Journal
BIOTECHNOLOGY LETTERS
Volume 37, Issue 3, Pages 511-521Publisher
SPRINGER
DOI: 10.1007/s10529-014-1710-3
Keywords
Cell migration; Cell stiffness; Condition medium; CXC chemokine receptor 4; F-actin; Mesenchymal stem cells
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Funding
- National Natural Science Foundation of China [11032012, 11102240, 11272365]
- 111 Project [06023]
- Visiting Scholar Foundation of Key Laboratory of Biorheological Science and Technology (Chongqing University)
- Ministry of Education of China [CQKLBST-2012-008]
- Research Fund for the Doctoral Program of Higher Education of China [20130191110029]
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Interactions between tumors and mesenchymal stem cells (MSCs) can regulate cancer cell behavior and cancer progression. Rat bone marrow-derived MSCs (rMSCs) were isolated and purified by Percoll density gradient centrifugation. Conditioned media from rMSCs (MSC-CM) was prepared, and its role in cancer cell migration and the underlying molecular mechanism were investigated. MSC-CM increased the migration and up-regulated the expression of CXC chemokine receptor 4 (CXCR4) in rat hepatoma cells (CBRH-7919). F-actin remodeling was observed, and the Young's modulus was decreased in CBRH-7919 cells. A CXCR4 inhibitor suppressed the MSC-CM-induced CXCR4 expression and migration, restored the decrease in the Young's modulus and disrupted the formation of F-actin. MSC-CM thus promotes CBRH-7919 cell migration by lessening cell stiffness and increasing F-actin formation through up-regulation of CXCR4 expression. MSC-CM may therefore have a positive impact on cancer metastases and underlines a potential safety issue associated with clinical applications of MSCs.
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