Article
Biochemistry & Molecular Biology
Christina Z. Chung, Nileeka Balasuriya, Tarana Siddika, Mallory Frederick, Ilka U. Heinemann
Summary: In the cell, RNA abundance is regulated by transcription and decay rates; Gld2 controls mRNA and microRNA stability through adenylation; Gld2 activity is modulated by various factors, including post-translational modifications and interactions with RNA binding proteins.
Article
Biology
Charlene Estrada, Liliana Mirabal-Ortega, Laurence Mery, Florent Dingli, Laetitia Besse, Cedric Messaoudi, Damarys Loew, Celio Pouponnot, Corine Bertolotto, Alain Eychene, Sabine Druillennec
Summary: The MITF transcription factor directly interacts with RAF kinases, including ARAF, BRAF and CRAF, in melanoma cells. This interaction negatively regulates MITF activity by promoting its accumulation in the cytoplasm.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biology
Beata Machnicka, Aleksander Czogalla, Dzamila M. Boguslawska, Piotr Stasiak, Aleksander F. F. Sikorski
Summary: A new missense mutation in the ANK1 gene associated with hereditary spherocytosis phenotype is reported. This mutation, L1340P substitution, affects the conformation of the ankyrin ZZUD domain important for ankyrin binding to spectrin. The study shows the impact of this mutation on protein binding activity and cell morphology.
Article
Biochemistry & Molecular Biology
Junwon Kim, Mark D. Rose
Summary: In Saccharomyces cerevisiae, the p21-activated kinase Cla4p regulates polarized morphogenesis and cytokinesis. Cla4p kinase activity is down-regulated during mating, and this is achieved through phosphorylation of Cla4p at multiple sites, including Cla4p-S186, which reduces its kinase activity. Fus3p counteracts Cla4p kinase activity during mating by phosphorylating Fus2p at different sites to promote nuclear export and block import.
Article
Biochemistry & Molecular Biology
Sang-Ah Lee, Juyeon Lee, Kwanhyeong Kim, Hyunji Moon, Chanhyuk Min, Byeongjin Moon, Deokhwan Kim, Susumin Yang, Hyunjin Park, Gwangrog Lee, Raekil Park, Daeho Park
Summary: Phosphatidylserine (PS) plays a crucial role in cellular processes, and the peroxisomal protein Hsd17b4 is strongly associated with PS, with its Scp-2-like domain responsible for this association. The presence of PS in liposomes can disrupt this association, affecting the subcellular localization of Hsd17b4.
MOLECULES AND CELLS
(2021)
Article
Developmental Biology
Gabriela D'Amico, Isabelle Fernandez, Jesus Gomez-Escudero, Hyojin Kim, Eleni Maniati, Muhammad Syahmi Azman, Faraz K. Mardakheh, Bryan Serrels, Alan Serrels, Maddy Parsons, Anthony Squire, Graeme M. Birdsey, Anna M. Randi, Alfonso Bolado-Carrancio, Rathi Gangeswaran, Louise E. Reynolds, Natalia Bodrug, Yaohe Wang, Jun Wang, Pascal Meier, Kairbaan M. Hodivala-Dilke
Summary: Precise vascular patterning is dependent on the regulation of ERG transcription factor and DLL4/Notch signaling pathway. This study reveals that endothelial cell-specific focal adhesion kinase (FAK) controls ERG protein degradation, which in turn affects vascular development.
Article
Microbiology
Solene Denolly, Hongbo Guo, Miriam Martens, Anna Plaszczyca, Pietro Scaturro, Vibhu Prasad, Kessiri Kongmanas, Nuntaya Punyadee, Adisak Songjaeng, Dumrong Mairiang, Andreas Pichlmair, Panisadee Avirutnan, Ralf Bartenschlager
Summary: The widely used drug ivermectin inhibits the folding and secretion of luminal glycoproteins, including DENV NS1, by blocking the nuclear transport of transcription factors required for the expression of chaperones. This study reveals a novel mode of action of ivermectin and its potential application to other flaviviruses.
Article
Multidisciplinary Sciences
Oren Yakovian, Julia Sajman, Michal Alon, Rand Arafeh, Yardena Samuels, Eilon Sherman
Summary: NRas is a crucial mediator of the mitogenic pathway in both normal and cancer cells, and its signaling is regulated by dynamic interactions with functional clusters of BRAF or other related proteins at the plasma membrane.
Article
Engineering, Environmental
Xiaojin Zhang, Shijun Lin, Yichuan Wang, Fan Xia, Yu Dai
Summary: This study constructed cofactor-free organic nanozyme through self-assembly, which reduces the activation energy and increases the reaction rate. The catalysis of organic nanozyme mainly comes from the ordered assembly of peptide chains on the support surface and the assembly and disassembly caused by photoisomerization.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Medicine, Research & Experimental
Guo-Rong Chen, Yi-Bin Zhang, Shu-Fa Zheng, Ya-Wen Xu, Peng Lin, Huang-Cheng Shang-Guan, Yuan-Xiang Lin, De-Zhi Kang, Pei-Sen Yao
Summary: The majority of low-grade gliomas (LGGs) in adults progress to glioblastoma over time. This study analyzed the expression and prognosis of SPTBN2 in LGG and found that low expression of SPTBN2 is associated with unfavorable prognosis. In addition, several ncRNAs were identified that regulate SPTBN2 expression. Furthermore, SPTBN2 was found to have anti-tumor roles by modulating tumor immune infiltration and immune checkpoint expression.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Raul A. Salazar-Gonzalez, Mark A. Doll, David W. Hein
Summary: The acetylation status of NAT1 is an important factor that may play a relevant role in the progression of cancer. HDAC and Sirtuin inhibitors can modulate the acetylation status of NAT1 and affect its enzymatic activity. Knockdown of Sirt1 and Sirt2 genes also results in a reduction of NAT1 protein expression and catalytic activity.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alexey S. Kazakov, Alexander D. Sofin, Nadezhda V. Avkhacheva, Evgenia I. Deryusheva, Victoria A. Rastrygina, Maria E. Permyakova, Vladimir N. Uversky, Eugene A. Permyakov, Sergei E. Permyakov
Summary: Interferon-beta (IFN-beta) activity is affected by interactions with S100 proteins, including S100A1, S100A4, S100A6, S100P, and S100B. This study demonstrates the interaction between IFN-beta and S100B, and shows that monomerization of S100B enhances its affinity to IFN-beta. The modulation of IFN-beta activity by S100B could be relevant to the progression of various oncological and neurological diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Junmei Jiang, Jun Chen, Liting Luo, Lujie Wang, Hao Ouyang, Mingjian Ren, Xiangyang Li, Xin Xie
Summary: SGT1, an important plant disease resistance-related protein, was found to have a significant interaction with auxin (indole-3-acetic acid) in this study. The expression of SbSGT1 was significantly upregulated under different plant hormone treatments. These findings provide insights into the involvement of SbSGT1 in the auxin signal transduction pathway.
Article
Multidisciplinary Sciences
Yayoi Toyooka, Kazuhiro Aoki, Fumiko Matsukawa Usami, Sanae Oka, Azusa Kato, Toshihiko Fujimori
Summary: ERK is a serine/threonine kinase that regulates cellular events like cell proliferation and differentiation. The ERK signaling pathway is activated by fibroblast growth factors and is crucial for the differentiation of primitive endoderm cells in mouse embryos and embryonic stem cell culture. By using a biosensor called EKAREV-NLS, researchers discovered that ERK activity in undifferentiated and differentiating ESCs exhibits pulsatile dynamics, with some cells showing high-frequency ERK pulses and others showing no detectable pulses. Pharmacological inhibition experiments revealed the significance of Raf in determining the pattern of ERK pulses.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Kanagavel Deepankumar, Qi Guo, Harini Mohanram, Jessica Lim, Yuguang Mu, Konstantin Pervushin, Jing Yu, Ali Miserez
Summary: Dopa plays a central role in regulating the liquid-liquid phase separation (LLPS) of mussel foot proteins, facilitating the concentration of adhesive proteins at the plaque/substrate interface and enhancing the adhesive strength. This finding provides important insights for the biomanufacturing of protein-based materials such as bioadhesives and load-bearing fibers.
ADVANCED MATERIALS
(2022)
