Journal
BMC BIOCHEMISTRY
Volume 13, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1471-2091-13-25
Keywords
Dinitrosopiperazine; Carcinogen; Nasopharyngeal carcinoma; Metastasis; Quantitative proteomics
Categories
Funding
- National Natural Science Foundation of China [81071718, 81000881, 30973400]
- Foundation of State Key Laboratory of Oncology in South China [HN2011-04]
- Fundamental Research Funds for the Central Universities [21611612]
Ask authors/readers for more resources
Background: Nasopharyngeal carcinoma (NPC) has a high metastatic feature. N,N'-Dinitrosopiperazine (DNP) is involved in NPC metastasis, but its mechanism is not clear. The aim of this study is to reveal the pathogenesis of DNP-involved metastasis. 6-10B cells with low metastasis are from NPC cell line SUNE-1, were used to investigate the mechanism of DNP-mediated NPC metastasis. Results: 6-10B cells were grown in DMEM containing H-2(4)-L-lysine and C-13(6) N-15(4)-L-arginine or conventional L-lysine and L-arginine, and identified the incorporation of amino acid by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Labeled 6-10B cells were treated with DNP at 0-18 mu M to establish the non-cytotoxic concentration (NCC) range. NCC was 0-10 mu M. Following treatment with DNP at this range, the motility and invasion of cells were detected in vitro, and DNP-mediated metastasis was confirmed in the nude mice. DNP increased 6-10B cell metastasis in vitro and vivo. DNP-induced protein expression was investigated using a quantitative proteomic. The SILAC-based approach quantified 2698 proteins, 371 of which showed significant change after DNP treatment (172 up-regulated and 199 down-regulated proteins). DNP induced the change in abundance of mitochondrial proteins, mediated the status of oxidative stress and the imbalance of redox state, increased cytoskeletal protein, cathepsin, anterior gradient-2, and clusterin expression. DNP also increased the expression of secretory AKR1B10, cathepsin B and clusterin 6-10B cells. Gene Ontology and Ingenuity Pathway analysis showed that DNP may regulate protein synthesis, cellular movement, lipid metabolism, molecular transport, cellular growth and proliferation signaling pathways. Conclusion: DNP may regulate cytoskeletal protein, cathepsin, anterior gradient-2, and clusterin expression, increase NPC cells motility and invasion, is involved NPC metastasis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available