Article
Environmental Sciences
Yixin Huang, Jinbin Lin, Jing Zou, Jiaxin Xu, Mengyun Wang, Huahua Cai, Baoling Yuan, Jun Ma
Summary: Introduction of ABTS as an electron shuttle in the HRP/H2O2 system enhanced the degradation efficiency of diclofenac under neutral pH conditions. The green-colored ABTS radical was identified as the main reactive species in the HRP/H2O2/ABTS system, showing no destruction and serving as an ideal electron shuttle. The reaction conditions including solution pH, HRP concentration, and H2O2 concentration impacted the formation of ABTS radical and the degradation of diclofenac in the system.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Food Science & Technology
Ming-Hsun Shen, Rakesh K. Singh
Summary: This study investigated the effect of H2O2 treatment on catalase-inactivated peanuts and found that it effectively reduced aflatoxins. Higher treatment temperature and catalase inactivation improved the efficacy of AF reduction. The oil quality of treated peanuts was not significantly affected. The weight loss and oil quality change of the treated peanuts were negligible. Peanuts were able to keep intact after the treatment.
Article
Spectroscopy
Furkan Burak Sen, Mustafa Bener, Burcu Bekdeser, Resat Apak
Summary: A redox-based colorimetric sensor was proposed for accurate determination of H2O2 in the presence of interferents, with two stages involving removal of antioxidants and determination of H2O2 content. The method showed comparable results to traditional peroxidase-based assays in various food and commercial products, and successfully detected peroxide-type explosives in the presence of antioxidants. The assay is simple, low-cost, insensitive to environmental agents, and not affected by turbidity or colored components in complex samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Applied
Anna Tama, Grzegorz Bartosz, Izabela Sadowska-Bartosz
Summary: This communication reveals that phenolic compounds commonly found in plant-based food and beverages can interfere with the Ampliflu Red-HRP assay used for quantification of hydrogen peroxide. Concentrations below 10 μM of catechin, propyl gallate, quercetin, and gallic acid were found to decrease the yield of the product, resorufin, by 50% in this system. The extent of inhibition increased with lower hydrogen peroxide concentrations. Therefore, careful interpretation of results is necessary when employing HRP methods for quantifying low concentrations of hydrogen peroxide in materials containing phenolic compounds.
Article
Nanoscience & Nanotechnology
Xixi Zhu, Yan Xue, Shutong Hou, Peng Song, Tao Wu, Hui Zhao, Nahid A. Osman, Abdullah K. Alanazi, Yan Gao, Hala M. Abo-Dief, Handong Li, Ben Bin Xu, Priyanka Wasnik, Qingyun Liu
Summary: The one-step hydrothermal reduction method was used to synthesize platinum nanoparticle-modified molybdenum disulfide-silica nanocomposites with excellent peroxidase-like activity. These nanocomposites showed the ability to decompose hydrogen peroxide into hydroxyl radicals and superoxide radicals, and were able to oxidize colorless 3,3',5,5'-tetramethylbenzidine into a blue oxidation product. A colorimetric sensing device for hydroquinone was developed using the nanocomposites, with a linear range of 0.5-9 μM and a low limit of detection. This sensing platform exhibited satisfactory sensitivity and selectivity in tap water and river water samples.
ADVANCED COMPOSITES AND HYBRID MATERIALS
(2023)
Article
Chemistry, Physical
Xixi Zhu, Hongyu Li, Tao Wu, Hui Zhao, Kaili Wu, Wenjing Xu, Fengjuan Qin, Wenxing Chen, Jinlong Zheng, Qingyun Liu
Summary: In this work, montmorillonite, a 2D layered mineral, was nano-decorated and used in biological detection. The anchored magnetic ferrite on the surface and interlayer of montmorillonite showed peroxidase-like activity, allowing for the establishment of a colorimetric sensing platform for H2O2. Coupled with cascaded catalytic reactions, a facile and efficient sensing platform for ACh was also established. This work highlights the competitive advantages of Co0.5Ni0.5Fe2O4-MMT in environmental assessment, biosensing, and disease monitoring.
Article
Green & Sustainable Science & Technology
Zhaojing Yu, Haiyang Cai, Shanshan Xiao, Weimin Zeng, Runlan Yu, Hong Cheng, Peiqi Cheng
Summary: After optimizing the VHP sterilization process, researchers studied the degradation kinetics of microbial EPS and investigated the changes of cell membrane peroxides and intracellular peroxidases. The key mechanism of short time sterilization using high concentration VHP was the serious damage to the cell membrane structure/function.
JOURNAL OF CLEANER PRODUCTION
(2023)
Review
Biochemistry & Molecular Biology
Lara Vogelsang, Karl -Josef Dietz
Summary: The temporal and spatial patterns of reactive oxygen species (ROS) in cells and tissues determine the plant's response to various stresses. Recent progress in dynamic cell imaging probes and sensitive proteomics has provided important information on the cellular redox state. Thiol peroxidases play a central role in this process.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Environmental Sciences
Jing-Jing Liu, Jong-Gook Kim, Hye-Bin Kim, Shakya Abeysinghe, Ying-Wu Lin, Kitae Baek
Summary: Enzyme-based biocatalytic treatment is an effective method for degrading organic pollutants. In this study, horseradish peroxidase (HRP) was immobilized on functionalized rice straw biochar using glutaraldehyde as a crosslinker. The biochar was pretreated by electrochemical and acid treatments to enhance its immobilizing ability.
Article
Chemistry, Analytical
Jing Wang, Wei Xu, Lin Zhou, Tianqi Zhang, Na Yang, Min Wang, Xuemei Luo, Lu Jin, Huaijun Zhu, Weihong Ge
Summary: The peroxidase-like activity of MXene@Fe3O4 nanocomposites and their colorimetric application were investigated, revealing higher activity and a ping-pong mechanism due to the synergetic impact of Fe3O4 and MXene phases. The nanocomposites showed promising results in catalyzing reactions and detecting hydrogen peroxide and glutathione, making them suitable for colorimetric sensors.
Article
Environmental Sciences
Mengyun Wang, Shiyi Qiu, Haoyu Yang, Yixin Huang, Lin Dai, Bilin Zhang, Jing Zou
Summary: A spectrophotometric method based on the reaction catalyzed by peroxidase (POD) and potassium iodide (KI) was developed for rapid measurement of hydrogen peroxide (H2O2) in aqueous solutions. The method showed good linear relationship and stability in various water samples, making it suitable for trace H2O2 analysis in rainwater and monitoring H2O2 concentration in different systems.
Article
Chemistry, Physical
Wei Lu, Shuhan Chen, Haifeng Zhang, Jiajun Qiu, Xuanyong Liu
Summary: In this study, Fe-N-C single atom nanozymes (SANs) with peroxidase and oxidase-like activities were prepared, and a colorimetric biosensor was developed for efficient detection of H2O2 and GSH.
JOURNAL OF MATERIOMICS
(2022)
Article
Environmental Sciences
Luis Angel Zambrano-Intriago, Celia G. Amorim, Alberto N. Araujo, Dmitrij Gritsok, Joan Manuel Rodriguez-Diaz, Maria C. B. S. M. Montenegro
Summary: This work describes the development of an affordable, simple, and accurate electrochemical biosensor for the determination of glyphosate (GLY) in river and drinking water samples. The biosensor showed high reproducibility, excellent analytical performance, repeatability, and accuracy. It proves to be selective against other pesticides, organic acids, and inorganic salts.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Applied
Pan Li, Sheng Zhang, Chenchen Xu, Lixiang Zhang, Qingqing Liu, Su Chu, Shuai Li, Guojiang Mao, Hua Wang
Summary: A simple and high-throughput colorimetric analysis array was constructed for quantifying H2O2 in milk. The use of capillary arrays and Fe3O4 quantum dots resulted in improved catalytic activity and environmental stability. The developed colorimetric platform can automatically fetch multiple samples, promising extensive applications in food safety monitoring.
Article
Biotechnology & Applied Microbiology
Derek J. Smith, Michelle A. Berry, Rose M. Cory, Thomas H. Johengen, George W. Kling, Timothy W. Davis, Gregory J. Dick
Summary: Cyanobacterial harmful algal blooms (CHABs) pose a global threat to freshwater ecosystems due to toxin production. This study reveals the key role of heterotrophic bacteria in decomposing hydrogen peroxide (H2O2) and suggests their activity may impact the fitness and strain composition of CHABs. The dichotomy of toxin-producing and non-toxin-producing strains may not drive the succession of toxin-producing species.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)