Journal
BIOTECHNOLOGY LETTERS
Volume 32, Issue 8, Pages 1031-1038Publisher
SPRINGER
DOI: 10.1007/s10529-010-0258-0
Keywords
Bacterial infection; Gene expression; Grass carp; Quantitative real-time PCR; Reference gene
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Funding
- Science and Technology Key Project in Agricultural Areas of Guangdong Province [2008A020100016]
- Science and Technology Project of Ocean and Fisheries of Guangdong Province [A200899F01]
- Science and Technology Project of Guangzhou [20084411115, N0.2009J1-C021]
- Modern Agro-indursty Technology Research System [Nycytx-49-07]
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Expression of four reference genes of grass carp, including beta-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 18S rRNA (18S) and elongation factor-1 alpha (EF1 alpha), was studied in tissues of normal individuals and bacteria-infected individuals. EF1 alpha had the most stable expressions followed by 18S rRNA then GAPDH; ACTB had the least stability. After being infected with bacteria, the grass carp showed minimal changes in expression levels of EF1 alpha in the liver and head kidney, while ACTB had the most stable expressions in spleen but the least stable in liver. EF1 alpha is thus the optimal reference gene in quantitative real-time PCR analysis to quantitate the expression levels of target genes in tissues of grass carp.
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