Article
Chemistry, Multidisciplinary
Jake W. Wilson, Bo Su, Makoto Yoritate, Jake X. Shi, John F. Hartwig
Summary: We report the iridium-catalyzed, stereoselective conversion of secondary alcohols or ketones to anti-1,3-diols by the silylation of secondary C-H bonds ? to oxygen and oxidation of the resulting oxasilolane. The silylation occurs with high selectivity at a secondary C-H bond ? to oxygen over distal primary or proximal secondary C-H bonds.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Jake W. Wilson, Bo Su, Makoto Yoritate, Jake X. Shi, John F. Hartwig
Summary: In this paper, we report the iridium-catalyzed conversion of secondary alcohols or ketones to anti-1,3-diols. This conversion is achieved through the silylation of secondary C-H bonds and subsequent oxidation of the resulting oxasilolane. The silylation occurs selectively at secondary C-H bonds ? to oxygen, and the catalyst used in this process is formed from a simple bisamidine ligand, which provides a long catalyst lifetime.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Rosa M. Gomila, Antonio Frontera, Edward R. T. Tiekink
Summary: The Cambridge Structural Database was used to identify crystals containing Hg-secondary bonding interactions in mercury(ii) compounds. These interactions form zero, one, and two-dimensional aggregation patterns largely isolated from other directional interactions. The study confirms the existence of spodium bonds with higher directionality and coordination with sulphur donors, providing stabilisation energies greater than conventional hydrogen-bonding interactions.
Article
Biophysics
Anjali Sengar, Marcos Cervantes, Peter M. Kasson
Summary: In this study, a microfluidic assay was developed to differentiate the effects of neutralizing antibodies on attachment and fusion during viral entry. Surprisingly, it was found that some broadly neutralizing antibodies inhibit fusion only, while others inhibit both fusion and viral attachment. This study provides insights into the heterogeneous mechanisms of antibody neutralization within similar recognition sites. Additionally, the developed assay offers a tool for optimizing vaccine design by assessing the antibody response with greater mechanistic resolution.
BIOPHYSICAL JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Antonio Datola, Abhijeet Satwekar, Nadine Barron, Sawako DeRosa, Brittany Tomascak, Jessica Dawson, Angelo Palmese, Mara Rossi
Summary: There is a growing interest in generating Fc-fusion molecules to enhance therapeutic potential. However, manufacturing these molecules poses challenges due to issues like protein folding and structural heterogeneity. This study used mass spectrometry to investigate the reshuffling of disulfide bridges in a specific Fc-fusion molecule, providing insights for consistent and high-quality product manufacturing.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Laszlo Heja, Agnes Simon, Zsolt Szabo, Julianna Kardos
Summary: This study discovered subtype-specific protein stabilization centers (SCs) in Cx GJC, and identified the structure of the GJ interface. The unique trans-GJ SC pattern was found to play a major role in stabilizing the GJC complex. Additionally, clusters of SC patterns close to the GJ interface domain were revealed to orient the interface formation.
Review
Biochemistry & Molecular Biology
Karthik Chamakura, Ry Young
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Microbiology
Shayla Hesse, Manoj Rajaure, Erin Wall, Joy Johnson, Valery Bliskovsky, Susan Gottesman, Sankar Adhya
Article
Biochemistry & Molecular Biology
Jinping Liu, Wei Tang, Anuradha Budhu, Marshonna Forgues, Maria O. Hernandez, Julian Candia, Yujin Kim, Elise D. Bowman, Stefan Ambs, Yongmei Zhao, Bao Tran, Xiaolin Wu, Christopher Koh, Pallavi Surana, T. Jake Liang, Maria Guarnera, Dean Mann, Manoj Rajaure, Tim F. Greten, Zhanwei Wang, Herbert Yu, Xin Wei Wang
Article
Microbiology
Ashley Holt, Jesse Cahill, Jolene Ramsey, Cody Martin, Chandler O'Leary, Russell Moreland, Lori T. Maddox, Thushara Galbadage, Riti Sharan, Preeti Sule, Jeffrey D. Cirillo, Ry Young
Summary: We provide evidence that phiKT produces an antimicrobial peptide for outer membrane disruption during lysis. This protein, designated a disruptin, is a new paradigm for phage lysis and has no similarities to other known lysis genes. The phiKT disruptin may represent the first genetically tractable antimicrobial peptide, facilitating mechanistic analyses.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Engineering, Environmental
Harvey N. Summerlin, Cicero C. Pola, Karthikeyan R. Chamakura, Ry Young, Terry Gentry, Eric S. McLamore, Raghupathy Karthikeyan, Carmen L. Gomes
Summary: This study investigated the cultivation of romaine lettuce with inoculated wastewater effluent, tracking the prevalence of AP205 bacteriophage. Results showed a direct relationship between AP205 concentrations and contamination levels in foliage, leachate, and soil, with an increase in bacteriophage accumulation throughout cultivation.
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING
(2021)
Article
Microbiology
Denise Mehner-Breitfeld, Jan Michel Frederik Schwarzkopf, Ry Young, Kiran Kondabagil, Thomas Brueser
Summary: The periplasmic antiholin RI is now found to have a cleavable signal peptide in its N-terminal trans-membrane domain, contrary to previous beliefs. This discovery suggests a fundamentally different interpretation of phage lysis regulation and the structure of the soluble domains of the RI-T complex.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Yi Duan, Ry Young, Bernd Schnabl
Summary: The Perspective explores the effects of bacteriophages on the gut microbiota and the potential applications of phage therapy for gastrointestinal diseases, while also discussing limitations and challenges. The emergence of multidrug-resistant bacteria and a better understanding of the role of the gut microbiota in human health and disease have brought bacteriophages back into focus.
NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY
(2022)
Article
Microbiology
Jake S. Chamblee, Jolene Ramsey, Yi Chen, Lori T. Maddox, Curtis Ross, Kam H. To, Jesse L. Cahill, Ry Young
Summary: Host cell lysis is the final step of bacteriophage infection cycle. Mu phage lacks a holin protein for cell lysis and instead uses a releasin protein gp25 for the release of endolysins. This study provides insights into the mechanism of lysis in Mu phage and its evolutionary implications.
Article
Microbiology
Janki Patel, Brenda Godoy, James Clark, Ben Burrowes, Ry Young, Mei Liu
Summary: In this study, we report the complete genome of the S. maltophilia myophage Marzo, which is closely related to S. maltophilia phages IME-SM1 and Mendera.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Microbiology
Nancy Wang, James Garcia, Jay Clark, Tram Le, Ben Burrowes, Ry Young, Mei Liu
Summary: A phage called S. maltophilia siphophage Silvanus was isolated, and its genome, consisting of 45,678 base pairs, does not show close similarity to known phages. It is predicted to use cos-type packaging based on the similarity of its large terminase subunit to that of phage HK97. This opportunistic Gram-negative bacterium, Stenotrophomonas maltophilia, has the capability to cause respiratory infections.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Microbiology
Mike Feiss, Sankar Adhya, Costa Georgopoulos, Roger W. Hendrix, Graham F. Hatfull, Eddie B. Gilcrease, Sherwood R. Casjens, Jolene Ramsey, Ry Young
Summary: Recombinational hybrids between phage lambda and its relatives played a crucial role in the early days of molecular biology. In this study, we present the complete genome sequences of lambdoid phages 21 and 434, as well as three lambda hybrids. Additionally, we describe 434B, where the entire lysis gene region was replaced by cryptic prophage sequences.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Microbiology
Adam Tomaszewski, Daniel Mora, James Clark, Tram Le, Ry Young, Mei Liu
Summary: This study presents a bacteriophage called Sigurd, which is associated with antibiotic-resistant infections. The genome of Sigurd is 41,811-bp in length and has a unique structure.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Biochemistry & Molecular Biology
Benjamin A. Adler, Karthik Chamakura, Heloise Carion, Jonathan Krog, Adam M. Deutschbauer, Ry Young, Vivek K. Mutalik, Adam P. Arkin
Summary: This study used a high-throughput genetic screen to identify genome-wide host suppressors of diverse single-gene lysis factors (Sgls). In addition to validating known mechanisms, the researchers discovered that the Sgl of PP7, a Pseudomonas aeruginosa ssRNA phage, targets MurJ, the flippase responsible for lipid II export, which was previously shown to be the target of the Sgl of coliphage M. These unrelated Sgls, which are predicted to have opposite membrane topology, represent a case of convergent evolution. Furthermore, the genetic screens revealed a common set of multicopy suppressors for uncharacterized Sgls, suggesting a shared or similar mechanism of action.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Nkechi Enwuru, Jason J. Gill, Katri P. Anttonen, Christian A. Enwuru, Ry Young, Akinloye O. Coker, Jeffrey D. Cirillo
Summary: The study evaluated the antibacterial and biofilm removal activities of lytic phage against multi-drug-resistant P. aeruginosa. The phage demonstrated wide stability range and species specificity, showing strong bacteriolytic activity against biofilm-forming multi-drug-resistant strains.
BENI-SUEF UNIVERSITY JOURNAL OF BASIC AND APPLIED SCIENCES
(2021)
Article
Microbiology
Gunaraj Dhungana, Rajani Malla, Manoj Rajaure, Sankar Adhya
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)