Article
Engineering, Biomedical
Juhi Chakraborty, Julia Fernandez-Perez, Kenny A. van Kampen, Subhadeep Roy, Tim ten Brink, Carlos Mota, Sourabh Ghosh, Lorenzo Moroni
Summary: Engineering biomimetic cellular microenvironments has been a priority in regenerative medicine. This study presents a 3D bioprinting approach using GelMA and SF-G bioinks to fabricate arch-like constructs for cartilage regeneration. The SF-G constructs showed higher cell proliferation and superior collagen network and chondrogenesis compared to GelMA constructs. Protein-protein interaction and gene analysis validated the in vitro results, emphasizing the role of SF-G in regulating signaling pathways. The 3D bioprinted arch-like constructs have great potential for cartilage regeneration.
Article
Biochemistry & Molecular Biology
Shuai Wei, Yu Wang, Yu Sun, Leilei Gong, Xiu Dai, Haoye Meng, Wenjing Xu, Jianxiong Ma, Qian Hu, Xinlong Ma, Jiang Peng, Xiaosong Gu
Summary: Compared with most nondegradable or slowly degradable bone repair materials, bioactive biodegradable porous scaffolds with certain mechanical strengths can promote the regeneration of both new bone and vasculature while the cavity created by their degradation can be replaced by the infiltration of new bone tissue. A three-dimensional porous biomimetic composite scaffold with a two-component SF-MC system was constructed based on the advantages of mineralized collagen (MC) and silk fibroin (SF). The SF-MC scaffold showed good osteogenic induction and cell proliferation abilities, and in vivo experiments confirmed its efficacy in stimulating vascular and bone regeneration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Giriprasath Ramanathan, Grace Felciya Sekar Jeyakumar, Uma Tiruchirapalli Sivagnanam, Pedro Fardim
Summary: In this study, a porous hybrid matrix (CEL-COL-SF) was fabricated using microcrystalline cellulose (CEL), collagen (COL), and silk fibroin (SF). The hybrid matrix exhibited interconnected porous architecture, excellent oxygen permeability, and promoted cell adhesion, proliferation, migration, and minerals deposition. It also showed excellent biocompatibility and cell proliferation when tested with fibroblast and osteoblast-like cells. The matrix demonstrated great potential in bone tissue engineering, as it exhibited the sequential events related to bone regeneration and mimicked the atomic ratio of calcium to phosphate in bone.
PROCESS BIOCHEMISTRY
(2023)
Review
Polymer Science
Jiankang Chen, Jie Liu, Wen Yang, Ying Pei
Summary: A catalyst determines the mechanism of organic chemical reactions and biopolymers provide new opportunities for catalyst construction due to their biocompatibility, environmental friendliness, sustainability, and low cost. The use of collagen and silk fibroin materials for catalyst construction will revolutionize electronic devices and systems. This review summarizes the structure, origin, and processing methods of collagen and silk fibroin, as well as their advantages and applicability in catalyst construction. It also discusses the state-of-the-art design, fabrication, and application of collagen- and silk-based catalysts as carriers and precursors, and evaluates the challenges and prospects in their development.
Article
Engineering, Biomedical
Prisana Pripatnanont, Chanokpim Chankum, Jirut Meesane, Jutakan Thonglam
Summary: The study aimed to fabricate semi-resorbable membranes using silk fibroin with glycerol plasticizer and fish collagen, and compared them to a commercial collagen membrane. The semi-resorbable membrane based on silk fibroin-glycerol exhibited good physical and mechanical properties, along with promoting osteoblastic cell proliferation and differentiation.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Zong-Ju Chen, Hui-Hong Shi, Liang Zheng, Hua Zhang, Yu-Ying Cha, Hui-Xian Ruan, Yi Zhang, Xiu-Cheng Zhang
Summary: The composite aerogel with a dual network structure composed of sheet-like silk fibroin and reticular cellulose wrapping nano-hydroxyapatite on the surface showed high mechanical strength and toughness. In vitro cell culture demonstrated that HEK-293T cells had a high ability of adhesion, proliferation and differentiation on the composite aerogels, indicating potential application in bone tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Rucha Deshpande, Swati Shukla, Raeesa Sayyad, Shalmali Salunke, Anuya Nisal, Premnath Venugopalan
Summary: This study compared the safety and efficacy of silk fibroin-based bone void fillers with ceramic fillers and evaluated their impact on stem cell differentiation. Results demonstrated that SF scaffolds promoted a better differentiation of stem cells into osteoblast lineage compared to ceramic scaffolds.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Minghui Li, Jiaqian You, Qiuyue Qin, Manxuan Liu, Yixin Yang, Kewen Jia, Yidi Zhang, Yanmin Zhou
Summary: Bone tissue engineering (BTE) utilizes silk fibroin (SF) to regulate bone regeneration and stimulate bone formation. SF is a promising material due to its tunable mechanical properties, biodegradability, and biocompatibility. This review introduces the structure and characteristics of SF, summarizes its immunological mechanism for osteogenesis, and emphasizes the various forms of SF biomaterials and their development prospects in BTE. Biomaterials based on SF have great potential in bone tissue engineering, and this review guides future design and research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Physical
Federica Paladini, Mauro Pollini
Summary: Bone tissue engineering is a multidisciplinary research field that aims to create biological substitutes for bone regeneration. The selection of appropriate biomaterials and structures remains a challenge, and silk fibroin is considered a valuable option due to its unique biological properties and processability.
Article
Engineering, Biomedical
Lya Piaia, Simone S. Silva, Joana M. Gomes, Albina R. Franco, Emanuel M. Fernandes, Flavia C. M. Lobo, Luisa C. Rodrigues, Isabel B. Leonor, Marcio C. Fredel, Gean Salmoria, Dachamir Hotza, Rui L. Reis
Summary: Tissue engineering provides new approaches to manage bone regeneration by introducing scaffolds that can be autografts and/or allografts. The chitosan/beta-tricalcium phosphate-based scaffolds developed in this study have excellent biological and mechanical performance, making them suitable for bone tissue engineering.
BIOMEDICAL MATERIALS
(2022)
Review
Biotechnology & Applied Microbiology
Hao Wu, Kaili Lin, Cancan Zhao, Xudong Wang
Summary: Silk fibroin (SF) scaffolds offer excellent biocompatibility and mechanical properties, making them promising candidates for bone regeneration. Further research on fabrication techniques and modifications to enhance osteogenesis show great potential for improving clinical translation and outcomes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Sowmya Selvaraj, Chandrasekar Inbasekar, Suryalakshmi Pandurangan, Nishad Fathima Nishter
Summary: Silk fibroin nanofibers with biocompatible collagen coating and bioactive small molecules such as sinomenine hydrochloride and kaempferol hydrate show potential in skin tissue engineering for wound healing. The collagen-coated silk nanofibers exhibit improved biocompatibility and fibroblast viability compared to uncoated scaffolds. The incorporation of kaempferol hydrate enhances antioxidant properties and promotes wound healing by reducing inflammation and enhancing collagen deposition.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2023)
Article
Engineering, Biomedical
Zhinan Mao, Xuewei Bi, Chengai Wu, Yufeng Zheng, Xiong Shu, Sujun Wu, Juan Guan, Robert O. Ritchie
Summary: In this study, a cartilage-biomimetic silk fibroin-based scaffold with controlled sequential release of bioactive molecules was developed for cartilage repair. The scaffold exhibited excellent structural stability and mechanical properties, providing a desirable 3D microenvironment for cartilage reconstruction. In vitro and in vivo studies showed that the scaffold promoted the recruitment and differentiation of stem cells, leading to in situ cartilage regeneration. This biomimetic scaffold shows promising potential for improved cartilage tissue engineering.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Cell Biology
Zhi-Hu Zhao, Xin-Long Ma, Bin Zhao, Peng Tian, Jian-Xiong Ma, Jia-Yu Kang, Yang Zhang, Yue Guo, Lei Sun
Summary: This study developed a naringin-inlaid composite scaffold for repairing bone defects with superior osteogenic and angiogenic activities compared to other scaffolds. Through gene microarray and in vitro experiments, it was found that naringin facilitated mesenchymal stem cell growth and osteogenic differentiation, enhancing the potential of the NG/SF/HAp scaffold for bone regeneration.
CELL PROLIFERATION
(2021)
Article
Engineering, Biomedical
Yuet Cheng, Gu Cheng, Congyong Xie, Chengcheng Yin, Xiangyang Dong, Zhi Li, Xue Zhou, Qun Wang, Hongbing Deng, Zubing Li
Summary: A silk fibroin hydrogel reinforced by short silica nanoparticles-distributed-silk fibroin nanofibers with superior osteoinductive properties was fabricated for treating bone defects. The composite scaffold mimics the extracellular matrix of native bone and enhances mechanical properties. In vivo studies confirmed successful bone repair using this composite hydrogel.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Joo-Hyung Yoon, Dae-Won Kim, Suk Keun Lee, Seong-Gon Kim
Summary: This study revealed that systemic administration of 4HR increased the weight of the submandibular glands in male rats. Furthermore, the expression levels of testosterone and VEGF in the submandibular glands were increased due to 4HR administration.
HEAD & FACE MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Yei-Jin Kang, Dae-Won Kim, Xiangguo Che, Je-Yong Choi, Seong-Gon Kim
Summary: This study demonstrates that 4HR has the potential to restore the loss of function in mutant p53 cancer cells through acetylation and phosphorylation of p53 and inhibition of HDAC4.
APPLIED SCIENCES-BASEL
(2022)
Review
Clinical Neurology
Parsa Firoozi, Saulo Gabriel Moreira Falci, Seong-Gon Kim, Leon A. A. Assael
Summary: The study aimed to evaluate the evidence from meta-analyses on complementary and nonpharmacological treatment options for post-surgical pain management after third molar surgery. Results showed that platelet-rich fibrin and ozone therapy were more effective in reducing pain compared to other complementary interventions.
PAIN RESEARCH & MANAGEMENT
(2022)
Editorial Material
Chemistry, Multidisciplinary
Seong-Gon Kim
APPLIED SCIENCES-BASEL
(2023)
Review
Medicine, Research & Experimental
Yei-Jin Kang, Sang-Shin Lee, Seong-Gon Kim
Summary: A 75-year-old male patient presented with a 35-mm fluctuant lesion on the floor of the mouth. The lesion was a dome-shaped non-infiltrating angiolipoma, and it was successfully excised using an intraoral approach. This is the first reported case of this type of tumor being treated in this way in an elderly patient.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Seong-Gon Kim, Young-Wook Park, Ji-Hyeon Oh
Summary: This retrospective study analyzed the changes in condylar position following mandibular setback surgery with manual guidance. The results showed significant anterior-lateral-inferior movement of the condyle within 1 week after surgery, but the condyle returned to its original position over time.
APPLIED SCIENCES-BASEL
(2023)
Review
Chemistry, Multidisciplinary
Jwa-Young Kim, Yong-Chan Lee, Seong-Gon Kim, Umberto Garagiola
Summary: This comprehensive review explores the integration of 3D printing and Virtual Surgical Planning (VSP) in orthognathic and oral maxillofacial surgery, which enables personalized surgical methods, precise planning through tactile 3D models, and accurately designed surgical guides for improved patient-centric care and predictable outcomes.
APPLIED SCIENCES-BASEL
(2023)
Review
Biochemistry & Molecular Biology
Jwa-Young Kim, Seong-Gon Kim, Umberto Garagiola
Summary: The potential of sericin, a protein derived from silkworms, is explored for bone graft applications due to its biocompatibility, hydrophilic nature, and cost-effectiveness. It has antioxidant, anti-inflammatory, and UV-resistant properties that contribute to a healthier bone-healing environment, and its incorporation into 3D-printed grafts could lead to personalized medical solutions. However, gaps still exist in our understanding of sericin's mechanism of action, its long-term biocompatibility in humans, and the best methods for incorporating it into graft materials.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Min Keun Kim, Min Ji Ham, Won Rae Kim, Hyung Giun Kim, Kwang Jun Kwon, Seong Gon Kim, Young Wook Park
Summary: This study aimed to evaluate the accuracy of three-dimensional surgical guides and titanium implants in a rabbit model, as well as the surgical outcomes of mandibulectomy and reconstructive surgery. The accuracy of the implants was within the acceptable error range, while the surgical accuracy varied in different measured areas. The angle between the mandibular lower borders showed the most sensitive results, and the distance between the lingual cusps of the first molars represented the most accurate outcomes. The angle between the mandibular lower borders was found to be a good indicator for evaluating reconstructive surgery outcomes.
MAXILLOFACIAL PLASTIC AND RECONSTRUCTIVE SURGERY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Ji Hae Lee, HaeYong Kweon, Ji-Hyeon Oh, Seong-Gon Kim
Summary: This study evaluated the optimal scaffold material for silk sericin-based bone grafts using a critical-sized bone defect model in rat calvaria. The results showed that collagen exhibited better bone regeneration capability in silk sericin-based bone grafts compared to gelatin.
MAXILLOFACIAL PLASTIC AND RECONSTRUCTIVE SURGERY
(2023)
Article
Biochemistry & Molecular Biology
Ji Hae Lee, HaeYong Kweon, Ji-Hyeon Oh, Yei-Jin Kang, Dae-Won Kim, Won-Geun Yang, Weon-Sik Chae, Seong-Gon Kim
Summary: The study demonstrates that pretreatment with 4-hexylresorcinol (4HR) increases the β-sheet structure of silk sericin, induces BMP-2 expression, and enhances bone regeneration ability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Dentistry, Oral Surgery & Medicine
Yong-Chan Lee, Seong-Gon Kim
Summary: Orthognathic surgery has traditionally relied on traditional surgical planning, but the advent of virtual surgical planning (VSP) and 3D printing technologies has revolutionized the field, bringing unprecedented precision and customization. VSP allows for real-time adjustments and reduces planning time, while 3D printing enhances the predictability of surgical outcomes. The integration of VSP and 3D printing offers innovative solutions that surpass the limitations of traditional methods.
MAXILLOFACIAL PLASTIC AND RECONSTRUCTIVE SURGERY
(2023)
Article
Chemistry, Multidisciplinary
Dhouha Gaida, Young-Wook Park, Seong-Gon Kim
Summary: This study investigated the efficacy of 4-hexylresorcinol (4-HR) in mitigating taste bud apoptosis, specifically in relation to diabetes-induced taste issues. The findings highlight the significant deterioration of taste buds in diabetic rats and suggest the potential therapeutic benefits of 4-HR in improving taste perception.
APPLIED SCIENCES-BASEL
(2023)
Meeting Abstract
Cell & Tissue Engineering
Seong-Gon Kim, Ji-Hyeon Oh, Yei-Jin Kang, HaeYong Kweon
TISSUE ENGINEERING PART A
(2022)
Review
Dentistry, Oral Surgery & Medicine
Seong-Gon Kim
Summary: 4-Hexylresorcinol is an amphiphilic organic compound that regulates microorganisms by inducing stress on the endoplasmic reticulum and altering protein folding. It inhibits the NE-kappa B signal pathway and TNF-alpha production, while increasing the production of angiogenesis and bone formation related proteins. It also demonstrates strong anti-inflammatory reaction and capillary regeneration in diabetic models, making it applicable for various types of surgical wounds.
MAXILLOFACIAL PLASTIC AND RECONSTRUCTIVE SURGERY
(2022)
