Article
Medicine, Research & Experimental
Xianming Zhou, Chao Zhang, Fei Xie, Wei Wei, Rui Li, Qian Xu, Yu Wang, Philip A. Klenotic, Goutham Narla, Nianguo Dong, Zhiyong Lin
Summary: This study confirms the role of PP2A in the etiology of AAA and suggests that PP2A activation may represent a novel strategy for the treatment of abdominal aortic aneurysms.
Article
Cell Biology
Min Liu, Aiguo Liu, Jie Wang, Yansong Zhang, Yajuan Li, Ying Su, Alan Jian Zhu
Summary: Protein phosphatase V (PpV) is identified as a homeostatic regulator of Hh signaling, which competes with the catalytic subunit of PP2A for Wdb association to negatively regulate Wdb stability. This competition ensures graded Hh signaling through regulated Wdb stability. PpV functions as a Hh activity sensor to maintain Hh signaling homeostasis by regulating Wdb-mediated PP2A activity through feedback mechanisms.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Pedro Barbosa, Liudmila Zhaunova, Simona Debilio, Verdiana Steccanella, Van Kelly, Tony Ly, Hiroyuki Ohkura
Summary: Posttranslational modifications phosphorylation and ubiquitination cooperate to promote synaptonemal complex assembly, with the ubiquitin ligase complex SCF playing a key role by down-regulating the phosphatase subunit PP2A-B56. This regulation is mediated by F-box proteins Slmb/beta Trcp and Fbxo42, highlighting the importance of these proteins in maintaining genetic diversity during meiosis.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Liesbeth Guffens, Rita Derua, Veerle Janssens
Summary: Glioblastoma (GBM) is a common brain tumor with poor prognosis. This study investigated the role of PME-1, a protein phosphatase methylesterase, in GBM cell response to oxidative stress and the potential of targeting PME-1 for therapy. The results showed that PME-1 overexpression sensitized GBM cells to oxidative stress-induced cell death and activated MAPKAPK2-RIPK1 signaling. These findings suggest that GBM tumors overexpressing PME-1 may be more responsive to oxidative stress-inducing therapies.
CELL DEATH DISCOVERY
(2023)
Article
Oncology
Judit Domenech Omella, Emanuela E. Cortesi, Iris Verbinnen, Michiel Remmerie, Hanghang Wu, Francisco J. Cubero, Tania Roskams, Veerle Janssens
Summary: The deletion of Ppp2r5d gene accelerates the development of hepatocellular carcinoma (HCC) in a mouse model of DEN-induced hepatocarcinogenesis, suggesting its tumor-suppressive function. The deletion of Ppp2r5d also leads to the development of HCC and combined hepatocellular-cholangiocarcinoma (cHCC-CCA), and may regulate its own expression to restrict tumor growth.
Article
Biochemistry & Molecular Biology
Cinta M. Papke, Kali A. Smolen, Mark R. Swingle, Lauren Cressey, Richard A. Heng, Mourad Toporsian, Liyong Deng, Jacob Hagen, Yufeng Shen, Wendy K. Chung, Arminja N. Kettenbach, Richard E. Honkanen
Summary: Functional genomic approaches have advanced the discovery and understanding of rare genetic disorders, such as PPP2R5D-related disorder. The disorder, characterized by intellectual disability, hypotonia, autism-spectrum disorder, macrocephaly, and dysmorphic features, is caused by de novo single nucleotide changes in PPP2R5D. Research has shown that the E420K variant in PPP2R5D leads to altered signaling networks that can be regulated by rapamycin.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
E. Alan Salter, Andrzej Wierzbicki, Richard E. Honkanen, Mark R. Swingle
Summary: PP2A is a heterotrimeric holoenzyme composed of a common scaffold, a common catalytic subunit, and variable regulatory subunits. The interaction between one of the regulatory subunits and the catalytic subunit plays a critical role in the enzyme's catalytic function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Wen-Long Lei, Wei-Ping Qian, Qing-Yuan Sun
Summary: Meiosis is crucial for the continuation of life in sexually-reproducing organisms by producing haploid gametes. Errors in meiotic progression can lead to infertility and birth defects. Post-translational modifications, such as phosphorylation, play an important regulatory role in meiosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jade J. Dyson, Fatima Abbasi, Prajakta Varadkar, Brent McCright
Summary: Knockout experiments of B56 genes suggest that individual B56 genes are not essential for mouse survival, but simultaneous inactivation of B56 delta and B56 gamma can lead to impaired heart development. This indicates a strong genetic interaction between B56 delta and B56 gamma in regulating heart development.
Review
Biochemistry & Molecular Biology
Csaba Mathe, Csongor Freytag, Adrienn Kelemen, Marta M-Hamvas, Tamas Garda
Summary: This review summarizes the multiple regulatory mechanisms of protein phosphatase PP2A, including regulation of metabolic pathways, subcellular localization, cellular processes, and stress responses in plants. The findings contribute to a better understanding of plant cell functioning, potential agricultural applications, and insights into how vascular plants including crops cope with diverse environmental challenges.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Scott P. Lyons, Elora C. Greiner, Lauren E. Cressey, Mark E. Adamo, Arminja N. Kettenbach
Summary: The PPP family is responsible for cellular serine and threonine dephosphorylation, achieving substrate specificity and selectivity by forming multimeric holoenzymes. Methylation plays a crucial role in regulating PPP holoenzyme assembly for PP2A, PP4, and PP6, with implications in human diseases like cancer and neurodegeneration. Endogenous PPP methylation is shown to have a crucial regulatory function in transformed and non-transformed cell lines in an isoform-specific manner.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Sumati Hasani, Lyndsay E. A. Young, Warren Van Nort, Moumita Banerjee, Dylan R. Rivas, Jinhwan Kim, Xiaopeng Xiong, Ramon C. Sun, Matthew S. Gentry, Hiromi Sesaki, Tianyan Gao
Summary: Loss of Drp1 activates compensative metabolic pathways, glucose uptake and glycogenesis, to promote cell survival in colon cancer.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Karolina Pavic, Nikhil Gupta, Judit Domenech Omella, Rita Derua, Anna Aakula, Riikka Huhtaniemi, Juha A. Maatta, Nico Hofflin, Juha Okkeri, Zhizhi Wang, Otto Kauko, Roosa Varjus, Henrik Honkanen, Daniel Abankwa, Maja Kohn, Vesa P. Hytonen, Wenqing Xu, Jakob Nilsson, Rebecca Page, Veerle Janssens, Alexander Leitner, Jukka Westermarck
Summary: This study reveals the molecular level details and structural mechanisms of PP2A-B56 alpha inhibition by the oncoprotein CIP2A. CIP2A displaces the PP2A-A subunit and forms a pseudotrimer, blocking the B56 alpha substrate binding site and stabilizing CIP2A protein.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Patrick Partscht, Elmar Schiebel
Summary: CDC14 was initially identified as a crucial mediator of mitotic exit in budding yeast, belonging to the family of dual-specificity phosphatases (DUSPs) that are present in most eukaryotes. Contradicting data regarding the conservation of a cell cycle role in human paralogs CDC14A and CDC14B have sparked a contentious discussion, but redundancy may mask this role. Subsequent studies on CDC14A and CDC14B double knockouts in human and mouse have demonstrated that CDC14 activity is dispensable for mitotic progression in higher eukaryotes and instead suggested functional specialization.
Article
Multidisciplinary Sciences
Kathrin Jager, Juliane Mensch, Maria Elisabeth Grimmig, Bruno Neuner, Kerstin Gorzelniak, Seval Turkmen, Ilja Demuth, Alexander Hartmann, Christiane Hartmann, Felix Wittig, Anje Sporbert, Andreas Hermann, Georg Fuellen, Steffen Moller, Michael Walter
Summary: Telomeres are repetitive nucleotide sequences at the ends of chromosomes which can silence genes away from them to inhibit tumor growth. A study identified a set of highly conserved genes in replicatively aging animals and discovered that PPP2R2C gene, regulated by TPE-OLD, can suppress tumor-related signaling pathways.
Article
Biochemistry & Molecular Biology
Ilaria Ceglia, Christiane Reitz-, Jodi Gresack, Jung-Hyuck Ahn, Victor Bustos, Marina Bleck, Xiaozhu Zhang, Grant Martin, Sanford M. Simon, Angus C. Nairn, Paul Greengard, Yong Kim
Article
Biochemistry & Molecular Biology
Jung-Hyun Park, Du-Hyong Cho, Jee Young Lee, Hyeon-Ju Lee, Yena Ha, Jung-Hyuck Ahn, Inho Jo
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2015)
Article
Oncology
Hye Youn Sung, Jihye Han, Woong Ju, Jung-Hyuck Ahn
Article
Cell Biology
Ye-Ryung Kim, Giora Volpert, Kyong-Oh Shin, So-Yeon Kim, Sun-Hye Shin, Younghay Lee, Sun Hee Sung, Yong-Moon Lee, Jung-Hyuck Ahn, Yael Pewzner-Jung, Woo-Jae Park, Anthony H. Futerman, Joo-Won Park
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2017)
Article
Biochemistry & Molecular Biology
Jung-Hyun Park, Hye Youn Sung, Jee Young Lee, Hyun Jin Kim, Jung-Hyuck Ahn, Inho Jo
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2013)
Article
Gastroenterology & Hepatology
Joo Hyun Gil, Jeong Wan Seo, Min-Sun Cho, Jung-Hyuck Ahn, Hye Youn Sung
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
(2014)
Article
Oncology
Min Hee Kim, Joo-Won Park, Eun-Ji Lee, Shin Kim, Sun-Hye Shin, Jung-Hyuck Ahn, Yunjae Jung, Inkeun Park, Woo-Jae Park
Article
Multidisciplinary Sciences
Hye Youn Sung, Eun Nam Choi, Dahyun Lyu, Inhee Mook-Jung, Jung-Hyuck Ahn
Article
Multidisciplinary Sciences
Hye Youn Sung, Byung-Ok Choi, Jee Hyang Jeong, Kyoung Ae Kong, Jinha Hwang, Jung-Hyuck Ahn
Article
Biochemistry & Molecular Biology
Hyun-Jung Kim, Hye Jin Kim, Mi-Kyung Kim, Moon Kyoung Bae, Hye Youn Sung, Jung-Hyuck Ahn, Yun Hwan Kim, Seung Cheol Kim, Woong Ju
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Junghee Jin, Dionnet L. Bhatti, Ko-Woon Lee, Lucian Medrihan, Jia Cheng, Jing Wei, Ping Zhong, Zhen Yan, Cassandra Kooiker, Claire Song, Jung-Hyuck Ahn, Gerald J. Obermair, Amy Lee, Jodi Gresack, Paul Greengard, Yong Kim
MOLECULAR PSYCHIATRY
(2020)
Article
Biochemistry & Molecular Biology
Hye Youn Sung, Jihye Han, Yun Ju Chae, Woong Ju, Jihee Lee Kang, Ae Kyung Park, Jung-Hyuck Ahn
Summary: The mRNA expression of PARP4 is upregulated in cisplatin-resistant ovarian cancer cells, and this upregulation is associated with the hypomethylation of specific CpG sites on its promoter. Reduced PARP4 expression is related to cisplatin chemoresistance and its DNA methylation status may serve as a diagnostic biomarker for predicting response to cisplatin in ovarian cancer patients.
Article
Biochemistry & Molecular Biology
Hyunju Park, Jung A. Shin, Jiwoo Lim, Seulgi Lee, Jung-Hyuck Ahn, Jihee Lee Kang, Youn-Hee Choi
Summary: The expression of Cav-2 increases in the endothelial cells of the aging brain, promoting leukocyte infiltration and age-associated neuroinflammation. The number of iba-1-positive cells is reduced in the brains of aged Cav-2 knockout mice, and the levels of several chemokines are lower in the brains of young mice and aged Cav-2 KO mice. In addition, the expression of PECAM1 and VE-cadherin proteins is increased in the brains of old wild-type mice.
MOLECULES AND CELLS
(2022)
Article
Biochemistry & Molecular Biology
Hye Youn Sung, San-Duk Yang, Woong Ju, Jung-Hyuck Ahn
EXPERIMENTAL AND MOLECULAR MEDICINE
(2017)
Article
Clinical Neurology
Hye Youn Sung, Ji Yeoun Lee, Ae Kyung Park, Youn Joo Moon, Inho Jo, Eun-Mi Park, Kyu-Chang Wang, Ji Hoon Phi, Jung-Hyuck Ahn, Seung-Ki Kim