Article
Energy & Fuels
Lakkana Laopaiboon, Suntaree Suporn, Preekamol Klanrit, Niphaphat Phukoetphim, Chalida Daengbussadee, Pattana Laopaiboon
Summary: Yeasts were isolated from potential sources and tested for ethanol tolerance and fermentation performance. Among 27 isolates, only five showed high ethanol tolerance and production efficiency, with SSJ01KKU performing the best under all conditions. Molecular taxonomic analysis revealed SSJ01KKU as S. cerevisiae and the other four isolates as Pichia caribbica.
Article
Biotechnology & Applied Microbiology
Taylor Reiter, Rachel Montpetit, Shelby Byer, Isadora Frias, Esmeralda Leon, Robert Viano, Michael Mcloughlin, Thomas Halligan, Desmon Hernandez, Ron Runnebaum, Ben Montpetit
Summary: An analysis of S. cerevisiae RC212 gene expression during Pinot noir fermentation at pilot scale (150 liters) using industry-relevant conditions revealed both common and diverse gene expression patterns. The reported gene expression patterns of RC212 are generally similar to those observed under laboratory fermentation conditions but also contain gene expression signatures related to yeast-environment interactions found in a production setting. Further research is needed to understand the roles of key genes and pathways highlighted in this study and their impact on industrial wine fermentation outcomes.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Taylor Reiter, Rachel Montpetit, Shelby Byer, Isadora Frias, Esmeralda Leon, Robert Viano, Michael Mcloughlin, Thomas Halligan, Desmon Hernandez, Rosa Figueroa-Balderas, Dario Cantu, Kerri Steenwerth, Ron Runnebaum, Ben Montpetit
Summary: Ribosomal DNA and RNA sequencing data from grape musts and primary fermentations inoculated with Saccharomyces cerevisiae RC212 show that fungal organisms detected in musts are not necessarily active during fermentation and do not significantly impact gene expression of the inoculated yeast strain. However, genetic signatures reflective of vineyard site and region were identified within the sequencing data, providing insights into environmental factors that may influence fermentation outcomes and wine characteristics. These findings contribute to understanding the complexity of vineyard-specific wine fermentation and offer starting points for further research on factors contributing to regional wine distinctiveness.
Review
Biotechnology & Applied Microbiology
Daniel Gomes, Mariana Cruz, Miriam de Resende, Eloizio Ribeiro, Jose Teixeira, Lucilia Domingues
Summary: The constant growth of the worldwide industry has raised concerns about greenhouse gas emissions, leading to efforts in transitioning to cleaner biotechnological processes. Bioethanol production, particularly through VHG fermentation, plays a significant role in improving process economics. Several obstacles remain, but potential improvements in fermentation organisms, culture media, and process conditions are being explored for future advancements in VHG technologies.
FERMENTATION-BASEL
(2021)
Article
Multidisciplinary Sciences
William M. Shaw, Lucie Studena, Kyler Roy, Piotr Hapeta, Nicholas S. McCarty, Alicia E. Graham, Tom Ellis, Rodrigo Ledesma-Amaro
Summary: CRISPR gene activation and inhibition is a powerful synthetic tool for influencing gene expression, with applications in foundational studies, cellular reprogramming, and metabolic engineering. The authors developed a method for inducible expression of multiple gRNAs, increasing the capacity for CRISPR gene activation and inhibition and enhancing flexibility in target gene manipulation. They also created a highly regulated and user-friendly CRISPR toolkit in yeast, enabling simultaneous activation and repression of 11 genes in central metabolism, resulting in a significant increase in succinic acid production.
NATURE COMMUNICATIONS
(2022)
Article
Agronomy
Heeyoung Park, Sang Un Park, Byeong-Kwan Jang, Jeong Jae Lee, Yong Suk Chung
Summary: Kenaf, an annual fiber crop mainly grown in India and China, is emerging as a promising bio-based energy source due to its rapid growth, CO2 absorption capabilities, and high productivity. The study evaluated 10 different cultivars of kenaf for cellulosic ethanol production, finding that ethanol yield and productivity were significantly impacted by cultivar type, rather than biomass composition. Engineering yeast to utilize xylose under pH control can maximize ethanol production from kenaf.
GLOBAL CHANGE BIOLOGY BIOENERGY
(2021)
Article
Agricultural Engineering
Ya Zhu Wang, Jin Zheng, Muhammad Nawaz, Fan Yang, Jiajun Hu, Min-Tian Gao
Summary: This study discovered that soluble polysaccharides from rice straw were conjugated with high levels of phenolic compounds. These conjugates significantly affected yeast growth and ethanol fermentation, improving their efficiency. The conjugates reduced osmotic stress and intracellular hydrogen peroxide levels, leading to higher yeast growth and ethanol production.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Multidisciplinary Sciences
Queency N. N. Okechukwu, Osman N. N. Kanwugu, Parise Adadi, Charles Odilichukwu R. Okpala, Elena G. G. Kovaleva
Summary: The potential of Chlorella vulgaris powder to improve the lifespan of Saccharomyces cerevisiae cultured in the presence of ethanol was studied. Results showed that 1% chlorella-supplemented media enhanced growth and ATP production significantly compared to control and 0.1% chlorella-supplemented sample.
JOURNAL OF TAIBAH UNIVERSITY FOR SCIENCE
(2023)
Article
Multidisciplinary Sciences
Sitong Chen, Zhaoxian Xu, Boning Ding, Yuwei Zhang, Shuangmei Liu, Chenggu Cai, Muzi Li, Bruce E. Dale, Mingjie Jin
Summary: This study utilized phylogeny-guided big data mining, rational modification, and ancestral sequence reconstruction strategies to discover 13 new active xylose isomerases (XIs) for Saccharomyces cerevisiae, demonstrating the importance of the amino-terminal fragment for maintaining basic XI activity. These XIs were used to construct four efficient xylose-utilizing S. cerevisiae strains, and the strain S. cerevisiae CRD5HS achieved ethanol titers as high as 85.95 and 94.76 g/liter from pretreated corn stover and corn cob, respectively, without detoxifying or washing pretreated biomass. The genetic regulation mechanisms obtained from adaptive laboratory evolution provide practical references for mining other scarce and valuable enzymes.
Article
Food Science & Technology
Jitka Laurent, Nore Struyf, An Bautil, Albina Bakeeva, Mikolaj Chmielarz, Marika Lyly, Beatriz Herrera-Malaver, Volkmar Passoth, Kevin J. Verstrepen, Christophe M. Courtin
Summary: A diet low in FODMAPs can help reduce symptoms in patients with irritable bowel syndrome, but avoiding cereal grain products may lead to nutritional deficiencies. Developing low-FODMAP, high-fiber cereal grain products using K. marxianus in bread production can effectively reduce fructan levels and maintain fructan and fructose levels below the threshold for low-FODMAP products.
FOOD AND BIOPROCESS TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Zhilong Lu, Ling Guo, Xiaoling Chen, Qi Lu, Yanling Wu, Dong Chen, Renzhi Wu, Ying Chen
Summary: By analyzing the genomic structure and gene expression changes of a mutant S. cerevisiae strain, researchers found that the mutant strain exhibited improved ethanol fermentation capacity. The changed genes were mainly enriched in pathways related to carbohydrate metabolism, amino acid metabolism, cofactors and vitamins metabolism, and lipid metabolism. Furthermore, protein interaction analysis identified key hub genes that connected these pathways.
FERMENTATION-BASEL
(2023)
Article
Chemistry, Applied
Hideaki Tokuyama, Ryo Aoyagi, Kazuto Fujita, Yuki Maekawa, Shohei Riya
Summary: Ethanol fermentation using a hybrid system containing free and immobilized yeast cells in macroporous hydrogel scaffold was studied, with immobilization achieved through NIPA and MTGA hydrogels. The hybrid system showed higher ethanol productivity than the control system, with macroporous hydrogel allowing stable growth of immobilized yeast cells. In steady-state fermentation, the hybrid system had significantly higher cell densities and ethanol productivities compared to the control system.
REACTIVE & FUNCTIONAL POLYMERS
(2021)
Article
Biotechnology & Applied Microbiology
Bin Zhang, Mesfin Geberekidan, Zhao Yan, Xia Yi, Jie Bao
Summary: A highly thermotolerant strain of Saccharomyces cerevisiae, S. cerevisiae Z100, was obtained through laboratory adaptive evolution. The cell survival ratio of S. cerevisiae Z100 at 50 degrees C increased by 1.2 times compared to the parental strain, resulting in higher ethanol production during cellulosic ethanol fermentation. Transcriptome analysis suggested that enhanced trehalose and glycogen synthesis, as well as carbon metabolism, contributed to the improved thermotolerance and ethanol fermentability of S. cerevisiae Z100.
FERMENTATION-BASEL
(2023)
Review
Biotechnology & Applied Microbiology
Juhi Sharma, Vinod Kumar, Rajendra Prasad, Naseem A. Gaur
Summary: Lignocellulosic biomass is an abundant material on earth and can be processed by microorganisms to produce biofuels. Saccharomyces cerevisiae is an ideal candidate for consolidated bio-processing (CBP) to produce cellulose-based ethanol. However, there are still challenges in the process, such as incomplete substrate catabolism and low expression of heterologous proteins.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Biochemistry & Molecular Biology
Maria Mavrommati, Seraphim Papanikolaou, George Aggelis
Summary: This study describes an Adaptive Laboratory Evolution (ALE) strategy to develop Saccharomyces cerevisiae populations with increased tolerance to ethanol. The evolved populations after 100 generations could survive for 1 hour at 23% v/v ethanol and for 2 hours at 25% v/v ethanol. The fermentation ability and growth rate of the yeast strains were significantly improved through ethanol-based evolution.
PROCESS BIOCHEMISTRY
(2023)
Article
Energy & Fuels
Tian-Jie Ao, Kai Li, Muhammad Aamer Mehmood, Xin-Qing Zhao, Feng-Wu Bai, Raj Boopathy, Chen-Guang Liu
Summary: This study utilized deep eutectic solvent (DES) for pretreating corn stover to improve the efficiency of subsequent saccharification. The optimal conditions were determined using response surface methodology, and it was found that a molar ratio of lactic acid (LA) to choline chloride (ChCl) of 1 at 140 degrees C for 4 hours achieved the highest cellulose and hemicellulose digestibility as well as delignification efficiency. The study also revealed the formation of humins at higher temperatures, which hindered enzymatic accessibility.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biotechnology & Applied Microbiology
Hong-Qi Chen, Qi Xing, Cheng Cheng, Ming-Ming Zhang, Chen-Guang Liu, Verawat Champreda, Xin-Qing Zhao
Summary: The addition of zinc sulfate improves the tolerance of Saccharomyces cerevisiae to acetic acid and mixed inhibitors, leading to higher ethanol productivity. Overexpression of KIC1 and CDC42 genes enhances the growth and ethanol fermentation ability of yeast under stress conditions, along with significant upregulation of stress response genes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Energy & Fuels
Muhammad Nabeel Haider, Aqib Zafar Khan, Muhammad Usman, Deepanraj Balakrishnan, Muhammad Rizwan Javed, Sana Malik, Chen-Guang Liu, Muhammad Aamer Mehmood, Ghulam Abbas Ashraf
Summary: This study evaluated the resource recovery, wastewater treatment, and biomass production potential of a filamentous cyanobacterium Plectonema terebrans BERC10 using an outdoor open-pond cultivation system. The results showed that seasonal and nutrient variations had a strong impact on metabolites and biomass production. The strain showed the highest biomass production and carbohydrate content in the summer, and the highest protein content in the winter. Additionally, the strain demonstrated promising potential for wastewater treatment by reducing nitrogen, phosphorus, dissolved solids, COD, and BOD levels.
Article
Chemistry, Multidisciplinary
Kai Li, Jia-Wei Zhang, Feng Li, Xin-Qing Zhao, Feng-Wu Bai, Hao Song, Chen-Guang Liu
Summary: This study investigated ethanol fermentation by Zymomonas mobilis in an electro-fermentation system, which increased the ethanol titer by 12.8%. The underlying mechanism was revealed by exploring the correlation among intracellular redox parameters and transcriptome analysis. Two strategies to enhance the system were proposed, including the supplementation of electron shuttles and the manipulation of essential electricity-sensing genes.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Guilherme de Souza Hassemer, Lucas Henrique do Nascimento, Yen-Han Lin, Clarice Steffens, Alexander Junges, Eunice Valduga
Summary: The present study evaluated the impact of oxidation-reduction potential (ORP) on the production of poly(3-hydroxybutyrate) (P(3HB)) by Bacillus megaterium. ORP levels can reassign the cell's metabolic flux, affecting enzyme expression and improving control over the fermentative process. The experiments were conducted using a fermentation vessel and an ORP probe, with different ORP values tested to determine their effects on biomass and polymer production. Results showed that ORP levels greatly influenced the metabolism of B. megaterium cells, and measurement and control of ORP could be valuable in maximizing polymer production under different culture conditions.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Article
Agricultural Engineering
Xiao-Yan Huang, Tian-Jie Ao, Xue Zhang, Kai Li, Xin-Qing Zhao, Verawat Champreda, Weerawat Runguphan, Chularat Sakdaronnarong, Chen-Guang Liu, Feng-Wu Bai
Summary: Building accurate machine learning models can promote biorefinery. This study proposed a strategy to enhance the generalization ability of models and overcome data insufficiency for mixed sugar fermentation simulation. By using multiple inputs single output models and consensus yeast model, the accuracy of the models can be improved.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Kai Li, Cheng Li, Xin-Qing Zhao, Chen-Guang Liu, Feng-Wu Bai
Summary: Corynebacterium glutamicum was engineered to efficiently produce succinic acid from corn stover pre-treated by concentrated-alkali under steam-assistant conditions. The engineered strain achieved high succinate production by blocking by-products pathways, enhancing carbon flux, and releasing end-product inhibition. Xylose utilization pathways were also introduced into the strain for fully utilizing xylose in lignocellulosic hydrolysate. The combined use of glucose and xylose yielded 64.16 g/L of succinic acid from CASA-pretreated corn stover.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Environmental
Azeem Asghar, Chen-Guang Liu, Imtiaz Ali, Aqib Zafar Khan, Hui Zhu, Ning Wang, Muhammad Nawaz, Tanveer A. Tabish, Muhammad Aamer Mehmood, Raqiqa Tur Rasool
Summary: This study found that Saccharum bengalense has high biomass energy value and low sulfur content, making it suitable for pyrolysis reactions to produce various chemicals and energy.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Yang Tian, Ning Wan, Hanqing Zhang, Chang Shao, Ming Ding, Qiuyu Bao, Haiyang Hu, Huiyong Sun, Chenguang Liu, Kun Zhou, Shuai Chen, Guangji Wang, Hui Ye, Haiping Hao
Summary: Hyperactivated glycolysis is a metabolic characteristic of cancer cells, and glycolytic metabolites have nonmetabolic functions as signaling molecules. However, how these metabolites interact with their targets remains unclear. This study introduces a new approach called TRAP to identify target candidates and interactions of glycolytic metabolites in cancer cells, revealing diverse regulatory modalities and potential for cancer therapy.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Lian-Ying Cao, Chen-Guang Liu, Shi-Hui Yang, Feng-Wu Bai
Summary: In this study, a novel strategy was developed to enhance the stress tolerance of Z. mobilis by regulating biofilm formation through heterologous expression of pfs and luxS genes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Kittapong Sae-Tang, Pornsiri Bumrungtham, Wuttichai Mhuantong, Verawat Champreda, Sutipa Tanapongpipat, Xin-Qing Zhao, Chen-Guang Liu, Weerawat Runguphan
Summary: In this study, the tolerance to d-lactic acid and production were improved in Pichia pastoris by introducing a flocculation gene from Saccharomyces cerevisiae (ScFLO1) and a d-lactate dehydrogenase gene from Leuconostoc pseudomesenteroides (LpDLDH). The engineered strain KM71-ScFlo1-LpDLDH achieved a d-lactic acid titer of 5.12 +/- 0.35 g/L in 48 h, which was 2.6-fold higher than the control strain. Transcriptomics analysis revealed the upregulations of genes involved in lactate transport and iron metabolism as the mechanism of increased tolerance to d-lactic acid.
Article
Biotechnology & Applied Microbiology
Ning Wang, Puyu Zhang, Xiaoli Zhou, Jia Zheng, Yi Ma, Chenguang Liu, Tao Wu, Hong Li, Xiaoqin Wang, Hong Wang, Xudong Zhao, Muhammad Aamer Mehmood, Hui Zhu
Summary: An efficient acetic-acid-tolerant P. kudriavzevii strain, Y2, was isolated from strong-flavored baijiu brewing waste, and its molecular mechanism of acetic acid tolerance was investigated. The results showed that differentially expressed genes were mainly enriched in oxidative phosphorylation, the citrate cycle, glycolysis/gluconeogenesis, and carbon metabolism pathways. The study provided a basis for further investigation of the acid tolerance mechanism in P. kudriavzevii.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Huiping Tan, Liang Wang, Huiguo Wang, Yanghao Cheng, Xiang Li, Huihui Wan, Chenguang Liu, Tian Liu, Qian Li
Summary: In this study, Komagataella phaffii was engineered to produce cordycepin from methanol, reaching a high productivity of 15.95 mg/(L center dot h). The study also identified a deaminated product of cordycepin during fermentation. This research provides a novel platform for cordycepin production and practical strategies for microbial cell factory optimization.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Article
Engineering, Environmental
Tian-Jie Ao, Xin-Qing Zhao, Muhammad Aamer Mehmood, Ning Wang, Hui Zhu, Chen-Guang Liu, Feng-Wu Bai
Summary: This study investigated the effects of electrodes on the performance of anaerobic digestion (AD) by using a double-chamber microbial electrolysis cell integrated anaerobic digestion system (MEC-AD) fed with corn stover. It was found that methane yield was enhanced by 80.41% at 0.6 V, but there was a sharp decline in methanogens at 0.9 V, leading to acidification. The synergistic stabilization of both anode and cathode chambers resulted in the overall improvement of the MEC-AD system.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Multidisciplinary Sciences
ChenGuang Liu, JinZhong Fu, QingFeng Hua, Kai Liu, PengYao Zhi, YanLiang Pei, QingJie Zhou, XiShuang Li, BaoHua Liu
Summary: A new method was employed in a geophysical survey of the Ninety East Ridge of the Indian Ocean to perform proportional double seismic source excitation and simultaneously receive signals from the sea surface and the seabed. The survey used two sets of seismic sources with different energies and dominant frequencies, G gun arrays and Bolt gun arrays, and received signals using a 480-channel seismic streamer towed from the sea surface and 21 ocean bottom seismometers (OBS). By processing the data obtained, deep seismic profiles from both the Bolt gun seismic source and the active-source OBS survey were obtained along with the conventional G gun seismic source profile, greatly improving the efficiency of offshore deep-sea seismic surveys.
SCIENTIFIC REPORTS
(2023)
