen, Lillian Yuxian Lim, Adrian Kee Keong Teo
Summary: This review discusses the key processes and guidelines for the clinical translation of human induced pluripotent stem cell (hiPSC)-derived beta cells for diabetes cell therapy, including the considerations for manufacturing clinical-grade hiPSCs, scaling up and differentiating clinical-grade hiPSCs into beta cells under clinically compliant conditions, and the mandatory quality control and product release criteria required by regulatory bodies for approving the use of cell-based products.
CELL PROLIFERATION
(2022)
Article
Biochemistry & Molecular Biology
Jiangtao Ren, Xuhua Zhang, Zhenhui Zhang, Jiafeng Pan, Zhexue Hao, Jin Li, Jun Liu
Summary: The complex and costly manufacturing process of CAR-T cell therapy has hindered its widespread adoption. Induced pluripotent stem cells (iPSCs) have potential as an alternative cellular immunotherapy due to their unlimited self-renewal potential and ability to differentiate into functional immune cells. Careful selection of the original cell for iPSC seed preparation is crucial due to the presence of epigenetic imprint. Our research shows that lymphocyte-origin cells are superior to embryonic stem cells in differentiating into functional immune cells. Blocking Fas-FasL induced apoptosis in T cells significantly improves iPSC generation without affecting their differentiation potential.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Canbin Zheng, Yingying Hu, Masahiro Sakurai, Carlos A. Pinzon-Arteaga, Jie Li, Yulei Wei, Daiji Okamura, Benjamin Ravaux, Haley Rose Barlow, Leqian Yu, Hai-Xi Sun, Elizabeth H. Chen, Ying Gu, Jun Wu
Summary: Cell competition is a fitness-sensing process where fitter cells eliminate less-fit but viable cells. The study developed an interspecies PSC co-culture strategy and identified a previously unknown mode of cell competition between species. Genetic inactivation of key components of the NF-kappa B complex in human cells improved the survival and chimerism of human cells in early mouse embryos.
Article
Biochemistry & Molecular Biology
Sergi Aranda, Anna Alcaine-Colet, Cecilia Ballare, Enrique Blanco, Ivano Mocavini, Aleksandra Sparavier, Pedro Vizan, Eva Borras, Eduard Sabido, Luciano Di Croce
Summary: The study found that cell cycle progression in pluripotent cells is influenced by variations in the response to differentiation cues, with the involvement of unknown determinants. The thymine DNA glycosylase (TDG) was identified as a cell-cycle-regulated co-factor of the tumor suppressor p53. It was also discovered that TDG and p53 control the transcription of p53-dependent genes during self-renewal by binding to ESC-specific cis-regulatory elements. Furthermore, TDG plays a crucial role in promoting molecular heterogeneity during the cell cycle and affects cell fate decisions during the early differentiation of mESCs.
Article
Biochemistry & Molecular Biology
Eishi Aizawa, Evgeniy A. Ozonov, Yumiko K. Kawamura, Charles-Etienne Dumeau, So Nagaoka, Tomoya S. Kitajima, Mitinori Saitou, Antoine H. F. M. Peters, Anton Wutz
Summary: Recent studies have compared the development of mouse germ cells in vitro and in vivo, and identified key factors contributing to the low developmental competence of in vitro-derived oocytes, including misregulation of genes involved in oocyte growth and abnormal epigenetic regulation during early oogenesis.
Review
Endocrinology & Metabolism
Ila Tewari Jasra, Nerea Cuesta-Gomez, Kevin Verhoeff, Braulio A. Marfil-Garza, Nidheesh Dadheech, A. M. James Shapiro
Summary: This review summarizes the roles and mechanisms of mitochondria in somatic cell reprogramming to iPSCs and the metabolic shift associated with directed differentiation into pancreatic beta-like cells.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemical Research Methods
Keishi Kishimoto, Kentaro Iwasawa, Alice Sorel, Carlos Ferran-Heredia, Lu Han, Mitsuru Morimoto, James M. Wells, Takanori Takebe, Aaron M. Zorn
Summary: This study presents a protocol for differentiating hPSCs into organ-specific mesoderm using signaling networks and molecular markers identified from single-cell transcriptomics. The generated mesoderm can be directed towards different organ-specific lineages by manipulating WNT, BMP, RA, and HH pathways. This protocol offers a valuable platform for studying human mesoderm development and can contribute to the engineering of complex foregut tissue for disease modeling and regenerative medicine.
Article
Multidisciplinary Sciences
Takahisa Matsuzaki, Yuko Shimokawa, Hiroyuki Koike, Masaki Kimura, Yuma Kawano, Nao Okuma, Ryuzo Kawamura, Yosuke Yoneyama, Yasuro Furuichi, Fumihiko Hakuno, Shin-Ichiro Takahashi, Seiichiro Nakabayashi, Satoshi Okamoto, Hiromitsu Nakauchi, Hideki Taniguchi, Takanori Takebe, Hiroshi Y. Yoshikawa
Summary: This study developed a mechanically patterned hydrogel for controlling the self-aggregation of cells to generate multicellular organoids. The researchers found that local stiffening and softening had different effects on the self-aggregation process. They also revealed the two-step mechanism of cell self-aggregation and validated it using human pluripotent stem cell-derived hepatic progenitors. These findings are of great importance for future organoid engineering.
Article
Medicine, Research & Experimental
Sumio Hayakawa, Atsushi Tamura, Nikita Nikiforov, Hiroyuki Koike, Fujimi Kudo, Yinglan Cheng, Takuro Miyazaki, Marina Kubekina, Tatiana Kirichenko, Alexander N. Orekhov, Nobuhiko Yui, Ichiro Manabe, Yumiko Oishi
Summary: Recent studies have shown a close relationship between cellular metabolism and the regulation of immune cells. This study reveals that cholesterol metabolism plays an integral role in innate immune responses of macrophages. Cholesterol accumulation in endosomes and lysosomes is crucial for amplification of Myd88 signaling, which is involved in inflammatory macrophage activation. The supramolecular compound PRX is able to inhibit inflammatory macrophage activation by regulating cholesterol trafficking and efflux. These findings highlight the importance of cellular cholesterol metabolism in innate activation of macrophages and suggest its potential as a therapeutic target for inflammatory diseases.
Review
Cell Biology
Rie Ouchi, Hiroyuki Koike
Summary: The discovery of human pluripotent stem cells (PSCs), including embryonic stem cells and induced pluripotent stem cells (iPSCs), has greatly advanced our understanding of human developmental biology and has been used in drug discovery and disease treatments. PSC-derived organoids, which replicate the complex structures of living organs, have been created and used in various fields. iPSC-derived organoids, with their genetic background matching the donor, are useful for disease modeling, pathophysiology elucidation, and drug screening. They are also promising in regenerative medicine as a low-risk alternative to organ transplantation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Tasuku Fujii, Yosuke Yoneyama, Akiko Kinebuchi, Naoki Ozeki, Sho Maeda, Norikazu Saiki, Toyofumi Fengshi Chen-Yoshikawa, Hiroshi Date, Kimitoshi Nishiwaki, Takanori Takebe
Summary: We assessed the enteral ventilation approach in hypoxic porcine models and found that intraanally delivering oxygenated perfluorodecalin can significantly improve arterial oxygenation and reduce carbon dioxide levels. This suggests that the enteral ventilation pathway is an effective means for systemic oxygenation.
Article
Cell & Tissue Engineering
Hasan Al Reza, Zishaan Farooqui, Abid Al Reza, Callen Conroy, Kentaro Iwasawa, Yasuhiro Ogura, Keisuke Okita, Kenji Osafune, Takanori Takebe
Summary: The study uses induced pluripotent stem cells to develop human liver organoids that can mimic conjugation failure phenotype. It elucidates the role of glucocorticoid antagonism in UGT1A1 activation and shows that introducing functional GULO can augment intracellular ascorbate for NRF2 reactivation to protect against hyperbilirubinemia.
Article
Multidisciplinary Sciences
Aiko Watanabe, Hiroyuki Koike, Naoki Kumagami, Shigeki Shimba, Ichiro Manabe, Yumiko Oishi
Summary: After a muscle injury, the process of inflammation, repair, and regeneration is controlled by various myeloid cell types. The role of the transcription factor Arntl in this process was investigated using myeloid cell-specific Arntl conditional knockout mice. The study found that Arntl expression in myeloid cells is required for the recruitment of neutrophils and monocytes, thus playing a crucial role in skeletal muscle regeneration.
SCIENTIFIC REPORTS
(2023)
Article
Biochemical Research Methods
Naritaka Tamaoki, Stefan Siebert, Takuya Maeda, Ngoc-Han Ha, Meghan L. Good, Yin Huang, Suman K. Vodnala, Juan J. Haro-Mora, Naoya Uchida, John F. Tisdale, Colin L. Sweeney, Uimook Choi, Julie Brault, Sherry Koontz, Harry L. Malech, Yasuhiro Yamazaki, Risa Isonaka, David S. Goldstein, Masaki Kimura, Takanori Takebe, Jizhong Zou, David F. Stroncek, Pamela G. Robey, Michael J. Kruhlak, Nicholas P. Restifo, Raul Vizcardo
Summary: Coculturing human induced pluripotent stem cells (hiPSCs) with stromal cells as spheroids can develop into yolk sac-like organoids in a stirred bioreactor, which can generate hematopoietic progenitor cells (HPCs) with lympho-myeloid potential. This method enables bulk production of hiPSC-derived HPCs in a clinical setting, with the advantage of being autologous and xeno-free.
CELL REPORTS METHODS
(2023)
Meeting Abstract
Gastroenterology & Hepatology
Masaki Kimura, Jen-Chieh Chuang, Archana Vijayakumar, Eisuke Murakami, Ryan S. Huss, Robert P. Myers, Mani Subramanian, Takanori Takebe
Meeting Abstract
Gastroenterology & Hepatology
Yasmine Liu, Masaki Kimura, Archana Vijayakumar, Eisuke Murakami, Takanori Takebe, G. Mani Subramanian, Johan Auwerx
Proceedings Paper
Computer Science, Theory & Methods
Yunheng Wu, Masahiro Oda, Yuichiro Hayashi, Takanori Takebe, Shogo Nagata, Cheng Wang, Kensaku Mori
Summary: In this paper, a method is proposed to segment blood vessels in cranial windows from low-contrast and wide-field microscopic images, based on the Attention U-Net framework and clDice. Experimental results show that our method can extract blood vessels better than other methods.
2022 IEEE/CVF CONFERENCE ON COMPUTER VISION AND PATTERN RECOGNITION WORKSHOPS, CVPRW 2022
(2022)
Meeting Abstract
Gastroenterology & Hepatology
Milad Rezvani, Kyle Lewis, Kentaro Iwasawa, Yuqi Cai, Yuka Milton, Masaki Kimura, RanRan Zhang, Praneet Chaturvedi, Takanori Takebe
JOURNAL OF HEPATOLOGY
(2022)
Meeting Abstract
Oncology
Takanori Takebe
Meeting Abstract
Gastroenterology & Hepatology
Kentaro Tominaga, Dan Kechele, Guillermo Sanchez, Heather McCauley, Jacob Enriquez, Simon Vales, Ingrid Jurickova, Lee Denson, Takanori Takebe, Michael Helmrath, Aaron Zorn, James Wells