Journal
BMB REPORTS
Volume 45, Issue 11, Pages 665-670Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
DOI: 10.5483/BMBRep.2012.45.11.261
Keywords
Bombyx mori; Chitin-binding proteins; LTQ-Orbitrap; Peritrophic matrix; Proteome
Categories
Funding
- National Basic Research Program of China [2012CB114604]
- National Hi-Tech Research and Development Program of China [2011AA100306]
- National Natural Science Foundation [30972147]
- Doctorial Innovation Fund of Southwest University [Kb2009003]
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The insect midgut epithelium is generally lined with a unique chitin and protein structure, the peritrophic membrane (PM), which facilitates food digestion and protects the gut epithelium. We used gel electrophoresis and mass spectrometry to identify the extracted proteins from the silkworm PM to obtain an in-depth understanding of the biological function of the silkworm PM components. A total of 305 proteins, with molecular weights ranging from 8.02 kDa to 788.52 kDa and the isoelectric point; ranging from 3.39 to 12.91, were successfully identified. We also found several major classes of PM proteins, i.e. PM chitin-binding protein, invertebrate intestinal mucin, and chitin deacetylase. The protein profile provides a basis for further study of the physiological events in the PM of Bombyx mori. [BMB Reports 2012; 45(11): 665-670]
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