Article
Biotechnology & Applied Microbiology
Lena Wohlschlager, Florian Csarman, Matea Zrilic, Bernhard Seiboth, Roland Ludwig
Summary: In this study, two efficient processes for the heterologous production of GLOX from Phanerochaete chrysosporium were reported and compared using Pichia pastoris and Trichoderma reesei as expression hosts. The enzymes produced in different hosts showed similar substrate affinities and turnover numbers, with GLOX from both hosts being suitable for further mechanistic characterization and application studies. Additionally, GLOX expressed in Pichia pastoris exhibited slightly higher specific activities due to higher copper loading compared to the enzyme from Trichoderma reesei.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Microbiology
Wenqiang Xu, Yu Fang, Mingyang Ding, Yajing Ren, Xiangfeng Meng, Guanjun Chen, Weixin Zhang, Weifeng Liu
Summary: This study identified a putative sugar transporter encoding gene gat1 in Trichoderma reesei, which showed significant upregulation on xylan and deletion of which resulted in increased xylanase production. Deletion of gat1 also affected the expression of xyn1 and pectinase genes when T. reesei was cultivated with galacturonic acid as the sole carbon source. Transcriptome analysis revealed 255 differentially expressed genes enriched in categories of glycoside hydrolases, lipid metabolism, transporters, and transcriptional factors, suggesting a repressive role of the sugar transporter GAT1 in xyn1 expression and distinct regulatory mechanisms in controlling the expression of different xylanase genes in T. reesei.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Dongmei Lv, Weixin Zhang, Xiangfeng Meng, Weifeng Liu
Summary: Engineering transcription factors can enhance the cost-effective production of lignocellulolytic enzymes for biorefinery processes. A novel mutation (R434L) in xylanase regulator 1 (Xyr1) was found to significantly improve cellulase and xylanase production in Trichoderma reesei. The increased transcription of major cellulase and xylanase genes in the mutant strain (OEXR434L) was verified to be responsible for the improved enzyme production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Carlos Eduardo de Oliveira Nascimento, Lorena Caixeta de Oliveira Simoes, Josiani de Cassia Pereira, Ronivaldo Rodrigues da Silva, Evandro Antonio de Lima, Gabriel Cimonetti de Almeida, Ana Lucia Barretto Penna, Mauricio Boscolo, Eleni Gomes, Roberto da Silva
Summary: This study produced Xylooligosaccharides (XOs) by extracting xylan from sugarcane bagasse and hydrolyzing it using an enzyme. The XOs stimulated the growth of beneficial intestinal bacteria and could be used as a supplement to promote the growth of probiotic strains.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Assel Kiribayeva, Birzhan Mukanov, Dmitriy Silayev, Zhiger Akishev, Yerlan Ramankulov, Bekbolat Khassenov
Summary: Xylanase from Bacillus sonorensis T6 was successfully cloned and expressed in E. coli and P. pastoris. The recombinant xylanases showed optimal activity and stability at specific temperatures and pH ranges. These findings suggest that the xylanase from Bacillus sonorensis T6 has potential applications in various biotechnological processes.
Article
Biotechnology & Applied Microbiology
Vera Novy, Fredrik Nielsen, Daniel Cullen, Grzegorz Sabat, Carl J. Houtman, Christopher G. Hunt
Summary: The study focused on cultivating Trichoderma reesei on different softwood substrates and analyzing the enzyme activity time courses, secretome analysis, and fungal micromorphology in response to changes in substrate characteristics. The results showed that substrate-induced changes in fungal response and the hydrolytic strength of secreted enzymes significantly impacted saccharification efficiencies. Cultivating T. reesei on specific substrates led to an improved hydrolytic strength of the enzyme mixture, potentially reaching the performance of a commercial enzyme preparation.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biochemistry & Molecular Biology
Gautam Anand, Meirav Leibman-Markus, Dorin Elkabetz, Maya Bar
Summary: Plants lack a circulating adaptive immune system, instead relying on a complex and efficient innate immune system based on defense mechanisms triggered by elicitors such as microbe-associated molecular patterns (MAMPs). The fungal MAMP protein Ethylene Inducing Xylanase (EIX) from Trichoderma is a useful tool for studying plant immunity, eliciting defense responses in tobacco and tomato. Xylanases like EIX, hydrolytic enzymes mainly synthesized by fungi, play a role in xylan breakdown and can effectively induce plant defense responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Qinqin Zhao, Zezheng Yang, Ziyang Xiao, Zheng Zhang, Jing Xing, Huiqi Liang, Liwei Gao, Jian Zhao, Yinbo Qu, Guodong Liu
Summary: This study identified alanine 873 as a potential target for engineering XYR1 to improve lignocellulolytic enzyme production in Trichoderma reesei. Mutation of this residue to tyrosine significantly enhanced the production of xylanolytic enzymes, especially when glucose was used as the carbon source.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Su Yan, Yan Xu, Xiao-Wei Yu
Summary: In this study, a xylanase hyper-producing system in T. reesei was established by manipulating transcription factors and overexpressing xylanase, leading to efficient xylan hydrolysis and improved biomass degradation efficiency.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Food Science & Technology
Efsa Panel On Food Contact Materials, Claude Lambre, Jose Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Marcel Mengelers, Alicja Mortensen, Gilles Riviere, Inger-Lise Steffensen, Christina Tlustos, Henk Van Loveren, Laurence Vernis, Holger Zorn, Boet Glandorf, Lieve Herman, Yrjo Roos, Natalia Kovalkovicova, Yi Liu, Simone Lunardi, Giulio Di Piazza, Rita Ferreira de Sousa, Andrew Chesson
Summary: This article introduces a food enzyme produced by a non-genetically modified Trichoderma reesei strain AR-256 and used in seven food manufacturing processes. The estimated dietary exposure of the enzyme indicates that it does not raise safety concerns under the intended conditions of use. However, individuals with salmon allergy may have a risk of allergic reactions.
Article
Biochemistry & Molecular Biology
Protyusha Dey, Amit Roy
Summary: Enzymatic degradation of hemi-cellulosic substrates has garnered industrial attention, requiring a cocktail of enzymes for complete degradation. This study reports the discovery and characterization of a potential endo-1,4-beta-xylanase gene from the mushroom Termitomyces clypeatus, providing a new source for commercial exploitation in the future.
CARBOHYDRATE RESEARCH
(2021)
Article
Fisheries
Zixuan Wu, Yujie Liu, Jiaqi Zheng, Yongzhao Zhou, Kefan Xing, Yuying Sun, Jiquan Zhang
Summary: Arginine kinase is crucial for organisms with high energy demands, and in Exopalaemon carinicauda, it may play a similar role as in other crustaceans. Its expression is predominantly in muscle and can change in response to bacterial challenges. Recombinant expression and purification of EcAK showed similar enzymatic characteristics to AK from Euphausia superba.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ting Miao, Abdul Basit, Junquan Liu, Fengzhen Zheng, Kashif Rahim, Huiqiang Lou, Wei Jiang
Summary: The study improved the production of Myceliophthora thermophila xylanase through signal peptide engineering, resulting in enhanced enzyme activity. Further analysis showed that Mtxyn11C was more effective than commercial xylanase in degrading xylan, and when combined with other xylanolytic enzymes, it improved the hydrolysis efficiency of xylan.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Zhenyang Liu, Sitao Wen, Guogan Wu, Huawei Wu
Summary: A new glycoside hydrolase family 10 (GH10) xylanase gene, ArXyn10c20, from anaerobic rumen microorganism Anaeromyces robustus, was successfully synthesized and expressed. The recombinant xylanase showed optimal pH and temperature of 5.5 and 40 degrees Celsius, respectively. It exhibited stability in a pH range of 5.0-9.0 and its function was enhanced by the addition of 1 mM Cu2+. The hydrolysis products of ArXyn10c20 on beechwood xylan were xylobiose, xylotriose, and xylotetraose. Additionally, when added to bread making, ArXyn10c20 significantly improved dough and bread quality.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yuhao Gao, Na Liu, Yaxin Zhu, Shiyu Yu, Qiulin Liu, Xiangliu Shi, Jianguo Xu, Guoqiang Xu, Xiaomei Zhang, Jinsong Shi, Zhenghong Xu
Summary: This study achieved dual-path synthesis of GSH in yeast by heterologously expressing ScGSH1, ScGSH2, and StGshF. Overexpression of ScADK1 and addition of sodium citrate were found to have a positive impact on GSH synthesis. By optimizing the addition conditions of amino acids, the highest titer and yield of GSH were improved.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Materials Science, Textiles
Yan Luo, Caixia Wang, Lihui Wang, Xuemei Ding, Xiongying Wu
Summary: This study investigates the effect of a novel press washing mode on the appearance and mechanical properties of silk garments compared to hand washing, gentle machine washing, and normal machine washing. The experimental results demonstrate that press washing has the best performance in terms of appearance indicators and elastic recovery. The results provide guidance and ideas for scientific care and optimization.
JOURNAL OF THE TEXTILE INSTITUTE
(2023)
Article
Environmental Sciences
Shuju Fang, Gufeng Li, Hongyu Shi, Junxiu Ye, Hongbin Wang, Xuemei Ding, Lijun Luo, Guizhen Li, Min Yang
Summary: A low-cost functionalization method was used to prepare an efficient adsorbent for ammonia nitrogen from diatomite. The optimized conditions included modification with H2SO4, NaCl, and calcination, resulting in excellent adsorption performance of the functionalized diatomite (DTCA-Na). The adsorption process of ammonia nitrogen followed pseudo-first-order kinetics and Langmuir isothermal model. The removal efficiency of ammonia nitrogen remained above 80% even after multiple adsorption-desorption experiments.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Materials Science, Textiles
Xin Zhao, Caixia Wang, Lihui Wang, Hugh Gong, Xuemei Ding
Summary: Wool fiber is widely used for clothing due to its excellent properties, but it can shrink during laundry. Panasonic has developed a washing machine that uses tapping actions to clean delicate clothes like wool, providing the best appearance and dimensional stability.
TEXTILE RESEARCH JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Guanhua Chen, Yang Zhang, Keying Zhang, Gang Tian, Shiping Bai, Xuemei Ding, Jianping Wang, Li Lv, Quan Tan, Wei Zhao, Qiufeng Zeng
Summary: This study investigates the impact of dietary zinc deficiency on skin properties in broiler chickens and Pekin ducks. The results show that ducks are more sensitive to zinc deficiency in terms of skin weight and composition, while broiler chickens are more sensitive in terms of skin fat content.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Chemistry, Applied
Simeng Qin, Keying Zhang, Xuemei Ding, Shiping Bai, Jianping Wang, Gang Tian, Yue Xuan, Zhuowei Su, Quifeng Zeng
Summary: In this study, the impact of resistant starch (RS) on meat duck intestine and its resident microbiota was investigated. The results showed that dietary RS improved intestinal morphology and barrier function in meat ducks, as evidenced by enhanced expression of tight junction proteins and Mucin-2 gene. RS administration also led to changes in the gut microbiome and metabolome, with increased proportions of Firmicutes and butyrate-producing bacteria, as well as alterations in amino acid and lipid metabolism. These findings provide valuable insights into the mechanism by which RS improves the gut system of ducks.
Article
Engineering, Manufacturing
Mark Gorman, Xuemei Ding, Liam Maguire, Damien Coyle
Summary: The objective of this study is to improve anomaly detection performance and interpretability by using 1-Dimensional Convolutional AutoEncoders (1d-CAE) and Localised Reconstruction Error (LRE). The proposed framework has been validated using the Tennessee Eastman Process (TEP) and LAM 9600 Metal Etcher datasets.
IEEE TRANSACTIONS ON SEMICONDUCTOR MANUFACTURING
(2023)
Article
Green & Sustainable Science & Technology
Yan Luo, Mengfan Yu, Xiongying Wu, Xuemei Ding, Laili Wang
Summary: This study conducted computational case studies on different mask products applicable to low-, medium-, and high-risk scenarios. The results showed that reusable cotton masks and disposable medical masks suitable for low-risk settings have a lower carbon footprint, becoming more environmentally friendly after a certain number of washes. Disposable surgical masks and KN95 respirators have higher carbon footprints, while disposable medical masks and disposable surgical masks are identified as alternatives with better environmental performance in medium- and high-risk environments. The results provide scientific guidance for manufacturers and consumers on the production and use of protective masks.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Agriculture, Dairy & Animal Science
Jianping Wang, Xuemei Ding, Qiufeng Zeng, Shiping Bai, Keying Zhang, Xiangbing Mao, Shengyu Xu, Yong Zhuo, Yue Xuan, Huanwei Peng, Jingbo Liu, Huadong Yin
Summary: This study investigated the effects of 25-hydroxyvitamin D (25OHD) on egg production, egg quality, immune response, and intestinal health of laying hens challenged with Escherichia coli lipopolysaccharide (LPS). The results showed that supplementing 80 mg/kg of 25OHD in diets can improve egg laying rate and feed efficiency, decrease broken egg rate, and enhance intestinal barrier function and antioxidant capacity. Additionally, it can also decrease proinflammatory cytokines levels in laying hens. Therefore, the addition of 25OHD in diets may enhance laying performance and egg quality in LPS-challenged hens.
Article
Materials Science, Textiles
Yuan Yuan, Luo Yan, Shuaitong Liang, Yan Li, Hongjian Qu, Xuemei Ding, Jiping Wang
Summary: This article examines the impact of the consumer use phase of textiles and fashion on carbon and water footprints. Through tracking data from three volunteer families with different demographic characteristics, it is found that consumer behaviors have a significant influence on carbon and water footprints. The comparison between test data and estimated data also reveals the uncertainty between them.
TEXTILE RESEARCH JOURNAL
(2023)
Article
Materials Science, Textiles
Wenjuan Huang, Shuaitong Liang, Hongjuan Zhang, Xuemei Ding, Jiping Wang
Summary: This study analyzed the desorption components of cotton, wool, and polyester fabrics exposed to tobacco smoke and hot pot odor using gas chromatography-mass spectrometry. Different mass diffusion coefficients were proposed for odors with different molecular weights. The results showed that cotton fabrics released more compounds with tobacco smoke and hot pot odor, while wool fabrics had fewer compounds, and 3-ethenylpyridine was not detected. The dynamic model demonstrated the effect of airflow velocity and fabric porosity on nicotine desorption.
AATCC JOURNAL OF RESEARCH
(2023)
Article
Computer Science, Artificial Intelligence
Magda Bucholc, Sofya Titarenko, Xuemei Ding, Callum Canavan, Tianhua Chen
Summary: The study aimed to develop a novel prognostic machine learning framework that utilizes longitudinal information encoded in efficient, cost-effective, and non-invasive markers to identify individuals with mild cognitive impairment (MCI) at risk of developing dementia. The findings showed that incorporating the output of unsupervised learning significantly improved the performance of supervised ML models. This prognostic framework can be applied to improve recruitment in clinical trials and select early interventions for individuals at high risk of developing dementia.
EXPERT SYSTEMS WITH APPLICATIONS
(2023)
Article
Agriculture, Dairy & Animal Science
Hongye Zhang, Mengyu Li, Keying Zhang, Xuemei Ding, Shiping Bai, Qiufeng Zeng, Licui Chu, Danxi Hou, Yue Xuan, Huadong Yin, Jianping Wang
Summary: The objective of this study was to investigate the effects of dietary supplementation with benzoic acid, Enterococcus faecium, and essential oil complex (BEC) on laying hens recovering from coccidia and Clostridium perfringens challenge. Results showed that the addition of BEC improved egg-laying rate, reduced gut damage, and modulated microbial composition. The genera Faecalibacterium and Asterolplasma were positively correlated with egg-laying rate, while the genera Bacteroides and Romboutsia were negatively correlated. The addition of BEC to the diet demonstrated positive effects on the production performance and gut health of laying hens recovering from the challenge.
Article
Agriculture, Dairy & Animal Science
Shanchuan Cao, Dan Guo, Huadong Yin, Xuemei Ding, Shiping Bai, Qiufeng Zeng, Jingbo Liu, Keying Zhang, Xiangbing Mao, Jianping Wang
Summary: This study investigated the effects of fecal microbiota transplantation from highly laying rate donors on the cecal microbiota, intestinal health, and ovarian function in broiler breeders. The transplantation resulted in increased egg-laying rate and hormone levels, as well as decreased proinflammatory cytokine levels in the breeders. It also improved intestinal health, increased hormone receptor gene expression, and affected the apoptosis and cytokine signaling pathway in the ovaries of the breeders.
Article
Materials Science, Textiles
Yuhui Wei, Xue Ling, Gangyu Wang, Zhaowei Su, Xuemei Ding, Zongqian Wang, Kaiming Wu, Wei Pan
Summary: To understand the aging mechanism of down fiber under daily washing and drying conditions, the effects of different treatments on fluffiness, whiteness, microscopic morphology, crystallinity, and molecular structure were observed. Results showed that weak acid or alkali washing conditions and low temperature drying only slightly affected the properties of down fiber. Mechanical agitation had a positive impact on fluffiness and whiteness. However, strong alkali washing and high temperature drying caused significant damage to the fiber. Regardless of the conditions, prolonged mechanical agitation led to performance degradation. Down fiber is best suited for weak acid or alkali washing and low temperature drying with minimal mechanical agitation.
JOURNAL OF NATURAL FIBERS
(2023)
Article
Materials Science, Multidisciplinary
Guanyu Shang, Guoyu Li, Chunsheng Guan, Weisong Zhao, Haoyu Li, Kuang Zhang, Jian Liu, Qun Wu, Xue-Mei Ding, Shah Nawaz Burokur, Xumin Ding
Summary: This paper reports a bifunctional microwave metasurface with an asymmetric bidirectional transmission phase that can be controlled. The metasurface is composed of bilayered double-arrow-shaped structures sandwiching a subwavelength metal grating. The metasurface breaks the symmetry and realizes different functions for opposite propagation directions, such as deflection and focusing, orbital angular momentum (OAM) mode generation, and holographic imaging. The experimental results suggest that the proposed metasurface can be further exploited for various applications.
ADVANCED PHOTONICS RESEARCH
(2023)