Review
Food Science & Technology
Bruno Nicolau Paulino, Adones Sales, Lorena Felipe, Glaucia Maria Pastore, Gustavo Molina, Juliano Lemos Bicas
Summary: Aroma is a crucial attribute of food directly impacting consumer acceptance. In recent decades, interest in biotechnologically produced aroma compounds has grown as a sustainable way to provide natural additives for the food industry. Recent advances in microbial and enzymatic production of aroma compounds demonstrate their high potential for commercial applications.
CURRENT OPINION IN FOOD SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Shangjie Zhang, Feng Guo, Qiao Yang, Yujia Jiang, Shihui Yang, Jiangfeng Ma, Fengxue Xin, Tomohisa Hasunuma, Akihiko Kondo, Wenming Zhang, Min Jiang
Summary: In this study, the oleaginous yeast Yarrowia lipolytica was rationally engineered to utilize methanol and produce value-added chemicals like succinic acid. Through metabolic pathway engineering and gene regulation, the recombinant strain showed enhanced methanol utilization and cell growth. Transcriptome analysis revealed the rebalancing of methylotrophic metabolism. The study demonstrates the successful biological conversion of methanol into valuable chemicals.
Article
Biochemistry & Molecular Biology
Julio Cesar Soares Sales, Aline Machado de Castro, Bernardo Dias Ribeiro, Maria Alice Zarur Coelho
Summary: This study evaluated the depolymerization of PET by Y. lipolytica using two different strategies. The results indicate that this microorganism is capable of effectively degrading PET, and using YT medium in submerged cultures led to a higher conversion rate.
Review
Biotechnology & Applied Microbiology
Radin Shafierul Radin Yahaya, Yahaya M. Normi, Lai Yee Phang, Siti Aqlima Ahmad, Janna Ong Abdullah, Suriana Sabri
Summary: This article discusses the importance of keratinase and various strategies to increase its production, including cloning and expression in native hosts as well as molecular strategies in heterologous hosts. These strategies aim to meet the large-scale demand for recombinant keratinases.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biology
Luis E. Valencia, Matthew R. Incha, Matthias Schmidt, Allison N. Pearson, Mitchell G. Thompson, Jacob B. Roberts, Marina Mehling, Kevin Yin, Ning Sun, Asun Oka, Patrick M. Shih, Lars M. Blank, John Gladden, Jay D. Keasling
Summary: Researchers have successfully engineered P. putida to produce medium- and long-chain free fatty acids (FFAs) using functional genomics. They also developed a strategy to control the chain length of FFAs, resulting in a strain specialized in producing medium-chain FFAs. Furthermore, these strains were able to synthesize medium-chain fatty acid methyl esters, which have potential application as biodiesel blending agents.
COMMUNICATIONS BIOLOGY
(2022)
Article
Energy & Fuels
Yaojia He, Kai Li, Guangxu Bo, Jianhua Wang, Li Xu, Jinyong Yan, Min Yang, Yunjun Yan
Summary: This study investigates the effect of adding a deep eutectic solvent on the yield of biodiesel catalyzed by liquid lipase 2. The results show that the addition of the deep eutectic solvent can improve the yield of biodiesel and enhance the reusability of the enzyme.
Article
Microbiology
Yingli Zhang, Zhongchen Li, Li Li, Ben Rao, Lixin Ma, Yaping Wang
Summary: A method for rapid screening, expression, and purification of antimicrobial peptides (AMPs) was developed in this study. By using both prokaryotic and eukaryotic expression systems, multiple AMPs were successfully purified and shown to possess antibacterial activity.
Review
Biotechnology & Applied Microbiology
Shuobo Shi, Zhihui Wang, Lirong Shen, Han Xiao
Summary: This article introduces the application of synthetic biology in food production, including microbial production of food components and expanding the utilization of feedstocks. Synthetic biology offers potential solutions to high-quality food and the greenhouse effect.
TRENDS IN BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Jian Zha, Miaomiao Yuwen, Weidong Qian, Xia Wu
Summary: Xylose is the second most abundant sugar in lignocellulosic hydrolysates, and utilizing yeast strains for transforming xylose into valuable chemicals is a feasible and sustainable approach. Metabolically engineered yeasts have shown significant progress in producing natural products from xylose, such as aromatics, terpenoids, and flavonoids. Challenges and future perspectives in yeast engineering for commercial production of natural products using xylose as feedstocks are discussed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Karina Jasinska, Bartlomiej Zieniuk, Dorota Nowak, Agata Fabiszewska
Summary: The study evaluated the impact of freeze-drying on the extracellular lipases of Y. lipolytica KKP 379 and attempted to use the enzyme preparation in the synthesis of geranyl 4-hydroxyphenylpropanoate. The research confirmed that freeze-drying was effective in dehydrating yeast supernatant and improving enzyme activity. Additionally, the synthesized geranyl ester derivative showed antioxidant and antibacterial properties, demonstrating potential as a novel food additive.
Review
Biotechnology & Applied Microbiology
Jessica Lopes da Silva, Misael Bessa Sales, Viviane de Castro Bizerra, Millena Mara Rabelo Nobre, Ana Katia de Sousa Braz, Patrick da Silva Sousa, Antonio L. G. Cavalcante, Rafael L. F. Melo, Paulo Goncalves De Sousa, Francisco S. Neto, Aluisio Marques da Fonseca, Jose Cleiton Sousa dos Santos
Summary: This paper aims to present the recent advances in the biotechnological application of lipases Y. lipolytica, including their properties and efficient utilization in various industrial applications. The recent studies and progress in waste treatment, bioremediation, food sector, and biofuel production are highlighted. The USA and China, being major industrial powers, play a crucial role in the development and improvement of controlled systems for large-scale production of lipases from Y. lipolytica.
FERMENTATION-BASEL
(2023)
Article
Multidisciplinary Sciences
Anna Zimmermann, Julian E. Prieto-Vivas, Charlotte Cautereels, Anton Gorkovskiy, Jan Steensels, Yves Van de Peer, Kevin J. Verstrepen
Summary: CoMuTER is a new tool that utilizes the targetable helicase Cas3 and cytidine deaminase to induce mutations in large stretches of DNA, allowing for protein engineering and pathway optimization. It increases the number of mutations in the target region by 350-fold, with an average of 0.3 mutations per kilobase. We demonstrate its suitability for pathway optimization by doubling the production of lycopene in Saccharomyces cerevisiae after a single round of mutagenesis.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Li Li, Mengning Mei, Jun Wang, Jiang Huang, Xuyan Zong, Xiangyu Wang
Summary: In this study, three aflatoxin degrading enzyme genes were heterologously expressed in Pichia pastoris, and their enzyme activities and detoxification efficiency were detected. The results showed successful integration of the target genes into Pichia pastoris, with the expressed product effectively degrading AFB(1).
BIOTECHNOLOGY JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Katarzyna E. Kosiorowska, Xymena Polomska, Guokun Wang, Irina Borodina, Aleksandra M. Mironczuk
Summary: This study demonstrates that engineered strains of Y. lipolytica combined with metabolic engineering exhibit highly efficient biodegradation capabilities, especially showing stronger esterase activity under alkaline conditions.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2021)
Article
Energy & Fuels
Rui Xu, Zirong Chen, Yanyang Chen, Xiaoyue Wang, Yu Zhang, Xun Li, Fei Wang
Summary: This study successfully improved the expression level of Thermo myces lanuginosus lipase in Pichia pastoris using multiple strategies, achieving sustainable and economical enzymatic production of biodiesel.
Article
Engineering, Chemical
Hao Zhang, Shanliang Zheng, Lina Zheng, Feifei Lin, Bin Wang, Meng-Jiao Wang
Summary: This study investigated the volume change of natural rubber during stretching using hydrostatic weighing method and explored the differences among different carbon black compounds. Scanning electron microscope and X-ray diffraction were used to observe the cavity formation and the change of crystallinity.
POLYMER ENGINEERING AND SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Shuai Shao, Yu Zhang, Guantong Li, Zhenjun Yu, Yingying Cao, Lina Zheng, Kun Zhang, Xiaohui Han, Zhemin Shi, Hongmei Cui, Xiaomeng Song, Wei Hong, Tao Han
Summary: The study revealed that acute liver injury induced by CCl4 had three phases - early, progressive, and recovery - with different cell death patterns and regeneration dynamics. On the other hand, acute liver injury induced by D-gal/LPS only had early and progressive phases, leading to severe liver damage with high mortality. These findings provide valuable insights for clinical therapy and prognosis.
Article
Public, Environmental & Occupational Health
Shijun Wei, Belinda Johnson, Michael Breitenstein, Lina Zheng, John Snawder, Pramod Kulkarni
Summary: A method for aerosol chemical analysis using a handheld Raman spectrometer has been developed and applied to measure crystalline silica concentration in workplace atmosphere. The method involves collecting aerosol as a spot sample using a wearable optical aerosol monitor, and then quantitatively analyzing the spot sample for crystalline silica using a handheld Raman spectrometer.
ANNALS OF WORK EXPOSURES AND HEALTH
(2022)
Article
Chemistry, Physical
Li-Na Zheng, Liu-Yang Xu, Yang-Tian Yan, Tao Ding, Cheng-Cheng Feng
Summary: Two novel Cu(II) coordination polymers with different topological structures and magnetic behaviors were successfully synthesized by solvothermal method.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Biochemistry & Molecular Biology
Ying Liu, Bo Ding, Lina Zheng, Ping Xu, Zhiheng Liu, Zhao Chen, Peiyao Wu, Ying Zhao, Qian Pan, Yu Guo, Wei Wang, Wensheng Wei
Summary: Increasing evidence suggests that promoters and enhancers are closely associated with the 3D chromatin structure and have an impact on cellular functions. This study investigated the role of active promoters/enhancers that are predicted to form multiple 3D contacts with other active promoters/enhancers. The findings showed that these hotspots are essential for cell growth and survival, and their deletions result in changes in broad chromatin organization and the expression of distal genes. The essentiality of these hotspots is attributed to their association with multiple dysregulated non-essential genes that collectively affect cell fitness.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Richard Ainsworth, Deepa Hammaker, Gyrid Nygaard, Cecilia Ansalone, Camilla Machado, Kai Zhang, Lina Zheng, Lucy Carrillo, Andre Wildberg, Amanda Kuhs, Mattias N. D. Svensson, David L. Boyle, Gary S. Firestein, Wei Wang
Summary: This study characterizes clusters of RA cell lines with distinctive TF biology by integrating transcriptomic and epigenomic data and validates the predicted differences.
NATURE COMMUNICATIONS
(2022)
Article
Environmental Sciences
Zikang Feng, Lina Zheng, Lingyu Liu, Wenli Zhang
Summary: Particulates from diesel generator operation are a known air pollutant with adverse health effects. In this study, low-cost particulate matter sensors were used to monitor PM2.5 in a diesel generator plant. A data calibration algorithm based on a nonlinear SVM model was proposed to improve the measurement accuracy of the sensors. It was found that diesel generator operation, handling work, and human activity were the main sources of particulate matter, and dispersion was the main cause of increased PM2.5 concentrations in nonworking areas.
Article
Biochemistry & Molecular Biology
Shuai Shao, Yu Zhang, Feng Zhou, Xiaoxiang Meng, Zhenjun Yu, Guantong Li, Lina Zheng, Kun Zhang, Yuhan Li, Beichen Guo, Qi Liu, Mengxia Zhang, Xiaoxiao Du, Wei Hong, Tao Han
Summary: This study reveals that Airn alleviates acute liver injury by inhibiting hepatocyte apoptosis via inactivating the NF-kappa B signaling pathway. Airn could be a potential biomarker for acute liver injury.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2022)
Article
Chemistry, Inorganic & Nuclear
Dan Gao, Tao Ding, Wei-Wei Yan, Li-Na Zheng, Ke-Feng Xie, Zi-Wei Gao
Summary: When designing and synthesizing metal-organic frameworks (MOFs) with high stability and excellent adsorption/separation performance, the pore configuration and functional sites play a crucial role. In this study, two structurally similar cluster-based MOFs were reported, with different microporous environments but both exhibiting extremely high chemical stabilities and similar C2H2 adsorption capacities. One of the MOFs showed higher C2H2/CO2 selectivity under ambient conditions. A combination of breakthrough experiment and theoretical calculation was used to compare the gas adsorption and separation performance of the two MOFs, and the influence of the microporous environment on MOFs performance was further studied.
INORGANIC CHEMISTRY
(2022)
Article
Automation & Control Systems
Yini Wang, Lina Zheng, Sichun Wang
Summary: This paper studies a-rough fuzzy sets in information systems and proposes a novel decision-making method based on them. Firstly, the a-rough fuzzy sets in an expert information system are examined, and a preference index for decision problems based on a-rough fuzzy sets is proposed. Next, a decision-making method based on the preference degree function and the preference index is presented. Additionally, a decision-making algorithm based on information systems is introduced to determine the optimal choice. Finally, a numerical example is provided to demonstrate the feasibility of the proposed method, and a validity test is conducted.
INTERNATIONAL JOURNAL OF FUZZY SYSTEMS
(2023)
Article
Materials Science, Multidisciplinary
Zhanzhan Zheng, Pu Miao, Lina Zheng
Summary: This study proposes a Bayesian-based durability life prediction method for nano-modified concrete to address the low prediction accuracy of traditional methods. The diffusion function of CO2 in concrete is established using Fick's first law, and carbonation depth is determined through regression analysis. Additionally, a freeze-thaw cycle damage function for nano-modified concrete is established based on damage mechanics theory. Experimental results demonstrate high prediction accuracy for concrete durability life.
INTERNATIONAL JOURNAL OF MATERIALS & PRODUCT TECHNOLOGY
(2023)
Meeting Abstract
Clinical Neurology
Xinyi Leng, Xueyan Feng, Lina Zheng, Xuan Tian, Thomas W. Leung
Meeting Abstract
Clinical Neurology
Xuan Tian, Linfang Lan, Lina Zheng, Hui Fang, Yuming Xu, Thomas Leung, Xinyi Leng
Meeting Abstract
Clinical Neurology
Lina Zheng, Xuan Tian, Jill Abrigo, Xinyi Leng, Thomas Leung
Article
Biochemistry & Molecular Biology
Zhenjun Yu, Yuhan Li, Shuai Shao, Beichen Guo, Mengxia Zhang, Lina Zheng, Kun Zhang, Feng Zhou, Li Zhang, Chiyi Chen, Wentao Jiang, Wei Hong, Tao Han
Summary: Chinese researchers have discovered that a long non-coding RNA called Mir122hg plays a protective role in promoting the growth of new healthy cells after liver injury. By activating signaling pathways and promoting gene transcription, Mir122hg encourages hepatocyte proliferation, suggesting its potential use in new therapies.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
