Journal
ANALYTICAL BIOCHEMISTRY
Volume 481, Issue -, Pages 1-3Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2015.04.013
Keywords
Capillary electrophoresis; DNase I footprinting; Fragment analysis; Single hit
Funding
- National Science Foundation [MCB-1051610]
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Fragment analysis was developed to determine the sizes of DNA fragments relative to size standards of known lengths using a capillary electrophoresis genetic analyzer. This approach has since been adapted for use in DNA footprinting. However, DNA footprinting requires accurate determination of both fragment length and intensity, imposing specific demands on the experimental design. Here we delineate essential considerations involved in optimizing the fragment analysis workflow for use in DNase I footprinting to ensure that changes in DNase I cleavage patterns may be reliably identified. (C) 2015 Elsevier Inc. All rights reserved.
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