Article
Multidisciplinary Sciences
Samatha Bhat, Pachaiyappan Viswanathan, Shashank Chandanala, S. Jyothi Prasanna, Raviraja N. Seetharam
Summary: This study evaluated six different commercially available low serum/SFM and two different seeding densities to explore their ability to support the growth and expansion of BM-MSCs and assess the characteristics of BM-MSCs cultured in these media. Except for one of the SFM, all other media tested supported the growth of BM-MSCs at a low seeding density.
SCIENTIFIC REPORTS
(2021)
Article
Hematology
Yichao Dong, Ruikun Cai, Mingxia Fang, Yuqi Chen, Peng Li, Changlong Guo, Xu Ma
Summary: This study established a defined serum-free culture system for in vitro expansion of LT-HSCs, which successfully reconstituted the haematopoietic and immune systems in transplanted mice. The study also obtained the comprehensive expression profile and cellular components of LT-HSCs, providing a valuable tool for LT-HSC research.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Cell Biology
Bowen Zhang, Xumin Wu, Guicheng Zi, Lijuan He, Sihan Wang, Lin Chen, Zeng Fan, Xue Nan, Jiafei Xi, Wen Yue, Lei Wang, Liu Wang, Jie Hao, Xuetao Pei, Yanhua Li
Summary: By optimizing the culture protocol, a large number of megakaryocytes were successfully generated from human embryonic stem cells, confirming their biosafety and demonstrating their ability to form platelets. This study further enhances the clinical applications of megakaryocytes or platelets derived from pluripotent stem cells.
CELL PROLIFERATION
(2021)
Article
Engineering, Biomedical
Kyeong-Mo Koo, Young-Hyun Go, Seong-Min Kim, Chang-Dae Kim, Jeong Tae Do, Tae-Hyung Kim, Hyuk-Jin Cha
Summary: This study presents an electrochemical method for the label-free and non-destructive detection of naive embryonic stem cells (ESCs) derived from mouse ESCs, based on differences in cellular metabolism. By blocking glycolysis and oxidative phosphorylation, it was found that mitochondrial energy generation is the origin of the strong electrochemical signals of naive ESCs. The developed sensing platform can also sensitively identify mouse PSCs derived from MEFs among other cell types.
Article
Biochemistry & Molecular Biology
Seung-Bin Na, Bong-Jong Seo, Tae-Kyung Hong, Seung-Yeon Oh, Yean-Ju Hong, Jae-Hoon Song, Sang-Jun Uhm, Kwonho Hong, Jeong-Tae Do
Summary: This study investigated the impact of knocking out the key regulator of mitochondrial fission, the Dnm1l gene, on neural stem cells (NSCs). The results suggest that Dnm1l deficiency leads to impaired self-renewal ability and accelerated cellular aging in NSCs, possibly due to mitochondrial morphological and functional changes as well as increased inflammation and cellular stress response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Yunyan Sun, Ti-er Wang, Qianwen Hu, Wenxia Zhang, Yun Zeng, Xun Lai, Leisheng Zhang, Mingxia Shi
Summary: This study compared the biological characteristics and functions of hAMSCs preconditioned under serum-containing and serum-free conditions, revealing that hAMSCs in serum-free conditions showed preservation in certain aspects but had reduced proliferation and apoptosis, which could be rescued by PI3K-AKT-mTOR signaling reactivation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Agriculture, Dairy & Animal Science
Arpana Das, Dipak Bhuyan, Timothy Lalmalsawma, Partha Pratim Das, Simanta Koushik, Manmohan Singh Chauhan, Mandakranta Bhuyan
Summary: This study attempted to culture SSCs from Doom pig (Sus domesticus) using serum-free culture media with 5% Knockout Serum Replacement (KSR). The results showed that SSCs could be maintained in vitro for up to 30 days under serum-free culture conditions, which suggests the potential value of KSR as a protein source for improving livestock production and conservation.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper provides a thorough assessment of hormetic dose responses in embryonic stem cells, suggesting that this response may serve as a fundamental and highly conserved evolutionary strategy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Multidisciplinary Sciences
Delia Alba Soto, Micaela Navarro, Canbin Zheng, Michelle Margaret Halstead, Chuan Zhou, Carly Guiltinan, Jun Wu, Pablo Juan Ross
Summary: Through the use of a commercially available alternative and a chemically-defined substrate, we have successfully simplified the culture conditions for bESCs, resulting in newly derived bESC lines that are easy to establish, propagate, and maintain stability after long-term culture, while still retaining pluripotency.
SCIENTIFIC REPORTS
(2021)
Article
Cell & Tissue Engineering
Kyle B. Lupo, Jung-Il Moon, Andrea M. Chambers, Sandro Matosevic
Summary: This study explored the differentiation of NK cells from iPSCs under defined conditions and evaluated their immunotherapeutic potential against solid tumors. The results showed that iPSC-NK cells exhibited effector functions against tumor cells, suggesting their promising application in clinical adoptive transfer settings for immunotherapy of solid tumors.
Article
Cell Biology
Jun Ochiai, Larakaye Villanueva, Hope Niihara, Yutaka Niihara, Joan Oliva
Summary: This study developed a method to engineer multilayer adipose stem cell cell sheets (ASCCSs) using chemically defined/serum-free culture media, which can differentiate into osteoblasts and chondrocytes. The number of cells per cell sheet was estimated using cell sheet transmittance, providing important non-invasive information.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Ioannis J. Limnios, Yu-Qian Chau, Stuart J. Skabo, Denver C. Surrao, Helen C. O'Neill
Summary: This study developed a rapid and efficient method for producing retinal RPE cells from human embryonic stem cells (hESCs) using small molecules. The RPE cells exhibited key morphological, molecular, and functional characteristics of endogenous RPE, showing potential for clinical-grade cell production to treat AMD.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Younes F. Barooji, Kasper G. Hvid, Irene Isturiz Petitjean, Joshua M. Brickman, Lene B. Oddershede, Poul M. Bendix
Summary: The 3D actin cytoskeleton of naive mouse ESCs was investigated, revealing significant changes in peripheral actin structure and micro-rheological differences between cells cultured in different media. These results pave the way for linking physical properties and cytoskeletal architecture to cell morphology during early development.
Article
Cell & Tissue Engineering
Kohei Nagasaki, Ayumu Nakashima, Ryo Tamura, Naoki Ishiuchi, Kiyomasa Honda, Toshinori Ueno, Shigehiro Doi, Yukio Kato, Takao Masaki
Summary: This study compared the effects of serum-free cultured MSCs with MSCs cultured in medium containing fetal bovine serum on peritoneal fibrosis. The results showed that serum-free MSCs significantly suppressed fibrosis progression and inflammation, indicating their potential as a therapy for preventing peritoneal fibrotic progression. The in vitro experiments showed that although both types of MSCs suppressed TGF-beta 1 signaling, there was no significant difference between the two in their ability to do so.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Engineering, Biomedical
Bin Jiang, Weijie Li, Samantha Stewart, Wenquan Ou, Baolin Liu, Pierre Comizzoli, Xiaoming He
Summary: The study found that natural sand can seed ice above -10 degrees C, allowing hiPSCs to be cryopreserved without serum and with reduced cryoprotectants, leading to high cell survival. The cryopreserved hiPSCs also retained high pluripotency and functions.
BIOACTIVE MATERIALS
(2021)
Review
Biotechnology & Applied Microbiology
Miguel F. Tenreiro, Mariana A. Branco, Joao P. Cotovio, Joaquim M. S. Cabral, Tiago G. Fernandes, Maria Margarida Diogo
Summary: Human adult stem cells and patient-derived induced pluripotent stem cells are valuable tools for studying human biology, development, and disease. Organoids, which are organ-like structures created from stem cell derivatives, have revolutionized the field. However, there are still limitations such as variability, limited function, and small size. In this article, we summarize the latest technologies that aim to improve the biological fidelity and physiological relevance of organoids, and discuss their potential applications.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ana Fernandes-Platzgummer, Raquel Cunha, Sara Morini, Marta Carvalho, Juan Moreno-Cid, Carmen Garcia, Joaquim M. S. Cabral, Claudia L. da Silva
Summary: This study successfully establishes a serum-/xenogeneic-free microcarrier-based culture system for the cultivation of bone marrow-derived MSC and production of MSC-EVs. Large numbers of cells and EVs were obtained through static culture and continuous perfusion. The size and concentration of EVs were estimated using nanoparticle tracking analysis.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Review
Biochemistry & Molecular Biology
Mariana A. Branco, Tiago C. Nunes, Joaquim M. S. Cabral, Maria Margarida Diogo
Summary: It is now acknowledged that drug use during pregnancy is necessary to prevent or treat illnesses, but there is often a lack of information on teratogenic risk in humans. Animal models have been the main source of data, but their limitations in predicting human outcomes have led to misidentification of teratogenicity. The development of humanized in vitro models, particularly those that replicate important early developmental stages, can overcome these limitations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Chemical
Ricardo M. Silva, Sara Sousa Rosa, Raquel Cunha, Claudia L. da Silva, Ana M. Azevedo, Ana Fernandes-Platzgummer
Summary: Extracellular vesicles (EVs) are a cell-free strategy with broad biomedical applications. This study explores the purification of EVs from human mesenchymal stromal cell cultures using nuclease digestion and anion exchange chromatography. The results show that this purification platform is a viable alternative capable of delivering highly purified EVs for clinical trial testing.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Claudia Raquel Marques, Miguel de Almeida Fuzeta, Raquel Medina dos Santos Cunha, Joana Pereira-Sousa, Deolinda Silva, Jonas Campos, Andreia Teixeira-Castro, Rui Amandi Sousa, Ana Fernandes-Platzgummer, Claudia L. da Silva, Antonio Jose Salgado
Summary: Parkinson's disease is a neurodegenerative disorder characterized by the degeneration of dopamine neurons in the substantia nigra pars compacta. The presence of alpha-synuclein aggregates is considered a main contributor to the disease. Mesenchymal stromal cells have the potential to be used as a cell-free therapy for Parkinson's disease. However, there is a need to develop a protocol for large-scale production of the secretome under good manufacturing practices guidelines.
Article
Biochemistry & Molecular Biology
Laura Alves, Vanessa Machado, Joao Botelho, Jose Joao Mendes, Joaquim M. S. Cabral, Claudia L. da Silva, Marta S. Carvalho
Summary: Cell-based therapies using PDLSC may be a promising alternative to MSC(M) and MSC(AT) for periodontal regeneration, as PDLSC showed enhanced proliferative and osteogenic potential. PDLSC expressed osteogenic markers and had higher calcium content and upregulated osteogenic/periodontal genes compared to MSC(M) and MSC(AT). CD146 was specifically expressed by PDLSC, which also had a higher proliferative capacity. However, alkaline phosphatase activity did not increase in PDLSC.
Review
Biochemistry & Molecular Biology
Cristiana Ulpiano, Claudia L. da Silva, Gabriel A. Monteiro
Summary: Extracellular vesicles (EVs) are cell-derived lipid membranous structures that modulate cell-cell communication and have potential as drug delivery vehicles. Mesenchymal stromal cells (MSCs) secrete EVs which can be bioengineered to enhance their therapeutic potential. This review covers the fundamentals of EV biology, bioengineering strategies, and applications of bioengineered MSC-EVs, as well as the technical challenges to their clinical translation.
Article
Biochemistry & Molecular Biology
Frederico Barbosa, Fabio F. F. Garrudo, Ana C. Marques, Joaquim M. S. Cabral, Jorge Morgado, Frederico Castelo Ferreira, Joao C. Silva
Summary: This study aimed to develop electroconductive PAN/PEDOT:PSS nanofibers via electrospinning, which can mimic the fibrous extracellular matrix (ECM) of natural tissue and provide a platform for the delivery of exogenous electrical stimulation (ES). The resulting nanofibers were functionalized with apatite-like structures to imitate the inorganic phase of the bone ECM. The mineralized electroconductive nanofibers demonstrated improved biological performance, including enhanced proliferation of cells and up-regulated expression levels of bone marker genes, making them potential electroactive biomimetic scaffolds for bone defect repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Cell & Tissue Engineering
Ana Marote, Diogo J. Santos, Barbara Mendes-Pinheiro, Claudia Serre-Miranda, Filipa Antunes, Jonas Campos, Sandra I. Anjo, Ana Fernandes-Platzgummer, Claudia Lobato da Silva, Bruno Manadas, Margarida Correia-Neves, Laurent Roybon, Antonio J. Salgado
TISSUE ENGINEERING PART A
(2023)