☆ 4.6 Article

Chronic alcoholism associated with diabetes impairs erectile function in rats

BJU INTERNATIONAL (2010)

Journal

BJU INTERNATIONAL

Volume 105, Issue 11, Pages 1592-1597

Publisher

WILEY-BLACKWELL

DOI: 10.1111/j.1464-410X.2009.09084.x

Keywords

rats; diabetes mellitus; erectile dysfunction; chronic alcoholism

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Abstract

OBJECTIVE To investigate the effects of chronic ethanol consumption and diabetes on nitric oxide (NO)-mediated relaxation of cavernosal smooth muscle (CSM). MATERIAL AND METHODS Male Wistar rats were divided into four groups: control, isocaloric, diabetic and ethanol-diabetic. The CSMs were mounted in organ chambers for measurement of isometric tension. Contraction of the strips was induced by electrical field stimulation (EFS, 1-32 Hz) and phenylephrine. We also evaluated the effect of ethanol consumption on the relaxation induced by acetylcholine (ACh; 0.01-1000 mu mol/L), sodium nitroprusside (SNP, 0.01-1000 mu mol/L) or EFS (1-32 Hz) in strips pre-contracted with phenylephrine (10 mu mol/L). Immunoexpression of endothelial NO synthase (eNOS) and inducible NOS (iNOS) was also accessed. RESULTS The endothelium-dependent relaxation induced by ACh was decreased in CSM from ethanol-diabetic rats when compared with the controls, with a mean (sem) of 21 (4) vs 37 (2)%. Similarly, the potency and maximal responses induced by SNP were reduced in the ethanol-diabetic [3.97 (0.38) and 85 (1)%, respectively] and diabetic groups [3.78 (0.56) and 81 (2)%, respectively] when compared with the controls [5.3 (0.22) and 90 (3)%, respectively] and isocaloric [5.3 (0.19) and 92 (1)%, respectively] groups. Noradrenergic nerve-mediated contractions of CSM in response to EFS were increased in rats from ethanol-diabetic and diabetic groups when compared with the control and isocaloric groups. Conversely, there were no differences in EFS-induced relaxation among the groups. The immunostaining assays showed overexpression of eNOS and iNOS in the CSM from diabetic and ethanol-diabetic rats when compared with the control and isocaloric rats. CONCLUSION There was an impairment of relaxation of CSM from ethanol-diabetic and diabetic rats that involved a decrease in the NO-cyclic guanosine monophosphate signalling pathway by endothelium-dependent mechanisms accompanied by a change in the CSM contractile sensitivity.

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Mimosa pudica Linn. extract improves aphrodisiac performance in diabetes-induced male Wister rats

Chandrasekar Palanichamy, Damodar Nayak Ammunje, Parasuraman Pavadai, Sureshbabu Ram Kumar Pandian, Panneerselvam Theivendren, Shanmugampillai Jeyarajaguru Kabilan, Ewa Babkiewicz, Piotr Maszczyk, Selvaraj Kunjiappan

Summary: This study demonstrates the significant effects of Mimosa pudica Linn. extract on the male sexual system of rats with diabetes, including scavenging excessive free radicals and alleviating the deleterious effects of diabetes. The extract improves body weight, reproductive organ weight, sperm characteristics, and testes histocellular arrangement in rats. Additionally, it increases hormone levels and antioxidant enzymes while reducing oxidative stress markers in both serum and testicular homogenates. In silico molecular docking also predicts high potential compounds in the extract that show binding affinities with the PDE5 receptor. Overall, ethanolic extract of M. pudica L. exhibits significant aphrodisiac performance in diabetic rats.

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS (2023)