Article
Engineering, Environmental
Ronghui Wu, Juyeol Bae, Hwisu Jeon, Taesung Kim
Summary: By studying regenerated protein actuators, we have successfully mimicked the humidity actuation of wild spider silks and regenerated abundant silkworm silk fibroin using microfluidic spinning technology. These regenerated silk fibers exhibit unique contraction properties when exposed to humidity or organic solvents and can be directionally aligned on hydrophobic substrates for easy folding and deformation. These actuators have a rapid response to humidity, high stability, and excellent repeatability.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Kenjiro Yazawa, Kosuke Hidaka, Jun Negishi
Summary: Silk-based materials, such as silk from silkworms and spiders, have potential applications in the medical field due to their mechanical toughness and low cytotoxicity. However, the scarcity and limitations in protein expression systems have hindered the utilization of spider silk. This study collected native spider silk directly from live spiders and investigated the cell adhesion behavior on different spider silk-based substrates. The findings contribute to promoting the use of spider silk in medical applications and sustainable development.
Article
Environmental Sciences
Natalia Bourguignon, Mauricio Alessandrello, Ross Booth, Constanza Belen Lobo, Maria Silvina Juarez Tomas, Luis Cumbal, Maximiliano Perez, Shekhar Bhansali, Marcela Ferrero, Betiana Lerner
Summary: This study used a microfluidic platform to explore the development, properties, and applications of biofilms in fluid flow, aiming to screen for biofilms capable of degrading PAHs. Computational fluid dynamics was used to optimize and evaluate the flow condition in microchannels, and the efficiency of PAHs removal was determined through HPLC analysis.
Article
Biochemistry & Molecular Biology
Vanessa T. Trossmann, Stefanie Heltmann-Meyer, Hanna Amouei, Harald Wajant, Raymund E. Horch, Dominik Steiner, Thomas Scheibel
Summary: Targeted therapies using biopharmaceuticals are clinically important in disease treatment. Protein-based therapeutics have limitations, which can be overcome using 3D constructs and producer cells. This study combines spider silk proteins and HEK293 producer cells to generate implantable 3D constructs for continuous release of targeted therapeutic proteins.
Article
Chemistry, Multidisciplinary
Yanbo Wang, Jiaxiong Zhu, Ao Chen, Xun Guo, Huilin Cui, Ze Chen, Yue Hou, Zhaodong Huang, Donghong Wang, Guojin Liang, Shan Cecilia Cao, Chunyi Zhi
Summary: A new binder for flexible lithium-ion batteries (LIBs) inspired by spider silk has been developed. The binder has super high elastic restorability and extraordinary adhesion, enabling stable cycling and voltage output even with significant electrode deformation. The flexible LIBs exhibit a significantly higher energy density than reported numbers.
ADVANCED MATERIALS
(2023)
Article
Biochemical Research Methods
Amanda E. Cherwin, Hayley N. Templeton, Alexis T. Ehrlich, Brielle H. Patlin, Charles S. Henry, Stuart A. Tobet
Summary: To protect the body from pathogens, the intestines have barriers made of collagens, disruptions to which are associated with various disorders. However, current models lack complexity to represent the biology that occurs at these barriers. In this study, a modified microfluidic device model was used to examine the effectiveness of collagen breakdown in causing barrier disruption, and promising results were obtained.
Article
Biochemical Research Methods
Amanda E. Cherwin, Hayley N. Templeton, Alexis T. Ehrlich, Brielle H. Patlin, Charles S. Henry, Stuart A. Tobet
Summary: To protect the body from external pathogens, the intestines have sophisticated epithelial and mucosal barriers. Collagens in the extracellular matrix play a critical role in maintaining the integrity of these barriers. However, current models lack the complexity to accurately represent the biology that occurs at these barriers. In this study, a modified microfluidic device model was used to examine the effectiveness of collagen breakdown in disrupting the intestinal barrier.
Article
Chemistry, Multidisciplinary
Zan Lamberger, Karolina Kocourkova, Antonin Minarik, Martin Humenik
Summary: A combination of self-assembly and photolithography is used to produce functionalized micropatterns. Spider silk proteins are covalently coupled to modified substrates and serve as seeds for the self-assembly of nanofibrils, resulting in immobilized networks. The micropatterns can be further functionalized with modified spider silk variants or nucleic acids.
ADVANCED MATERIALS INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Stanislav Pepeliaev, Matthias Torsten Ehebauer
Summary: This article presents a method for growing high-density Escherichia coli cultures in milliliter-scale bioreactors to produce milligram quantities of target protein. By utilizing a defined medium, a fed-batch feeding strategy at low temperature, and an advanced self-adapting control algorithm, a wet cell weight of 0.7 g and a protein yield of 1150 mg per liter final volume were achieved.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Saahil Sheth, Samuel Stealey, Nicole Y. Morgan, Silviya P. Zustiak
Summary: Hydrogel microspheres are widely used in biomedical applications, but the challenge lies in mixing precursor solutions for timed gelation systems. Through the design and evaluation of mixing devices, the serpentine geometry with a pillar array was found to be the most effective in fully mixing precursor solutions and enabling the fabrication of monodisperse microspheres.
Article
Chemistry, Physical
U. S. Jayapiriya, Prakash Rewatkar, Sanket Goel
Summary: This study established a simple, customized, and economical laser-induced graphene (LIG) material using a CO2 laser, integrated into a microfluidic device for Enzymatic Biofuel Cell (EBFC) application. The modified C-LIG electrodes showed improved performance and enzyme stability, generating higher power density compared to LIG electrodes. The novel platform provides a simple and quick fabrication method with potential for further optimization and improvements in power output.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Chemistry, Analytical
Xin Shi, Beibei Ma, Hongyu Chen, Wei Tan, Shiqing Ma, Guorui Zhu
Summary: This paper presents the development of a microfluidic/chitosan device for drug release to inhibit infections and guide bone regeneration. The device offers controlled drug release, flexible clinical use, low cost, and excellent antibacterial performance and biocompatibility.
Article
Chemistry, Applied
Ana Carolina Rafanhin Sousa, Cleyton Nascimento Makara, Lais Canniatti Brazaca, Emanuel Carrilho
Summary: In order to detect sulfonamides in milk, a simple and portable microfluidic paper-based analytical device (mu PAD) was developed for precise analysis without the need for pretreatment. This device has good linearity and selectivity, potentially allowing for on-site monitoring of milk quality to reduce adverse conditions associated with high concentrations of sulfonamides.
Article
Nanoscience & Nanotechnology
Sarah Lentz, Vanessa T. Trossmann, Christian B. Borkner, Vivien Beyersdorfer, Markus Rottmar, Thomas Scheibel
Summary: Improving the properties of biomaterials through engineered coatings is an area of increasing interest. Spider silk coatings have shown potential for explicit biomedical applications due to their excellent biocompatibility and mechanical properties.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Polymer Science
Elham Badali, Mahshid Hosseini, Negar Varaa, Narges Mahmoodi, Arash Goodarzi, Vajihe Taghdiri Nooshabadi, Sajad Hassanzadeh, Zohreh Arabpour, Mehdi Khanmohammadi
Summary: This study successfully developed cell-laden silk fibroin-phenol (SF-Ph) microparticles using a co-flow microfluidic system. The microparticles showed high cell survival rate and facilitated cellular proliferation, demonstrating their potential in tissue engineering and regenerative medicine applications.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Xenia Priebe, Manh Dat Hoang, Julian Ruediger, Maria Turgel, Julia Troendle, Wilfried Schwab, Dirk Weuster-Botz
Summary: This study provides a detailed analysis of whole-cell biotransformation of geraniol withEscherichia coliexpressing an UDP-glucose-dependent glucosyltransferase, serving as an optimal starting point for the design of other glycosylation processes.
BIOTECHNOLOGY LETTERS
(2021)
Article
Energy & Fuels
Torben Schaedler, Anna-Lena Thurn, Thomas Brueck, Dirk Weuster-Botz
Summary: This study investigated continuous lipid production with Microchloropsis salina in scalable TLC photobioreactors, demonstrating the scalability of the method and providing a TLC reactor model for realistic simulation of microalgae lipid production processes after re-identification of model parameters.
Article
Biotechnology & Applied Microbiology
Nikolas Von den Eichen, Lukas Bromig, Valeryia Sidarava, Hannah Marienberg, Dirk Weuster-Botz
Summary: The automation of parallel stirred-tank bioreactors allows for rapid optimization studies with other microbial production systems, reducing bioprocess development time and staff assignment considerably.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Microbiology
Anton Rueckel, Jens Hannemann, Carolin Maierhofer, Alexander Fuchs, Dirk Weuster-Botz
Summary: Syngas fermentation with acetogenic bacteria has shown promise in producing short- and medium-chain alcohols from CO-rich waste gases. However, the presence of impurities such as NH3, H2S, and NOx can inhibit microbial growth and product formation. NH3 and H2S had a positive effect on growth and alcohol production, while nitrate and nitrite reduced growth and alcohol concentrations. NOx components should be reduced or removed from syngas to optimize production processes.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
J. Wagner, D. Schafer, N. von den Eichen, C. Haimerl, S. Harth, M. Oreb, J. P. Benz, D. Weuster-Botz
Summary: This study successfully demonstrated the conversion of pectin in sugar beet press pulp to L-galactonate using engineered Saccharomyces cerevisiae. Through simple batch processes, the potential for sustainable production of high-value chemicals was shown.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Kristin Schoppel, Natalia Trachtmann, Fabian Mittermeier, Georg A. Sprenger, Dirk Weuster-Botz
Summary: The production of L-tryptophan from glycerol using Escherichia coli was analyzed through perturbation studies and metabolic control analysis. Insertion of a shikimate transporter into the genome enabled targeted perturbation within the product pathway, leading to significant changes in cell metabolism and chorismate biosynthesis. Metabolic control analysis identified controlling sites for L-tryptophan biosynthesis, with the addition of shikimate enhancing deviations in chorismate biosynthesis.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Computer Science, Software Engineering
Lukas Bromig, David Leiter, Alexandru-Virgil Mardale, Nikolas von den Eichen, Emmeran Bieringer, Dirk Weuster-Botz
Summary: The integration of laboratory devices into automated workflows is a challenging and time-consuming process, mainly due to the lack of interface standardization and the high cost of customized software solutions. The SiLA 2 Manager, based on the SiLA 2 standard, offers a lean and extensible framework for device discovery, management, and workflow design, bridging the gap between physical devices and higher-level software. It enables immediate control of SiLA 2 devices and user-friendly real-time data collection and storage.
Article
Microbiology
Luis Oliveira, Anton Rueckel, Lisa Nordgauer, Patric Schlumprecht, Elina Hutter, Dirk Weuster-Botz
Summary: Syngas fermentation has shown potential in producing fuels and platform chemicals, but the presence of impurities like NH3, H2S, and NOX affects its feasibility. This study investigated the effects of defined additions of NH4+, H2S, and NO3- on autotrophic batch processes with different acetogens. The addition of ammonium and nitrate inhibited cell growth and reduced alcohol concentrations, while H2S had a positive influence on one of the acetogens. Quantitative goals for syngas purification were identified, with a focus on NOX impurities, and maintaining acceptable concentrations of NH3 and H2S to avoid inhibition in the bioreactor.
Article
Biochemical Research Methods
Daniel Bischoff, Brigitte Walla, Dirk Weuster-Botz
Summary: Protein crystallization is a promising purification method, but monitoring the process remains challenging. Recent research shows that deep learning and synthetic data sets can be used to overcome these challenges and achieve excellent model performance.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Lukas Bromig, Dirk Weuster-Botz
Summary: Adaptive laboratory evolution (ALE) is a valuable tool for strain development, and a novel approach using a parallelized, L scale stirred-tank bioreactor system has been developed, which is faster and more efficient than traditional serial passaging and mL scale automated methods. Continuous estimation of biomass concentration and growth rate using off-gas analysis showed that the proposed method led to stable growth rates of E. coli with glycerol 9.4 times faster than traditional methods and 3.6 times faster than automated mL scale methods.
Article
Energy & Fuels
Ayse Koruyucu, Karlis Blums, Tillmann Peest, Laura Schmack-Rauscher, Thomas Brueck, Dirk Weuster-Botz
Summary: Microbial oil production from industrial waste streams and biogenic resources is a sustainable alternative to fossil and vegetable oils. However, the low product concentrations and high costs of fermentation and downstream processing pose challenges. In this study, high-cell-density yeast oil production with a defined medium was conducted on a large scale using a membrane bioreactor. By utilizing a semi-continuous mode, high lipid contents and yields were achieved, demonstrating the viability of using diluted sugar substrates for yeast oil production.
Article
Chemistry, Multidisciplinary
Philipp Benner, Finn Joshua Luedtke, Nina Beyer, Nikolas von den Eichen, Jose Enrique Oropeza Vargas, Dirk Weuster-Botz
Summary: This article presents an automated and scalable method for the high-throughput development of microalgae using photoautotrophic bioprocesses. The use of LED illumination modules and a liquid handling station enables individual light supply and control of pH and OD750 detection in parallel bioreactors. This method provides an efficient tool for the investigation and development of photoautotrophic processes with microalgae.
APPLIED SCIENCES-BASEL
(2023)
Review
Crystallography
Brigitte Walla, Daniel Bischoff, Iliana Corona Viramontes, Sergio Montes Figueredo, Dirk Weuster-Botz
Summary: Protein crystallization is widely used in academia and industry, especially in the pharmaceutical field. It is an attractive alternative to chromatography in downstream processing steps in biotechnology, food, and chemical industries. Monitoring protein crystallization is essential for understanding crystal growth mechanism and ensuring efficient process control. This review focuses on recent advances in monitoring techniques such as focused beam reflectance method (FBRM), machine learning-based image analysis, attenuated total reflectance Fourier transform infrared (ATR-FTIR), and UV/Vis spectroscopy.
Article
Microbiology
Miriam Baeumler, Veronika Burgmaier, Fabian Herrmann, Julian Mentges, Martina Schneider, Armin Ehrenreich, Wolfgang Liebl, Dirk Weuster-Botz
Summary: Syngas fermentation with clostridial co-cultures shows promise for CO conversion to alcohols. A study on C. kluyveri revealed reversible inhibition and chain elongation at different CO concentrations. A cascade system with two reactors was successfully established to produce C2-C6 alcohols. The continuous process showed improved alcohol yields compared to batch production.
Article
Biotechnology & Applied Microbiology
Anton Rueckel, Anne Oppelt, Philipp Leuter, Philipp Johne, Sebastian Fendt, Dirk Weuster-Botz
Summary: Synthesis gas fermentation is a promising microbial process for producing fuels and platform chemicals from renewable sources. This study investigated batch processes and continuous gassing with biogenic syngas, and found that both Clostridium carboxidivorans and Clostridium autoethanogenum showed good fermentation performance with biogenic syngas. However, achieving full carbon conversion to ethanol is still challenging.
FERMENTATION-BASEL
(2022)