Article
Chemistry, Analytical
Robert S. Plumb, Thomas McDonald, Paul D. Rainville, Jason Hill, Lee A. Gethings, Kelly A. Johnson, Ian D. Wilson
Summary: Vacuum jacketed column (VJC) technology can significantly improve the performance of UHPLC systems, enhancing chromatographic separation and increasing detection capabilities for metabolites. Using the VJC system can reduce peak tailing, increase peak capacity, and enhance the intensity and response of MS peaks.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Thomas Andrieu, Therina du Toit, Bruno Vogt, Michael D. Mueller, Michael Groessl
Summary: We developed a liquid chromatography high-resolution mass spectrometry method for the absolute quantification of 51 steroids in human serum and peritoneal fluid, allowing accurate and precise analysis of classical and non-classical steroids. The approach enables post hoc analysis of clinical samples for any steroid once a standard becomes available. It has been demonstrated to evaluate differences in steroid content between peripheral serum and peritoneal fluid across menstrual cycle phases and the effect of the synthetic gestagen dienogest on the steroid metabolome.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Endocrinology & Metabolism
Tiina Jaeaeskelaeinen, Olli Kaerkkaeinen, Jenna Jokkala, Anton Klavus, Seppo Heinonen, Seppo Auriola, Marko Lehtonen, Kati Hanhineva, Hannele Laivuori
Summary: Maternal metabolism undergoes significant changes during pregnancy, yet few studies have explored these changes using metabolomics technologies. We utilized LC-MS based non-targeted metabolomics to compare the metabolic profiles of serum samples from pre-eclamptic and healthy women in early and late pregnancy. Our study found that the progression of pregnancy had a major impact on serum metabolite profiles, with distinct changes observed between early and late pregnancy samples. The metabolic signatures of pregnancy were largely similar between pre-eclamptic and healthy women, with only a few specific metabolites showing differences.
Article
Chemistry, Applied
Lei Ye, Bo Zhang, Jie Zhou, Xiaoping Zhang, Wei Tan, Xiaolin Li
Summary: This study investigates the metabolic profiles of Auricularia cornea grown on pinecone substrates, revealing a rich variety of amino acid metabolites and significant differences related to protein synthesis. The findings contribute valuable insights into the nutritional and medicinal properties of A. cornea grown on a pinecone substrate.
Article
Chemistry, Applied
Alexandr Mikyska, Martin Dusek, Vladimira Jandovska, Jana Olsovska, Tomas Vrzal
Summary: A fast and simple method for qualitative profiling of proanthocyanidin (PAC) composition in hops was developed. The PAC varietal profiles were found to be closely linked with the genetic origin of the hops and can be important for variety selection.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Oncology
Xiangping Lin, Lucie Lecuyer, Xinyu Liu, Mohamed N. Triba, Melanie Deschasaux-Tanguy, Aicha Demidem, Zhicheng Liu, Tony Palama, Adrien Rossary, Marie-Paule Vasson, Serge Hercberg, Pilar Galan, Philippe Savarin, Guowang Xu, Mathilde Touvier
Summary: Correct identification of subjects at high risk is crucial for preventing and screening prostate cancer. Metabolite analysis in biofluids has shown potential in identifying novel biomarkers for PCa. The study found dysregulation of amino acids and sphingolipid metabolism to be associated with future risk of PCa, highlighting 10 specific metabolites.
Article
Chemistry, Analytical
Sylvia K. Neef, Ute Hofmann, Thomas E. Muerdter, Matthias Schwab, Mathias Haag
Summary: The suitability of a capillary hydrophilic interaction chromatography (CapHILIC) -QTOF-MS system for non-targeted metabolomics was evaluated and compared with a narrow-bore hydrophilic interaction chromatography (HILIC) -QTOF-MS system. Both platforms detected a similar number of features and performed well in terms of retention time stability, analytical repeatability, and signal intensity. The use of CapHILIC increased the signal areas of all metabolites, though the signal-to-noise ratio was only improved for 50% of the metabolites. Further optimization of CapHILIC conditions improved reproducibility and signal intensity.
Article
Plant Sciences
Tian Wu, Rui Zou, Dian Pu, Zengquan Lan, Bingyu Zhao
Summary: Intercropping of Chinese chestnut and tea plants can significantly enhance the quality of tea leaves, as shown by metabolomics analysis. The altered metabolites, especially in amino acid metabolism, play a crucial role in improving the taste and health benefits of tea leaves in the intercropping system. This study highlights the potential for improving tea quality through metabolic pathways by adopting intercropping systems.
Article
Veterinary Sciences
Jiayuan Mo, Le Sun, Juanru Cheng, Yujie Lu, Yaochang Wei, Guangsheng Qin, Jing Liang, Ganqiu Lan
Summary: Follicular atresia is a major factor limiting the reproductive power of domestic animals, with unclear molecular mechanisms at the metabolic level. This study found that porcine atretic follicles have lower levels of lipids such as phospholipids and higher levels of amino acids and bile acids compared to healthy follicles. Disorders in amino acid and cholic acid metabolism may contribute to porcine follicular atresia.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biochemical Research Methods
Fayaj A. Mulani, Sharvani S. Nandikol, Jagadeesh S. Kajjihundi, Niranjana Pathappa, Sharanappa Puttappa, Hirekodathakallu Thulasiram
Summary: Meliaceae plants are widely used in agriculture, folklore, and traditional medicine, and contain diverse limonoids with various bioactivities. Accurate detection of these compounds from plant tissue extracts or biological samples is challenging. In this study, a new method was developed for the identification, quantification, and characterization of limonoids in different plant tissue extracts using minimal plant material.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Michele Protti, Marco Cirrincione, Sarah Palano, Eleonora Poeta, Giorgia Babini, Maria Chiara Magnifico, Simona Nicole Barile, Nicola Balboni, Francesca Massenzio, Mohammadreza Mahdavijalal, Federico M. Giorgi, Roberto Mandrioli, Francesco M. Lasorsa, Barbara Monti, Laura Mercolini
Summary: Accurate characterization of metabolic profiles is essential for studying cellular metabolic pathways and understanding human diseases. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach was developed for the qualitative and quantitative analysis of compounds in cell cultures. The method showed good sensitivity, selectivity, linearity, and precision, making it suitable for studying biochemical pathways in central nervous system diseases.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Chanyi Li, Jiaying Qin, Wuping Liu, Bo Lv, Ning Yi, Jinfeng Xue, Zhigang Xue
Summary: The study analyzed the relationship between the main metabolites involved in the Hcy metabolic pathway and diabetes mellitus (DM). The content of Hcy, cysteine, taurine, pyridoxamine, methionine, and choline were significantly increased in the DM group. Hcy, cysteine, taurine, methionine, and choline can be used as risk factors for diabetes diagnosis and severity assessment.
Article
Food Science & Technology
Guirong Zhang, Li Li, Jun Liu, Ji Cai, Junjie Fu, Na Li, Hong Cao, Hongwei Xu, Ying Zhang, Rong Cao
Summary: This study identified the characteristic components of Sichuan Sun vinegar and discovered various physiologically active substances. These findings provide information for the purification and strengthening of beneficial substances in vinegar.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Tong-Meng Yan, Yu Pan, Meng-Lan Yu, Kua Hu, Kai-Yue Cao, Zhi-Hong Jiang
Summary: A new method combining site-specific RNase H digestion with LC-MS/MS and RNA-seq techniques has been developed for comprehensive profiling of tRNA modifications at single-base resolution, improving information content of collision-induced dissociation spectra and reducing false positives.
ANALYTICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Xiaohan Jia, Hongfan Chen, Xinyi Wang, Xin Nie, Lu Xiang, Dayu Liu, Zhiping Zhao
Summary: This study investigated the effects of ultra-long-term fermentation on the formation of non-volatile metabolites in Chinese solid-fermented kohlrabies. LC-MS/MS-based non-targeted metabolomics coupled with multivariate statistical analysis were used to analyze kohlrabies fermented for 5, 8, and 11 years. The results identified differential metabolites and pathways associated with the fermentation process, providing insight into the formation of non-volatile metabolites and their potential applications in cooking Chinese dishes.
FERMENTATION-BASEL
(2023)
Article
Genetics & Heredity
Yanping Lv, Yuqi Yi, Rongchun Han, Xiaohui Tong, Hiroki Takahashi
Summary: The chloroplast genome of Abutilon theophrasti is 160,331 bp in length, encoding 76 genes, and shows a close relationship with plants from the genus Gossypium.
MITOCHONDRIAL DNA PART B-RESOURCES
(2021)
Article
Microbiology
Takahito Toyotome, Kenji Onishi, Mio Sato, Yoko Kusuya, Daisuke Hagiwara, Akira Watanabe, Hiroki Takahashi
Summary: Azole resistance in Aspergillus fumigatus is mainly achieved through mutations in the Cyp51A gene and tandem repeat acquisition in the promoter region. However, this study identified other gene mutations such as MfsD, AgcA, UbcD, AbcJ, and RttA contributing to decreased susceptibility to azoles. These findings suggest that various genetic mutations play a role in reducing susceptibility to medical and agricultural azole fungicides.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Hiroki Takahashi, Sayoko Oiki, Yoko Kusuya, Syun-ichi Urayama, Daisuke Hagiwara
Summary: Researchers focused on eight strains of azole-resistant A. fumigatus isolated from a single tulip bulb for sale in Japan, revealing a possible link between increased drug resistance and the agricultural use of azoles. The study also showed historical genetic recombination among the strains, with some strains displaying tolerance to other classes of fungicides and related amino acid alterations in target proteins.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Dermatology
Hidetaka Majima, Teppei Arai, Yoko Kusuya, Hiroki Takahashi, Akira Watanabe, Yasunari Miyazaki, Katsuhiko Kamei
Summary: This study demonstrates genetic differences in cyp51A polymorphisms of A. fumigatus between Japan and Europe. The most prevalent polymorphism in Japanese strains is the cyp51A(N248K) mutation.
Article
Cell Biology
Ayako Tonoki, Saki Nagai, Zhihua Yu, Tong Yue, Sizhe Lyu, Xue Hou, Kotomi Onuki, Kaho Yabana, Hiroki Takahashi, Motoyuki Itoh
Summary: This study identified the gyc beta gene as a negative regulator of age-related memory impairment (AMI) in fruit flies. It was shown that inhibition of the NO-sGC pathway enhanced intermediate-term memory (ITM) in aged individuals, suggesting a potential mechanism underlying memory impairment in aging.
Article
Genetics & Heredity
Cai Bian, Yoko Kusuya, Daisuke Hagiwara, Sayaka Ban, Yu Lu, Masaki Nagayama, Hiroki Takahashi
Summary: This study reports the function of a Ras-GAP protein AfgapA in A. fumigatus for the first time, revealing its crucial role in fungal adaptation to hypoxic environments and its impact on phenotypic expression.
Article
Genetics & Heredity
Yoko Kusuya, Cai Bian, Daisuke Hagiwara, Sayaka Ban, Hiroki Takahashi
Summary: The transcription factor clcA in Aspergillus fumigatus plays an important role in hyphal growth, conidiogenesis, and copper adaptation.
Article
Microbiology
Masaharu Tsuji, Jun-Ichi Ishihara, Yasuhiro Gotoh, Tetsuya Hayashi, Hiroki Takahashi
Summary: This article reports a new yeast species, Cystobasidium ongulense, found in East Antarctica, with cold active lipases and cellulases that are active even at subzero temperatures.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Microbiology
Masaharu Tsuji, Jun-ichi Ishihara, Atsushi Toyoda, Hiroki Takahashi
Summary: Cystobasidium tubakii, a new basidiomycetous yeast species, was discovered in East Ongul Island, East Antarctica. This species can grow at subzero temperatures and does not require amino acids and vitamins for growth.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Biology
Masatoshi Miyakoshi, Teppei Morita, Asaki Kobayashi, Anna Berger, Hiroki Takahashi, Yasuhiro Gotoh, Tetsuya Hayashi, Kan Tanaka
Summary: Glutamine synthetase (GS) is a crucial enzyme for nitrogen assimilation under nitrogen limiting conditions. This study reveals that an sRNA GlnZ and a transcriptional regulator Nac both play roles in repressing the expression of the sucA component of 2-oxoglutarate dehydrogenase, thereby controlling the metabolic flow in response to nitrogen availability.
Article
Microbiology
Masaharu Tsuji, Jun-ichi Ishihara, Atsushi Toyoda, Hiroki Takahashi, Sakae Kudoh
Summary: We report on the high-quality genome sequence of the Mrakia gelida MGH-2 strain, analyzed using PacBio Sequel and HiSeq 2500 instruments.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
Isato Yoshioka, Hiroki Takahashi, Yoko Kusuya, Takashi Yaguchi, Akira Shibata, Kohtaro Kirimura
Summary: Aspergillus lacticoffeatus WU-2020 is a citric acid hyperproducer that is suitable for solid culture. We present a high-quality draft of its genome sequence (35.9 Mb), consisting of 11 scaffolds and containing 11,490 genes. Additionally, we report the 31.3 kb mitochondrial genome.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Chemistry, Analytical
Seito Shijo, Daiki Tanaka, Tetsushi Sekiguchi, Jun-ichi Ishihara, Hiroki Takahashi, Masashi Kobayashi, Shuichi Shoji
Summary: We developed a microfluidic device that utilizes dielectrophoresis to selectively trap and extract droplets from multiple trapping pockets. The device includes a main microchannel, five droplet-trapping pockets with side channels, and drive electrode pairs positioned around the trapping pockets. Agarose droplets containing biological samples were successfully trapped in the pockets due to flow resistance differences. Under an applied voltage of 500 V, target droplets were selectively extracted using dielectrophoretic force. The device allows for the screening of encapsulated single cells and the targeted extraction of desired droplets.
Article
Biology
Yikelamu Alimu, Yoko Kusuya, Takako Yamamoto, Kana Arita, Naofumi Shigemune, Hiroki Takahashi, Takashi Yaguchi
Summary: This study explores the resistance mechanism of Purpureocillium lilacinum to polyhexamethylene biguanide hydrochloride (PHMB). Genetic mutations were found in PHMB-resistant strains of P. lilacinum, and the knockout of the mutant gene resulted in reduced resistance to PHMB. The adenosine deaminase gene (ADA) was identified as a potential important resistance factor in PHMB-resistant P. lilacinum.
BIOCONTROL SCIENCE
(2022)
Article
Biology
Takako Yamamoto, Yikelamu Alimu, Hiroki Takahashi, Yoko Kusuya, Kouichi Hosoya, Naofumi Shigemune, Satoshi Nagai, Takashi Yaguchi
Summary: The study revealed that Purpureocillium lilacinum is significantly more resistant to PHMB than previously reported, with an unidentified mechanism of drug resistance.
BIOCONTROL SCIENCE
(2021)
