Article
Biochemistry & Molecular Biology
Hanno Maassen, Leonie H. Venema, Marc G. Weiss, Tobias M. Huijink, H. Sijbrand Hofker, Anna K. Keller, Tom E. Mollnes, Marco Eijken, Soren E. Pischke, Bente Jespersen, Harry van Goor, Henri G. D. Leuvenink
Summary: Kidney extraction time has a detrimental effect on post-transplantation outcome. This study aimed to improve the flush-out and decrease ischemic injury by adding hydrogen sulphide (H2S) to the flush medium. The addition of H2S during flush out and static cold storage resulted in a reduction in pro-inflammatory cytokines without affecting renal function or injury markers.
Review
Biochemistry & Molecular Biology
Maria Irene Bellini, Francesco Tortorici, Maria Ida Amabile, Vito D'Andrea
Summary: Kidney transplantation is the preferred treatment for end-stage renal disease, with static cold storage being the standard preservation method. However, dynamic techniques like machine perfusion have shown potential to improve graft function and quality assessment, particularly in kidneys from extended criteria donors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Maria Abou Taka, George J. J. Dugbartey, Alp Sener
Summary: Renal transplantation is the preferred treatment for end-stage renal disease. The current gold standard for kidney preservation during transplantation is static cold storage at 4 degrees C. However, this method contributes to renal ischemia-reperfusion injury (IRI) which negatively affects graft survival and function. Recent studies focus on preserving kidneys at higher or subnormothermic temperatures to reduce the risk of cold renal IRI and enhance graft function and recipient survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Di Zhang, Yicun Wang, Song Zeng, Min Zhang, Xin Zhang, Yuxuan Wang, Zijian Zhang, Xi Wang, Xiaopeng Hu
Summary: By systematically screening gene expression profiles from over 1,000 specimens, 20 upregulated genes associated with RIRI in renal transplantation were successfully identified and validated, with 10 of them showing correlations with poor long-term transplant outcomes. Two newly identified genes, Btg2 and Rhob, were found to have crucial roles in RIRI and strong connections with NF-kappa B signaling.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Xinyu Yang, Di Lu, Rui Wang, Zhengxing Lian, Zuyuan Lin, Jianyong Zhuo, Hao Chen, Modan Yang, Winyen Tan, Mengfan Yang, Xuyong Wei, Qiang Wei, Shusen Zheng, Xiao Xu
Summary: Our study identified 11 different cell types in transplanted livers, with a highly complex ecosystem primarily shaped by myeloid-derived cell subsets that transition between different states and interact mutually. In transplanted steatotic livers, a pro-inflammatory phenotype of Kupffer cells (KCs) with high expression of colony-stimulating factor 3 (CSF3) was found to potentially contribute to fatty graft injury. Additionally, a subset of dendritic cells (DCs) with highly expressing XCR1 was correlated with CD8(+) T cells, mediating severer steatotic liver damage by I/R injury.
CELL PROLIFERATION
(2021)
Article
Surgery
Joana Ferrer-Fabrega, Emma Folch-Puy, Juan Jose Lozano, Pedro Ventura-Aguiar, Gabriel Cardenas, David Paredes, Angeles Garcia-Criado, Josep Antoni Bombi, Rocio Garcia-Perez, Miguel Angel Lopez-Boado, Ramon Rull, Enric Esmatjes, Maria Jose Ricart, Fritz Diekmann, Constantino Fondevila, Laureano Fernandez-Cruz, Josep Fuster, Juan Carlos Garcia-Valdecasas
Summary: This study retrospectively analyzed data from 380 pancreas transplants and compared the impact of different preservation solutions on transplant outcomes. The results showed that the IGL-1 solution had better performance in perioperative complications and higher survival rates at 1, 3, and 5 years compared to other solutions. Therefore, using the IGL-1 solution for perfusion and organ preservation may be a promising choice in clinical pancreas transplantation.
TRANSPLANT INTERNATIONAL
(2022)
Article
Surgery
Isabel M. A. Brueggenwirth, Matteo Mueller, Veerle A. Lantinga, Stefania Camagni, Riccardo De Carlis, Luciano De Carlis, Michele Colledan, Daniele Dondossola, Moritz Drefs, Janina Eden, Davide Ghinolfi, Dionysios Koliogiannis, Georg Lurje, Tommaso M. Manzia, Diethard Monbaliu, Paolo Muiesan, Damiano Patrono, Johann Pratschke, Renato Romagnoli, Michel Rayar, Federico Roma, Andrea Schlegel, Philipp Dutkowski, Robert J. Porte, Vincent E. de Meijer
Summary: Prolonged hypothermic oxygenated machine perfusion (HOPE) preservation of donor livers is safe and can lead to good outcomes in liver transplantation, with low incidence of complications and high survival rates. It can also reduce ischemia-reperfusion injury-related complications.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Review
Immunology
Jiale Li, Qinbao Peng, Ronghua Yang, Kunsheng Li, Peng Zhu, Yufeng Zhu, Pengyu Zhou, Gabor Szabo, Shaoyi Zheng
Summary: This review discusses the use of machine perfusion and mesenchymal stem cells to improve the quality and outcomes of marginal organs in transplantation. By continuously perfusing the organs to mimic physiological conditions, evaluating pre-transplant organ function, and treating the organs with pharmacological, gene, and stem cell therapy, the quality and adaptability of the organs can be enhanced. Promising preclinical results indicate the potential clinical translation of this innovative strategy to improve the quality of marginal grafts.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Thei S. Steenvoorden, Robert E. van Duin, Janneke A. J. Rood, Hessel Peters-Sengers, Azam S. Nurmohamed, Frederike J. Bemelman, Liffert Vogt, Joost W. van Der Heijden
Summary: This study aimed to evaluate the safety and feasibility of peri-procedural alkaline phosphatase (AP) administration in living donor kidney transplantation. The results showed that the bRESCAP treatment group had lower expression of injury biomarkers in urine after 7 days, but there was no significant difference in kidney function after 1 year. Therefore, the bRESCAP treatment is feasible in kidney transplantation, might have a dampening effect on ischemia-reperfusion injury-induced renal inflammation, and raises no safety concerns.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Adam Hosszu, Akos R. R. Toth, Tamas Lakat, Ganna Stepanova, Zsuzsanna Antal, Laszlo J. J. Wagner, Attila J. J. Szabo, Andrea Fekete
Summary: Kidney transplantation is the preferred treatment for end-stage kidney disease, and optimizing organ condition is crucial due to the shortage of transplantable organs. This study demonstrated that supplementing the preservation solution with the S1R agonist fluvoxamine reduced cold and warm ischemic injury, improved kidney function, mitigated histological injury, and reduced inflammation. The results suggest that S1R agonists have great potential in improving organ preservation and alleviating the problem of organ shortages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Wei Kelly Wu, Rei Ukita, Yatrik J. Patel, Michael Cortelli, Vincent Q. Trinh, Ioannis A. Ziogas, Sean A. Francois, Meredith Mentz, Nancy L. Cardwell, Jennifer R. Talackine, William M. Grogan, John W. Stokes, Youngmin A. A. Lee, Jinho Kim, Sophoclis P. Alexopoulos, Matthew Bacchetta
Summary: Xenogeneic cross-circulation technique has been successfully used to support and rehabilitate declined donor livers, resulting in preservation of organ structure and function, improved viability markers, and potential for further research in organ recovery and rehabilitation.
Review
Medicine, Research & Experimental
Aparna C. Swaminathan, Jamie L. Todd, Scott M. Palmer
Summary: Lung transplantation has substantially increased in volume in recent decades, with advances being made in understanding and treating post-transplant complications like CLAD. Ongoing studies are exploring novel approaches to improve long-term outcomes for lung transplant recipients.
ANNUAL REVIEW OF MEDICINE, VOL 72, 2021
(2021)
Article
Mechanics
Sttefan Ioan Campean, George Andrei Beschea, Maria Bianca Tabacaru, Alexandru Serban, Irinel Popescu, Florin Botea, Boris Rubinsky, Gabriel Nastase
Summary: This study aims to investigate the thermodynamic properties of Custodiol (R) at subfreezing temperatures and the metastable behavior of the solution, providing necessary data for using Custodiol (R) in isochoric systems for the preservation of biological matter.
Review
Cardiac & Cardiovascular Systems
Andrea Lechiancole, Sandro Sponga, Giovanni Benedetti, Arianna Semeraro, Giorgio Guzzi, Cristian Daffarra, Matteo Meneguzzi, Chiara Nalli, Daniela Piani, Marilyn Bressan, Ugolino Livi, Igor Vendramin
Summary: Heart transplantation is the best surgical treatment for end-stage heart failure patients, but the increasing age and high-risk factors of HTx candidates pose challenges. Donor organ preservation techniques, such as controlled hypothermia and machine perfusion, have shown potential in improving outcomes. However, the superiority of these techniques over traditional static cold storage is still debatable. This review aims to critically assess the available clinical data on donor heart preservation strategies for HTx.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Review
Medicine, General & Internal
Ruta Zulpaite, Povilas Miknevicius, Bettina Leber, Kestutis Strupas, Philipp Stiegler, Peter Schemmer
Summary: Kidney transplantation is the gold standard treatment for patients with end-stage kidney disease, but the quality of donor organs poses limitations. Ex-vivo machine perfusion technology has great potential in maintaining kidney viability and optimizing organ treatment, serving as an important component of novel techniques in transplant surgery.
FRONTIERS IN MEDICINE
(2021)
