Journal
BMB REPORTS
Volume 42, Issue 12, Pages 817-822Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
DOI: 10.5483/BMBRep.2009.42.12.817
Keywords
Enzyme; Estivation; Metabolic rate depression; Protein phosphatase type-1; Purification
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Type-1 phosphatase (PP-1) was assessed in foot muscle (FM) and hepatopancreas (HP) of estivating (EST) Otala lactea. Snail PP-1 displayed several conserved traits, including sensitivity to inhibitors, substrate affinity, and reduction in size to a 39 kDa catalytic subunit (PP-1c). During EST, PP-1 activity in FM and HP crude extracts was reduced, though kinetics and protein levels of purified PP-1c isoforms were not altered. PP-1c protein levels increased and decreased in nuclear and glycogen-associated fractions, respectively, during EST. Gel filtration determined that a 257 kDa low K-m PP-1 alpha complex decreased during estivation whereas a 76 kDa high K-m complex increased in EST. Western blotting confirmed that the 76 kDa protein consisted of PP-1 alpha and nuclear inhibitor of PP-1 (NIPP-1). A suppression of PP-1 activity factors in the over-all metabolic rate depression in estivating snails and the mechanism is mediated through altered cellular localization and interaction with binding partners. [BMB reports 2009; 42(12): 817-822]
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