Journal
BIOTECHNOLOGY LETTERS
Volume 36, Issue 12, Pages 2489-2494Publisher
SPRINGER
DOI: 10.1007/s10529-014-1626-y
Keywords
Activity assay; Colo-205 cell line; Human Interleukin-22; Lactococcus lactis; NICE system; Synthetic construct
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Funding
- Programa de Apoyo a la Investigacion en Ciencia y Tecnologia (PAICYT) from the UANL
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Interleukin-22 (IL-22) participates in the modulation of innate immunity and inflammation. This cytokine has important therapeutic potential, such as with ulcerative colitis, liver and lung injury, and infection, in different animal models. We generated a Lactococcus lactis strain that secretes human IL-22 under the regulation of the nisin-inducible promoter. Identification and secretion of this cytokine was demonstrated using western blots of culture supernatants from IL-22-expressing bacteria. The recombinant IL-22 protein produced by L. lactis was biologically active as determined by its ability to induce IL-10 secretion when co-cultured with a colon epithelial cell line in vitro. We consider this novel strain a promising live vaccine for various therapeutic applications.
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