Journal
BIOTECHNOLOGY JOURNAL
Volume 7, Issue 5, Pages 662-666Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201100453
Keywords
Methods; Rayleigh-Benard convective PCR; Thermally baffled PCR instrument; Yellow head virus
Funding
- Taiwan National Science Council (NSC) [NSC 99-2112-M-005-006-MY3]
- NSC-Central Taiwan Science Park [NSC-CTSP 302403501]
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Rayleigh-Benard convective PCR is a simple and effective design for amplification of DNA. Convective PCR is, however, extremely sensitive to environmental temperature fluctuations, especially when using small- diameter test tubes. Therefore, this method is inherently unstable with limited applications. Here, we present a convective PCR device that has been modified by adding thermal baffles. With this thermally baffled device the influence from fluctuations in environmental temperature were significantly reduced, even in a wind tunnel (1 m/s). The thermally baffled PCR instrument described here has the potential to be used as a low-cost, point-of-care device for PCR-based molecular diagnostics in the field.
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