Journal
BIOTECHNOLOGY LETTERS
Volume 33, Issue 11, Pages 2103-2110Publisher
SPRINGER
DOI: 10.1007/s10529-011-0686-5
Keywords
CHO cells; BlueSens analyzer; Oxygen uptake rate; Carbon dioxide production rate
Categories
Funding
- German State of Sachsen-Anhalt
Ask authors/readers for more resources
Batch-to-batch reproducibility of animal cell cultures can significantly be enhanced using process control procedures. Most informative signals for advanced process control can be derived from the volume fractions of oxygen and carbon dioxide in the vent line of the reactors. Here we employed simple low-cost sensors, previously not considered for off-gas analysis at a laboratory-scale cell cultures, and compared them with a simultaneously used quadrupole mass spectrometer, i.e., the standard equipment. A decisive advantage is that the sensors did not need any calibration and are easy to use. We show that monitoring and advanced control of cell cultures can significantly be simplified using the devices tested here and that the same batch-to-batch reproducibility can be obtained with much less effort than before.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available