Journal
BIOTECHNOLOGY LETTERS
Volume 31, Issue 2, Pages 221-226Publisher
SPRINGER
DOI: 10.1007/s10529-008-9852-9
Keywords
CO2; Escherichia coli; Gas pulsing; High cell density culture; Human-like collagen
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Funding
- National High Technology Research and Development Program of China [2006AA02Z246]
- National Natural Science Foundation of China [920776119]
- Northwest University for granting the Graduate Cross- discipline Funds [907YJC12]
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The evolution of CO2 in a fed-batch culture of recombinant Escherichia coli containing human-like collagen (HLC) cDNA was determined with an O-2-enriched air supply (40%, v/v) in a 12.8 l fermentor; a maximum CO2 concentration of 12.7% in the effluent gas was detected. The CO2 pulse injection experiments showed that: (1) a 20% CO2 pulse introduced in the batch cultivation phases inhibited cell growth but if introduced in the fed-batch cultivation phases slightly stimulated growth; and (2) CO2 inhibited HLC expression only in the expression phase, where the final HLC concentration decreased by 34% under a 3 h 20% CO2 pulse. The higher the CO2 concentration and/or the longer the duration of the CO2 pulse, the stronger the stimulatory or inhibitory effects.
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