Article
Microbiology
Jianhua Wang, Shanhai Lin, Guanghui Zhu, Zhiyong Zhao, Haoyu Wang, Miaoping Zhou, Xingming Zhao, Aibo Wu
Summary: This study reveals the molecular mechanisms of trichothecene biosynthesis in the Fusarium graminearum species complex under non-inducing conditions through transcriptome and proteome analysis. Differential gene expression and protein analysis of different strains identified novel genes and proteins associated with trichothecene biosynthesis.
Article
Biochemistry & Molecular Biology
Kazuyuki Maeda, Yuichi Nakajima, Yoshiaki Koizumi, Naoko Takahashi-Ando, Makoto Kimura, Shuichi Ohsato
Summary: The Fusarium graminearum species complex can produce different types of trichothecenes, which can slowly deacetylate into deoxynivalenol or nivalenol. The evolution of the FgTri1 gene has led to the emergence of new derivatives of trichothecenes known as NX-type trichothecenes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Laurie Taylor, Santiago Gutierrez, Susan P. McCormick, Matthew G. Bakker, Robert H. Proctor, Jennifer Teresi, Ben Kurtzman, Guixia Hao, Martha M. Vaughan
Summary: By emitting the signaling molecule trichodiene (TD), transgenic Trichoderma has enhanced biocontrol activity against Fusarium, reducing DON contamination. TD can also influence the expression of pathogenesis-related genes and has different effects on different wheat cultivars.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Abbot O. Oghenekaro, Maria A. Oviedo-Ludena, Mitra Serajazari, Xiben Wang, Maria A. Henriquez, Nancy G. Wenner, Gretchen A. Kuldau, Alireza Navabi, Hadley R. Kutcher, W. G. Dilantha Fernando
Summary: Fusarium graminearum is the main pathogen causing Fusarium head blight in wheat in Canada, with different population diversity and mycotoxin chemotype variations observed in different geographic regions. The 3ADON chemotype showed a significant increase in Saskatchewan and Manitoba over the past 15 years, while the 15ADON chemotype was predominant in Ontario and the United States. High gene diversity was found in all regions, with high gene flow between Saskatchewan and Manitoba but less gene flow among the western provinces and Ontario as well as the USA isolates. Bayesian clustering analysis revealed two clusters correlated with trichothecene types, showing more admixture among isolates of the 3ADON chemotype, potentially influencing the virulence of F. graminearum. Understanding these genetic and chemotypical variations will aid in developing FHB resistant wheat varieties and assessing mycotoxin risks in Canada.
Article
Engineering, Environmental
Shu-Ting Zhang, Tao Li, Shi-Kai Deng, Jim C. Spain, Ning-Yi Zhou
Summary: This study reveals a novel bacterial metabolic pathway for the degradation of 4-nitroanisole, initiated by a three-component cytochrome P450 system (pnaABC). This system exhibits broad substrate specificity and can catalyze the degradation of nitroanisole derivatives.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Plant Sciences
Fei Xu, Wei Liu, Yuli Song, Yilin Zhou, Xiangming Xu, Gongqiang Yang, Junmei Wang, Jiaojiao Zhang, Lulu Liu
Summary: The study in the main wheat production area of China found that climate factors, rather than cropping systems, had a greater impact on the relative prevalence of Fusarium graminearum and Fusarium asiaticum. Environmental conditions during the overwintering period appeared to be more important than those during other growth stages.
Article
Food Science & Technology
Lingqiao Chen, Junhua Yang, Haoyu Wang, Xianli Yang, Chengkang Zhang, Zhihui Zhao, Jianhua Wang
Summary: This review provides a comprehensive overview of the diagnostic techniques, distribution, frequency, structure elucidation, stability, toxicology, and molecular mechanisms of the emerging NX toxins produced by the NX-producing strains of the FGSC. The potential impacts of global warming on the production of these toxins are also discussed.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yanhui Lu, Qi Bai, Qiang Li, Xusong Zheng, Junce Tian, Jiawen Guo, Hongxing Xu, Zhongxian Lu
Summary: The study identified and characterized two cytochrome P450 monooxygenase genes, CsCYP6SN3 and CsCYP306A1, associated with the lethal effect of vetiver against C. suppressalis larvae. Inhibition of these genes significantly reduced pupation rate and pupa weight in the pests.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Narayanan Srividya, Iris Lange, Jana K. Richter, Matthias Wuest, B. Markus Lange
Summary: In this study, the enantioselectivity of multiple enzymes involved in monoterpenoid biosynthesis in peppermint and Japanese catnip was investigated. The experimental results showed that these enzymes exhibited different enantioselectivity towards intermediates of the pathway, and molecular modeling studies supported the experimental data. This study lays the groundwork for further structure-function studies on the origin of enantioselectivity in closely related species of the Lamiaceae.
Article
Plant Sciences
Fei Dong, Yunpeng Li, Xinyuan Chen, Jirong Wu, Shufang Wang, Xiao Zhang, Guizhen Ma, Yin-Won Lee, Mduduzi P. Mokoena, Ademola O. Olaniran, Jian Hong Xu, Jian Rong Shi
Summary: The study investigated the occurrence of FGSC in gramineous weeds near wheat fields in China, identifying Fusarium graminearum and Fusarium asiaticum as the dominant species. Different trichothecene genotypes and mycotoxins had varying effects on Fusarium head blight severity in wheat, highlighting the importance of weed management for reducing FHB inoculum.
Article
Plant Sciences
Andrew Muchlinski, Meirong Jia, Kira Tiedge, Jason S. Fell, Kyle A. Pelot, Lisl Chew, Danielle Davisson, Yuxuan Chen, Justin Siegel, John T. Lovell, Philipp Zerbe
Summary: This study reports the discovery of five cytochrome P450 monooxygenases in switchgrass, forming previously unknown furanoditerpenoids. Combinatorial pathway reconstruction showed that these enzymes catalyze furan ring addition directly. Transcriptional co-expression patterns and the presence of specific diterpenoids in roots support the occurrence of P450-derived furanoditerpenoids in planta.
Article
Agronomy
Vesna Zupunski, Radivoje Jevtic, Mirjana Lalosevic, Sanja Mikic, Branka Orbovic
Summary: In this study, species- and trichothecene-specific primers were used to monitor the F. graminearum species complex (FGSC) originating from Serbia. The primers were not specific for 11% of the F. graminearum population, but were effective in multilocus genotyping of Tri7, Tri3, and Tri5 genes. Alternaria spp. had a significant influence on grain infection with F. graminearum, indicating the need for further investigation.
Article
Biochemistry & Molecular Biology
Jiawei Liu, Hongmin Hu, Huimin Shen, Qingyin Tian, Wenjie Ding, Xiulian Yang, Lianggui Wang, Yuanzheng Yue
Summary: This study identified a total of 276 P450 genes belonging to 40 families in Osmanthus fragrans, suggesting their potential roles in fragrance synthesis. The research provides valuable insights into the functions of P450 genes and serves as a guide for further studies on promoting fragrance biosynthesis in ornamental plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Guixia Hao, Susan McCormick, Helene Tiley, Thomas Usgaard
Summary: The study demonstrates that transgenic Arabidopsis expressing FgTri101 can detoxify trichothecenes and excrete the acetylated toxins out of plant cells, providing plant protection.
Article
Plant Sciences
Hongsheng Gao, Huiqing Huang, Kaifeng Lu, Cuiting Wang, Xiaohua Liu, Zhizhong Song, Houjun Zhou, Lei Yang, Bei Li, Chunyan Yu, Hongxia Zhang
Summary: The expression of OsCYP714D1 gene improved salt tolerance in transgenic poplar plants by regulating gibberellin and ion homeostasis, resulting in increased plant height, K+ content, and decreased growth inhibition and Na+ content under high salt stress conditions. Transcriptomic analyses revealed up-regulation of GA biosynthesis, signaling, and stress responsive genes in transgenic plants, while gene ontology analyses indicated enrichment of genes involved in plant hormone and ion metabolic activities in the transgenic plants. OsCYP714D1 can be used as a candidate gene for engineering new tree varieties with improved biomass production and salt stress resistance.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Cell Biology
Yusuke Shiraishi, Tomohiko Maehama, Miki Nishio, Junji Otani, Hiroki Hikasa, Tak Wah Mak, Takehiko Sasaki, Teruki Honma, Yasumitsu Kondoh, Hiroyuki Osada, Minoru Yoshida, Masato Fujisawa, Akira Suzuki
Summary: DMPCA has been found to suppress the viability of BlC cells by inducing the phosphorylation of LATS1 and YAP1/TAZ, showing potential anti-tumor effects.
Editorial Material
Biotechnology & Applied Microbiology
Richard E. Lee, Minoru Yoshida
JOURNAL OF ANTIBIOTICS
(2022)
Article
Virology
Hirotaka Sato, Tomoyuki Murakami, Ryosuke Matsuura, Masako Abe, Seiji Matsuoka, Yoko Yashiroda, Minoru Yoshida, Hirofumi Akari, Yosuke Nagasawa, Masami Takei, Yoko Aida
Summary: Three compounds were found to inhibit the replication of HIV-1 in monocyte-derived macrophages, and one of them can directly bind to Vpr. These compounds have the potential to become new antiretroviral drugs for improving current HIV-1 therapy.
Article
Chemistry, Medicinal
Kosuke Hashimoto, Soichiro Ide, Mayumi Arata, Akiko Nakata, Akihiro Ito, Takashi K. Ito, Norio Kudo, Bangzhong Lin, Kazuto Nunomura, Keiko Tsuganezawa, Minoru Yoshida, Yasuo Nagaoka, Takaaki Sumiyoshi
Summary: In this study, a hybrid strategy combining HDAC6 inhibitors and brain-penetrant histamine H-1 receptor antagonists was used to design CNS-penetrant HDAC6 inhibitors. The identified compound KH-259 displayed selective inhibition and penetration into the brain, making it a potential therapeutic agent for neurodegenerative diseases.
ACS MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Yasir Sidiq, Daisuke Tamaoki, Takumi Nishiuchi
Summary: This study aimed to identify the candidates of regulatory proteins expressed in the plant epidermis that are related to the plant-pathogen interaction. Comparative proteomic studies were conducted on Arabidopsis leaf epidermis inoculated with fungal phytopathogen. The results showed that certain defense-related Arabidopsis proteins were rapidly induced in the epidermal tissues of inoculated leaves, while their expressions were not differentially regulated by fungal inoculation in the whole leaves.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Hiroki Kobayashi, Shohei Takase, Haruna Nishimura, Ken Matsumoto, Hironori Harada, Minoru Yoshida
Summary: To meet the energy and biosynthetic demands of cancer cells, they remodel their metabolism to increase glycolytic flux, known as the Warburg effect. However, this study found that decreased activity of the glycolytic enzyme phosphofructokinase-1 (PFK1) did not affect cancer cell survival or proliferation. This raises questions about the importance of PFK1 in cancer malignancy.
Editorial Material
Biochemistry & Molecular Biology
Minoru Yoshida, Seung Bum Park
CURRENT OPINION IN CHEMICAL BIOLOGY
(2023)
Editorial Material
Biotechnology & Applied Microbiology
Richard E. Lee, Minoru Yoshida
JOURNAL OF ANTIBIOTICS
(2023)
Article
Biochemistry & Molecular Biology
FangJing Li, Akio Ebihara, Yuri Sakahara, Sarara Matsumoto, Reina Ueno, WanXue Bao, Makoto Kimura, Shin-ichi Fuji, Masafumi Shimizu, Koji Kageyama, Haruhisa Suga
Summary: The S312T and F511S substitutions in the FfCYP51B gene have a synergistic effect on prochloraz resistance in Fusarium fujikuroi.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Shohei Takase, Takashi Hiroyama, Fumiyuki Shirai, Yuki Maemoto, Akiko Nakata, Mayumi Arata, Seiji Matsuoka, Takeshi Sonoda, Hideaki Niwa, Shin Sato, Takashi Umehara, Mikako Shirouzu, Yosuke Nishigaya, Tatsunobu Sumiya, Noriaki Hashimoto, Ryosuke Namie, Masaya Usui, Tomokazu Ohishi, Shun-ichi Ohba, Manabu Kawada, Yoshihiro Hayashi, Hironori Harada, Tokio Yamaguchi, Yoichi Shinkai, Yukio Nakamura, Minoru Yoshida, Akihiro Ito
Summary: Sickle cell disease (SCD) is caused by beta-globin gene mutations. G9a inhibitors, including RK-701, have been proposed as therapeutic agents for inducing fetal globin expression. This study describes the development of RK-701 as a selective and non-genotoxic G9a inhibitor, which upregulates BGLT3 long non-coding RNA and induces gamma-globin expression. BGLT3 is identified as an essential factor for gamma-globin induction and its universal role suggests its importance in SCD treatment.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Setsuko Komatsu, Kazuna Hamada, Takashi Furuya, Takumi Nishiuchi, Masahiko Tani
Summary: Proteomic analysis revealed that millimeter-wave irradiation affects membrane proteins in wheat roots, but the mechanism is not fully understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Maydelene Xiao Xuan Liew, Yuichi Nakajima, Kazuyuki Maeda, Naotsugu Kitamura, Makoto Kimura
Summary: Tri6 and Tri10 genes encode a transcription factor and a regulatory protein involved in trichothecene biosynthesis. The regulation of these genes is not well understood, especially in relation to culture medium pH and genetic factors. Changes in the trichothecene gene cluster core region also affect gene expression. This paper discusses the need for a revised understanding of the regulatory mechanism and proposes a model for Tri6 and Tri10 transcription.
FRONTIERS IN MICROBIOLOGY
(2023)
Editorial Material
Microbiology
Makoto Kimura, Hiromi Nishida, Masashi Kato, Masatoshi Goto, Tomoyuki Nakagawa
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ken-ichi Takayama, Seiji Matsuoka, Shungo Adachi, Teruki Honma, Masahito Yoshida, Takayuki Doi, Kazuo Shin-ya, Minoru Yoshida, Hiroyuki Osada, Satoshi Inoue
Summary: Various cellular activities are regulated by RNA-protein interactions, including lncRNAs. PSF interaction with its target RNAs is critical for hormone therapy resistance in prostate and breast cancers. A high-throughput screening system identified two promising compounds, N-3 and C-65, which inhibited cancer cell growth and induced apoptosis by inhibiting PSF-RNA interaction.
Article
Biochemistry & Molecular Biology
Saaya Sekine, Shohei Takase, Runa Hayase, Kota Noritsugu, Yuki Maemoto, Yasue Ichikawa, Kenji Ogawa, Yasumitsu Kondoh, Hiroyuki Osada, Minoru Yoshida, Akihiro Ito
Summary: In this study, NPD689 was identified as an inhibitor of the YAP-TEAD interaction. It suppressed the transcriptional activity of TEAD and reduced the viability of human malignant pleural mesothelioma and non-small cell lung cancer cells. These findings suggest that NPD689 could serve as a useful tool for studying the YAP-TEAD system and has potential as a cancer therapeutic agent.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)